1. Selective inhibition of Leishmania donovani by active extracts of
wild mushrooms used by the tribal population of India: An in vitro
exploration for new leads against parasitic protozoans
Suvadip Mallick, Aritri Dutta, Somaditya Dey, Joydip Ghosh, Debarati
Mukherjee, Sirin Salma Sultana, Supratim Mandal, Soumitra Paloi, Somanjana
Khatua, Krishnendu Acharya, Chiranjib Pal.
Sofía Medina
Ana María Parada
III Semestre

2. INTRODUCTION

3. LEISHMANIASIS

4. L. DONOVANI
.

5. MUSHROOMS

6. EXTRACT
Is a maceration type, in which the
liquid solvent is a mixture of ethyl
alcohol and water, which dissolved
the active substances contained in
the raw material. In other words, it
is "the power of a raw material" to
become a "drug”.

7. Lab
tests
Lab
tests

8. GENERAL OBJECTIVE

10. MATERIALES Y METODOS
Preparación de
extractos

11. MATERIALES Y METODOS

12. MATERIALES Y METODOS

13. MATERIALES Y METODOS

14.  Reacción antígeno-anticuerpo basada en la identificación del
receptor de membrana por medio de fluorocromo.

Para evaluar apoptosis y variabilidad

Diferentes marcadores
MATERIALES Y METODOS

15. MATERIALES Y METODOS

16. MATERIALES Y METODOS

18. ACTIVIDAD ANTI-PROMASTIGOTE

19. ACTIVIDAD ANTI-PROMASTIGOTE

20. ACUMULACIÓN DE LÍPIDOS EN
PROMASTIGOTES

21. ACUMULACIÓN DE LÍPIDOS EN
PROMASTIGOTES

22. APOPTOSIS EN
PROMASTIGOTES

23. CONDENSACIÓN DE LA
CROMATINA Y
FRAGMENTACIÓN
NUCLEAR

24. TOXICIDAD DE LOS
EXTRACTOS ACTIVOS DE
LOS HONGOS

25. LIBERACION DE IL-12

27. Author What the author said Yes/No
Ghosh et al IL-12 interacts with NK cells
and induces IFN-g specific Th1
cells differentiation, required
for disease demolition Yes
Lai et al To emphasize the findings, a
novel astrakurkurone
molecule with excellent
antileihmanial activity against
L. donovani promastigotes has
alredy been insolated from A.
hygrometricus by our group
Yes

28. Author What the author said Yes/No
Owens In experimental visceral leishmaniasis, splenic
conventional DCs are the first cells to produce
IL-12 within the splenic microenviroment, and
are activated for heightened expression of a
variety of costimulatory molecules through both
direct interactions with Leishmania parasites
and through inflammatory signals
No mentioned
Liew et al Release of nitric oxide is associated with pro-
inflammatory cytokine TNF-a, wich has been
also reportedto enhance the killing of
intracellular amastigotes Yes

30. It´s important to not underestimate the beliefs or rituals of
tribes because as we saw in this study, it is in the beliefs
and rituals that we can start to develop new cures to
diseases whos current treatment is not accesible to
everyone
This study proved the influence of mushrooms extract on
the metabolism of lipids and the induction of apoptosis.
This can help us in finding or producing new drugs with
similar mechanism in order to efectively attack the
parasite.

31. As science has evolved, the importance of the relationship
and integration between medicine, technology and natural
resources has become more evident.
It´s risky to affirm that the addition of mushroom
extracts does not produce negative effects on the
organism because the difference in the percentage of
control and treated cellular death is not significant.

32. Ana María Parada P

33. Sofía
Medina