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Introduce how to development RNA delivery platform and methods
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PEG10 mRNA.pptx
1.
2.
3.
Domain architectures of
selected capsid (CA)–containing mammalian Gag homologs CA: capsid NC: nucleocapsid PR: protease RT: reverse transcriptase LTR: long terminal repeat MA: matrix IN: integrase
4.
Capsid (CA):the protein
shell of a virus
5.
TEMs and Cyto
TEM of the Mm orthologs of the CA domain–containing proteins
6.
Quantification of extracellular
CA domain–containing proteins
7.
MmPEG10 protein and
mRNA are secreted in vesicles by cells in vitro
8.
Key domains for
RNA-binding
9.
5-UTR and 3-UTR
are the main binding site for MmPEG10
10.
Flanking mRNA with
MmPeg10 5 and 3 UTRs enables functional intercellular transfer of mRNA into a target cell
11.
SEND: selective endogenous
encapsidation for cellular delivery
12.
True SEND: selective
endogenous encapsidation for cellular delivery
13.
PEG10 is a
modular platform for RNA delivery
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