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Molecular weight can be obtained from a very small sample. It does not involve the absorption or emission of light. A beam of high-energy electrons breaks the molecule apart. The masses of the fragments and their relative abundance reveal information about the structure of the molecule DETERMINE THE MOLECULAR MASS OF ORGANIC COMPOUNDS DETERMINE THE MOLECULAR FORMULA OF ORGANIC COMPOUNDS
Mass spectrometry detail to up
Mass spectrometry detail to up
Shouvik kumar Nandy
The kidneys lie on the posterior abdominal wall, one on each side of the vertebral column, behind the peritoneum and below the diaphragm The nephron consists of a tubule closed at one end, the other end opening into a collecting tubule Continuing from the glomerular capsule the remainder of the nephron is about 3 cm long and is described in three parts: the proximal convoluted tubule the medullary loop (loop of Henle) the distal convoluted tubule, leading into a collecting duct High efficacy diuretics (Inhibitors of Na-+K+-2Cl¯ cotransport) Sulphamoyl derivatives : Furosemide, Bumetanide, Torasemide 2. Medium efficacy diuretics (Inhibitors of Na+-Cl¯ symport) Benzothiadiazines (thiazides) Hydrochlorothiazide, Benzthiazide, Hydroflumethiazide, Bendroflumethiazide Thiazide like (related heterocyclics) Chlorthalidone, Metolazone, Xipamide, Indapamide, Clopamide 3. Weak or adjunctive diuretics (a) Carbonic anhydrase inhibitors : Acetazolamide (b) Potassium sparing diuretics Aldosterone antagonist: Spironolactone, Eplerenone Inhibitors of renal epithelial Na+ channel: Triamterene, Amiloride. (c) Osmotic diuretics :Mannitol, Isosorbide, Glycerol
Diuretics
Diuretics
Shouvik kumar Nandy
The Mass Spectrometer Quadrupole Mass Spectrometer The compact size and speed of the quadrupole instruments lends them to be efficient and powerful detectors for gas chromatography (GC) Since the compounds are already vaporized, only the carrier gas needs to be eliminated for the process to take place The interface between the GC and MS is shown; a “roughing” pump is used to evacuate the interface
Mass spect. m. pharm
Mass spect. m. pharm
Shouvik kumar Nandy
ROLE OF FREE RADICAL IN NEURODEGENERATIVE DISEASE Oxidative stress in AD?? Oxidative stress occurs when there is an imbalance between t he production and quenching of free radicals from oxygen species. These reactive oxygen species (ROS) play a role in many chronic diseases including mitochondrial diseases, neurodegenerative diseases, renal disease, arteriosclerosis, diabetes , cancer. The process of aging is also associated with increased oxidative stress. Through pathological redox reactions ROS can denature biomolecules such as proteins, lipids and nucleic acids. This can initiate tissue damage via apoptosis and necrosis. Oxidative stress plays a central role in the pathogenesis of AD leading to neuronal dysfunction and cell death. Peripheral markers of oxidative stress are elevated in AD indicating that the damage is not brain-limited. The increased level of oxidative stress in the AD brain is reflected by increased protein and DNA oxidation, Decreased level of cytochrome C oxidase and advanced glycosylation end products. enhanced lipid peroxidation, Lipid peroxidation can weaken cell membranes causes ion imbalance and impair metabolism. Oxidative stress can influence DNA methylation which regulates gene expression. Internalized beta-amyloid may play a role in this process. Mitochondrial dysfunction, which is associated with an accumulation of ROS, appears to play a role in the early events of AD pathology.
