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Conventional methods of remediation
   Conventional methods of remediation
   Dig up and remove it to a landfill Cap
    and contain
   Risk of excavation, handling and
   transport of hazardous material
   Very expensive to find another land to
    finally dispose these materials
   Maintain it in the same land but
    isolate it
Only an interim solution
 Requires monitoring and
 maintenance of isolation
 barriers for a long time
Is there a better approach?
Products are not converted
 into harmless products. Stay
 as a threat!
Better approaches :
Destroy them completely, if
 possible Transform them in to
 harmless substances
 High temperature incineration
Chemical decomposition like
 dechlorination,
 UV oxidation Methods already
 in use
Bioremediation makes
 effective better approach
 possible. Either by
 destroying or render them
 harmless using natural
 biological activity.
 Relatively low cost
 Low technology techniques
 Generally has general public acceptance
 Can often be carried out on site – no
  excavation, no transport
 Drawbacks
 May not be effective on all contaminants
 Time duration – relatively long
 Expertise required to design and
  implement – although not technically
  complex
Bioremediation :
is defined as the process
 whereby organic wastes are
 biologically degraded under
 controlled conditions to an
 innocuous state, or to levels
 below concentration limits
 established by regulatory
 authorities
Types of Bioremediation :




 Engineered Bioremediation


  Intrinsic Bioremediation
Qualities of :

 Qualities of Microorganisms
  Environment
 Able to degrade hydrocarbons
 Able to fix nitrogen No secondary/side
  effects
 Presence of accessory nutrients (N P K
  Fe)
 Absence of heavy metals Adequate
 O2, Temperature, pH
Sources of microorganisms :
 From contaminated field sites (with
  varying environmental conditions -
  subzero temperatures or extreme
  heat, desert conditions or in water, with
  excess of oxygen or in anaerobic
  conditions, with presence of hazardous
  compounds or on any waste stream)
 From culture collections Genetically
  Engineered Microorganisms (GEMs)
 Sources of microorganisms
Bioremediation Strategies :

Bioremediation
 Strategies
 In situ Bioremediation
 (at the site)
Ex situ Bioremediation
 (away from the site)
Bioaugmentation involves
 practice of adding specialized
 microbes or their enzyme
 preparation to polluted
 matrices to accumulate
 transformation or stabilization
 of specific pollutants
Ex situ bioremediation types :

Ex situ bioremediation types
 Landfarming involves a simple
 technique in which
 contaminated soil is excavated
 and spread over a prepared
 bed and periodically tilled until
 pollutants are degraded.
Phytoextraction :


Phytoextraction Also called
 Phytoaccumulation
A process used by the plants to
 accumulate contaminants into
 the roots and shoots or leaves.
Phytotransformation :
Phytotransformation also called
 Phytodegradation refers to the
 uptake of organic contaminants
 from soil, sediments, or water
 and, subsequently, their
 transformation to more stable, less
 toxic, or less mobile form. Metal
 chromium can be reduced from
 hexavalent to trivalent
 chromium, which is a less mobile
 and non-carcinogenic form.

Phytostabilization :

Phytostabilization Leachable
 (permeate gradually)
 constituents are adsorbed and
 bound into the plant structure
 so that they form a stable mass
 of plant from which the
 contaminants will not reenter
 the environment
Phytodegradation :
 Phytodegradation also called
  rhizodegradation is the breakdown of
  contaminants through the activity existing
  in the rhizosphere. Due to the presence of
  proteins and enzymes produced by the
  plants or by soil organisms such as
  bacteria, yeast, and fungi. a symbiotic
  relationship that has evolved between
  plants and microbes Plants provide
  nutrients necessary for the microbes to
  thrive, while microbes provide a healthier
  soil environment.
Rhizofiltration :

Rhizofiltration is a water
 remediation technique that
 involves the uptake of
 contaminants by plant roots used
 to reduce contamination in
 natural wetlands and estuary
 areas The wide part of a river
 where it nears the sea; fresh and
 salt water mix
Phytoremediation is well suited
for :
   Phytoremediation is well suited for use
    at very large field sites where other
    methods of remediation are not cost
    effective or practicable use at sites
    with a low concentration of
    contaminants where only polish
    treatment is required over long periods
    of time in conjunction with other
    technologies where vegetation is used
    as a final cap and closure of the site
Thank you

