The Personal Genome Machine™ advances Next-Generation sequencing to PostLight™ sequencing—the translation of chemical sequence information directly into digital form. PostLight™ technology means genetic sequencing is leveraged by the extreme scalability of semiconductor technology. Moore’s law is applied to genetic analysis. For more information visit: https://products.invitrogen.com/ivgn/product/4462917?CID=PGMbrochure2-SS-12312

1. Ion Torrent
The Chip is the Machine
™
Ion Torrent
The Chip is the Machine
scalability
simplicity
speed

2. Scalability
massively scalable semiconductor technology
Simplicity
natural biochemistry, no optical components
Speed
runs in about two hours, not days or weeks
Discover Ion Torrent
The Ion Personal Genome Machine™ (PGM™) is simpler, more cost-effective, and more scalable than any other sequencing
technology. The PGM™ sequencer is a benchtop system utilizing groundbreaking and disruptive semiconductor technology
that enables rapid and scalable sequencing experiments.
Ion Torrent technology uses a massively parallel array of proprietary semiconductor sensors to perform direct, real-time
measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the Ion semicon-
ductor chips provides millions of individual reactors, and integrated fluidics allow reagents to flow over the sensor array.
This unique combination of fluidics, micromachining, and semiconductor technology enables the direct translation of
genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high-quality data.
The Personal Genome Machine™, along with Ion Torrent semiconductor chips, Ion Torrent reagent kits, and the Torrent
Server/Torrent Suite software, allow Ion Torrent to deliver a cutting-edge sequencing solution.

3. Discover scalability Discover simplicity Discover speed
scales like a semiconductor simple chemistry direct, real-time detection technology
• Leverages $1 trillion semiconductor • Simplest sequencing chemistry: • Makes sequencing accessible to
industry investment hydrogen ion detection (pH meter) new applications and markets
• Scales by density: 40 cumulative • Natural nucleotides: no enzymatic • Short run time supports fast
years of Moore’s Law cascade, no fluorescence, and no research cycle and faster time to
• Scales by volume: 400 standard chemiluminescence publication
semiconductor foundries from • Low-cost, standard reagents • The Chip is the Machine™: no optics,
which to choose no light, no scanning
Sequencing and analysis pipeline
The Ion Torrent™ sequencing workflow consists of a number of standard
elements—creation of a DNA or RNA library, a simple PCR process creating
amplified templates on proprietary Ion Sphere™ particles, followed by a sequenc-
ing run on the PGM™ platform.
With the completion of a PGM™ sequencer run, data automatically transfer to the
dedicated Torrent Server that runs Torrent Suite software. Here, raw Ion signals
are converted to base calls and stored in industry-standard SFF or FASTQ files.
These data can be processed by a variety of commercially available software
packages for applications such as variant detection, RNA-Seq,
ChIP-Seq, or de novo genome assembly.

4. Specifications
Ion 314™ Chip
Scale 1.3 million wells
Data output Starting at 10 Mb of high-quality sequence*
Ion 316™ Chip
Scale 6.3 million wells
Data output Starting at 100 Mb of high-quality sequence*
Ion 318 Chip
™ †
Scale ~12 million wells
Data output Starting at 1 Gb of high-quality sequence*
For all chips
Data output SFF/FASTQ file output
Reagents Complete sets of reagents, including library, template, and sequencing kits
Applications Amplicon sequencing, microbial sequencing, RNA-Seq, ChIP-Seq, targeted resequencing, copy number
analysis, methylation, paired-ends
Ordering information
Description Part No.
Personal Genome Machine™
Ion PGM™ System 4462921
Includes PGM sequencer (4462917) and Torrent Server (4462918)
™
Service Contracts
AB Assurance Plan ZG11SCIONPGMSYS
Rapid Exchange Program AB Assurance Plan ZGEXSCIONPGMSYS
Semiconductor Sequencing Chips
Ion 314™ Chip Kit (8 chips) 4462923
Ion 316 Chip Kit (4 chips)
™
4466616
Ion 316 Chip Kit (8 chips)
™
4469496
Reagent Kits
Ion Xpress™ Fragment Library Kit (up to 20 reactions) 4468987
Ion Fragment Library Kit (up to 20 reactions) 4466464
Ion Total RNA-Seq Kit (12 reactions) 4466666
Ion DNA Barcode 1-16 Kit† (10 sets of 16 libraries) 4468654
Ion Xpress™ Template Kit (10 reactions) 4469001
Ion Sequencing Kit (8 reactions) 4468997
Ion Control Kits
Ion Control Materials Kit (3 reactions each per control) 4466465
Ion Sphere™ Quality Control Kit (20 measurements) 4468656
Ion Library Quantitation Kit (250 reactions) 4468802
* Throughput calculated with Escherichia coli DH10B genome, using Ion Sequencing 314 chemistry and a 100 bp read length PGM™ sequencer configuration.
† The content provided herein may relate to products that have not been officially released and is subject to change without notice.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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