I made this presentation to show Kelly about my ideas regarding how I could apply Optical Tweezers with unzipping telomeric DNA

1. Telomeres and Unzipping
Mapping the path to the Fountain of
Youth
By:

2. Technique
Using techniques from Shotgun Chromatin/DNA Mapping (SCM/SDM)
we hope to map a variety of useful DNA structures/components. One
such application could be to map Telomeres.

3. 3’ Overhang
G-rich strand
F
Telomere
DNA
F
How to unzip?
Using techniques established by others, it is possible to isolate a telomere
from other chromosomal DNA using cutting enzymes. We can attach the
telomere fragment to Koch’s unzipping scheme like any other DNA/chromatin
fragment.

4. A B
C
Possible Unzipping Scenarios
A. Naked Telomere DNA
B. T-loop with proteins (TRF1 and TRF2 shown)
C. G-quadruplex structures on Naked Telomere DNA
D. (Unshown) Some combination of the above.