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Substances pass through the cell
        membrane by:

   1.DIFFUSION
   2.OSMOSIS
   3.ACTIVE TRANSPORT
To remain alive, a cell needs:

to take in the gas:
to eliminate the gas:



    WHY?
                            WHY?
To remain alive, a cell needs FOOD:

Name TWO examples


   GLUCOSE
  AMINO ACIDS


 Name the material which also enters a
  cell & is used as a SOLVENT.
                                water
Salts are also needed

example in plant cells:


    Nitrates
By which process do these materials enter
             or leave a cell?
  Gases by:     DIFFUSION
  Water by:     OSMOSIS
  Glucose
  Amino acids    ACTIVE
  Salts
                 TRANSPORT
Active or Passive transport?




- No energy is needed
The cell membrane is semi-permeable /
    selectively permeable. Explain.
 Outside a cell




     Inside a cell




                      CELL MEMBRANE
The cell membrane allows only certain
     substances to pass through
Outside a cell



                            Porous membrane

    Inside a cell




                                 Only small
                                molecules pass
Is the movement of molecules from a
region of high concentration to a region
         of lower concentration
Liquids & Gases diffuse
Draw arrows to show direction of
            molecules:
Concentration
gradient for
red molecules


Concentration
gradient for
blue molecules
Fig. 1 Diffusion in a liquid.
Why does oxygen keep on entering an
            amoeba?




                      Due to a
                    concentration
                      gradient.
Examples of ‘where’ diffusion occurs:
  Diffusion of CO2 into stomata of leaves
   during photosynthesis.
Diffusion of:
 O2 into the alveoli of the lungs
 CO2 out of capillaries.
The rate of diffusion depends on:
1. Concentration
    the higher the concentration, the faster is
     the rate of diffusion
The rate of diffusion depends on:
2. Temperature
   the higher the temperature, the faster is
     the rate of diffusion
The rate of diffusion depends on:
3. Size of molecules
     the smaller the size, the faster is the rate
       of diffusion
Is the diffusion of water across a semi-
  permeable membrane from a weak
      solution to a strong solution.



    Strong                   Weak
   solution                 solution
Two conditions for osmosis to occur:

              1. A semi-permeable membrane.



Dilute salt                                       Strong salt
 or sugar                                          or sugar
 solution                                          solution




              2. A difference in concentration.
Red blood cells are placed in solutions
  of a different sugar concentration.
           Explain the result.
           Sugar
          molecule


    Let’s
   explain
    each:
RBC & and external solution are at the
        SAME concentration
 1. Water entering RBC =
   water leaving.



    What
   happens?
   No change in RBC size.
RBC are placed in a very concentrated
           sugar solution
2. RBC are dilute
  compared to external
  solution & lose water.


     What
    happens?
      RBC shrink.
RBC are placed in a dilute sugar solution

 3. RBC take up water .



      What
   happens?
   RBC swell and
    finally burst.
Osmosis in plant cells
In a weak solution       In a concentrated
water will enter         solution the cell
the cell and fill the    loses water and
vacuole. The cell        goes flaccid
membrane will            because the
push against the         vacuole becomes
cell wall making         flaccid and the
the cell very            cytoplasm stops
inflexible. It is said   pushing against
that cells in this       the cell wall. This
state are turgid.        state is called
                         plasmolysis.
                                           4.9
What happens to PLANT cells?




Cell in dilute    Cell in same       Cell in     Cell in a much
  solution                       concentrated    concentrated
 becomes:        concentration      solution        solution
                  of solution.     becomes:        becomes:
 TURGID
                                 FLACCID        PLASMOLYSED
Summary: What happens to cells when
   placed in a solution which is :
Explain why red blood cells burst in
distilled water but plant cells do not.




    Rigid cell wall in plants prevents
                bursting.
What happens to the cytoplasm in a
           plasmolysed cell?
It is pulled away from the cell wall.



                          A plant wilts when
                         cells are plasmolysed.
                           Is the plant dead in
                             this wilted sate?
Lose
         water



         Gains
         water




TURGID           PLASMOLYSED
TURGID   PLASMOLYSED
 CELL        CELL
Stomata are:
 OPEN when          CLOSED when
guard cells are:   guard cells are:




                         FLACCID
TURGID
Soil became flooded with seawater. The plant wilted.
However, the plant recovered after it rained. Explain.

