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1. Synthetic plant virology for Nanobiotechnology
and Nanomedicine
Presented by:
Abhinay singh
PG/M-4240/18
M.Sc Biotechnology
2. NANOTECHNOLOGY
ï” The design, characterization, production
and application of structures, devices and
systems by controlling shape and size at the
nanoscale
4. âAdaptability to manipulate, control, assemble, produce and
manufacture things at atomic precisionâ (THEREâS PLENTY OF ROOM AT
THE BOTTOM )
Richard feynman
âThefatherof Nanotechnologyâ
5. Norio Taniguchi, Professor
- coined the term âNanotechnologyâ(1974)
âNano- technologyââ - processing, separation,
consolidation and deformation of materials by
one atom or by one molecule".
6. Properties Of Nanoparticles
ï¶ Nanoparticles show unusual physical,chemical and biological
properties,which are completely lacked in their bulk molecule.
ï¶ Nanoparticles have a high surface to volume ratio that increase
their reactivity and biochemical activity.
ï¶ Nanoparticles possess strong affinity to targets such as proteins.
9. Chemical method
ï¶ Chemical reduction method :In this method, a copper salt is reduced
by a reducing agent such as sodium borohydride, ascorbic acid into
copper nanoparticle.
ï¶ Sonochemical method : In this method powerful ultrasound radiation
of 10 -20 Hz are applied to molecule to enhance the chemical
reaction.
10. Physical method
âą Electro chemicalmethod :In this method electricity is
usedas the driving or controlling force to produce
nanoparticlesof metal.
11. Biological Synthesis Of Nanoparticles
âą Microbial synthesis of nano particle: Numerous
micro organism have been found to produce
nanoparticles in the substrate.
11/27/18
13. Photosynthesis Of Nanoparticles
Plant extracts can also serve as one of the effective and eco
friendly material for synthesis of nanoparticles. E.g extract
from Ocimum tenuiflorum ,Solanum tricobatum,Citrus
sinensis were used for synthesis of silver nanoparticlesfrom
silver nitrate solution.
17. In Vitro Assembly of Structures - Bioconjugation
Figure
Representations of novel VNP structures for functionalization. (a)
TMVâderived ânanoâboomerangâ (b) TMV tetrapod, both derived
by in vitro formation mediated by either two or four OAS on a
single RNA. (c) Nanoâstar formed by conjugating multiple TMV
OAS to a gold nanoparticle. (d) Catalytically active TuMV nanonet
formed by conjugation to C. antarcticaLipase B.
18. Figure 3
Schematic of the key methods used to encapsulate specific cargoes into VNPs. Left
shows swellingâmediated infusion of nanoparticles. Right demonstrates cargo caging.
ENCAPSULATION
20. Virus Summary Reference
Hepatitis B Tabletized transgenic lettuce containing HBsAg VLPs
is orally immunogenic in mice
Pniewski 2011136
Hepatitis C Cucumber mosaic virus nanoparticles carrying a
Hepatitis C virusâderived epitope, orally immunogenic
in rabbits
Nuzzaci 2010137
Hepatitis C Papaya mosaic virusâlike particles fused to a hepatitis
C virus epitope: evidence for the critical function of
multimerization, mixed response in mice
Denis 2007138
Influenza Influenza virusâlike particles induce a protective
immune response against a lethal viral challenge in
mice, produced for H7N9 outbreak virus
D'Aoust 2008139
Papillomavirus HPVâ16 L1 VLPs via agroinfiltrationâmediated
transient expression or via transplastomic expression
Maclean 2007,140FernandezâSan 2008141
Papillomavirus Expression of HPVâ8 L1 VLPs Matic 2012142
Papillomavirus transient expression of chimaeric L1::L2 VLPs and
proof of increased breadth of immune response
Pineo 2013143
Bovine papillomavirus Transient expression of BPV L1 VLPs Love 2012144
21. Scope Of Nanotechnology
ï¶ Nanotechnology can be used as alternative tool for
existing plant disease management.
ï¶ Nanotechnology can be used for combating the plant
disease either by controlled delivery of functional
compounds or as diagnostic tool for disease .
ï¶ Importance of nanoscale delivery system in agriculture is
because of its improved solubility and degradation in the
environmental factors.
22. Conclusion
ï¶ Use of nanoparticles in plant disaese management is a novel
and fancy approach that may prove in future with the
progress of application aspect of nanotechnology.
ï¶ Despite of tremendous application scope of nanotechnology in
plant disease management ,there are certain demerits, risk and
apprehension in the use of NPs in agriculture which are
required to be worked out on priority and before the
commercial use of nanotechnology in agriculture
23. REFERENCES
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