This is a question about cell culture. From my data, I found that th.pdf

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This is a question about cell culture. From my data, I found that the cell culture that using 2% FBS (fetal bovine serum) has a faster growth rate than the cell culture using 10% FBS. I would like to ask why does it happen? What is the possible reasons to explain this phenomenon? Also, why the amount of protein decrease when the cell number increase exponentially during cell culture?.

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Todas las siguientes afirmaciones describen correctamente alguna parte de la seleccin natural, excepto a. variacin en un elemento hereditario que se requiere para que tenga lugar la seleccin natural b. por lo general, se producen ms descendientes que pueden sobrevivir C. los rasgos que mejoran la supervivencia y la reproduccin se vuelven ms frecuentes en las generaciones futuras d. La aptitud de un organismo es directamente equivalente a su capacidad de supervivencia..

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Todas las siguientes afirmaciones describen con precisin una comunicacin positiva y saludable, EXCEPTO a. La enfermedad comunicativa puede conducir a la muerte prematura. b. La comunicacin sincera requiere integridad personal y carcter tico. C. El presidente Ronald Reagan habl al pueblo estadounidense utilizando sus valores fundamentales y aspiraciones sinceras. d. Una desventaja de la comunicacin positiva y saludable es que excluye la competencia en el lugar de trabajo..

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To what extent do you believe that social media is or is not ubiquitous for your targeted market? Not everyone participates in social media and some key demographic segments tend to avoid it for reason of privacy and security and others due to lack of knowledge and skills. To what extent for your business vision are social media and e-Business technologies appropriate tools for understanding the current market and its trends? Be sure to consider those likely to be future customers after you have successfully launched and become established and the impact of current trends..

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This data shows the relation between Tucson and Phoenix's airports and how many birds they've hit by species. The table shows which airport hit a higher percentage of a species. There is also a chi test on the data. variable PHOENIX SKY HARBOR INTL ARPT TUCSON INTL Pearson's chi-squared test data: matrixbirbs X-squared =259.84,df=19,p-value <0.00000000000000022 QUESTION 10: Are species and airport related in this dataset? Use your p value to support your answer..

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To examine the potential effects on the transmission of dengue, Bian et al. (2010) infected mosquitoes from both the WB1 (laboratory created) strain and the original wild strain with dengue. Fourteen days later, the mosquitoes were allowed to feed on an artificial food solution for 90 minute. Viral titers in the food solution were then measured (in plague feeding units [pfu] per ml. The results are given below. Mosquitoes were tested in groups, and so each data value is an average for the group, measured in pfu/ml. The data have already been log-transformed using log(Y+1), to better visualize the differences. Rank the data and use the Mann-Whitney U-test to solve for U1. Round your answer to 1 decimal placeWB1:Links to an external site.Wild: Links to an external site.811.35.910.84.49.44.46.52.46.305.904.704.2.

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To create a 2D graphics scene using the HTML canvas element. The idea and details of this scene are left up to you but must consist of different colors and2D shapes. You must make sure you to meet the following requirement: Define JavaScript variables and constants (using let and const) Use more than one conditional structure (if/else; ...) Use more than one loop (for, while, do/while) Define and call functions Make your scene interactive by having at least two components controlled using a form.

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To complete this discussion, please base your work on Enron, WorldCom, or another white collar crime story of your choosing. 1. Name the company you selected and briefly summarize the crime. 2. Do you think that the CEOs and other corporate officers involved were justly held criminally responsible or not? Explain your perspective. 3. Do you believe that business can regulate itself to act ethically, or is government oversight a necessity to protect the public from financial wrongdoing? Explain your position..

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Title: Is it a Planned Show Mrs. Jancy works with TBL global services, IT project management company, in the HR department. Jancy is known for her involvement not only in her duties but also in other various activities of the department. She was aware of many minute details about the department throughout her tenure. Hence she becomes a point of contact for any such information which often consumes her time. She is loaded with responsibilities very often, sometimes given to her and sometimes taken by her. In the beginning, she was happy with her profile and gave her the best in all the responsibilities with her. When years pass and she gets elevated to higher responsibilities she becomes overloaded. Still, she was happy with her job. One fine day suddenly, she realized that many of her colleagues complete their work on time and also equally take care of their personal life. On the other hand, Jancy, having been loaded with responsibilities completed it partially at work and partially during her personal time. This becomes a reason for her stress. She started questioning her ability to not being able to complete her work at the scheduled time. Later, she understood that apart from given responsibility she was involved in many other crucial activities of the department which becomes her additional responsibility which slowly the people forget that it is not under her job profile. Jancy Being highly disturbed started feeling her job as a burden. She thought she cant come out of this because the habit which she developed for herself and her coworkers is irreversible. So she finally decided to take a break from her job. She fixed an appointment with the head HR, Mr. Ratan, for her resignation, He knows the fact that her involvement is irreplaceable and he also knew that she can be his competitor in a very short period, so, he had some other plans for her. During the conversation, Ratan understood that Jancy is highly stubborn in leaving the job. So he convinced her to do work from home and advised her to limit her responsibilities as she will not come in any direct contact with the employees. Jancy happily accepted the option and she felt relaxed. At the same time, Mr. Ratan was reporting to CEO Mr. Vedant Sir, I didnt accept the resignation. I have given Jancy the option of work from home and she accepted to continue the job. Mr. Vedant asked but Ratan how did you made her accept this offer. And why you have given this option. As per her colleagues, her nature of doing the job is a key component. Mr. Ratan with a smile replied. Sir I advised her to limit her responsibilities to her profile by opting for work from home. She accepted the offer. And I am very sure that her very nature of involvement will not allow her to restrict her responsibility even after opting for work from home. So I am not going to lose the benefit of her involvement. Mr. Vedant was worried by the last words of Ratan, he thought.How I am going to execute my plan of replacing.

