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RTB Foresight in Breeding
MICHAEL FRIEDMANN • FORESIGHT IN BREEDING, ROME 2019
Banana
Plantain
Cassava Potato Sweetpotato Yam Other R&T
Our crops
300 million small holder farmers , their families and
processors depend on RTB crops
buffering role in food systems
Steps in climate smart breeding
1. Looking into the future: downscaling climate change
models & crop modelling - drivers of yield loss
2. Identifying key traits to respond to drivers and
estimating trait level needed to respond to climate
change- incorporate into product profiles
3. Medium term: varietal selection for best bet climate
smart varieties-with end-user needs and preferences
4. Long term: genomic research and high thru-put
phenotyping to incorporate climate responsive traits
5. Developing management options for climate smart
varieties
6. Deployment (seed system)
draft product profiles
Crop Trait Priority GMO GWAS GS QTL
molecular
markers
Diagnostic
Banana/plantain Yield 1
Highlands East & R to black leaf streak, Sigatoka 2 to 3
Central Africa R to Banana bunchy top virus, 3
R to banana weevil 2
R to Fusarium wilt 1 R1 SR4
R to nematodes (various species) 2
R to Xanthomonas wilt 2
Early maturity 2
dwarf types, plant height 1 to 2
plant stature 2
Suckering behavior 2
Drought tolerance 3
Fruit parthenocarpy 1
Table quality (texture, flavor, and color) 1 Mataoke
Bunch orientation 1
Bunch compactness 2
High Pro Vit A content 2
Crop Trait Priority G
Potato Yield 1
Mid-elevation tropics Table quality (appearance, cooking types,
glycoalkaloid content)
1
Earliness 1
High Fe or Zn 3
R to Late blight 1
Drought tolerance 2
R to PVY 1
Heat tolerance 3
Chipping ability 1
R to Bacterial Wilt 3
R to PLRV 3
R to PVX 3
potatobanana
Climate resilience breeding targets
Crop Opt. temp (oC) Max temp (o
C) drought period breeding
banana 25-27/18 31-38 (strong damage
and wilting)
2-3 months reach 33 (o
C) day temp
cassava >20; phtsis 25-35 sprouting ceases >37 bulking stops, but resumes.
Grows from 600 to 1000mm
bulking to continue under drought; less
remobilization of starch when moisture returns-
erratic rainfall patterns
potato /10-20 night 28/18-20 go over Tmax (21-22o
C night), short season 70-
80 d
Sweetpotato 25-30/15-20 25-30 (27.2) day temp critical for planting material landraces with high potential
yam 25-30 30 26-29 days WUE and NUE linked
Raising temperature limits,
increased drought tolerance,
context-specific, and not enough
heat effects on anti-
nutritional compounds
Extreme weather events-
flooding in Rwanda- sweetpotato
as security crop 70 – 80
DAYS
10
14
18
22
26
30
34
38
Oct Nov Dec Jan Feb Mar
(Midmore, 1992)
AirTemperatureºC
Max Day Tº
Max Night Tº
GWAS for potato yield components under
warm long day conditions
5 9
Mihovilovich & Bonierbale, CIP
Sweetpotato screen for heat tolerance
Thermographic image: field in summer
2014 - heat stress exposure at maximum
storage root bulking
Yield of storage roots vs. pencil roots represent
an indicator for heat tolerance
Considerable genetic variation heat stress tolerance
Climate change: pests
and diseases
LB severity—current LB severity—2050 Legend
Figure 5. Predictions of potato late blight severity in sub-Saharan Africa using a metamodel (source: Sparks et al
2014).
Climate
proofing seed
systems
RTB Scaling Fund
• Design & implementation of
scaling strategies for most
promising RTB innovations
• Semi-competitive
• Cross-team collaboration
• Complementarities with other
funds
• Proposals
• USD 200 – 500 K
• 12 to 24 months
Scaling in RTB
Multi-CRP package to address resilience &
adaptation of RTB systems to climate change
• low cost irrigation -RTB, WLE, CCAFS, PIM
• For seed systems, yield gaps, climate resilience
• Expand production in sub-Sahel region for cassava, sweetpotato.
• Protect banana production
iDE Hero Ghana Watering Can
https://wle.cgiar.org/solutions/groundwater

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Breeding foresight workshop: Presentation by CGIAR RTB

