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Chapter 5:
Transport in Plants
Learning Objectives
Identify positions of xylem and
phloem in a dicot leaf, stem and
root
State the structures of xylem
and phloem & relate to their
functions
Transport of substances
How blood gets
transported in
our body?
How water, food
and minerals gets
transported in
Plants?
Imagine…
What transports water from the roots to the furthest
leaf?
Did You Know That?!
- Average of 60 meters in height
- A rainforest tree uses ~1200 L
of water/day
What do plants rely on?
The bottle tree plant,
‘Baobab’ that live 500 years and store up 300 litre of
water.
• BBC Video:
http://www.youtube.com/watch?v=M9V9bVEDSVs
• http://www.youtube.com/watch?v=U2iFl6lvj4c
Xylem
• Continuous long, hollow tube
• Dead, without protoplasm or
cross-walls
• Walls are deposited with
lignin, which is a hard and
rigid substance
Drawing
UndertheMicroscope
Describe what you see in the picture…
In flowering plants, the transport tissue consists of the
xylem and the phloem.
Xylem
Transports
&
Xylem
Function
Water Minerals
Lignin • Walls are strengthened with
lignin, which is a hard and
rigid substance
Xylem
Mechanical support
Phloem
Describe what you see in the picture…
• It is a living structure
• Made up of sieve tubes and
companion cells
• Transport food manufactured
in the leaves to other parts of
the plant by translocation
Phloem
(Sucrose)
• Food is either transported
or diffused through the
cytoplasm of the sieve tube
cells.
• Each sieve tube has a
companion cell beside it to:
 keep it alive
 provide energy for
active transport
Phloem
transportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportin
Function: Adaptations:
Transport
manufactured
food from the
leaves to other
parts of the
plants.
• Companion cells have
lots of mitochondria
which provides the
energy needed by the
sieve tube cells for
respiration.
• Holes in the sieve plates
allow transport of
manufactured food
substances through the
sieve tubes.
Phloem
Translocation (Phloem)
How do we know that the phloem transport
manufactured food??
translocationSTUDIES (pure)
Isotopes• The plant is grown in the
environment containing radioactive
carbon dioxide 14CO2.
• When photosynthesis takes place, the
sugar formed will contain radioactive
carbon.
• The cut stem section when exposed
onto an X-ray photographic film will
turn cloudy.
• This shows that the radioactive
substances are present in the phloem.
Aphids• Aphids are parasites that feed
on plants.
• Mouthparts (proboscis) of
aphids penetrate the leaf or stem
during feeding.
• When the body of the aphid is
cut off, leaving behind the
proboscis in the plant tissues, the
liquid will exude from the cut end
of the proboscis.
• The liquid is found to contain
sucrose and amino acids.
http://www.youtube.com/watch?v=J7eRGH
Vx3p0
translocationSTUDIES (pure)
Ringing experiment1. Removing a ring of bark
from a woody branch will
result in the removal of the
phloem tissue in that region.
Swelling will be observed in
the region above the ring.
2. The swelling above the
ring where the phloem is
removed will be caused by
an accumulation of sugars in
the region above the ring.
A
B
region with bark
removed
translocationSTUDIES (pure)
Translocation studies can be carried out using aphids. The
diagram below shows a section of a plant stem where the
proboscis of a feeding aphid may be found.
In which tissue would the proboscis most likely be inserted
into?
microQUESTION9.4
A
D
C
B
Vascular Bundle
• Xylem vessels + Phloem vessels  Vascular Bundle
• Found in the…
Root
Stem
Leaf
Magnification: 100X
Vascular Bundle of a Sunflower root
Vascular bundle
Root
Xylem
Phloem
Magnification: 100X
Phloem
Xylem
Vascular Bundle of a Sunflower Stem
Vascular bundle
Stem
vascularBUNDLE(pure)
XYLEM
PHLOEM
CAMBIUM
The xylem and the phloem group together to form a
vascular bundle (literally a bundle of vessels).