Role of free radical in neurodegenerative disease
Role of free radical in neurodegenerative disease
Shouvik kumar Nandy
Free radicals are unstable, highly reactive molecules which are generated both in the body and outside the body. They are described as electron hungry molecules produced when oxygen is metabolized or burned by the body Role of Free Radical in diseases
Generation and role of free radicals 11
Generation and role of free radicals 11
Shouvik kumar Nandy
Responsible conduct of research . • The responsible conduct of research (RCR) involves the following major components: values; policies; planning and conducting research; reviewing and reporting research; and responsible authorship and publication • Institutions conducting research must establish a research office within their institution to facilitate research, manage grants, and oversee all aspects of RCR • The research office must work closely with the EC and with all stakeholders, including undergraduate and postgraduate students • SOPs required to address components of RCR as follows –Values of research - RCR is guided by shared values including honesty, accuracy, efficiency, fairness, objectivity, reliability, accountability, transparency, personal integrity, and knowledge of current best practices, and these should be reflected in RCR-related policies –Policies - Protection of human participants, animal experimentation –Planning and conducting research - specific issues - Conflict of interest; data acquisition, management, sharing and ownership –Reviewing and reporting research –Responsible authorship and publication –Research misconduct and policies for handling misconduct –Registration with Clinical Trials Registry- India –Collaborative research Ethical review procedures Ethical review procedures • Terms of reference for ethics committees (EC) –TOR for the EC and its members should be clearly specified by the institution in the EC SOPs • Special situations –Institutions can have one or more than one EC; each EC can function as a stand-alone committee which should follow all the SOPs and TORs of that institution –An institution that does not have its own EC (user institution) may utilize the services of the EC of another institution (host institution) preferably in the adjoining/nearby area • List of SOPs • Review of multicentric research • Continuing review • Site monitoring • Record keeping and archiving • Administration and management • Registration and accreditation of ECs • Criteria for selection of EC members • Composition of an EC
Icmr guidelines
Icmr guidelines
Shouvik kumar Nandy
DETAIL ABOUT PHARMACOVIGILANCE TOPIC SCHEDULE Y. DEFINITION, DETAIL, RULES AND REGULATIONS easy to study about it. clinical trials in schedule. Salient Features of Schedule Y
Schedule y
Schedule y
Shouvik kumar Nandy
ASTHMA AND COPD FULL DETAIL IN EASY PROCESS, chronic obstructive pulmonary disease. DEFINATION AND REASONS, FACTORS OF IT, INTRINSIC AND EXTRINSIC FACTORS
Copd drugs
Copd drugs
Shouvik kumar Nandy
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Molecular weight can be obtained from a very small sample. It does not involve the absorption or emission of light. A beam of high-energy electrons breaks the molecule apart. The masses of the fragments and their relative abundance reveal information about the structure of the molecule DETERMINE THE MOLECULAR MASS OF ORGANIC COMPOUNDS DETERMINE THE MOLECULAR FORMULA OF ORGANIC COMPOUNDS
Mass spectrometry detail to up
Mass spectrometry detail to up
Shouvik kumar Nandy
The kidneys lie on the posterior abdominal wall, one on each side of the vertebral column, behind the peritoneum and below the diaphragm The nephron consists of a tubule closed at one end, the other end opening into a collecting tubule Continuing from the glomerular capsule the remainder of the nephron is about 3 cm long and is described in three parts: the proximal convoluted tubule the medullary loop (loop of Henle) the distal convoluted tubule, leading into a collecting duct High efficacy diuretics (Inhibitors of Na-+K+-2Cl¯ cotransport) Sulphamoyl derivatives : Furosemide, Bumetanide, Torasemide 2. Medium efficacy diuretics (Inhibitors of Na+-Cl¯ symport) Benzothiadiazines (thiazides) Hydrochlorothiazide, Benzthiazide, Hydroflumethiazide, Bendroflumethiazide Thiazide like (related heterocyclics) Chlorthalidone, Metolazone, Xipamide, Indapamide, Clopamide 3. Weak or adjunctive diuretics (a) Carbonic anhydrase inhibitors : Acetazolamide (b) Potassium sparing diuretics Aldosterone antagonist: Spironolactone, Eplerenone Inhibitors of renal epithelial Na+ channel: Triamterene, Amiloride. (c) Osmotic diuretics :Mannitol, Isosorbide, Glycerol
Diuretics
Diuretics
Shouvik kumar Nandy
The Mass Spectrometer Quadrupole Mass Spectrometer The compact size and speed of the quadrupole instruments lends them to be efficient and powerful detectors for gas chromatography (GC) Since the compounds are already vaporized, only the carrier gas needs to be eliminated for the process to take place The interface between the GC and MS is shown; a “roughing” pump is used to evacuate the interface
