Explanation: 1. a. Draw a malonyl-CoA chain-propagating unit for polyketide biosynthesis. b. Youve decided the label this malonyl chain-propagating unit with 13C at what would be the C1 carbon of acetyl-CoA. Indicate the carbon isotope(s) on your drawing with an asterisk (*). c. Circle the carbon that will be decarboxlated during the claisen condensation. 2. Two modules of the PKS that makes avermectin are shown, as well as the final polyketide product. What would the final product look like if we mutated the dehydratase (DH) domain in module 2 such that it no longer worked? You only need to redraw the highlighted region of the molecule. b. What would the final product look like if we added an enoyl reductase and mutated the keto reductase domain module 2 so that it was no longer functional? You only need to draw that part of the molecule a Loading AVE A1 module module 1 module 2.

1. Explanation:
1. a. Draw a malonyl-CoA chain-propagating unit for polyketide biosynthesis. b. Youve decided the
label this malonyl chain-propagating unit with 13C at what would be the C1 carbon of acetyl-CoA.
Indicate the carbon isotope(s) on your drawing with an asterisk (*). c. Circle the carbon that will be
decarboxlated during the claisen condensation.
2. Two modules of the PKS that makes avermectin are shown, as well as the final polyketide
product. What would the final product look like if we mutated the dehydratase (DH) domain in
module 2 such that it no longer worked? You only need to redraw the highlighted region of the
molecule. b. What would the final product look like if we added an enoyl reductase and mutated
the keto reductase domain module 2 so that it was no longer functional? You only need to draw
that part of the molecule
a Loading AVE A1 module module 1 module 2