Magnification: 400X
Vascular bundle
Leaf
Xylem
Phloem
Transport in Plants
Xylem Phloem
Water
Mineral
Salt
Mechanical
Support
Lignified
Walls
Manufactured
food (Sucrose)
Sieve
tube
Companion
cell
Uptake of Water
30
Uptake of Water by the Roots
31
Movement of Water molecules
Osmosis is the net movement of water molecules
from a solution of higher water potential to a solution of
lower water potential through a selectively permeable
membrane
32
waterTRANSPORT
Root Hair Cell
Adaptation Function
Root hair cell has long
& narrow protrusions
Increase surface area to
volume ratio for faster rate of
absorption of water and mineral
salts
Cell surface
membrane prevents
leaking of cell sap
Maintain low water potential of
cell for osmosis
Living cell Provides energy from cellular
respiration for absorption of
mineral salts by active transport
Root Hair Cell Structure &
Function
Taking the train…
35
Please Consider the
following…
36
Uptake of water by Root Hair Cells
Xylem 37
How is water
transported from
the xylem in the
roots to the leaves?
38
In plants…
Plants only use about
5 to 10% of the
water they uptake
…for plant
growth and
cellular use
39
Eg. If a huge tree takes in
1200L of water a day and
only uses 10%...
Where did the other 90%
went to?
40
Water is loss to the environment mostly
through the?....
42
Let take a look at the cross section
of a leaf…
43
A large
percentage of
water that is
absorbed is lost
by evaporation
through the
stomata
44
This process of
water vapour
being lost
mainly through
the stomata is
known as
transpiration
45
How does
transpiration help
to bring water in
the roots to the
leaves?
46
Properties of water H
HO
Cohesive….’Attracts to one another’
Adhesive….’Attracts to other substances’
47
This creates a
suction force
which pulls
water up the
xylem vessels
This suction force due to
transpiration is known as
transpiration pull
48
In the stem, water moves
up the xylem primarily
via transpiration pull
Transport in Plants
50
51
Purpose of Transpiration
Cools the leaves
Transport water &
minerals from the
roots to the leaves
Explain the factors affecting
the rate of transpiration
Learning Objectives
52
Factors Affecting Rate of Transpiration
• Temperature
• Humidity
• Wind
• Light
53
Which of the following correctly states the pathway in which
water molecules leave a plant during transpiration?
A ) Palisade mesophyll  vascular bundle  intercellular air
spaces  stomata
B ) Spongy mesophyll  intercellular air spaces  stomata 
epidermis  cuticle
C ) Vascular bundle  spongy mesophyll  intercellular air
spaces  stomata
D ) Vascular bundle  intercellular air spaces  stomata 
epidermis  cuticle ( )
microQUESTION9.8
Measuring Rate of
Transpiration
Experiment set-up to measure the rate of transpiration
(1) (2)
• In set-up (1), the loss of water by
evaporation is avoided by adding a layer
of oil on the surface of the water.
• Water is lost through the leaves of the
plant and will result in the mass of the
test tube decreasing.
Rate of transpiration = Loss in mass (g)
(g/h) Time taken (h)
• Set-up (2), known as “potometer”.
• The suction force generated by
transpiration will cause the air bubble in
the graduated capillary tube to move to
the left to indicate volume of water
absorbed to replace water loss.
Rate of transpiration = Loss in volume (cm3)
(cm3/h) Time taken (h)
57
58
59
Distance
moved
Adaptation of ‘Marram grass’
• http://www.youtube.com/watch?v=Dv1-NDw49Vw
The transpiration rate of a plant varies throughout the day,
depending on the environmental conditions. Which of the
following sets of environmental conditions would result in the
highest transpiration rate?
microQUESTION9.9
Temperature Cloud cover Humidity Wind speed
A High Maximum High High
B Low Minimal Low Low
C High Minimal Low High
D Low Maximum High Low
( )
Wilting
Wilting
Excessive transpiration causes
a plant to wilt.
Wilting occurs when a plant
loses more water through the
leaves, than it absorbs through
the roots.
Wilting• Turgor pressure in the
mesophyll cells in the leaf
helps to support the leaf and
keep the leaf firm and widely
spread out to absorb sunlight
for photosynthesis.
• In strong sunlight, excess
transpiration causes cells to
lose turgor pressure and
become flaccid.
