crab.pptx

crab preservation , food preservation , water preservation

Lebensmittel

CRABPRESERVATION
⚫FirstdocumentedbyBoyle(1663).
⚫Firstconceptofspermpreservationby
Lazzarospallanzini(1760).
⚫ Most significantdiscoverywastheuseof
glycerolascryoprotectant byPolgeand
smith(1949)

PRINCIPLE
1. FREEZING
2. STORING
3. THAWING

CONT
..
⚫ Theabilityofglycerol toprotectcellsfromfreezing
injurywasdiscoveredaccidentally
.
⚫ Thesubsequentdevelopmentof
CRABPRESERVATION techniqueshashad ahuge
impactinmanyfields, mostnotablyin
reproductivemedicine.
⚫ OK Lets do it
⚫

⚫ Freezing injuryhasbeenshownto havetwo
components,direct damage fromtheicecrystals
andsecondarydamage causedbytheincreasein
concentrationofsolutesasprogressivelymoreiceis
formed.
⚫ Intracellularfreezingisgenerallylethalbutcanbe
avoided bysufficientlyslow cooling, and under
usualconditionssolutedamagedominates.

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