  • 1. F e b r u a r y 2 0 1 9 RTB Foresight in Breeding MICHAEL FRIEDMANN • FORESIGHT IN BREEDING, ROME 2019
  • 2. Banana Plantain Cassava Potato Sweetpotato Yam Other R&T Our crops 300 million small holder farmers , their families and processors depend on RTB crops buffering role in food systems
  • 3. Steps in climate smart breeding 1. Looking into the future: downscaling climate change models & crop modelling - drivers of yield loss 2. Identifying key traits to respond to drivers and estimating trait level needed to respond to climate change- incorporate into product profiles 3. Medium term: varietal selection for best bet climate smart varieties-with end-user needs and preferences 4. Long term: genomic research and high thru-put phenotyping to incorporate climate responsive traits 5. Developing management options for climate smart varieties 6. Deployment (seed system)
  • 4. draft product profiles Crop Trait Priority GMO GWAS GS QTL molecular markers Diagnostic Banana/plantain Yield 1 Highlands East & R to black leaf streak, Sigatoka 2 to 3 Central Africa R to Banana bunchy top virus, 3 R to banana weevil 2 R to Fusarium wilt 1 R1 SR4 R to nematodes (various species) 2 R to Xanthomonas wilt 2 Early maturity 2 dwarf types, plant height 1 to 2 plant stature 2 Suckering behavior 2 Drought tolerance 3 Fruit parthenocarpy 1 Table quality (texture, flavor, and color) 1 Mataoke Bunch orientation 1 Bunch compactness 2 High Pro Vit A content 2 Crop Trait Priority G Potato Yield 1 Mid-elevation tropics Table quality (appearance, cooking types, glycoalkaloid content) 1 Earliness 1 High Fe or Zn 3 R to Late blight 1 Drought tolerance 2 R to PVY 1 Heat tolerance 3 Chipping ability 1 R to Bacterial Wilt 3 R to PLRV 3 R to PVX 3 potatobanana
  • 5. Climate resilience breeding targets Crop Opt. temp (oC) Max temp (o C) drought period breeding banana 25-27/18 31-38 (strong damage and wilting) 2-3 months reach 33 (o C) day temp cassava >20; phtsis 25-35 sprouting ceases >37 bulking stops, but resumes. Grows from 600 to 1000mm bulking to continue under drought; less remobilization of starch when moisture returns- erratic rainfall patterns potato /10-20 night 28/18-20 go over Tmax (21-22o C night), short season 70- 80 d Sweetpotato 25-30/15-20 25-30 (27.2) day temp critical for planting material landraces with high potential yam 25-30 30 26-29 days WUE and NUE linked Raising temperature limits, increased drought tolerance, context-specific, and not enough heat effects on anti- nutritional compounds Extreme weather events- flooding in Rwanda- sweetpotato as security crop 70 – 80 DAYS 10 14 18 22 26 30 34 38 Oct Nov Dec Jan Feb Mar (Midmore, 1992) AirTemperatureºC Max Day Tº Max Night Tº
  • 6. GWAS for potato yield components under warm long day conditions 5 9 Mihovilovich & Bonierbale, CIP
  • 7. Sweetpotato screen for heat tolerance Thermographic image: field in summer 2014 - heat stress exposure at maximum storage root bulking Yield of storage roots vs. pencil roots represent an indicator for heat tolerance Considerable genetic variation heat stress tolerance
  • 8. Climate change: pests and diseases LB severity—current LB severity—2050 Legend Figure 5. Predictions of potato late blight severity in sub-Saharan Africa using a metamodel (source: Sparks et al 2014).
  • 10. RTB Scaling Fund • Design & implementation of scaling strategies for most promising RTB innovations • Semi-competitive • Cross-team collaboration • Complementarities with other funds • Proposals • USD 200 – 500 K • 12 to 24 months Scaling in RTB
  • 11. Multi-CRP package to address resilience & adaptation of RTB systems to climate change • low cost irrigation -RTB, WLE, CCAFS, PIM • For seed systems, yield gaps, climate resilience • Expand production in sub-Sahel region for cassava, sweetpotato. • Protect banana production iDE Hero Ghana Watering Can https://wle.cgiar.org/solutions/groundwater

Hinweis der Redaktion

  1. Genome Wide Association studies were performed to analyze the genetic architecture for traits associated to tuberization (tuber induction) and bulking under warm and long daylength conditons in 171 advanced breeding lines from CIP potato genotyped with 4738 SolCAP SNP. breeding program . Breeding lines were phenotyped in field plots in the lowland subtropics (Lima, Perú; 12° 3' 0" S), using high pressure sodium vapor lamps to extend the photoperiod to 16 H. Tuber induction as a measure of tuber initiation was evaluated 40 days after plant emergency using the single node cutting method in which stem cuttings containing a bud are taken from field mother plants, planted in a rooting substrate Jiffy 7 and keep for 15 days with high humidity , 20 h light and 28 C , time after which cuttings are assessed in a 1 (no induction) and 9 (strong induction) for tuber formation in the bud. When a shoot is developed from the bud means that the plant is not yet induced to tuberize, while it is induced if a tuber or a stolon develop from the bud. Stolon length was measured in a 1(the shortest) -9 (the longest) scale tuber bulking was estimated as the (number of marketable tubers / number of total tubers) * proportion of tuberized plants*100 (expressed on % of bulking) . Distribution of breeding lines for each trait is shown in the left. Scale for assessment of tuber initiation and stolon length at the center and GWAS charts indicating significant SNP associated with these traits in the center and right. Significant SNP associated with bulking are shown in Chr 2 and 4 , with tuber induction on chr 2 , 3, 4, 5 and for stolon length on chr 2, 4, 5, and 6 . (chr=13 are SNP markers that were not able to be mapped in the potato genome)
  2. solar-powered drip irrigation significantly augments both household income and nutritional intake, particularly during the dry season, and is cost effective compared to alternative technologies