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This assignment is using Logism to create circuits. I have been trying to get this assignment done for a while for I am behind because of other coursework. If you answer the question, please make sure to read all the small details very clearly, as one small error will cause the assignment to be incorrect. I will have my own solution to the problem and will check with yours! Thank you for your help in advance. Description - This assignment aims at becoming familiar with Logisim and how to use basic circuit schematic elements. The goal of this assignment is to create a small and simple circuit that will convert three 4-bit binary numbers to a hexadecimal value each. Tasks - This assignment will have two circuits: A main circuit that will include 3 hex digit displays and inputs that allow the user to input a total of 12 bits. The second circuit is a so called subcircuit, which will be used in the main circuit and will connect the inputs to the hex displays. This subcircuit will route each bit from the inputs to the correct hex display using splitters. In Logisim, create a new file, add a subcircuit called "Converter", open it and do the following: To control the first hex digit display: Add 4 inputs with a bit width of 1 Add 1 output with a bit width of 4 Add a splitter with 4 fan outs and a bit width of 4 Connect the four 1-bit circuit inputs to the splitter fan out For each of the four inputs: Set the "Label" property to the letter "i" and append the number of the splitter fan out it is connected to (should be a number between 0 and 3). Connect the splitter output to the 4-bit output of the circuit Add the label "hex 1" to the output To control the second hex digit display: Add 2 inputs one with a bit width of 1 and the other with a bit width of 3 Add 1 output with a bit width of 4 Add a splitter with 2 fan outs and a bit width of 4 In order to make the splitter take in a group of 3 bits on one fan out and 1 bit on the second, we need to change the properties: Make sure the splitter has one fan out for bits 0, 1 and 2 (3-bit bus) and bit 3 on a separate fan out (1-bit wire). Connect the two inputs (1 bit input and 3 bit input) to the splitter fan out Connect the splitter output to the second 4-bit output of the circuit For each of the two inputs: Set the "Label" property to the letter "j" and append the number of the splitter fan out it is connected to (should be the two numbers 3 and 2,1,0, make sure the label reflects the bit position correctly) Add the label "hex 2" to the output To control the third hex digit display: Add 2 inputs with a bit width of 2 Add 1 output with a bit width of 4 Add a splitter with 2 fan outs and a bit width of 4 Connect the two 2-bit circuit inputs to the splitter fan out Connect the splitter output to the third 4-bit output of the circuit For each of the two inputs: Set the "Label" property to the letter "k" and append the bit numbers of the splitter fan out it is connected to (should be the two numbers 3,2 and 1.

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Three students, Habib, Vikki and Claire, in Bio 200 ran their samples of HindIII cut plasmid DNA side by side on the same gel and got the results shown below. Habibs bands are not as bright as Claires and Vikki has no DNA. Their lab instructor has asked them to consider where in the mini-prep procedure Habib and Vikki may have gone wrong. From the list of mistakes below, choose one mistake that could account for Habibs results and one mistake that could account for Vikkis results. Explain why they would lead to less bright bands for Habib and no DNA for Vikki. Using 1.5 ml of E.coli instead of 3 ml Not adding lysis buffer Washing with PE first and then PB Not adding enough HIndIII Not adding enough SyberSafe Vortexing their tubes when they werent supposed to Transferring the white pellet to the filter cartridge Habib Carly Vikki.

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This group assignment provides an opportunity to develop your database design skills through a hands-on activity. The focus of the assignment is to extend an existing database design at the logical level. Two scenarios are presented below, which discuss functionality that should be added to an existing design. One scenario is for the movies database and the other is for the beer database. Select only one scenario and do the following three tasks. 1.Update the design to fulfill the requirements of the selected scenario. This should include developing an extended ERD. 2. List the critical assumptions you made in your design. Also, discuss how those assumptions may limit current functionality. The current movie database design lacks the functionality to support theaters and show times. It is not possible to see what theaters a movie is currently playing in or what movies are being shown at a particular theater. Therefore, we would like to extend the design to capture the following information. a.Different movie theaters. The design should allow data to be stored such as the name and location of the theater; type of movies typically shown (e.g., first-run (current), second-run (budget), independent); type of theater (e.g., standard, drive-in, IMAX, dine-in); and movie ticket prices by category. b.List of theaters (and at what times) a movie is being shown. c.User reviews of the theaters. This should include an overall numeric rating of the theater and allow a written description..

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this question is for genetics Mendel finds a new species of pea plant that produces two different flower colors, black and white. He crosses together two parents, one homozygous recessive and one heterozygous parent for flower color (B= black, b= white). What is the probability that a plant in the F1 generation has black flowers? (Hint: Punnett squares are a great tool for these types of questions) 1/43/4 1 1/2.

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