1. CoolCell TM
Cell Freezing Container
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2. CoolCell
 An alcohol-free cell freezing
container
CoolCell
 Replaces “Mr. Frosty”
 An alcohol-filled freezing container
Mr. Frosty
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3. Background
 Most cell culture researchers freeze cells in a
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-80 C freezer prior to storing in liquid nitrogen
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 They need a slow freeze rate of -1 C/minute
 “Mr. Frosty” is commonly used now
 It uses 100% iso-propylalcohol (IPA) to control the freezing
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rate to approximately -1 C/minute
 “CoolCell” controls the freezing rate MORE
CONSISTENTLY
 with no alcohol – no fluids – no maintenance – no cost
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4. “Mr. Frosty”
 The alcohol in “Mr. Frosty” must be replaced
every 5 uses in order to keep the freeze rate
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near the desired -1 C/minute. This means…..
 you have to keep track of how many times you use it (hassle!)
 you have to purchase alcohol (expense!)
 you have to dispose used alcohol (hassle, smell & expense!)
 More importantly, it also means that the alcohol
level changes every time it is used
 “Mr. Frosty” results in different freezing profile if not maintained
properly
 It is a heat sucker “Caution” can ruin your samples/probes in
freezer
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5. CoolCell
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 CoolCell delivers a very consistent -1 C/minute
freeze rate without alcohol
 Freezing profiles are extremely consistent every time
 Less variability and high repeatability in your research
Fusion time and cooling profiles are
highly reproducible from run to run
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6. CoolCell
 CoolCell outperforms “Mr. Frosty” in cell viability
 Researchers care most about cell viability (i.e. how many cells
are still viable after they are thawed out at a later date)
In primary human cells, CoolCell significantly outperforms
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7. Additional CoolCell Advantages
 No on-going cost – COST SAVINGS!
 “Mr. Frosty” costs approx. $350/year for alcohol purchase & disposal
 Lid is easy to open – even when frozen
 “Mr. Frosty” screw cap is very difficult to open when frozen
 Not cold to the touch and not “frosty” when frozen
 “Mr. Frosty” requires special gloves to handle and is very slippery due to frost/ice
on exterior
 Ready to use again after only 5 minutes
 “Mr. Frosty” requires about 1.5 hours for alcohol to thaw before it can be used
again
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8. CoolCell vs “Mr. Frosty”
CoolCell “Mr. Frosty”
No alcohol Requires 250ml alcohol
• No hazardous fluids • Replace alcohol every 5 uses (250ml)
• Solid core and insulation design • Keep track of # of uses
• No pre-cooling required • Alcohol degrades slightly each time it’s used
• Pre-cool alcohol in refrigerator
Very consistent -1oC/min rate Inconsistent freeze rate
• All vials have uniform freeze rate every time • Each run is slightly different due to alcohol
• Radially symmetric – design enables consistency degradation
• Two circles of wells – different freeze rates for each
circle
Cost savings ~$338/year maintenance per unit
• No maintenance cost • Change alcohol weekly (~$6/wk)
• Pays for itself quickly • Dispose haz waste (~$0.75/wk)
• 10 units = $3,380 to use EVERY YEAR
Lid opens easily Screw cap very difficult to remove when frozen
• Not cold to the touch when removing from the -80C • Protective gloves required when handling frozen unit
freezer
Better cell viability Good cell viability
Ready to use again in 5 minutes Takes >1 hour for device to return to room
temp for re-use
Low impact on freezer Large thermal mass impacts local freezer area
• 1/3 the heat impact on freezer compared to alcohol- • Large heat capacity removed from alcohol impacts
filled units nearby samples
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9. CoolCell video
Click here
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10. Are you a CoolCustomer?
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11. Thank you
Michelle Nemits
VP, Sales and Business Development
Cell: 415-336-4415