1. Objective 5: Cross-crop issues
Activity 1: Drought phenotyping
= Refining breeder tools
Proposed Workplan for Phase II
ICRISAT – CIAT – ISRA – Univ North Carolina – NARS
of different crops

2. Background and rationale for that activity
No cross specie phenotyping in Phase I but comparative
genomics
Different crops work on similar traits with different methods
Incomplete / unrefined breeder tool box
No clear idea of trait value on yield
Capacity building (human) on drought phenotyping needed

3. What traits?
Reproduction /
partitioning
Water use
/ productivity
Jw
Water
uptake

4. Proposed sub-Activities
Sub-Activity 5: Training
Water use / productivity
Water uptake
Reproduction and partitioning
Modeling

5. How to improve / enlarge / refine
the breeder tool box?
“Breeder toolbox”
SPAD / SLA
CID
Staygreen
CTD
Grain number /
Grain size
Relation with yield
Relation with essential processes whose effects can be modeled
Prediction of trait / process value on yield
More / better tools

6. (Link with Trait phenotyping + phenot protocols
Services of MBP)
“Breeder toolbox”
SPAD / SLA
CID
Staygreen
CTD
Grain number /
Grain size

7. (Link with Trait phenotyping + phenot protocols Services of MBP)
“Water uptake” “Water use /
“Breeder toolbox” Productivity”
High TE
SPAD / SLA
High mesophyll capacity
CID
Low Gs
Staygreen
Stomata sensitivity
CTD to VPD
Grain number / Pre/post -anthesis
Grain size water use
“Reproduction / Remobilization”

8. “Water uptake” “Water use /
“Breeder toolbox” Productivity”
High TE
SPAD / SLA
High mesophyll capacity
CID
Low Gs
Staygreen
Stomata sensitivity
CTD to VPD
Grain number / Pre/post -anthesis
Grain size water use
“Reproduction / Remobilization”

9. “Water uptake” “Water use /
“Breeder toolbox” Productivity”
High TE
SPAD / SLA
“Right” kinetics of High mesophyll capacity
water uptake CID
Low Gs
Staygreen
Higher water Stomata sensitivity
uptake CTD to VPD
Grain number / Pre/post -anthesis
Grain size water use
“Reproduction / Remobilization”

10. “Water uptake” “Water use /
“Breeder toolbox” Productivity”
High TE
SPAD / SLA
“Right” kinetics of High mesophyll capacity
water uptake CID
Low Gs
Staygreen
Higher water Stomata sensitivity
uptake CTD to VPD
Grain number / Pre/post -anthesis
Grain size water use
Flower “tolerance” Shorter/longer flower PPI / PHI
to water stress to maturity interval SBR / HI
“Reproduction / Remobilization”

11. “Water uptake” “Water use /
“Breeder toolbox” Productivity”
“Right” kinetics of
water uptake ?
? Low Gs
Staygreen
Higher water ?
? Stomata sensitivity
uptake to VPD
?
Pre/post -anthesis
water use

12. Does my tool relate to yield?

13. How does the tool relate to a process?

14. “Water uptake”
“Breeder toolbox”
“Right” kinetics of
water uptake
Staygreen

15. Testing traits by
Crop simulation modeling
“Water uptake”
“Breeder toolbox”
“Right” kinetics of
water uptake
Staygreen

16. Probability of yield increase – Trait A
75%
Median
25%

17. Probability of yield increase – Trait B
75%
Median
25%

18. Testing traits by
Crop simulation modeling
“Breeder toolbox”
Trait A
Trait B

19. Testing traits by
Crop simulation modeling
“Water uptake” “Water use /
“Breeder toolbox” Productivity”
High TE
SPAD / SLA
“Right” kinetics of High mesophyll capacity
water uptake CID
Low Gs
Staygreen
Higher water Stomata sensitivity
uptake CTD to VPD
Grain number / Pre/post -anthesis
Grain size water use
Flower “tolerance” Shorter/longer flower PPI / PHI
to water stress to maturity interval SBR / HI
“Reproduction / Remobilization”

20. Outputs to TLII
Sub-Activity 5: Training
Water use / productivity
Water uptake
Reproduction and partitioning
Modeling
Trait value Refined protocols Better pheno-
predicted More tools typing data

21. Thank you