2. The simplest approach to quantify gene
expression by RNA sequence to count the
number of reads that map to each gene
using different programs . Gene expression
is regulated both at mRNA and protein level.
Following are the
current methods
to quantify gene
expression:
Northern hybridization
,Ribonuclease
protection assay , RT
PCR
mRNA differential
assay ,SAGE and DNA
arrays
3. This is laboratory technique combining
reverse transcription of RNA to cDNA using
reverse transcriptase and amplification of
specific DNA targets using PCR.
The first involves including the reverse
transcriptase step in the same tube as
the PCR reaction(one step). The second
method involves creating complementary
DNA first by means of separate reverse
transcription reaction and then adding
the cDNA to the PCR reaction(two step)
