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Announcements
• handouts for ch. 1 and 2 posted on ecommons
• This week’s sections meet in the greenhouse

Assignments:
• All labs need to be turned in by 2:00 on Friday
• First plant of the week is due at the beginning
of class on May 17th
• Also Quiz #4 is on Thursday
Leaf anatomy correction
Cuticle:
• Waxy layer covering the epidermis
• Impermeable to water

Cuticle and Epidermis inhibit water loss but also
inhibit CO2 diffusion
Fig. 34.11
A summary primary and secondary growth in a
               woody stem
Primary growth
• Buds protect the apical
meristem

• Only buds consist of
primary tissues

• Lengthening of shoots/
roots and production of new
roots/shoots through
branching
                               Fig. 34.16
Secondary Woody Growth: Eudicots
         Does not occur in monocots!!!

• Apical Meristem->
2 Lateral meristems

Vascular Cambium
Cork Cambium

Gymnosperms
Woody Eudicots
                      Fig 34.16
Secondary Woody Growth: Eudicots
Vascular Cambium
• 2ndary Xylem = wood;
water transport
• 2ndary Phloem= Bark;
photosynthate transport
Secondary Woody Growth
Bark

• Periderm
   • Cork
   • Cork Cambium
• Secondary Phloem
Secondary Woody Growth
Secondary Phloem
• Transport of photosynthates
from leaves to roots

Cork Cambium
• Tissue layer that produce Cork

Cork
• Outer waxy walled protective
cells
Girding blocks phloem transport
Secondary Woody Growth
Pith rays:
• Produce continuos
cylinder of vascular
cambium
Secondary Woody Growth
Products of Vascular Cambium

• 2ndary Xylem
  • Vessel slements
  • tracheids
  • Supportive Fibers

• 2ndary Phloem
  • Sieve tube elements
  • Companion cells
  • Fibers
  • Parenchyma Cells
Secondary Woody Growth
Lenticels:
• Structures on stems that allow for gas
exchange

* for metabolic: allow CO2 to go out of cell
Shoot System
Consist of leaves,
stems, and flowers

Stems and Branches
• Support leaves
• Allow transport of
materials between
roots and leaves
Shoot System

Phytomer
 • 1 or more leaves
attached at the node; an
internode, and 1 or more
axillary buds

Axillary Bud
 • If active develops into
a new branch
Shoot System
Leaf
• Consist of a blade and a petiole.
   • Petiole attaches leaves to
     stems

Node: Point of leaf attached
Phyllotaxy (Leaf Arrangement):
  How many leaves are attached at the node?

alternate
1 leaf per node

opposite
2 leaves per node

Whorled
3+ leaves per node
Root System
• Eudicots
  - Tap root
     • Often functions as carbohydrates storage organ
      •Ex. Carrots, Sugar Beet

- Later roots:
  • Roots that extend outward form the tap root
Root System
• Monocots
  - Tap root
    • Short lived


 - Adventitious Roots
    • Arise from the stem at ground level or below
    • Form fibrous root system


  - Prop Roots
    • Help Support the shoot
Roots: Overall Function
• Anchorage and support of stems
• Carbohydrate storage
•Water and nutrient uptake
Biology 20B
     CHAPTER 35

Water and Solute Transport
Transport in Plants
3 important modes of transport in plants

• Passive transport
       • Diffusion of molecules across a membrane from a high to low
         concentration gradient
            - Ex. Osmosis

• Active transport
    - The use of energy in the form of ATP to move ions against their
      concentration gradient.
          - Ex. Proton pump

• Facilitated trasnport
    - Changes in the electron gradient within cells allows for diffusion
      of ions via specific membrane channels
           - Ex. K + movement
WATER AND SUGAR TRANSPORT
First, need to understand the passive movement of water:

• Water potential - tendency of water to move
across a membrane = ψ
• Osmosis- Passive diffusion of water from a
region with higher ψ to one of lower (more
negative) ψ

                      ψ        =        ψs +               ψp
       Water pot. = solute pot.+ pressure pot.

