1. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
1

2. CONTENTS:
The components of the high performance liquid
chromatograph (HPLC).
The separation process.
The chromatogram.
2

3. Component:
Solvent
Pumps
Column
Detector
Chromatogram
3

4. 4
Separation in based upon differential
migration between the stationary and
mobile phases.
Stationary Phase - the phase which
remains fixed in the column, e.g. C18,
Silica
Mobile Phase - carries the sample
through the stationary phase as it
moves through the column.
Injector
Detector
Column
Solvents
Mixer
Pumps
High Performance Liquid Chromatograph
Waste

5. 5
Injector
Detector
Column
Solvents
Mixer
Pumps
Chromatogram
Start Injection
mAU
time
High Performance Liquid Chromatograph

6. 6
Injector
Detector
Column
Solvents
Mixer
Pumps
Chromatogram
Start Injection
mAU
time

7. 7
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

8. 8
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

9. 9
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

10. 10
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

11. 11
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

12. 12
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

13. 13
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

14. 14
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

15. 15
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

16. 16
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

17. 17
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

18. 18
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

19. 19
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time

20. 20
Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time