Wilting
Excessive Transpiration
Guard cells
become flaccid
* Rate of photosynthesis
decrease
Stomata close
Wilting- Advantage
• Less surface area exposed to sunlight
• Guard cells  flaccid  Stomata closes
• Transpiration is reduced
Wilting- Disadvantage
• Guard cells become flaccid  Stomata closes
• Carbon Dioxide intake is reduced
• Rate of Photosynthesis decreases

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Transport in plants

  • 2. Learning Objectives Identify positions of xylem and phloem in a dicot leaf, stem and root State the structures of xylem and phloem & relate to their functions
  • 3. Transport of substances How blood gets transported in our body? How water, food and minerals gets transported in Plants?
  • 4. Imagine… What transports water from the roots to the furthest leaf? Did You Know That?! - Average of 60 meters in height - A rainforest tree uses ~1200 L of water/day What do plants rely on?
  • 5. The bottle tree plant, ‘Baobab’ that live 500 years and store up 300 litre of water. • BBC Video: http://www.youtube.com/watch?v=M9V9bVEDSVs • http://www.youtube.com/watch?v=U2iFl6lvj4c
  • 6. Xylem • Continuous long, hollow tube • Dead, without protoplasm or cross-walls • Walls are deposited with lignin, which is a hard and rigid substance Drawing UndertheMicroscope Describe what you see in the picture…
  • 7. In flowering plants, the transport tissue consists of the xylem and the phloem. Xylem
  • 9. Lignin • Walls are strengthened with lignin, which is a hard and rigid substance Xylem Mechanical support
  • 10. Phloem Describe what you see in the picture…
  • 11. • It is a living structure • Made up of sieve tubes and companion cells • Transport food manufactured in the leaves to other parts of the plant by translocation Phloem (Sucrose)
  • 12. • Food is either transported or diffused through the cytoplasm of the sieve tube cells. • Each sieve tube has a companion cell beside it to:  keep it alive  provide energy for active transport Phloem
  • 13. transportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportinplantstransportin Function: Adaptations: Transport manufactured food from the leaves to other parts of the plants. • Companion cells have lots of mitochondria which provides the energy needed by the sieve tube cells for respiration. • Holes in the sieve plates allow transport of manufactured food substances through the sieve tubes. Phloem
  • 14. Translocation (Phloem) How do we know that the phloem transport manufactured food??
  • 15. translocationSTUDIES (pure) Isotopes• The plant is grown in the environment containing radioactive carbon dioxide 14CO2. • When photosynthesis takes place, the sugar formed will contain radioactive carbon. • The cut stem section when exposed onto an X-ray photographic film will turn cloudy. • This shows that the radioactive substances are present in the phloem.
  • 16. Aphids• Aphids are parasites that feed on plants. • Mouthparts (proboscis) of aphids penetrate the leaf or stem during feeding. • When the body of the aphid is cut off, leaving behind the proboscis in the plant tissues, the liquid will exude from the cut end of the proboscis. • The liquid is found to contain sucrose and amino acids. http://www.youtube.com/watch?v=J7eRGH Vx3p0 translocationSTUDIES (pure)
  • 17. Ringing experiment1. Removing a ring of bark from a woody branch will result in the removal of the phloem tissue in that region. Swelling will be observed in the region above the ring. 2. The swelling above the ring where the phloem is removed will be caused by an accumulation of sugars in the region above the ring. A B region with bark removed translocationSTUDIES (pure)
  • 18. Translocation studies can be carried out using aphids. The diagram below shows a section of a plant stem where the proboscis of a feeding aphid may be found. In which tissue would the proboscis most likely be inserted into? microQUESTION9.4 A D C B
  • 19. Vascular Bundle • Xylem vessels + Phloem vessels  Vascular Bundle • Found in the… Root Stem Leaf
  • 21. Vascular Bundle of a Sunflower root
  • 26. vascularBUNDLE(pure) XYLEM PHLOEM CAMBIUM The xylem and the phloem group together to form a vascular bundle (literally a bundle of vessels).