                     Generate osmotic pressure    Physical pressure water is under
WATER AND SUGAR POTENTIAL
Osmosis = Passive diffusion of water from a
region with higher ψ to one of lower (more
negative) ψ .

ψs= usually negative
ψp= usually positive

Water always moves across a selectively
permeable membrane towards the region of
lower (more negative) water potential
Water Potential and Water Transport
ψs of a salt solution is LOWER than ψs of distilled water.



ψs of 0.1M solution = -0.23 Mpa
Mpa = “megapascals” atmospheric pressure = 0.1 Mpa

ψs of distilled water = 0.00 Mpa
Ex. Leeches
Salt on leeches changes the osmotic potential.
water leaves their bodies and they fall off
In an animal cell, “kerplow” it bursts

But in a plant cell.....
when higher ψ surround->      Turgor pressure~ 0.7 Mpa
Wall pressure = turgor pressure



                     “Turgid cell”
Turgor Pressure
• Turgor Pressure- pressure that is exerted
against the cell wall as a result of water
entering the cell
Plasmolysis
When lower ψ surrounds
(e.g. concentrated solution or dry air)

PLASMOLYSIS
= cells are “flaccid”
(=BAD)--> dehydration
Water in Plants
• Wilting and Rehydration (turgor pressure)



PLANT VIDEO PLACE HERE*
How does water move through the plants

Short -distance movement (within tissues)
(e.g. outer roots cells---> inner cells)

1. APOPLASTIC
    • Between cells and cell walls

2. SYMPLASTIC
   • Through cells (cytoplasm), cell to cell
Casparian strip
                                                   • Within endodermis
       The Casparian strip prevents water and      • Surrounds plumbing
       ions in the apoplast from passing between
       the endodermal cells into the stele.        • Stops apoplastic flow
an
                                                   • Solutes & water can
ess
ast.
                                    Endodermis
                                                   only enter through cell
                                                   membrane
                               Pericycle           • Sophisticated pumps
                                                   Toxics out
                                                   Selective ion uptake
                                       Casparian
                                                   • Pericycle
Transpiration
Leaves can loose up to 100% of their water in 1
hour

Plants retain less than 1% of the water they
absorb

no need to copy this*
Water is a Strange Molecule
• Polar molecule
• Adhesion - Sticks to other molecule
• Cohesion - water sticks to itself
Water Potential
• Differences in water            Low water potential
potential determines              Atmosphere ψ: -95.2 MPa
the direction that                (Changes with humidityl
water moves                       usually very low)


                                  Leaf ψ: -0.8 MPa
• Water always flows
                                  (Depends on transpiration
from high to low water            ratel low when stomata are
potential                         open_


• Water potential                 Root ψ: -0.6 MPa
gradient exist between            (Medium -high)
the soil, plants, and the
atmosphere
How does water move through the plant?

Now we are in the leaves.
  What generates the force that pulls the
  water column through the shoot?




                               • The plants needs carbon
                               • The plant need to stay hydrated.
                               (A plant may lose 300 g of water
                                for every 1g of glucose produced)
How do stomata regulate water loss?
• 2 guard cells.
Flaccid: stoma closed
Turgid: stoma open

• Day: open & night: closed
Mechanism?
• Blue right receptor --->
• Stimulates proton pump...
How guard cells work: the proton pump (review)


1. H+ moves out against
elctronchem grandient
Use ATP energy

2. K+ and CL- moves in through
ion channel in response to -ψ

3. Water moves in by osmosis
because [ion] is high
Stomata close when:
Dark
High CO2
Water stress
High temp
Sugars: Translocation
• How do sugars made in leaves end up in other
parts of the plant?
Sugars are transported in the phloem



           Aphid and Ants
Now, on to movement of sugars..
Translocation = phloem transport
    • Energetically demanding process.

