ppt

1. AN AUTO-PERFUSING
UMBILICAL CORD BLOOD
COLLECTION INSTRUMENT

2.  Contents
 Introduction
 Why this advancement in the field of
‘Biomedical Instrumentation ‘?
 UCB Collection Instrument
 Systems in UCB collection Instrument
 Two Fraction UCB collection
 Applications
 Conclusion

3.  Introduction
 An Automated Blood collection Instrument
comprises of Mechanical ,electronic and
Control components.
 UCB from the placenta provides a rich
source of highly proliferative cells for many
clinical uses as it contains rich
Hematopoietic Stem Cells (HSCs) .
 Current collection of UCB uses a syringe to
extract blood from placenta.
 An Automated UCB collection instrument to

4. Why this advancement in the
field of ‘Biomedical
Instrumentation ‘?
As an ex-utero approach, the challenges to
be addressed during the collection
process will include:
(1) Limited time window for further collection.
(2) Need to stay robust of cross
contamination from maternal blood.
(3) Replication of a uterus compression force
on the placenta.
(4) In Ex-Utero,infusion of saline back into
the placenta will dislodge the cells

5. Ex-Utero

6.  UCB Collection Instrument
Functionally, it comprises the following
systems:
• Placenta handling system.
• Controlled air chamber.
• Auto-perfusion system.
• Time window widening system.
• Open-architecture control system.

8.  Placenta Handling System
Main parts of the system :
• Air tight lid
• Placenta Bowl
• Supporting Legs
• Base

9.  Parts Of The System

11.  Controlled Air Chamber
The lid houses the interfaces to three
standard ports which are connected to
• Proportional Valve
• Solenoid valve
• An Analog Pressure Sensor

13. • The air pressure variation in the chamber will be converted
to a patterned force profile acting on the maternal surface of the
placenta via a pressure membrane as shown in Fig.

14. Finite Element Analysis(FEA)

15.  Auto-Perfusing System
• Enables perfusion automatically.
• Consist of catheter and three valves.
• A catheter is inserted into a vein on
the umbilical cord.
• Other end of the catheter a three-way
valve is connected.

17.  Time -Widening Window
System
 A two-pronged approach is adopted.
 A vibrator, rendering a low frequency
vibration of about 2 Hz.
 Secondly, the exposed end of the cord
can be rapidly cooled to a low
temperature once it is cut.

19.  Open –Architecture Control
System
 Needed for all components to work
together as one unified instrument.
 A standalone embedded control
system is employed based on the
National Instrument (NI) single board
solution sbRIO-9632 .

21. The I/O interface from the board to the other
components is depicted in fig below :

23.  Two-fraction UCB collection
 Synchronous operations with current
procedures of code blood banks along
with autoperfusion is also possible.

24. The flowchart of the overall automated procedure is
shown in fig

26.  Conclusion
 An automated and self-perfusing
instrument enables the more efficient
and extensive harvesting of HCS.
 Can be used for the pediatric and
adult treatment.
 Instrument maximizes the explusion of
cord blood.
 a stand-alone system to retrieve cord
blood in a one stage collection

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