Here are the directions for making 50X Phosphate Buffer ( 1M; final concentration of
1X=20mM ), which is used to wash Dictyostelium cells.
36gNa2HPO488gNaH2PO4Bringto1.0LinddH2OpH=6.3 A. Write the chemical equation for the
equilibrium that describes this buffer. Identify the weak acid and the conjugate base. B. Calculate
the concentration of the two ingredients? C. Given these directions, is the final concentration of
the 50X solution 1M ? D. Is the 50X buffer the appropriate pH, given these directions? Use the
Henderson-Haselbalch equation to calculate the theoretical pH of the solution made according to
the directions. E. Tina's protocol for washing Dicty cells that have taken up bacteria states that
the cells must be washed in 1X phosphate buffer containing 5mM sodium azide. There is a bottle
of 1M sodium azide in the chemicals cabinet. How would you use 1M azide and 50X phosphate
buffer to make 500ml of the azide wash buffer?.
Here are the directions for making 50X Phosphate Buffer ( 1M; final c.pdf
1. Here are the directions for making 50X Phosphate Buffer ( 1M; final concentration of
1X=20mM ), which is used to wash Dictyostelium cells.
36gNa2HPO488gNaH2PO4Bringto1.0LinddH2OpH=6.3 A. Write the chemical equation for the
equilibrium that describes this buffer. Identify the weak acid and the conjugate base. B. Calculate
the concentration of the two ingredients? C. Given these directions, is the final concentration of
the 50X solution 1M ? D. Is the 50X buffer the appropriate pH, given these directions? Use the
Henderson-Haselbalch equation to calculate the theoretical pH of the solution made according to
the directions. E. Tina's protocol for washing Dicty cells that have taken up bacteria states that
the cells must be washed in 1X phosphate buffer containing 5mM sodium azide. There is a bottle
of 1M sodium azide in the chemicals cabinet. How would you use 1M azide and 50X phosphate
buffer to make 500ml of the azide wash buffer?
