1) Describe and interpret the data in Figure 2.1. Describe: What was measured? What are the patterns in the data? Interpret: What can you reasonably infer or conclude from the data? For example, this will often be a statement of the data suggest that X is necessary for Y. 2) What roles do the Mizm1 DNA sequence and the Miz-1 protein seem to be playing, according to the researchers results? Explain your reasoning using model of transcriptional initiation in eukaryotes in Figure 2.2 (below). Figure 2.1: Reporter plasmid. To study how specific DNA sequences help control gene expression, researchers create reporter plasmids. A reporter plasmid is a small piece of DNA engineered in the laboratory. Researchers cut out the DNA sequence(s) they want to study and paste it in front of a gene encoding a protein that is easily detected in the laboratory. In this case, the researchers use the luciferase gene, which encodes an enzyme that catalyzes a light-emitting reaction that is easily studied in the laboratory. The researchers created a reporter plasmid that had one of their DNA sequences of interest, which they called Mizm1 ("miz motif 1"), in front of a luciferase reporter gene (shown on the left). They also created a control plasmid that did not have the Mizm1 sequence, but did have the luciferase gene shown on the right). Figure 2.1. The researchers put either the reporter plasmid (pGL3e-Mizm1) or the control plasmid (pGL3ec) into cells from a human cell line (HeLa). They compared luciferase expression in cells with each plasmid that either did (Miz-1) or did not (EV) also contain the Miz-1 protein. eaux, B. L., Burow, D., Lockwood, S. H., Yu, A., Segal, D. J., & Knoepfler, P. S.Figure 2.2: Model of transcription initiation in eukaryotes..

1. 1) Describe and interpret the data in Figure 2.1. Describe: What was measured? What are
the patterns in the data? Interpret: What can you reasonably infer or conclude from the
data? For example, this will often be a statement of the data suggest that X is necessary for
Y.
2) What roles do the Mizm1 DNA sequence and the Miz-1 protein seem to be playing,
according to the researchers results? Explain your reasoning using model of
transcriptional initiation in eukaryotes in Figure 2.2 (below).
Figure 2.1: Reporter plasmid. To study how specific DNA sequences help control gene
expression, researchers create reporter plasmids. A reporter plasmid is a small piece of DNA
engineered in the laboratory. Researchers cut out the DNA sequence(s) they want to study and
paste it in front of a gene encoding a protein that is easily detected in the laboratory. In this case,
the researchers use the luciferase gene, which encodes an enzyme that catalyzes a light-emitting
reaction that is easily studied in the laboratory. The researchers created a reporter plasmid that
had one of their DNA sequences of interest, which they called Mizm1 ("miz motif 1"), in front of a
luciferase reporter gene (shown on the left). They also created a control plasmid that did not have
the Mizm1 sequence, but did have the luciferase gene shown on the right). Figure 2.1. The
researchers put either the reporter plasmid (pGL3e-Mizm1) or the control plasmid (pGL3ec) into
cells from a human cell line (HeLa). They compared luciferase expression in cells with each
plasmid that either did (Miz-1) or did not (EV) also contain the Miz-1 protein. eaux, B. L., Burow,
D., Lockwood, S. H., Yu, A., Segal, D. J., & Knoepfler, P. S.Figure 2.2: Model of transcription
initiation in eukaryotes.