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CV of Agnieszka Zygadlo Nielsen



                     Biotechnologist with multi-European background

Personal Information
Name:          Agnieszka Zygadlo Nielsen                    Phone:         (+45) 61 67 70 20
Birthday:      June 27, 1978                                Email:         azn@comxnet.dk
Nationality:   French                                       Address:       Landehjælpvej 14
Family:        Married to Per Lassen Nielsen(Danish)                       2300 København S
               Two sons

Résumé
          I am an educated research scientist with a broad background in plant biotechnology. I hold two masters
8Genetics and Oncology) and a PhD degree in biotechnology. After a one year post doc, I worked in a medium scale
biotech company for two years. Due to the closing down of the company, I have been working at Aarhus and
Copenhagen University since then. However, after establishing my family, I realize I would like to change career
towards work in a biotech company.
          My relevant personal skills are that I am dedicated and focused at work combined to a high sense of being a
team player with a great sense of humor.I am energetic, well organized, open minded and I appreciate being in an
international environment. I like searching and coming up with solutions to different new tasks.

Educational Background
2012:          Project management module 1 and 2 theory completed at Implement consulting
               PMP certification in process

2005:          PhD degree in molecular biology
               Faculty ofLife Sciences, University of Copenhagen, Denmark

2001:          Postgraduate degree in cellular biology and physiology, obtained with honours
               University D.Diderot, Paris VII, France

2000:          MSc in cancer research and additional research engineer degree (Magistère) in Genetics
               University D.Diderot, Paris VII, France

1996:          High School Diploma (Baccalauréatscientifique)
               Lycée Louis Bascan, Rambouillet, France

Employmentexperience

since april 2010: Scientist, Faculty of Life Sciences, Plant biochemistry laboratory, University of Copenhagen in
                  Prof. Poul Erik Jensen and Prof. Birger Lindberg Møller groups in the synthetic biology UNIK
                  project

2009-2010:        Scientist, Aarhus University, Frederiksberg in Prof. Peter Ulvskov’s group in the Renewall
                  European biofuel project

2007-2008:        Research scientist atAresa A/S

2006:             Post-doc, Faculty of Life Sciences, Plant biochemistry laboratory, University of Copenhagen
CV of Agnieszka Zygadlo Nielsen


2002-2005:        PhDstudent, Faculty of Life Sciences, University of Copenhagen in Prof. Henrik V. Scheller’s
                  group

2001:             Master student in the Unit of Papillomaviruses, skin cancer department,Institut Pasteur, Paris


Work Experience

          Solid Background in Research
Field of experience:biotechnology, oncology, genetics, biochemistry, molecular biology, biofuels, synthetic biology.
Main experimental skills in keywords:protein expression, enzymatic reactions, protein analysis, protein and RNA
purification plant compound purification -radio labeling –in vitro transcription/ translation,cloning,cell fractionation,
GMOs,antibodies

         Excellent Documentation, Communication & Presentation Skills
Writing and planning of several scientific articles published in high ranking international scientific journals.
Oral and Poster presentations at several international scientific conferences.
Numerous oral presentations in English and French.

        Experience in Teaching& Project Planning
2006-2008: Coaching and project planning of work for to 2 laboratory technicians
2002-2010: Coaching and project planning of PhD, MSc and Bsc students

        Experience in Course Teaching
2002-2005: Teaching MSc course in Experimental Molecular Biology, and PhD course in Advances in Plant
            Biology at the Faculty of Life Sciences, University of Copenhagen

         Excellent IT Skills
Expert user of Microsoft Office, diverse bioinformatics databases, and various programs for sequence analysis,
bioinformatics and instrumentcontrol.Profound experience in literature and database searches.

Languages
French:        Native                         English: Fluent, written and oral
Polish:        Native                         Danish: fluent on conversation level

International Experience
2003:          1 month of hiking in Nepal and Tibet
2000:          6 months project as Erasmus student at the University of Copenhagen
1999:          3 months project at the University of Edinburgh
1998:          2 months work as volunteer for the sea turtle protection society in Greece

Scholarships
2005:          Grant for participation in the EMBO course Cellular and Molecular Biology of Membranes, Cargese,
               France

2004:          Grant for participation in the Forum for Young Scientists and FEBS congress, Warsaw, Poland
CV of Agnieszka Zygadlo Nielsen


2002:         Marie Curie FP5 PhD scholarship as part of the PSI consortium at the Faculty of Life Sciences,
              University of Copenhagen

1998-2002:    University of Paris scholarship for MSc students in oncogenesis



Visions for Future Professional Development
Combining my scientific background with business, strategy and communication
Being challenged by exciting work
Working in a team of motivated colleagues towards a common goal
Working in a company in which professional development is possible within thecompany

Spare Time Activities
Family - Travelling -Reading -Cinema-Swimming

Personal References
Christina Lunde, Scientist/ Project manager
Novozymes
Christina.lunde@gmail.com