    Sieve tube members (+companion cells)
    + Minerals, amino acids, hormones....

    Materials can go either up or down
    SOURCE vs. SINK
    (but only one direction per tube)
bio20b 512  34-35
bio20b 512  34-35
bio20b 512  34-35
bio20b 512  34-35

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bio20b 512 34-35

  • 1. Announcements • handouts for ch. 1 and 2 posted on ecommons • This week’s sections meet in the greenhouse Assignments: • All labs need to be turned in by 2:00 on Friday • First plant of the week is due at the beginning of class on May 17th • Also Quiz #4 is on Thursday
  • 2. Leaf anatomy correction Cuticle: • Waxy layer covering the epidermis • Impermeable to water Cuticle and Epidermis inhibit water loss but also inhibit CO2 diffusion
  • 4. A summary primary and secondary growth in a woody stem
  • 5. Primary growth • Buds protect the apical meristem • Only buds consist of primary tissues • Lengthening of shoots/ roots and production of new roots/shoots through branching Fig. 34.16
  • 6. Secondary Woody Growth: Eudicots Does not occur in monocots!!! • Apical Meristem-> 2 Lateral meristems Vascular Cambium Cork Cambium Gymnosperms Woody Eudicots Fig 34.16
  • 7. Secondary Woody Growth: Eudicots Vascular Cambium • 2ndary Xylem = wood; water transport • 2ndary Phloem= Bark; photosynthate transport
  • 8. Secondary Woody Growth Bark • Periderm • Cork • Cork Cambium • Secondary Phloem
  • 9. Secondary Woody Growth Secondary Phloem • Transport of photosynthates from leaves to roots Cork Cambium • Tissue layer that produce Cork Cork • Outer waxy walled protective cells
  • 11. Secondary Woody Growth Pith rays: • Produce continuos cylinder of vascular cambium
  • 12. Secondary Woody Growth Products of Vascular Cambium • 2ndary Xylem • Vessel slements • tracheids • Supportive Fibers • 2ndary Phloem • Sieve tube elements • Companion cells • Fibers • Parenchyma Cells
  • 13. Secondary Woody Growth Lenticels: • Structures on stems that allow for gas exchange * for metabolic: allow CO2 to go out of cell
  • 14. Shoot System Consist of leaves, stems, and flowers Stems and Branches • Support leaves • Allow transport of materials between roots and leaves
  • 15. Shoot System Phytomer • 1 or more leaves attached at the node; an internode, and 1 or more axillary buds Axillary Bud • If active develops into a new branch
  • 16. Shoot System Leaf • Consist of a blade and a petiole. • Petiole attaches leaves to stems Node: Point of leaf attached
  • 17. Phyllotaxy (Leaf Arrangement): How many leaves are attached at the node? alternate 1 leaf per node opposite 2 leaves per node Whorled 3+ leaves per node
  • 18. Root System • Eudicots - Tap root • Often functions as carbohydrates storage organ •Ex. Carrots, Sugar Beet - Later roots: • Roots that extend outward form the tap root
  • 19. Root System • Monocots - Tap root • Short lived - Adventitious Roots • Arise from the stem at ground level or below • Form fibrous root system - Prop Roots • Help Support the shoot
  • 20. Roots: Overall Function • Anchorage and support of stems • Carbohydrate storage •Water and nutrient uptake
  • 21. Biology 20B CHAPTER 35 Water and Solute Transport
  • 22. Transport in Plants 3 important modes of transport in plants • Passive transport • Diffusion of molecules across a membrane from a high to low concentration gradient - Ex. Osmosis • Active transport - The use of energy in the form of ATP to move ions against their concentration gradient. - Ex. Proton pump • Facilitated trasnport - Changes in the electron gradient within cells allows for diffusion of ions via specific membrane channels - Ex. K + movement
  • 23. WATER AND SUGAR TRANSPORT First, need to understand the passive movement of water: • Water potential - tendency of water to move across a membrane = ψ • Osmosis- Passive diffusion of water from a region with higher ψ to one of lower (more negative) ψ ψ = ψs + ψp Water pot. = solute pot.+ pressure pot. Generate osmotic pressure Physical pressure water is under
