Jim Anderson, Jim Kolmer, Matt Rouse, Yue Jin

1. Breeding for Rust Resistance
at the University of Minnesota
Jim Anderson
Dept. of Agronomy & Plant Genetics
University of Minnesota
St. Paul, MN
Jim Kolmer, Matt Rouse, Yue Jin
USDA-ARS
St. Paul, MN

2. U.S. Wheat Production Regions
HRS
SWW
HRW SRW

3. Wheat Breeding Priorities
Agronomic Characteristics
1. Yield 4. Shattering
2. Lodging resistance 5. Kernel color
3. Test Weight 6. Pre-harvest sprouting resistance
Diseases
1. Fusarium head blight (scab) 4. Bacterial Leaf Streak
2. Leaf Rust 5. Leaf Spotting (Tan Spot, Septoria’s)
3. Stem Rust 6. Stripe Rust
7. Barley Yellow Dwarf Virus
Bread-Making Quality Characteristics
1. % protein 4. Flour Water Absorption
2. Mixing Properties 5. Kernel Hardness
3. Loaf Volume 6. Milling Yield
7. Percent Flour Ash

4. University of Minnesota
Wheat Breeding Timeline
Year Generation No. (Loc.)
1 Crossing/F1 300
F2 (300 x 1000) 300,000
2 F3 Winter Nursery 30,000
F4 Headrows 24,000 (2)
3 F5 Scab 2,400 (2)
4 Winter Nursery 1,000
Prelim. yield trial 550 (2-3)
5-6 Adv. yield trial 170 (8-10)
7-9 MN Variety trial 10 (12)
Lr, Sr FHB Quality
Prot/TWT/Mixo
+ flour color
+ bread bake
Same as AYs

5. Use of Markers
1. Parental Characterization
2. Enrichment of BC1F1’s and 3-way crosses
3. Screen all F6’s (~1,000 lines) with 12 markers
RR
Rr
rrx
rrx
Rr rror
RR
Rr
rrx
RRx
RR Rror

6. High Priority Genes
Fusarium Head Blight (2 genes)
High Molecular Weight Glutenins (2–3 genes)
Stem Rust (esp. Ug99–effective genes)
Leaf Rust (Lr34)
Waxy
Grain Protein (GPC–6B)
Tan spot (Tsn1)
Reduced height (Rht1 and Rht2)

7. Crossing Parent Marker Database

8. Leaf Rust Mapping/Crossing Targets
• “Durable” resistance with combinations of
Lr13 + Lr16 + Lr23 + Lr34
• Ulen/Thatcher
• AC Taber/Thatcher
• RB07/Faller
• both have defeated gene Lr21, but RB07
less receptive as adult plant
• Introgressing:
• Lr52, Americano 44d (Uruguayan
landrace)

9. Backcrossing Ug99-Effective Genes
• Preliminary yield trials of “NIL” lines in 2013
• Funded by MN AES Rapid Response Project

10. “Forward” Breeding Ug99-Effective
Genes (F4 Headrows in 2013)

11. “Forward” Breeding Ug99-Effective
Genes (F4 Headrows in 2013)