1. 3rd Aug 2015
SONAL
(Plant Bio-Technology--Algae)
1
Presentation
on
Industrial Training

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INTRODUCTION
Haematococcus Pluvialis
It is a fresh water species of chlorophyta from family
Haematococcaceae.
It is usually found in temperate region.
It is a green microalgae contain astaxanthin i.e. strong antioxidant.
More than 40g of astaxanthin (Keto-carotenoid) can be obtained
from 1kg of dry biomass.
Astaxanthin produced from Haematococcus Pluvialis is a main
source for human consumption.

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ASTAXANTHIN
• Red fat soluble pigment
• Higher antioxidant properties.
• Used in various drugs, lotions and creams
• Protects brain from nervous damage
• It has anti-cancer, anti diabetic, anti-inflammation activities

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RW1-170P-PHOTOBIOREACTOR

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INOCULATION OF MEDIUM AND DEVELOPMENT OF GROWTH CURVE
Icubated Under Outdoor Direct Sunlight
DAY DATE CULTURE CONDITION O.D pH
0th 09-06-15 CONTROL 0.00 7.02
TEST-1 0.00 7.24
TEST-2 0.00 7.25
9th 18-06-15 CONTROL 0.00 7.95
TEST-1 0.07 7.91
TEST-2 0.08 7.92
15th 24-06-15 CONTROL 0.00 7.90
TEST-1 0.13 7.97
TEST-2 0.16 7.99
Incubated Under Double Tube Light
DAY DATE CULTURE CONDITION O.D pH
0th 12-06-15 CONTROL 0.00 7.40
TEST-1 0.00 7.56
TEST-2 0.00 7.58
6th 18-06-15 CONTROL 0.00 7.97
TEST-1 0.02 7.97
TEST-2 0.03 7.96
14th 26-06-15 CONTROL 0.00 8.06
TEST-1 0.09 8.12
TEST-2 0.08 8.34

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MORPHOLOGICAL IDENTIFICATION OF HAEMATOCOCCUS PLUVIALIS
AT 10X AT 45X AT 100X

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STANDARDIZATION OF DAILY HARVESTED VOLUME FROM
MASS CULTIVATED ALGAE
At 0th day After 19 days

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY NITROGEN SOURCE
DAY DATE CULTURE
CONDITION
O.D. pH
0th 15-06-15 CONTROL 0.00 7.58
FLASK-1(0.5g) 0.00 7.57
FLASK-2(0.5g) 0.00 7.53
FLASK-1(1g) 0.00 7.54
FLASK-2(1g) 0.00 7.55
FLASK-1(1.5g) 0.00 7.54
FLASK-2(1.5g) 0.00 7.54
15th 29-06-15 CONTROL 0.00 6.47
TEST-1 (0.5g) 0.13 6.37
TEST-2(0.5g) 0.10 6.03
TEST-1(1g) 0.07 6.31
TEST-2(1g) 0.08 6.02
TEST-1(1.5g) 0.07 6.21
TEST2(1.5g) 0.06 6.27
AFTER 15 DAYS
FLASKS(0.5g, 1g)
FLASKS(1.5g)

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY VITAMINS
DAY DATE CULTURE
CONDITION
O.D. pH
1st 19-06-15 CONTROL 0.00 8.02
FLASK-1(0.5ml) 0.00 8.06
FLASK-2 (1ml) 0.00 8.03
FLASK-3(1.5ml) 0.00 8.05
11th 29-06-15 CONTROL 0.00 8.11(6.11)
FLASK-1(0.5ml) 0.07 8.79 (6.16)
FLASK-2 (1ml) 0.07 8.80(6.20)
FLASK-3(1.5ml) 0.05 8.17(6.22)
25th 13-07-15 CONTROL 0.00 8.50
FLASK-1(0.5ml) 0.20 9.70
FLASK-2 (1ml) 0.27 9.67
FLASK-3(1.5ml) 0.13 9.00
At 1st day
After 25 days

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY LIGHT
DAY DATE CULTURE
CONDITION
O.D. pH
0th 16-06-15 CONTROL 0.00 6.06
FLASK-1(D.T.L) 0.00 6.07
FLASK-2(S.T.L) 0.00 6.12
FLASK-3(I) 0.00 6.01
FLASK-4(O) 0.00 6.09
9th 25-06-15 CONTROL 0.00 8.03
FLASK-1(D.T.L) 0.07 8.98
FLASK-2(S.T.L) 0.03 8.43
FLASK-3(I) 0.07 8.93
FLASK-4(O) 0.05 9.12
20th 06-07-15 CONTROL 0.00 6.23
FLASK-1(D.T.L) 0.17 6.38
FLASK-2(S.T.L) 0.10 6.26
FLASK-3(I) 0.21 6.19
FLASK-4(O) 0.19 6.41
AFTER 20 DAYS
UNDER DOUBLE TUBE AND
SINGLE TUBE LIGHT
UNDER INDOOR AND
OUTDOOR IN DIRECT SUNLIGHT

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY pH
DAY DATE CULTURE
CONDITION
O.D. pH
0th 22-06-15 CONTROL 0.00 7.28
FLASK-1(pH 7) 0.00 7.34
FLASK-2(pH 8) 0.00 7.32
FLASK-3(pH 9) 0.00 7.34
9th 01-07-15 CONTROL 0.00 7.88
FLASK-1(pH 7) 0.07 7.95
FLASK-2(pH 8) 0.05 8.58
FLASK-3(pH 9) 0.04 8.23
21st 13-07-15 CONTROL 0.00 7.91
FLASK-1(pH 7) 0.19 7.95
FLASK-2(pH 8) 0.22 8.87
FLASK-3(pH 9) 0.14 8.89
AFTER 21 DAYS
GROWTH AFFECTED BY pH

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TRANSESTERIFICATION
SEPARATED BIODIESEL IN A FUNNEL SEPARATED GLYCEROL

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BEFORE PASSING CURRENT AFTER PASSING CURRENT
HARVESTING OF MICROALGAE WITH ELECTROFLOCULATION

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B STRAIN OF ASTAXANTHIN (AHB)
AT 10X AT 45X AT 100X

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ISOLATION OF ALGAL CULTURE BY SKINNER’S TECHNIQUE

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ISOLATION OF ALGAL CULTURE BY SERIAL PLATE METHOD

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MARKET SCOPE OF ASTAXANTHIN
Market Sectors Market Size
(as of 2009) ( Million USD)
Potential Market
(2020) (Million USD)
Animal feed coloring
agents
300 800
Antioxidant
neutraceuticals
30 300
Pharmaceuticals Emerging 500
Cosmetics Emerging 30

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Thanks