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Bioremediation

  • 1.
  • 2. Conventional methods of remediation  Conventional methods of remediation  Dig up and remove it to a landfill Cap and contain  Risk of excavation, handling and transport of hazardous material  Very expensive to find another land to finally dispose these materials  Maintain it in the same land but isolate it
  • 3. Only an interim solution  Requires monitoring and maintenance of isolation barriers for a long time Is there a better approach? Products are not converted into harmless products. Stay as a threat!
  • 4. Better approaches : Destroy them completely, if possible Transform them in to harmless substances  High temperature incineration Chemical decomposition like dechlorination,  UV oxidation Methods already in use
  • 5. Bioremediation makes effective better approach possible. Either by destroying or render them harmless using natural biological activity.
  • 6.  Relatively low cost  Low technology techniques  Generally has general public acceptance  Can often be carried out on site – no excavation, no transport  Drawbacks  May not be effective on all contaminants  Time duration – relatively long  Expertise required to design and implement – although not technically complex
  • 7. Bioremediation : is defined as the process whereby organic wastes are biologically degraded under controlled conditions to an innocuous state, or to levels below concentration limits established by regulatory authorities
  • 8. Types of Bioremediation :  Engineered Bioremediation Intrinsic Bioremediation
  • 9. Qualities of :  Qualities of Microorganisms Environment  Able to degrade hydrocarbons  Able to fix nitrogen No secondary/side effects  Presence of accessory nutrients (N P K Fe)  Absence of heavy metals Adequate O2, Temperature, pH
  • 10. Sources of microorganisms :  From contaminated field sites (with varying environmental conditions - subzero temperatures or extreme heat, desert conditions or in water, with excess of oxygen or in anaerobic conditions, with presence of hazardous compounds or on any waste stream)  From culture collections Genetically Engineered Microorganisms (GEMs)  Sources of microorganisms
  • 11. Bioremediation Strategies : Bioremediation Strategies  In situ Bioremediation (at the site) Ex situ Bioremediation (away from the site)
  • 12. Bioaugmentation involves practice of adding specialized microbes or their enzyme preparation to polluted matrices to accumulate transformation or stabilization of specific pollutants
  • 13. Ex situ bioremediation types : Ex situ bioremediation types Landfarming involves a simple technique in which contaminated soil is excavated and spread over a prepared bed and periodically tilled until pollutants are degraded.
  • 14. Phytoextraction : Phytoextraction Also called Phytoaccumulation A process used by the plants to accumulate contaminants into the roots and shoots or leaves.
  • 15. Phytotransformation : Phytotransformation also called Phytodegradation refers to the uptake of organic contaminants from soil, sediments, or water and, subsequently, their transformation to more stable, less toxic, or less mobile form. Metal chromium can be reduced from hexavalent to trivalent chromium, which is a less mobile and non-carcinogenic form. 
  • 16. Phytostabilization : Phytostabilization Leachable (permeate gradually) constituents are adsorbed and bound into the plant structure so that they form a stable mass of plant from which the contaminants will not reenter the environment
  • 17. Phytodegradation :  Phytodegradation also called rhizodegradation is the breakdown of contaminants through the activity existing in the rhizosphere. Due to the presence of proteins and enzymes produced by the plants or by soil organisms such as bacteria, yeast, and fungi. a symbiotic relationship that has evolved between plants and microbes Plants provide nutrients necessary for the microbes to thrive, while microbes provide a healthier soil environment.
  • 18. Rhizofiltration : Rhizofiltration is a water remediation technique that involves the uptake of contaminants by plant roots used to reduce contamination in natural wetlands and estuary areas The wide part of a river where it nears the sea; fresh and salt water mix
  • 19. Phytoremediation is well suited for :  Phytoremediation is well suited for use at very large field sites where other methods of remediation are not cost effective or practicable use at sites with a low concentration of contaminants where only polish treatment is required over long periods of time in conjunction with other technologies where vegetation is used as a final cap and closure of the site