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Ravindra Paidisetti
Flat 122, Trident Grande Apartments,Survey # 101,Gundlapochampalli, Hyderabad-14, Phone: 9652070232;8790533411
Email: rkpaidisetti@gmail.com
Professional with more than six years of industrial experience in research and development with skill sets of microbiology, molecular biology
and biochemistry. Being a committed player I want to be a part of a renowned organization and contribute towards growth of the organization
through my expertise. At the same time further my personal capabilities from the new exposure within the structured framework of the
organization.
o Worked on top priority projects including, bio-butanol production, industrial application of enzymes, and heterologous flavonoid
(nutraceutical) production.
o Skilled in application of biotechnology tools to engineer microbes for production of high value molecules. Extensive knowledge in
molecular biology, enzyme assays and high throughput screening.
o Skilled in strategic designing, execution, data analysis and troubleshooting of experiments.
Industrial experience
Researchassociate (level2), Industrial Biosciences(IBT) Nov 2014to date
E.I. DuPont India Pvt Ltd, DuPont knowledge center, Hyderabad.
o Constructed heterologous pathway in yeast to produce flavonoids (Manuscript for publication is under process).
o Standardized, validated and carried out enzymes assays (in vitro) to screen individual flavonoid, butanol, and glycolytic pathway
enzymes.
o Optimized enzyme assays (in vitro) for high throughput screening and provided support to fermentation team for various projects.
o Established quantitative PCR (qPCR) studies (both 96& 384 well plates) in the lab and evaluated expression profiles of genes at the
transcription level for flavonoid and stress response pathway in yeast strains.
o Identified and tested the efficacy of biologics samples.
Researchassociate (level1), Central research & development June 2009to Nov 2014
E.I. DuPont India Pvt Ltd, DuPont knowledge center, Hyderabad.
o Developed a novel application of enzymes in bio-refineries segment to mitigate contamination in processes-fermentation, shake flask
evaluations. A project to find alternatives to usage of antibiotics in fermentation (patent filing is in progress).
o Generated industrial strains with impaired sporulation through gene deletion by scar-less method. Studied eukaryotic promoter
strengths and identified suitable integration sites in industrial yeast strains.
o Generated mutant libraries of industrial strains with desired characteristics. Carried out cell-metabolite toxicity studies.
Non technical:
o Procured chemicals and common consumables for lab and maintained inventory.
o Responsible for the safety and maintenance of critical lab areas
Academicexperience
Graduate research and teaching assistant University of Idaho, Moscow, ID. Jan ‘04 - Aug ‘07
o Developed “time course lysis”, a novel method to extract and identify co-metabolic protein biomarkers from complex microbial, an
interdisciplinary project supported by the Department of Energy (DOE).
o Developed a method for extracting high-molecular-weight DNA, constructed 16S rDNA libraries, performed statistical analysis and
studied phylogenetic relationships in a project supported by National Institute of Health (NIH).
o Maintained cell cultures, submitted reports with project results, performed literature/library searches and ordered necessary lab
equipments and supplies. Organized and administered pre-lab instruction and guidance for introductory biochemistry lab.

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Technical Skills
Microbiology:
o Growth, maintenance and preservation of bacteria and yeast cultures, shake-flask fermentation procedures and growth studies.
Staining, cell counting and morphological observations. Identification of yeast mating types and switching of mating types to
generate diploids from haploids. Yeast random sporulation and generation of haploids from diploids. Light, fluorescent and confocal
microscopy.
Molecular biology:
o Traditional cloning, cloning by in vivo ligation (through gap repair in yeast), transformation and library construction, genomic
sequence data mining and analysis, UV spectrophotometry of nucleic acids, site-specific gene integration, gene deletion by
disruption and scare-less gene deletion, genomic and plasmid DNA extraction and purification, PCR, RNA extraction and
purification, quantitative PCR (qPCR), restriction digestion, Restriction Fragment Length Polymorphism (RFLP) and Denaturing
Gradient Gel Electrophoresis (DGGE)
Biochemistry
o Total protein extraction and purification methods, mitochondrial extraction and purification, protein quantification assays, protein
thermal stability assays. Standardization of enzyme assays (In Vitro) and kinetic analysis and high-throughput enzyme assays. SDS
PAGE and agarose gel electrophoresis and staining techniques, UV spectrophotometry of proteins.