Mass spect. m. pharm
Mass spect. m. pharm
Shouvik kumar Nandy
ROLE OF FREE RADICAL IN NEURODEGENERATIVE DISEASE Oxidative stress in AD?? Oxidative stress occurs when there is an imbalance between t he production and quenching of free radicals from oxygen species. These reactive oxygen species (ROS) play a role in many chronic diseases including mitochondrial diseases, neurodegenerative diseases, renal disease, arteriosclerosis, diabetes , cancer. The process of aging is also associated with increased oxidative stress. Through pathological redox reactions ROS can denature biomolecules such as proteins, lipids and nucleic acids. This can initiate tissue damage via apoptosis and necrosis. Oxidative stress plays a central role in the pathogenesis of AD leading to neuronal dysfunction and cell death. Peripheral markers of oxidative stress are elevated in AD indicating that the damage is not brain-limited. The increased level of oxidative stress in the AD brain is reflected by increased protein and DNA oxidation, Decreased level of cytochrome C oxidase and advanced glycosylation end products. enhanced lipid peroxidation, Lipid peroxidation can weaken cell membranes causes ion imbalance and impair metabolism. Oxidative stress can influence DNA methylation which regulates gene expression. Internalized beta-amyloid may play a role in this process. Mitochondrial dysfunction, which is associated with an accumulation of ROS, appears to play a role in the early events of AD pathology.
Role of free radical in neurodegenerative disease
Role of free radical in neurodegenerative disease
Shouvik kumar Nandy
Free radicals are unstable, highly reactive molecules which are generated both in the body and outside the body. They are described as electron hungry molecules produced when oxygen is metabolized or burned by the body Role of Free Radical in diseases
Generation and role of free radicals 11
Generation and role of free radicals 11
Shouvik kumar Nandy
Responsible conduct of research . • The responsible conduct of research (RCR) involves the following major components: values; policies; planning and conducting research; reviewing and reporting research; and responsible authorship and publication • Institutions conducting research must establish a research office within their institution to facilitate research, manage grants, and oversee all aspects of RCR • The research office must work closely with the EC and with all stakeholders, including undergraduate and postgraduate students • SOPs required to address components of RCR as follows –Values of research - RCR is guided by shared values including honesty, accuracy, efficiency, fairness, objectivity, reliability, accountability, transparency, personal integrity, and knowledge of current best practices, and these should be reflected in RCR-related policies –Policies - Protection of human participants, animal experimentation –Planning and conducting research - specific issues - Conflict of interest; data acquisition, management, sharing and ownership –Reviewing and reporting research –Responsible authorship and publication –Research misconduct and policies for handling misconduct –Registration with Clinical Trials Registry- India –Collaborative research Ethical review procedures Ethical review procedures • Terms of reference for ethics committees (EC) –TOR for the EC and its members should be clearly specified by the institution in the EC SOPs • Special situations –Institutions can have one or more than one EC; each EC can function as a stand-alone committee which should follow all the SOPs and TORs of that institution –An institution that does not have its own EC (user institution) may utilize the services of the EC of another institution (host institution) preferably in the adjoining/nearby area • List of SOPs • Review of multicentric research • Continuing review • Site monitoring • Record keeping and archiving • Administration and management • Registration and accreditation of ECs • Criteria for selection of EC members • Composition of an EC
Icmr guidelines
Icmr guidelines
Shouvik kumar Nandy
DETAIL ABOUT PHARMACOVIGILANCE TOPIC SCHEDULE Y. DEFINITION, DETAIL, RULES AND REGULATIONS easy to study about it. clinical trials in schedule. Salient Features of Schedule Y
Schedule y
Schedule y
Shouvik kumar Nandy
ASTHMA AND COPD FULL DETAIL IN EASY PROCESS, chronic obstructive pulmonary disease. DEFINATION AND REASONS, FACTORS OF IT, INTRINSIC AND EXTRINSIC FACTORS
Copd drugs
Copd drugs
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Progesterone
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Estrogen
Estrogen
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Adrenal hormones
Adrenal hormones
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Nsaids
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