description about pseudomonas bacteria

2. Scientific classification
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas
Species: P. aeruginosa
Binomial name
Pseudomonas aeruginosa

3. Pseudomonas Infections
Survive where most
organisms cannot;
e.g., “oil-eating”
bacteria are
Pseudomonas.
(Slime layer)
Opportunistic pathogen
Flagella

4. Pseudomonas aeruginosa

5. Structure

6. 1.Exotoxin A
 Similar in structure to Diptheria toxin
 Causes Dermatonecrosis in burn
wounds, corneal damage in ocular
infections, and tissue damage in
chronic pulmonary infections.
 Also this toxin is immunosuppressive
Heat labile hemolysin
Breaks down lipids and lecithin causing
tissue destruction
Stimulates inflammatory response
2.Phospholipase C

7. 3.Elastase and Alkaline Protease
• Destruction of elastin-containing tissues
(blood vessels, lung tissue, skin), collagen,
immunoglobulins, and complement factors
• Can produce hemorrhagic lesions (ecthyma
gangrenosum)
• Inactivation of interferon

8. Clinical Disease
Pulmonary Infections
Burn Wound Infections and other skin and soft tissue
infections (life threatening)
UTI’s (especially catheterized)
External Otitis (malignant OE, swimmer’s ear)
Eye Infections and corneal ulceration via contaminated
contact lens cleaning fluids
Pseudomonal Endocarditis

10. Grow on most laboratory media
Blood agar
MacConkey’s agar
Minimal nutritional reqts.;
Many organic compounds used
as C and N sources, but only a
few carbohydrates by oxidative
metabolism
tolerate broad temperature
spectrum
its ability to grow at 42° C.
Isolation and identification

11. Motility of organism

12. Colony characteristic:
 Characteristic grape-like odor and blue-green pus & colon
 Lactose negative on MacConkey’s agar
 Some strains produce diffusible pigments:
Pyocyanin (blue);
Fluorescein (yellow);
Pyorubin (red)
 Fluorescence is helpful in early identification
CharacteristicsCharacteristics
Biochemical test:
inability to ferment lactose,
oxidase & catalase positive
Nitrate reduction negative
Catalase test

13. THANK YOU