How to Get Started in Social Media for Art League City
Presentationrise amib
1. Isolation, Purification and
Characterization of Gêmeos and
Isinaayusferti, bacteriophages
Isolated from Tropical Soils
Amanda M. Icazatti Burtell, Fernando Pachecho Ocasio,
John Rivera Meléndez & Leyda Villagrasa Méndez
Dr. Michael Rubin
RISE Program
University of Puerto Rico at Cayey
2. Introduction
• What is a bacteriophage?
• What host bacteria are being used?
– Mycobacterium smegmatis
– Bacillus cereus
• Where can bacteriophages be found?
• What do bacteriophages need for living?
• Types of life cycles: Lytic vs. Lysogenic cycle
3. • Why are bacteriophages important?
Phages can be used in various fields of science such
as:
- Genetics - Cloning and mutation of DNA and
studies in genome
- Therapeutic uses for the creation of antibiotics
resistant to bacteria
• What is the purpose of this experiment?
4. Problem and Hypothesis
• Problem: Can novel and interesting
bacteriophages be isolated from the tropical
soils of Puerto Rico?
• Hypothesis: Both Mycobacteriphages and
Bacilophages will be found on the tropical
soils of Puerto Rico.
10. Gêmeos (bacilophage)
• Coordenates: Lat: 23' 36" 23 S Long: 46' 41" 51 W
• Temperature: 19˚C
• Details: Dark and damp soil
• Found in a populated area near a river
• Sao Paolo. Brazil
12. Sample Coordenates Details Depth Temperature Date Place
Sample
#1
LVM
Lat: 18.2333419
Long:
-66.0478104
Humid and
plain soil
2” 26˚C Feb/3/14 Caguas,
PR
Sample
# 2
LVM
LAT: 18.1192448
LONG:
-66.1667764
Dry and
lumpy soil
1” 25˚C Feb/16/
14
Cayey,
PR
Sample
#3
LVM
Lat: 18.2333419
Long:
-66.0478104
Humid and
plain soil
3” 27˚C Feb/23/
14
Caguas,
PR
13. Sample Coordenates Details Depth Temperatur
e
Date Place
Sample
#1
JLR
Lat: 18 Nᵒ
Long: 66 Wᵒ
Humid and
saturated
soil
1‘’ 31˚C Feb/3/14 Cidra,
PR
Sample
# 2
JLR
Lat: 18 Nᵒ
Long: 66 Wᵒ
Dry soil
under
trash
disposal
Surface
sample
32˚C Feb/21/14 Cidra,
PR
14. Isinaayusferti (mycobacteriophage)
Coordenates: Lat: 18˚ 14’ .0312 " N
Long: 66˚ 2' 52.116" W
Temperature: 27 ˚ C
Place: Caguas, Puerto Rico
Details: Humid and plain soil. Found near the
garbage in front of a house.
17. Conclusion
• The soil samples were collected and isolated
using the enrichment method.
• A bacillophage was found in Sao Paolo, Brazil
and was named Gêmeos.
• Third Purification Stock Filtrate (Gêmeos)
• A mycobacteriophage found in Caguas, Puerto
Rico was named Isinaayusferti.
• Concentrated Stock Phage (Isinaayusferti)