  • 24. WATER AND SUGAR POTENTIAL Osmosis = Passive diffusion of water from a region with higher ψ to one of lower (more negative) ψ . ψs= usually negative ψp= usually positive Water always moves across a selectively permeable membrane towards the region of lower (more negative) water potential
  • 25. Water Potential and Water Transport
  • 26. ψs of a salt solution is LOWER than ψs of distilled water. ψs of 0.1M solution = -0.23 Mpa Mpa = “megapascals” atmospheric pressure = 0.1 Mpa ψs of distilled water = 0.00 Mpa
  • 27. Ex. Leeches Salt on leeches changes the osmotic potential. water leaves their bodies and they fall off
  • 28. In an animal cell, “kerplow” it bursts But in a plant cell..... when higher ψ surround-> Turgor pressure~ 0.7 Mpa Wall pressure = turgor pressure “Turgid cell”
  • 29. Turgor Pressure • Turgor Pressure- pressure that is exerted against the cell wall as a result of water entering the cell
  • 30. Plasmolysis When lower ψ surrounds (e.g. concentrated solution or dry air) PLASMOLYSIS = cells are “flaccid” (=BAD)--> dehydration
  • 31. Water in Plants • Wilting and Rehydration (turgor pressure) PLANT VIDEO PLACE HERE*
  • 32. How does water move through the plants Short -distance movement (within tissues) (e.g. outer roots cells---> inner cells) 1. APOPLASTIC • Between cells and cell walls 2. SYMPLASTIC • Through cells (cytoplasm), cell to cell
  • 33.
  • 34. Casparian strip • Within endodermis The Casparian strip prevents water and • Surrounds plumbing ions in the apoplast from passing between the endodermal cells into the stele. • Stops apoplastic flow an • Solutes & water can ess ast. Endodermis only enter through cell membrane Pericycle • Sophisticated pumps Toxics out Selective ion uptake Casparian • Pericycle
  • 35. Transpiration Leaves can loose up to 100% of their water in 1 hour Plants retain less than 1% of the water they absorb no need to copy this*
  • 36. Water is a Strange Molecule • Polar molecule • Adhesion - Sticks to other molecule • Cohesion - water sticks to itself
  • 37. Water Potential • Differences in water Low water potential potential determines Atmosphere ψ: -95.2 MPa the direction that (Changes with humidityl water moves usually very low) Leaf ψ: -0.8 MPa • Water always flows (Depends on transpiration from high to low water ratel low when stomata are potential open_ • Water potential Root ψ: -0.6 MPa gradient exist between (Medium -high) the soil, plants, and the atmosphere
  • 38. How does water move through the plant? Now we are in the leaves. What generates the force that pulls the water column through the shoot? • The plants needs carbon • The plant need to stay hydrated. (A plant may lose 300 g of water for every 1g of glucose produced)
  • 39. How do stomata regulate water loss? • 2 guard cells. Flaccid: stoma closed Turgid: stoma open • Day: open & night: closed Mechanism? • Blue right receptor ---> • Stimulates proton pump...
  • 40. How guard cells work: the proton pump (review) 1. H+ moves out against elctronchem grandient Use ATP energy 2. K+ and CL- moves in through ion channel in response to -ψ 3. Water moves in by osmosis because [ion] is high
  • 41. Stomata close when: Dark High CO2 Water stress High temp
  • 42. Sugars: Translocation • How do sugars made in leaves end up in other parts of the plant?
  • 43. Sugars are transported in the phloem Aphid and Ants
  • 44. Now, on to movement of sugars.. Translocation = phloem transport • Energetically demanding process. Sieve tube members (+companion cells) + Minerals, amino acids, hormones.... Materials can go either up or down SOURCE vs. SINK (but only one direction per tube)

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