Iben Damager, Scientist/ Project leader
Novozymes
ibdg@novozymes.com

Birger Lindberg Møller, Professor
University of Copenhagen, Dept. of biotechnology and plant biology
blm@life.ku.dk

List of publications
   1.   Zygadlo A, Jensen PE, Leister D &Scheller HV (2005) Photosystem I lacking the PSI-G subunit has higher
        affinity for plastocyanin and is sensitive to photodamage. Biochim. Biophys. Acta. 1708

   2.   Zygadlo A, Rosgaard L, Scheller HV, Mant A & Jensen PE (2005) Insertion of the plant photosystem I
        subunit G into the thyklakoid membrane: in vitro and in vivo studies of wild-type and tagged versions of the
        protein. FEBS J. 272

   3.   Kouril R, Zygadlo A, Artenia AA, de Wit CD, Dekker JP, Jensen PE, Scheller HV &Boekema EJ (2005)
        Structural characterization of a complex of photosystem I and light-harvesting complex II of Arabidopsis
        thaliana. Biochemistry

   4.   Zygadlo A, Robinson C, Scheller HV, Mant A, Jensen PE (2006) The properties of the positively charged
        loop region in PSI-G are essential for its “spontaneous” insertion into thylakoids and rapid assembly into the
        photosystem I complex. J. Biol. Chem.

   5.   Hansson A, Amann K, Zygadlo A, Meurer J, Scheller HV, Jensen PE (2007) Knock-out of the chloroplast-
        encoded PSI-J subunit of photosystem I in Nicotianatabacum. FEBS J.

   6.   Glaring MA, Zygadlo A, Thorneycroft D, Schulz A, Smith SM, Blennow A, Baunsgaard L (2007) An extra-
        plastidialα-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not
        necessary for transient starch degradation J.Exp.Bot.
CV of Agnieszka Zygadlo Nielsen


   7.   Lunde C, Zygadlo A, Simonsen HT, Nielsen P, Blennow A, Haldrup A (2008) Sulfur starvation in rice: the
        effect on photosynthesis, carbohydrate metabolism, and oxidative stress protective pathways.
        PhysiologiaPlantarum

   8.   Fristedt R, Carlberg I, Zygadlo A, Piippo M, Nurmi M, Aro EM, Scheller HV, Vener A (2009) Intrinsically
        Unstructured Phosphoprotein TSP9 Regulates Light Harvesting in Arabidopsis thaliana. Biochemistry


   9.   Zygadlo Nielsen A, Ziersen B, Jensen K, Møller BL, Jensen PE. (2012) Light driven P450 enzymatic
        reactions. Submitted to ACS synthetic biology

Additional Information
Please do not hesitate to contact me for further information including MSc and PhD diplomas and international
conference participations.

