SPIDES

2. Ahmed Samer
By
asamer83yousf@gmail.com Tel: 01066722201
General Hatchery Manager
El-Kasaby Group

4. After ovi-position, hatching eggs are first stored at the
breeder farm and then transported to the hatchery and
stored again before they are finally placed in an
incubator.
Egg temperatures should
decrease and increase
following a perfect ‘V’
pattern from the farm to
the hatchery.

5. The storage duration depends on:
The supply of hatching eggs.
 The hatchery capacity.
The market demand for day-old chicks.

6. It is well known that storage
duration more than 7 days:
increase incubation duration.
 decrease hatchability.
 decrease chick quality.
 decrease subsequent growth performance.
increase post-hatch mortality.

7. The changes in the micro-environment of
the embryo during storage:-
 CO2 and H2O are lost from the egg.
 The albumen PH increases from about 7.6 to about 9.0.
 The yolk PH increases from about 6.0 to about 6.5.
 The albumen height decreases.
 The strength of the yolk membrane decreases as well.

8. Over the years many techniques have been applied
to minimize losses from increased egg age :
Use of ‘buggy bags’ (covering eggs with plastic bags) to
minimize gaseous exchange.
Storing eggs upside down.
Turning eggs in storage.
High CO2 during storage.
 Low O2 during storage.
 Reduced storage temperatures.

9. During storage, eggs are stored at
temperatures below those necessary for
morphological development.
 However, some cellular activity, such as cell
death, still occurs in the embryo.

10. The chicks that do hatch will need a longer incubation
period, because the embryo will have had to grow from
the reduced number of live cells at the end of storage.

11. Most of the embryo mortality happens very early in
embryonic growth – at the membrane stage of
development.

12. One of the methods to reduce the
negative effects of long-term storage
has been to incubate eggs for short
periods during egg storage (SPIDES).

13. Objective of study
The specific goal of this study was to determine the effect
of Short Period of Incubation During Egg Storage on
hatchability and chick quality of broiler breeder eggs
stored for long periods.

14. Material
and
Methods

15. The experiment was
conducted on 5850
eggs (average 59.5 g)
Chiveer® breeder
farms of 34 weeks old
breeding flock.

16. Storage Periods Group No. Treatment
No.
Eggs No. Heating
Duration
Heating Times
Without Storage
( Fresh )
Group 1 T 0 450 0 0
7 Days Group 2 T 1 450 0 0
T 2 450 2.5 1
T 3 450 5 1
14 Days Group 3 T 4 450 0 0
T 5 450 2.5 2
T 6 450 5 2
21 Days Group 4 T 7 450 0 0
T 8 450 2.5 4
T 9 450 5 4
29 Days Group 5 T 10 450 0 0
T 11 450 2.5 5
T 12 450 5 5

18. Fertility
Hatchability.
Embryonic Mortality.
Incubation time.
Egg Weight Loss.

19. RESULTS
AND
DISCUSSION

20. Effects of storage periods on number of fertile eggs,
unfertile eggs and fertility:
3
8.66
6
2
7.33
141 139.66
121
63
0.66
94 93.11
80.66
42
0.44
0
20
40
60
80
100
120
140
160
T Fresh T 1 T 4 T 7 T 10
Unfertile eggs Fertile eggs Fertile%

21. Effects of SPIDES on number of fertile eggs,
unfertile eggs and fertility% :
3
8.66
3
4
6
6
6.33
2
3.66
2
7.33
7
4.33
141
139.66
142
142
121
138
134.66
63
134.33
126
0.66
101
68.66
94
93.11
94.66
94.66
80.66
92
89.77
42
89.55
84
0.44
67.33
45.77
T
FRESH
T 1 T 2 T 3 T 4 T 5 T 6 T 7 T 8 T 9 T 10 T 11 T 12
Unfertile eggs Fertile eggs Fertile%

22. Effects of storage periods and SPIDES on
hatchability and hatchability of fertile eggs
90.88
84
91.33
88.66
78.88
86.22
87.55
33.11
83.33
69.11
0
61.55
31.55
96.69
91.64
96.94
94.83
97.79
95.41
97.52
80.42
93.05
82.8
0
92.07
70.37
T
FRESH
T 1 T 2 T 3 T 4 T 5 T 6 T 7 T 8 T 9 T 10 T 11 T 12
Hatchability % Hatching% F.E

23. Effects of storage periods and SPIDES on chick
quality:
136.33
126
137
133
118.33
129.33
131.33
49.66
125
103.66
0
92.33
47.33
0
1.33
0.66
1
0
1.66
0
0
0
0.33
0
0.66
1
0
0.66
0
0.66
0
0.66
0
1
0
0.33
0
0
0
1 2 3 4 5 6 7 8 9 10 11 12 13
Chicks(A)
Chicks(B)
Chicks©

24. Effects of storage periods and SPIDES on
embryonic mortality:
6.66
6.33
2.33
6
23.66
7.66
10.33
84.66
12
22
142.33
39.66
77.33
1
2
1.33
0.66
0
1
0
0.33
3
10
0
0.66
4
2
3
2
2.33
1
2.33
2
4
5.66
10.33
0.33
7.66
10
T FRESH T 1 T 2 T 3 T 4 T 5 T 6 T 7 T 8 T 9 T 10 T 11 T 12
Early 0-7 Mid 8-14 Late 15-21

25. Effects of storage periods and SPIDES on
incubation time:
504.33
507.33
504.33 503.33
512.33
505.66 505.66
515.33
507.33 506.66
520.66
508.33 508.33
490
495
500
505
510
515
520
525
T Fresh T 1 T 2 T 3 T 4 T 5 T 6 T 7 T 8 T 9 T 10 T 11 T 12
incubation time /h

26. Effects of storage periods and SPIDES on
weight loss
0.168067227
0.515406162
0.593837535
0.515406162
0.941176471
0.929971989
1.042016807
1.770308123
1.714285714
1.837535014
2.06162465
2.352941176
2.420168067
12345678910111213
LOSS AFTER STORAGE %

30. What is the temperature should Stord Eggs
reach for SPIDES to be successful?
Eggshell temperatures need
to reach a temperature of
38.3°C (101°F) for SPIDES
to be effective

31. Does it matter how long the eggs take to warm
up?
The time taken to reach 32°C (90°F) can range from 2 hours
to 8 hours, depending on the type of machine used and how
full it is.
The time taken to warm the eggs above 32°C (90°F) does not
have any effect on the outcome of SPIDES – fast or slow
heating times can both be effective.

32. Conclusions
Our study provides evidence that long term storage of
eggs (21 and 29 days) adversely affects all hatching
traits. It is recommended that when storage of eggs
more than seven days is urgently important; one
should warm eggs every five days during storage to
minimize the harmful impact of storage.