1. ubio quickVet RaPId canine Parvovirus
antigen lateral Flow Immuno-assay
ubio Biotechnology systems Pvt. ltd.
Ph: +91-484-2532966, +91-9446438678
web: http://www.ubio.in email: contact@ubio.in
ubio quickVet canine Parvovirus antigen Rapid test Kit is a qualitative immunochromatographic assay
for the detection of Parvovirus cPV2 antigen in faeces in just 5-10 minutes.
PaRVoVIRus cPV2
cPV2, is a virus that attacks and destroys cells in lymph nodes, intestinal crypts and bone marrow. In-
fected dogs excrete the virus along with faeces. dogs are infected by oral contact with infected material.
the virus causes inflammation of intestines in adult dogs with occasional mortality and fatal cardiac ar-
rests in pups. Infected dogs show symptoms within 5-10 days.
the major clinical features of the disease are sudden persistant vomiting, refusal to eat or drink, lethargy,
fever, watery diaorrhea with blood, rapid dehydration and weight loss. untreated animal dies within 48-
72 hours, mainly due to dehydration, secondary infections and endotoxemia.
cPV2 infection is not transmissible to humans.
assay oVeRVIew
ubio quickVet canine Parvovirus antigen Rapid test is a lateral flow
assay designed so that even a technician with limited expertise can
perform the test easily. the veterinarian or the technician collects
a sample of faeces using the swab provided with the test. the swab
is inserted into the assay diluent vial and agitated so that the sample
is extracted into the diluent. three drops of this diluent is then
added to the sample hole on the test card using the dropper pro-
vided with the test.
the results can be read in just 5-10 minutes - the control line,
marked c, should be always appear if the test procedure is per-
formed properly and the test reagents are working. a red test line,
marked t, will be visible if canine Parvovirus cPV2 antigen is pres-
ent in faeces. Positive, Negative and
the test detects cPV2, cPV2a, cPV2b and cPV2c variants of the Invalid Results
virus.
assay comPoNeNts aNd PRINcIPle
ubio quickVet RaPId canine Parvovirus antigen lateral Flow Immuno-assay is a sandwich assay that
consists of the following components:
• sample pad
an absorbent pad onto which the test sample is applied.
• conjugate pad
this pad contains cPV2 antibody conjugated to red-coloured colloidal gold coloured parti-
cles.
doc No. RdtB000003, Revision 1.01, dtd 03 January 2011.
a printed copy of the document is uncontrolled

2. • Reaction membrane
a hydrophobic nitrocellulose membrane through which the test sample can flow.
• capture/test zone
an area on the reaction membrane onto which cPV2 antibody is immobilized in a line.
• control zone
an area on the reaction membrane onto which cPV2 antigen is immobilized in a line.
• wick or waste reservoir
an absorbent pad designed to draw the sample across the reaction membrane by capillary
action and collect it.
these components are fixed on an inert plastic backing ma-
terial mounted inside a plastic cassette with properly sized assay PaRameteRs
ports for adding the sample and for reading the result. the
sample addition port is aligned along the top of the sample
pad and the result port is aligned along the reaction mem- Sensitivity
brane showing the test and capture zones.
the sample that is added to the sample port migrates from 98.5%
the sample pad along the conjugate pad where any cPV2
antigen present in the sample will bind to the conjugated Specificity
antibody. the sample then continues to migrate across the
membrane until it reaches the capture zone where the anti- 98.0%
gen-conjugate complex will bind to the immobilised cPV2
antibody producing a visible line on the membrane. If cPV2 data based on comparison with
antigen is not present in the sample, no reaction occurs in industry-standard elIsa tests. Positive
the capture zone and no test line is formed. the sample result confirms diagnosis, but negative
then migrates further along the strip until it reaches the result does not exclude parvovirosis as
control zone, where excess antibody conjugate will bind to virus excretion in the faeces is intense
the immobilized cPV2 antigen and produce a second visi- but short. False positives may occur if
ble line on the membrane. this control line indicates that the test is performed within 12 days of
the sample has migrated across the membrane as intended. vaccination.
doc No. RdtB000003, Revision 1.01, dtd 03 January 2011.
a printed copy of the document is uncontrolled