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  • 1. CV of Agnieszka Zygadlo Nielsen Biotechnologist with multi-European background Personal Information Name: Agnieszka Zygadlo Nielsen Phone: (+45) 61 67 70 20 Birthday: June 27, 1978 Email: azn@comxnet.dk Nationality: French Address: Landehjælpvej 14 Family: Married to Per Lassen Nielsen(Danish) 2300 København S Two sons Résumé I am an educated research scientist with a broad background in plant biotechnology. I hold two masters 8Genetics and Oncology) and a PhD degree in biotechnology. After a one year post doc, I worked in a medium scale biotech company for two years. Due to the closing down of the company, I have been working at Aarhus and Copenhagen University since then. However, after establishing my family, I realize I would like to change career towards work in a biotech company. My relevant personal skills are that I am dedicated and focused at work combined to a high sense of being a team player with a great sense of humor.I am energetic, well organized, open minded and I appreciate being in an international environment. I like searching and coming up with solutions to different new tasks. Educational Background 2012: Project management module 1 and 2 theory completed at Implement consulting PMP certification in process 2005: PhD degree in molecular biology Faculty ofLife Sciences, University of Copenhagen, Denmark 2001: Postgraduate degree in cellular biology and physiology, obtained with honours University D.Diderot, Paris VII, France 2000: MSc in cancer research and additional research engineer degree (Magistère) in Genetics University D.Diderot, Paris VII, France 1996: High School Diploma (Baccalauréatscientifique) Lycée Louis Bascan, Rambouillet, France Employmentexperience since april 2010: Scientist, Faculty of Life Sciences, Plant biochemistry laboratory, University of Copenhagen in Prof. Poul Erik Jensen and Prof. Birger Lindberg Møller groups in the synthetic biology UNIK project 2009-2010: Scientist, Aarhus University, Frederiksberg in Prof. Peter Ulvskov’s group in the Renewall European biofuel project 2007-2008: Research scientist atAresa A/S 2006: Post-doc, Faculty of Life Sciences, Plant biochemistry laboratory, University of Copenhagen
  • 2. CV of Agnieszka Zygadlo Nielsen 2002-2005: PhDstudent, Faculty of Life Sciences, University of Copenhagen in Prof. Henrik V. Scheller’s group 2001: Master student in the Unit of Papillomaviruses, skin cancer department,Institut Pasteur, Paris Work Experience Solid Background in Research Field of experience:biotechnology, oncology, genetics, biochemistry, molecular biology, biofuels, synthetic biology. Main experimental skills in keywords:protein expression, enzymatic reactions, protein analysis, protein and RNA purification plant compound purification -radio labeling –in vitro transcription/ translation,cloning,cell fractionation, GMOs,antibodies Excellent Documentation, Communication & Presentation Skills Writing and planning of several scientific articles published in high ranking international scientific journals. Oral and Poster presentations at several international scientific conferences. Numerous oral presentations in English and French. Experience in Teaching& Project Planning 2006-2008: Coaching and project planning of work for to 2 laboratory technicians 2002-2010: Coaching and project planning of PhD, MSc and Bsc students Experience in Course Teaching 2002-2005: Teaching MSc course in Experimental Molecular Biology, and PhD course in Advances in Plant Biology at the Faculty of Life Sciences, University of Copenhagen Excellent IT Skills Expert user of Microsoft Office, diverse bioinformatics databases, and various programs for sequence analysis, bioinformatics and instrumentcontrol.Profound experience in literature and database searches. Languages French: Native English: Fluent, written and oral Polish: Native Danish: fluent on conversation level International Experience 2003: 1 month of hiking in Nepal and Tibet 2000: 6 months project as Erasmus student at the University of Copenhagen 1999: 3 months project at the University of Edinburgh 1998: 2 months work as volunteer for the sea turtle protection society in Greece Scholarships 2005: Grant for participation in the EMBO course Cellular and Molecular Biology of Membranes, Cargese, France 2004: Grant for participation in the Forum for Young Scientists and FEBS congress, Warsaw, Poland
  • 3. CV of Agnieszka Zygadlo Nielsen 2002: Marie Curie FP5 PhD scholarship as part of the PSI consortium at the Faculty of Life Sciences, University of Copenhagen 1998-2002: University of Paris scholarship for MSc students in oncogenesis Visions for Future Professional Development Combining my scientific background with business, strategy and communication Being challenged by exciting work Working in a team of motivated colleagues towards a common goal Working in a company in which professional development is possible within thecompany Spare Time Activities Family - Travelling -Reading -Cinema-Swimming Personal References Christina Lunde, Scientist/ Project manager Novozymes Christina.lunde@gmail.com Iben Damager, Scientist/ Project leader Novozymes ibdg@novozymes.com Birger Lindberg Møller, Professor University of Copenhagen, Dept. of biotechnology and plant biology blm@life.ku.dk List of publications 1. Zygadlo A, Jensen PE, Leister D &Scheller HV (2005) Photosystem I lacking the PSI-G subunit has higher affinity for plastocyanin and is sensitive to photodamage. Biochim. Biophys. Acta. 1708 2. Zygadlo A, Rosgaard L, Scheller HV, Mant A & Jensen PE (2005) Insertion of the plant photosystem I subunit G into the thyklakoid membrane: in vitro and in vivo studies of wild-type and tagged versions of the protein. FEBS J. 272 3. Kouril R, Zygadlo A, Artenia AA, de Wit CD, Dekker JP, Jensen PE, Scheller HV &Boekema EJ (2005) Structural characterization of a complex of photosystem I and light-harvesting complex II of Arabidopsis thaliana. Biochemistry 4. Zygadlo A, Robinson C, Scheller HV, Mant A, Jensen PE (2006) The properties of the positively charged loop region in PSI-G are essential for its “spontaneous” insertion into thylakoids and rapid assembly into the photosystem I complex. J. Biol. Chem. 5. Hansson A, Amann K, Zygadlo A, Meurer J, Scheller HV, Jensen PE (2007) Knock-out of the chloroplast- encoded PSI-J subunit of photosystem I in Nicotianatabacum. FEBS J. 6. Glaring MA, Zygadlo A, Thorneycroft D, Schulz A, Smith SM, Blennow A, Baunsgaard L (2007) An extra- plastidialα-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation J.Exp.Bot.
  • 4. CV of Agnieszka Zygadlo Nielsen 7. Lunde C, Zygadlo A, Simonsen HT, Nielsen P, Blennow A, Haldrup A (2008) Sulfur starvation in rice: the effect on photosynthesis, carbohydrate metabolism, and oxidative stress protective pathways. PhysiologiaPlantarum 8. Fristedt R, Carlberg I, Zygadlo A, Piippo M, Nurmi M, Aro EM, Scheller HV, Vener A (2009) Intrinsically Unstructured Phosphoprotein TSP9 Regulates Light Harvesting in Arabidopsis thaliana. Biochemistry 9. Zygadlo Nielsen A, Ziersen B, Jensen K, Møller BL, Jensen PE. (2012) Light driven P450 enzymatic reactions. Submitted to ACS synthetic biology Additional Information Please do not hesitate to contact me for further information including MSc and PhD diplomas and international conference participations.