1. 1-я Международная конференция "Модели инновационного развития фармацевтической и медицинской промышленности на базе интеграции университетской науки и индустрии" Фотоконтроль и конструирование сердечной ткани К.И. Агладзе

2. Стратегия работы Фото-контроль сердечной ткани Фото-контролируемая сконструированная человеческая сердечная ткань Сконструированная сердечная ткань на основе нановолокон Сердечная ткань, полученная из плюрипотентных клеток

3. Photo-controlled cardiac tissue

4. N+ O N N O N O N+ N+ O N O N S OH N N O O- OH N N+ N HO O O Substances tested (azobenzene derivatives) N O (1) N+ O N (2) (3) (4) (5) Spontaneous Activity after Washout Suppression of Excitation UV/Vis Response Range (1) (2) (3) (4) (5) 0 – 1.0 mM 0 – 0.2 mM 0 – 0.5 mM 0 – 1.0 mM 0 – 0.3 mM

5. Light induces cis-trans or trans-cisisomerization of AC Does not block channels Blocks channels Activation Inhibition trans-form cis-form UV (365 nm) Blue (440 nm)

6. Reversible suppression of excitation waves in cardiomyocyte culture <Experimental Setup> UV+BLUE BLUE (490 nm) 2 mW <The Movie> UV (365 nm) 4 mW UV-cutoff filter UV Cardiomyocytes Propagation speed vs AC concentration UV + BLUE Upper = Blue Lower = Blue & UV Wave Speed / mm s-1 BLUE The shield was removed in a course of experiment (Speed: 2X) [Azo-compound] / mM

7. (BLUE) (UV) Patterning (BLUE) 0.2s 0.0s 0.4s 0.6s 1.2s 2.8s 3.8s 0.8s (UV) (Fluorescence Intensity) (Speed: 1X) 10 mm Artificial Pacemaker 10 mm (Speed: 2X) 10 mm (time interval = 0.2 sec)

8. 10 sec Reversible Suppression of Excitation in a Whole Heart (Langendorf preparation of mouse heart) Excitation Monitoring in a Whole Heart Preparation <<Fluorescence image produced by membrane-potential sensitive dye>> (Time interval = 0.1 sec) <Control> Intensity / a.u. <WITH Azo-compound> Measured Point (Speed: 1X(looped) )

9. Effect of AzoTab on action potential formation in rat neonatal myocytes 60 40 control 20 AzoTab 0.5 mM (after 6 min.) AzoTab 0.5 mM ( after 8 min.) 0 Membrane potential, mV AzoTab 0.5 mM + UV -20 -40 -60 -80 0 200 400 600 800 1000 Time, ms

10. 150 100 50 0 20 sec 20 sec Specific versus non-specific binding Switch between UV – Blue light 100 Counts / a.u. Speed / mm s-1 (Addition of AzoTAB) 50 Addition and washout data Laser Raman spectrometer: Nanofinder 30 Laser: 532 nm Brown: 0.5 mM AzoTAB solution of Tyrode Blue: (1) Exchange medium to 0.5 mM AzoTAB solution of Tyrode (2) Exposure blue light (4 mW, 60 sec) (3) Rinsing in new Tyrode 3 times under blue light (4) Dried up Violet: (1) Exchange medium to 0.5 mM AzoTAB solution of Tyrode (2) Exposure blue light (4 mW, 60 sec) (3) Exposure UV light (7 mW, 60 sec) (4) Rinsing in new Tyrode 3 times under UV light (5) Dried up Black: (1) Rinsing in new Tyrode (2) Dried up Wash out 0 Speed / mm s-1 time 1000 1200 1400 1600 1800 2000 Raman Shift / cm-1 time : BLUE (4 mW) : BLUE (4 mW) + UV (6 mW)

11. Insect’s dorsal vessel (Photo) <CtenoplusiaAgnata> [AzoTAB] = approx. 0.2 mM (Insect_100416.wmv) (Dorsal Vessel.wmv) (Movie)

12. Nanofiber-based engineered cardiac tissue

13. Polymer nanofibers as a tool for cardiac tissue engineering Methods: Cells guided by nanofibers on solid substrate Cells guided by substrate-free nanofibers Advantages: Controlled alignment of cells Precise positioning of the cells Porous 3D constructs

14. Fabrication of Polymer Nanofibers by Electrospinning Electrospinning Apparatus Material: 13% concentration solution of PMGI (polymethylglutarimide) in cyclopentanone with adding of ionic surfactant (Sodium dodecyl sulfate, 0.48 g/l) and Rhodaminedye (0.1%) Working parameters: Voltage - 8kV; Flow rate - 1.5-2.0 ml/h; Spraying time - 2-15 seconds depending on desired positioning density of nanofibers; Working distance - 10 cm; Collector – Al foil, 100 µm 6 mm

15. Transferring of nanofibers by micro contact printing PDMS layer with polymer nanofibers as a stamp for microcontactprinting Collector with nanofibers Clean glass substrate PDMS layer cleaned with ethanol Stage 2000C PDMS (polydimethylsiloxane) layers with polymer nanofibers Glass substrate covered with PMGI nanofibers after cooling and separation

16. Cardiac tissue culture being grown on nanofibers-free solid substrate Cardiac tissue culture being grown on solid substrate covered with nanofibers

17. Cardiac tissue culture being grown on solid substrate covered with nanofibers Fibers, Rhodamin Actin, Alexa Fluoro 488 Nuclei, DAPI

18. Functionality of Cardiac Monolayers 2 1 3 5 4 Positions of electrode during stimulation Across fibers – 0.2 sec; Along fibers – 0.36 sec; Ratio – 1.8 Fluo-4 stained 1 2 3 4 5 6 7 8 9 10

19. Functionality of Cardiac Monolayers 2 1 Distance, mm Fibers’ direction Time, s Horizontal direction - along fibers Vertical direction - across fibers

20. Anisotropy of Cardiac Tissue Culture

21. Precise Positioning of the Cells (1) Collagen, Type I from Calf Skin + HFP (Hexafluoro-2-propanol) (2) PMGI+ Fibronectin (3) PMGI+ Collagen Collagen Collagen

22. Precise Positioning of the Cells Single Collagen Fiber Porous Collagen Fiber Net

23. Precise Positioning of the Cells Group of Collagen Fibers Fluo-4 stained

24. Preparation of Polymeric Scaffold for 3D Culture Engineering Cover with fibronectin PDMS Holder with Nanofibers Collector Seeding cells 1 PDMS layer cleaned with ethanol 2 Stage Porous PMGI Fiber Net Single Cell – Single Fibre Interaction

25. 3D Cardiac Tissue Engineering Porous PMGI Fiber Net

26. Cardiac tissue derived from IPS cells

27. Cardiomyocyte layers with contraction and propagating waves Optical mapping Immunostaining Mouse ES derived Human iPS derived α-actinin (cardiac marker) DAPI

28. Konstantin Agladze Lab Biophysics, Non-linear Science Chemical tools to control the ion channel activity • Cell membrane architecture/function and meso-control • Ion channel/transporter/receptor with bio-functional chemicals/materials