Education
o Master of Science in Microbiology, MolecularBiology and Biochemistry (GPA 3.4/4.0) Dec‘07
Universityof Idaho, Moscow, ID,USA.
Thesis: “Ecoproteomic and Ecogenomic Approaches to Monitor Microbial Activity in Subsurface Aquifer and Effect of Compost
Infusion Enrichment on Bacterial Community Structure of Polycyclic Aromatic Hydrocarbon Contaminated Soils”.
o Master of Science in Human Nutrition (Non thesis) (GPA 3.6/4.0) Jul ‘03
Universityof Bridgeport, Bridgeport, CT,USA.
o Master of Science in Biochemistry (Non thesis) May‘00
Nagarjuna University,India.
o Bachelor of Science in Biochemistry, Chemistry and Zoology May‘98
Andhra University, India.
Other skills
Computer skills: Bioinformatics tools & statistical softwares: (a) Vector NTI (b) ClustalX (c) DOTUR (d) PAUP (e) BLAST (f) BLASTp (g)
Sigma plot (h) SAS® software (SAS/BASE, SAS/STAT, SAS/SQL, SAS/MACRO & SAS/GRAPH) SAS® certification: Certified as Base
Programmer for SAS® 9.
Safety
Trained in lab, equipment safety procedures and writing and maintaining of process safety management (PSM) for experimental protocols and
standard operating protocols (SOPs)
Interpersonal skills
Effective communication skills and ability to work collaboratively in a team and capable of working independently when required
Publication
Proteomic and targeted qPCR analyses of subsurface microbial communities for presence of methane monooxygenase. Paszczynski, Andrzej
J.; Paidisetti, Ravindra; Johnson, Andrew K.; Crawford, Ronald L.; Colwell, Frederick S.; Green, Tonia; Delwiche, Mark; Lee, Hope3; Newby,
Deborah; Brodie, Eoin L.; Conrad, Mark. Biodegradation, Volume 22, issue 6 (November 2011), p. 1045 - 1059. ISSN: 0923-9820 DOI:
10.1007/s10532-011-9462-4. Springer Netherlands, Dordrecht

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Presentations
1) Paidisetti, R., Johnson, A., Crawford, R., and Paszyznski, A. Using Proteomics to Sense Natural Attenuation in the INL Test Area North
(TAN) Aquifer. Environmental sensing symposium, Boise State University, Boise, October 25-26. pp. 102.
2) Paidisetti, R., Crawford, R., and Paszyznski, A. Extracting and identifying protein biomarkers from TCE-contaminated groundwater.
Environmental and Subsurface Science Symposium, University of Idaho, Moscow, September 25-27, 2006. pp.18.
3) Paidisetti, R., Strap, J. L, Colwell, F., Crawford, R., and Paszyznski, A. Identification of proteins from Methylosinustrichosporium OB3b
responsible for methane oxidation using proteomic tools. Subsurface Biotechnology and Bioremediation Symposium and Workshop.
Montana State University, Bozeman, June 22-23, 2006.
4) Paidisetti, R., Fusco, W., Jung, C., Paszczynski, A., Colwell, F., and Crawford, R. Purification and characterization of metagenomic DNA
from hydrocarbon-contaminated Soils. American Society for Microbiology, 105th General Meeting, Atlanta, June 5-10, 2005. Q-073.
5) Paidisetti, R., Fusco, W., Jung, C., Brinkman, C., Kim, K., Paszczynski, A., Colwell, F., and Crawford, R. Obtaining of high-molecular
weight DNA from contaminated soils. Superfund Basic Research Program Annual Meeting. University of Washington, Seattle, November
3-4, 2004. pp. 30.
Honors & activities
1) Awarded “Night in the Town” for contributions in non technical support to IBT group. DuPont knowledge center; Dec 2015
2) Awarded “Night in the Town” for enzymatic assay support to the fermentation team. DuPont knowledge center; Oct 2012
3) Third place in the student poster presentation competition, Inland Northwest Research Alliance (INRA) Environmental Sensing
symposium, Bose State University, Boise, ID, October 25-26, 2007.
4) Second place in the student poster presentation competition, Inland Northwest Research Alliance (INRA) Environmental & Subsurface
Science symposium, University of Idaho, Moscow, ID, September 25-27, 2006.
5) Corporate Activity Student Travel Grant Award, American Society for Microbiology (ASM) 105th General Meeting, Atlanta, June 5-10,
2005.
References
Available upon request.