  • 29. Transport in Plants Xylem Phloem Water Mineral Salt Mechanical Support Lignified Walls Manufactured food (Sucrose) Sieve tube Companion cell
  • 31. Uptake of Water by the Roots 31
  • 32. Movement of Water molecules Osmosis is the net movement of water molecules from a solution of higher water potential to a solution of lower water potential through a selectively permeable membrane 32
  • 34. Adaptation Function Root hair cell has long & narrow protrusions Increase surface area to volume ratio for faster rate of absorption of water and mineral salts Cell surface membrane prevents leaking of cell sap Maintain low water potential of cell for osmosis Living cell Provides energy from cellular respiration for absorption of mineral salts by active transport Root Hair Cell Structure & Function
  • 37. Uptake of water by Root Hair Cells Xylem 37
  • 38. How is water transported from the xylem in the roots to the leaves? 38
  • 39. In plants… Plants only use about 5 to 10% of the water they uptake …for plant growth and cellular use 39
  • 40. Eg. If a huge tree takes in 1200L of water a day and only uses 10%... Where did the other 90% went to? 40
  • 41.
  • 42. Water is loss to the environment mostly through the?.... 42
  • 43. Let take a look at the cross section of a leaf… 43
  • 44. A large percentage of water that is absorbed is lost by evaporation through the stomata 44
  • 45. This process of water vapour being lost mainly through the stomata is known as transpiration 45
  • 46. How does transpiration help to bring water in the roots to the leaves? 46
  • 47. Properties of water H HO Cohesive….’Attracts to one another’ Adhesive….’Attracts to other substances’ 47
  • 48. This creates a suction force which pulls water up the xylem vessels This suction force due to transpiration is known as transpiration pull 48 In the stem, water moves up the xylem primarily via transpiration pull
  • 49.
  • 51. 51 Purpose of Transpiration Cools the leaves Transport water & minerals from the roots to the leaves
  • 52. Explain the factors affecting the rate of transpiration Learning Objectives 52
  • 53. Factors Affecting Rate of Transpiration • Temperature • Humidity • Wind • Light 53
  • 54. Which of the following correctly states the pathway in which water molecules leave a plant during transpiration? A ) Palisade mesophyll  vascular bundle  intercellular air spaces  stomata B ) Spongy mesophyll  intercellular air spaces  stomata  epidermis  cuticle C ) Vascular bundle  spongy mesophyll  intercellular air spaces  stomata D ) Vascular bundle  intercellular air spaces  stomata  epidermis  cuticle ( ) microQUESTION9.8
  • 56. Experiment set-up to measure the rate of transpiration (1) (2) • In set-up (1), the loss of water by evaporation is avoided by adding a layer of oil on the surface of the water. • Water is lost through the leaves of the plant and will result in the mass of the test tube decreasing. Rate of transpiration = Loss in mass (g) (g/h) Time taken (h) • Set-up (2), known as “potometer”. • The suction force generated by transpiration will cause the air bubble in the graduated capillary tube to move to the left to indicate volume of water absorbed to replace water loss. Rate of transpiration = Loss in volume (cm3) (cm3/h) Time taken (h)
  • 57. 57
  • 58. 58
  • 60. Adaptation of ‘Marram grass’ • http://www.youtube.com/watch?v=Dv1-NDw49Vw
  • 61. The transpiration rate of a plant varies throughout the day, depending on the environmental conditions. Which of the following sets of environmental conditions would result in the highest transpiration rate? microQUESTION9.9 Temperature Cloud cover Humidity Wind speed A High Maximum High High B Low Minimal Low Low C High Minimal Low High D Low Maximum High Low ( )
  • 63. Wilting Excessive transpiration causes a plant to wilt. Wilting occurs when a plant loses more water through the leaves, than it absorbs through the roots.
  • 64. Wilting• Turgor pressure in the mesophyll cells in the leaf helps to support the leaf and keep the leaf firm and widely spread out to absorb sunlight for photosynthesis. • In strong sunlight, excess transpiration causes cells to lose turgor pressure and become flaccid.
  • 65. Wilting Excessive Transpiration Guard cells become flaccid * Rate of photosynthesis decrease Stomata close
  • 66. Wilting- Advantage • Less surface area exposed to sunlight • Guard cells  flaccid  Stomata closes • Transpiration is reduced
  • 67. Wilting- Disadvantage • Guard cells become flaccid  Stomata closes • Carbon Dioxide intake is reduced • Rate of Photosynthesis decreases