Production of insulin from microbial culture

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Muhammad Aklil Ayob

Technologie Gesundheit & Medizin

PRODUCTION OF INSULIN
FROM MICROBIAL
CULTURE
RUI BAI
MUHAMMAD AKLIL BIN AYOB
YANGJIE CUI

CHEN 60351 : CORE
BIOTECHNOLOGY 1

CONTENT
1.
2.
3.
4.
5.

Abstract
Introduction
Main process of insulin production
Significance of human insulin
Conclusion

Abstract







Hormone secreted by
pancreas
Control blood sugar level
Lack of insulin caused
diabetes mellitus
Industrial production by
manipulating genetic
engineering and microbial

Introduction






Protein generated
by beta cell of
pancreas (islet of
Langerhans)
Exist in animal:
porcine and bovine
Build up of 51 amino
acids







30 amino acids form
chain B
21 amino acids form
chain A
2 chain linked by
disulfide bonds

(Introduction)…cont

www.bio.davidson.edu
www.depositphotos.com

(Introduction)…cont




Diabetes mellitus
(DM) lack of insulin
to control blood
sugar level
Need to insulin
therapy to keep
healthy
www.som.uq.edu.au

Main process of insulin production
Microorganisms

Fungi

Yeast

Microorganisms

Kinds of bacteria

E. coli
www.google.com.hk

Significance of human insulin



1978: insulin from animal; pig and cow
Switch to microbial culture. Why?


Quantity & quality is better




E. coli culture is convenient




No allergy and side effect
Cheaper for commercial

Predictable


Problems easily solved

Conclusion








Insulin market grown
at 7% annual rate
Company: Novo
Nordisk, Eli Lilly &
Sanofi Aventis
Cost : £75 – 200 per
vial
Different delivery ways

www.novonordisk.com

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