1) The document discusses analytical tools used to evaluate algae, including Nile red staining, thin layer chromatography, HPLC/MS, and GC/MS. These tools can provide more accurate measurements of lipids than traditional methods. 2) Technologies developed at UT/OpenAlgae allow individual measurement of lipids and tracking of fatty acids like omega-3 throughout growth and processing. This helps increase understanding of algal lipid dynamics. 3) Preliminary results suggest algae extracts with omega-7 can accelerate wound closure, improve blood flow, and minimize scarring in wounded sheep. Further studies and industry collaboration may develop health applications.

1. Algae Analytics and Nutraceutical Applications for Algae Rhykka Connelly r.connelly@cem.utexas.edu April 27, 2011

2. Standard Measurement Technologies Nile Red is the current measurement tool of choice in the algae industry Here’s what Nile red measures: lipophilic molecules Here’s an example of how Nile red measurements can mislead algae growers: Nile red measurements may indicate that both of these samples has 30% “oil”

3. UT/OpenAlgae Technologies Evaluating the intermediate products requires many analytical tools

4. UT/OpenAlgae Technologies – Thin Layer Chromatography Samples are taken before and after each processing step. Each sample is separated into a biomass pellet (P) and supernatant (S) fraction and analyzed by TLC against known standards.

9. UT sets itself apart by incorporating cutting edge technologies that measure lipids individually

11. Certain kinds of algae make products that we’re interested in

12. oil for fuel

13. high in proteins and/or carbohydrates (animal or aquaculture feeds)

14. agar (thickener)

15. carrageenan (stabilizer/emulsifier)

17. We have identified an algae that produces large quantities of Omega-7 and can be harvested daily. The problem: Limited vascularization at the wound site

18. Omega Oils – Commercially Available Omega 7 Wound Healing Study Preliminary Results Wound Closure Omega-7 accelerates wound closure

19. Omega Oils – Commercially Available Omega 7 Wound Healing Study Preliminary Results Blood Flow Due to Neovascularization Scar Formation Omega-7 extracts improve blood flow and minimizes scar formation in wounded sheep.

20. Omega 7 – A Collaborative Approach Algae Scale-up and Processing In Vitro and in Vivo Study The results of the study will be published, and if successful, be extended to industry.

22. Circumference

23. Number of fruits/leaves produced

24. Soil analysesAlgae Commercial fertilizer Control

26. Some of the “high-value” products are billion dollar industries

27. UT has developed technologies that can cost-effectively recover biofuel oils and high-value products

28. Using CEM/OpenAlgae-developed technologies, we can collaborate with other research universities and private institutions to advance health applications

29. The processed algae biomass is useful too -- we have initiated an algae fertilizer pilot program on the UT campus