        Wilted:                    Plant recovered:
    cells lost water             cells absorbed water
      by osmosis.                     by osmosis.
To demonstrate osmosis in non-living
               material.


Visking tubing / dialysis
tubing / cellulose tubing
To demonstrate osmosis in living tissue.
What do you expect to happen?

Strong sucrose                    Distilled water
solution in bag.                  in beaker.




                   Bag membrane

    Water enters bag by osmosis.
Explain the result of this experiment:

 1. Starch is placed inside a bag.

 2. The bag is put in a tube
 containing iodine solution and
 left for one hour.
 Iodine molecules diffused into
 the bag.
 Bag is selectively permeable.
Explain the results of this experiment

                                                          Salty water

                                    Distilled water
1. Slice your potato into
   5 mm slices.                      2. Place one slice in distilled
                                        water and one in salty water.

Distilled
                                             3. After 30 minutes, you
 water
                                               can bend the slice of
                                               potato in salty water
                                               easily.
                            Salty
                            water
Two pieces of celery were for left for 4
          hours as shown:
        Distilled                 Salty
         water                    water

                    Explain the
                      result.
What has happened?
           Pins mark level of water.




Set up apparatus.            Result after 1 hour.
Why does water enter an amoeba?
 An amoeba is more concentrated than the
surrounding water. Water enters by osmosis.




H2O
Why doesn’t an amoeba burst like a RBC?

      Contractile vacuole releases excess water.
                                 2 Excess water enters
            1 Water enters          contractile vacuole.
               due to osmosis.



  7     The cycle is
         repeated.                                          3
                                                       Contractile
                                                     vacuole swells.

  6 Contractile vacuole
      bursts and expels water.          4     Contractile vacuole
                                             moves to edge of cell.
Salting: Is a way to
  preserve meat.
Explain why there is no need to place jam
            in a refrigerator.

       Bacteria DIE


                            Dilute


  Bacteria die as they
lose water by osmosis.   Concentrated
Have a sore throat? Use a salt gargle.
  WHY is this effective against
           bacteria?




                           Bacteria die as they
                         lose water by osmosis.
Name the process:
 Oxygen moves out from a
  stoma:   diffusion
 Water enters a root hair /
  amoeba:   osmosis
Name the process:

 Glucose absorbed by small
           active transport
  intestine:
Is when substances cross the cell membrane
against a concentration gradient, using
energy and carriers in the membrane.
Large molecules and ions pass through
    proteins in the cell membrane




           proteins
ATP: Adenosine Triphosphate
  ATP is the molecule that supplies
              ENERGY
Fig. 4 Active transport.

                      AGAINST
                   a concentration
                       gradient
How do Osmosis & Diffusion occur?

                 DOWN
         a concentration gradient
                                    Low
 High                               conc.
 conc.


                AGAINST
         a concentration gradient
Question: [MAY, 2009] Paper 2
Paramecium lives in stagnant water. During an
investigation about the average time taken by a
contractile vacuole to fill up and empty, it was
found that the contractile vacuoles fill up and
empty every 60 seconds when placed in water
compared to 180 seconds when placed in 0.3% salt
concentration. Explain. (4)
Question: [MAY, 2009] Paper 2
More water enters a Paramecium living in
water than when is a salt solution. Water
enters the cell by osmosis from a dilute to a
more concentrated salt solution. The more
concentrated the external solution is, the less
water that enters.
Question: [APRIL, 2010] Paper 2

Explain ONE benefit of each of the following
features in the parasitic mode of life of a
parasite living in the gut of its host:

a) body surface covered with microvilli; (1)
  To increase the surface area for absorption of
  food
Question: [APRIL, 2010] Paper 2

b) long and thin body; (1)
  For a large surface area for absorption of food
  (Accept allows food to flow past so that the
  parasite is not washed away/decrease in
  diffusion distance)

c) body wall has active transport systems. (1)
  For uptake of food molecules even against a
  concentration gradient.
Question: [APRIL, 2010] Paper 1
Fresh cherries are fleshy and juicy. Candied
cherries are prepared by placing fresh cherries in a
very strong sugar solution. Explain why candied
cherries lose their fleshy juicy texture. (2)
Question: [APRIL, 2010] Paper 1
Water moves out of the cherries by osmosis.
Cherry cells are dilute when compared to the
surrounding solution. Water follows its
concentration gradient. Cells become plasmolysed.
Cell Membrane Transport

