1. Metal-Free PEEK columns for
HPLC・LC/MS
Metal-Free PEEK columns for HPLC and LC/MS
Analytes containing phosphate groups creates the formation of phosphate-iron complexes, which often lead
to deteriorated peak shape or poor precision of quantitative analysis. In the past, the residual metallic
impurities in the packing material were assumed to be the cause of such problem. However, the influence
of the column material also provide a big impact to such trace analytes in high sensitivity analysis.
InertSustain and Inertsil series HPLC columns are now available in PEEK hardwares to deliver excellent peak
shapes for various analytes without any adsorption. The chromatographic performance of these columns is
identical to their metal counterparts with the added advantage of simply better chemical Inertness.
2. ・Material : Wetted Part … PEEK
Outer Part … Stainless Steel
・Particle Sizes :1.9 μm, 2 μm, 3 μm
・Packing Materials : InertSustain, Inertsil series
・Max. Operating Pressure :80 MPa(1.9 μm、2 μm)、 50 MPa(3 μm)
■ UHPLC-PEEK Columns
■ Standard PEEK Columns
Packing Material
PEEK
PEEK columns are now available in sub-2 μm to 10 μm particle sizes
Generally, conventional PEEK hardware is tolerable up to a back
pressure of 20 MPa. The mechanical strength of PEEK is far less than
stainless steel and the durability to be very low as well. As illustrated
on the right, the introduction of a radically new Steel-Coated-PEEK
hardware offer maximum operating pressure of 80 MPa for sub-2 μm
columns achieving rapid analysis for analytes sensitive to frequently
used stainless steel columns.
Not only the hardware, but the frits are also made from PEEK, which
the sample contacts only PEEK.
Specification
・Material :PEEK
・Particle Sizes :4 μm, 5 μm, 10 μm
・Packing Materials : InertSustain, Inertsil series
・Max. Operating Pressure :20 MPa (200 Bar)
Specification
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 min
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000 1:768.05>261.10(+) CE: -31.0
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 min
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000 1:768.05>261.10(+) CE: -31.0
: InertSustainSwift C18 (1.9 μm, 50 x 2.1 mm I.D)
: A) 5 mM Ammonium formate in H2O
B) 5 mM Ammonium formate in CH3CN
A/B=98/2-(2 min)-80/20-(0.01 min)-98/2-(2.99 min)-98/2
: 0.4 mL/min
: 40 ℃
: LC/MS/MS (ESI)
: 10 μL
: Coenzyme A
Conditions
Column
Eluent
Flow Rate
Col. Temp.
Detection
Injection Vol.
Sample
UHPLC-PEEK Stainless Steel
SH
NH NH
O O
OH
CH3
CH3
O
O
OHO
P
O
OH
O
OH
O
O
P
P
OH
OH O
N
N
N
N
NH2
■ Comparison of Performance
0 1.0 2.0 3.0 4.0 5.0 6.0 7.0
Time(mm)
0 1.0 2.0 3.0 4.0 5.0 6.0 7.0
Time(mm)
Stainless Steel
PEEK Packing Material
3. ■ Ordering Guide
Metal-Free PEEK columns for HPLC and LC/MS
All InertSustain & Inertsil series phases are now available in PEEK hardwares upon request.
When ordering please refer to the following complete ordering guide.
As all of these PEEK columns are custom-made products, use catalog number "5020-" only.
GL Sciences can pack virtually any packing material you request into a PEEK column format.
For other packing material, please inquire.
* As shown below, end-fittings are “UP” type end-fittings, which are UHPLC compatible end-fittings for
UHPLC systems (Ex: UPLC ®) to avoid dead volume.
UPLC ® is a registered trademark of Waters Corporation.
2.4 mm
10-32UNF
12. Metal-Free PEEK columns for HPLC and LC/MS
Worldwide Ordering Information
GL Sciences, Inc. USA
4733 Torrance Blvd. Suite 255
Torrance, CA 90503
Phone: 310-265-4424
Fax: 310-265-4425
Email: info@glsciencesinc.com
Web: www.glsciencesinc.com
GL Sciences, Inc. Japan
22-1 Nishishinjuku 6-Chome
Shinjuku-ku, Tokyo,
163-1130, Japan
Phone: +81-3-5323-6620
Fax: +81-3-5323-6621
Email: world@gls.co.jp
Web: www.glsciences.com
International Distributors
Visit our Website at
www.glsciences.com/distributors
GL Sciences B.V.
De Sleutel 9
5652 AS Eindhoven
The Netherlands
Phone: +31 (0)40 254 95 31
Email: info@glsciences.eu
Web: www.glsciences.eu
• The GL Sciences name, the GL Sciences logo are the property of GL Sciences Inc.
• All other trademarks or service marks are the property of their respective owners.
• The specification and the column type are subject to change without notice due to continual improvements.
AA851-20140901PDF
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