1. Sudden Death and Sickle Cell Trait: How
Knowing Your Genes Can Save Your Life
Dale Lloyd II, No. 39.
(The Rice Football Webletter)
2006
After running 16 consecutive sprints
of 100 yards each, he collapsed.
He died the next day from acute
exertional rhabdomyolysis (ER)
associated with a genetic condition
called sickle cell trait.
Learning Set 4 : Lesson 1 : Slide 1

2. Blood Cells
Normal Blood Sickle Cell Trait Sickle Cell Disease
Cells Blood Cells Blood Cells
Learning Set 4 : Lesson 1 : Slide 2

3. Sickle Cell Disease
• Interview with sickle cell patient: pain
• View Video on sickle cell anemia
• View video on sickle cell trait
Learning Set 4 : Lesson 1 : Slide 3

4. Hemoglobin
Hemoglobin subunit
Red Blood Cell
Oxygen molecule
o o
Learning Set 4 : Lesson 1 : Slide 4

5. Hemoglobin (transport protein)
oxygen molecule (O2)
Hemoglobin
subunit
Red Blood Cell
Learning Set 4 : Lesson 1 : Slide 5

6. Hemoglobin
Learning Set 4 : Lesson 1 6Slide 6
:

7. Hemoglobin
Learning Set 4 : Lesson 1 7Slide 6
:

8. Hemoglobin
Learning Set 4 : Lesson 1 8Slide 6
:

9. Hemoglobin
Learning Set 4 : Lesson 1 9Slide 6
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10. Hemoglobin
Learning Set 4 : Lesson 1 10
: Slide 6

11. Hemoglobin
Learning Set 4 : Lesson 1 11
: Slide 6

12. Hemoglobin
Learning Set 4 : Lesson 1 12
: Slide 6

13. Hemoglobin
Learning Set 4 : Lesson 1 13
: Slide 6

14. Hemoglobin
Learning Set 4 : Lesson 1 14
: Slide 6

15. Electrophoresis of Sickling and
Non Sickling Hemoglobin
Learning Set 4 : Lesson 2 : Slide 7

16. Electrophoresis of Sickling and
Non Sickling Hemoglobin
Learning Set 4 : Lesson 2 : Slide 8

17. Chromosomal Location of Hemoglobin
Chromosome 11
Learning Set 4 : Lesson 2 : Slide 9

18. Mutation found in Hemoglobin
Learning Set 4 : Lesson 2 : Slide 10

19. Mutation found in Hemoglobin
Learning Set 4 : Lesson 2 : Slide 11

20. “Rules” that amino acids follow
• Charge
– Positive and negative charged amino
Red = positive charge (+)
acids attract each other K, R, H
– Amino acids with the same kind of charge
repel each other Yellow = negative charge
• Hydrophobicity (-)
– Hydrophilic amino acids attract water
– Hydrophobic amino acids repel water and
D, E
hydrophilic amino acids Green = hydrophobic
– Hydrophobic amino acids attract other
hydrophobic amino acids. A, V, L, I, P, M, F, W
• Cysteines Blue = hydrophilic
– Cysteines are a type of amino acid. When
two Cysteines are next to each other they G, S, T, N, Q, Y
attract and form a strong bond. These
strong bonds contribute to protein shape
White = cystine
formation. C
Learning Set 4 : Lesson 2 : Slide 12

21. Sickle Cell Hemoglobins Stick Together
Learning Set 4 : Lesson 2 : Slide 13

22. Electrophoresis of Sickling and
Non Sickling Hemoglobin
Learning Set 4 : Lesson 2 : Slide 14

23. Effects of Sickle Cell Mutation
DNA
order of
L T P E E K S L T P V E K S
amino acids
protein
shape
cell
function
Learning Set 4 : Lesson 2 : Slide 14

24. Passing on Mutations
Learning Set 4 : Lesson 3 : Slide 16

25. Frequency of Sickle Cell Causing Mutation
vs. Frequency of Malaria Causing Microbe
http://www.pbs.org/wgbh/evolution/library/01/2/l_012_02.html
Learning Set 4 : Lesson 4 : Slide 17

26. Analyzing RNA Using Gel Electrophoresis
Learning Set 4 : Lesson 5 : Slide 18

27. Who has lactose intolerance in Jason’s family?
Learning Set 4 : Lesson 5 : Slide 19

28. DNA Sequence Analysis of Lactase
Protein Gene
Learning Set 4 : Lesson 5 : Slide 20

29. Gel Electrophoresis: Lactase Protein
Samples from Jason’s Family
Learning Set 4 : Lesson 5 : Slide 21

30. RNA Analysis of Lactase Protein Gene
Learning Set 4 : Lesson 5 : Slide 22

31. DNA Sequence of Area Near the Lactase Gene
Learning Set 4 : Lesson 5 : Slide 23