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Cell Membrane Transport

  • 1.
  • 2. Substances pass through the cell membrane by: 1.DIFFUSION 2.OSMOSIS 3.ACTIVE TRANSPORT
  • 3. To remain alive, a cell needs: to take in the gas: to eliminate the gas: WHY? WHY?
  • 4. To remain alive, a cell needs FOOD: Name TWO examples GLUCOSE AMINO ACIDS  Name the material which also enters a cell & is used as a SOLVENT. water
  • 5. Salts are also needed example in plant cells: Nitrates
  • 6. By which process do these materials enter or leave a cell? Gases by: DIFFUSION Water by: OSMOSIS Glucose Amino acids ACTIVE Salts TRANSPORT
  • 7. Active or Passive transport? - No energy is needed
  • 8. The cell membrane is semi-permeable / selectively permeable. Explain. Outside a cell Inside a cell CELL MEMBRANE
  • 9. The cell membrane allows only certain substances to pass through Outside a cell Porous membrane Inside a cell Only small molecules pass
  • 10. Is the movement of molecules from a region of high concentration to a region of lower concentration
  • 11. Liquids & Gases diffuse
  • 12. Draw arrows to show direction of molecules: Concentration gradient for red molecules Concentration gradient for blue molecules
  • 13. Fig. 1 Diffusion in a liquid.
  • 14. Why does oxygen keep on entering an amoeba? Due to a concentration gradient.
  • 15. Examples of ‘where’ diffusion occurs:  Diffusion of CO2 into stomata of leaves during photosynthesis.
  • 16. Diffusion of:  O2 into the alveoli of the lungs  CO2 out of capillaries.
  • 17. The rate of diffusion depends on: 1. Concentration  the higher the concentration, the faster is the rate of diffusion
  • 18. The rate of diffusion depends on: 2. Temperature  the higher the temperature, the faster is the rate of diffusion
  • 19. The rate of diffusion depends on: 3. Size of molecules  the smaller the size, the faster is the rate of diffusion
  • 20. Is the diffusion of water across a semi- permeable membrane from a weak solution to a strong solution. Strong Weak solution solution
  • 21. Two conditions for osmosis to occur: 1. A semi-permeable membrane. Dilute salt Strong salt or sugar or sugar solution solution 2. A difference in concentration.
  • 22. Red blood cells are placed in solutions of a different sugar concentration. Explain the result. Sugar molecule Let’s explain each:
  • 23. RBC & and external solution are at the SAME concentration 1. Water entering RBC = water leaving. What happens? No change in RBC size.
  • 24. RBC are placed in a very concentrated sugar solution 2. RBC are dilute compared to external solution & lose water. What happens? RBC shrink.
  • 25. RBC are placed in a dilute sugar solution 3. RBC take up water . What happens? RBC swell and finally burst.
  • 26. Osmosis in plant cells In a weak solution In a concentrated water will enter solution the cell the cell and fill the loses water and vacuole. The cell goes flaccid membrane will because the push against the vacuole becomes cell wall making flaccid and the the cell very cytoplasm stops inflexible. It is said pushing against that cells in this the cell wall. This state are turgid. state is called plasmolysis. 4.9
  • 27. What happens to PLANT cells? Cell in dilute Cell in same Cell in Cell in a much solution concentrated concentrated becomes: concentration solution solution of solution. becomes: becomes: TURGID FLACCID PLASMOLYSED
  • 28. Summary: What happens to cells when placed in a solution which is :
  • 29. Explain why red blood cells burst in distilled water but plant cells do not. Rigid cell wall in plants prevents bursting.
  • 30. What happens to the cytoplasm in a plasmolysed cell? It is pulled away from the cell wall. A plant wilts when cells are plasmolysed. Is the plant dead in this wilted sate?
  • 31. Lose water Gains water TURGID PLASMOLYSED
  • 32. TURGID PLASMOLYSED CELL CELL
  • 33. Stomata are: OPEN when CLOSED when guard cells are: guard cells are: FLACCID TURGID
  • 34. Soil became flooded with seawater. The plant wilted. However, the plant recovered after it rained. Explain. Wilted: Plant recovered: cells lost water cells absorbed water by osmosis. by osmosis.
  • 35.
  • 36. To demonstrate osmosis in non-living material. Visking tubing / dialysis tubing / cellulose tubing
  • 37. To demonstrate osmosis in living tissue.
  • 38. What do you expect to happen? Strong sucrose Distilled water solution in bag. in beaker. Bag membrane Water enters bag by osmosis.
  • 39. Explain the result of this experiment: 1. Starch is placed inside a bag. 2. The bag is put in a tube containing iodine solution and left for one hour. Iodine molecules diffused into the bag. Bag is selectively permeable.
  • 40. Explain the results of this experiment Salty water Distilled water 1. Slice your potato into 5 mm slices. 2. Place one slice in distilled water and one in salty water. Distilled 3. After 30 minutes, you water can bend the slice of potato in salty water easily. Salty water
  • 41. Two pieces of celery were for left for 4 hours as shown: Distilled Salty water water Explain the result.
  • 42. What has happened? Pins mark level of water. Set up apparatus. Result after 1 hour.
  • 43. Why does water enter an amoeba? An amoeba is more concentrated than the surrounding water. Water enters by osmosis. H2O
  • 44. Why doesn’t an amoeba burst like a RBC? Contractile vacuole releases excess water. 2 Excess water enters 1 Water enters contractile vacuole. due to osmosis. 7 The cycle is repeated. 3 Contractile vacuole swells. 6 Contractile vacuole bursts and expels water. 4 Contractile vacuole moves to edge of cell.
  • 45. Salting: Is a way to preserve meat.
  • 46. Explain why there is no need to place jam in a refrigerator. Bacteria DIE Dilute Bacteria die as they lose water by osmosis. Concentrated
  • 47. Have a sore throat? Use a salt gargle. WHY is this effective against bacteria? Bacteria die as they lose water by osmosis.
  • 48. Name the process:  Oxygen moves out from a stoma: diffusion  Water enters a root hair / amoeba: osmosis
  • 49. Name the process:  Glucose absorbed by small active transport intestine:
  • 50. Is when substances cross the cell membrane against a concentration gradient, using energy and carriers in the membrane.
  • 51. Large molecules and ions pass through proteins in the cell membrane proteins
  • 52. ATP: Adenosine Triphosphate ATP is the molecule that supplies ENERGY
  • 53. Fig. 4 Active transport. AGAINST a concentration gradient
  • 54. How do Osmosis & Diffusion occur? DOWN a concentration gradient Low High conc. conc. AGAINST a concentration gradient
  • 55. Question: [MAY, 2009] Paper 2 Paramecium lives in stagnant water. During an investigation about the average time taken by a contractile vacuole to fill up and empty, it was found that the contractile vacuoles fill up and empty every 60 seconds when placed in water compared to 180 seconds when placed in 0.3% salt concentration. Explain. (4)
  • 56. Question: [MAY, 2009] Paper 2 More water enters a Paramecium living in water than when is a salt solution. Water enters the cell by osmosis from a dilute to a more concentrated salt solution. The more concentrated the external solution is, the less water that enters.
  • 57. Question: [APRIL, 2010] Paper 2 Explain ONE benefit of each of the following features in the parasitic mode of life of a parasite living in the gut of its host: a) body surface covered with microvilli; (1) To increase the surface area for absorption of food
  • 58. Question: [APRIL, 2010] Paper 2 b) long and thin body; (1) For a large surface area for absorption of food (Accept allows food to flow past so that the parasite is not washed away/decrease in diffusion distance) c) body wall has active transport systems. (1) For uptake of food molecules even against a concentration gradient.
  • 59. Question: [APRIL, 2010] Paper 1 Fresh cherries are fleshy and juicy. Candied cherries are prepared by placing fresh cherries in a very strong sugar solution. Explain why candied cherries lose their fleshy juicy texture. (2)
  • 60. Question: [APRIL, 2010] Paper 1 Water moves out of the cherries by osmosis. Cherry cells are dilute when compared to the surrounding solution. Water follows its concentration gradient. Cells become plasmolysed.