As Monica mentioned we were getting beautiful blue 911T E. coli pellets.
Color is correct. Other reasons explain the small yield.
Our task was to capture the bacterial population from the blue pellet, grow it in nutrient broth using sterile technique for mini prepping of its plasmid DNA, Finally sequence its plasmid DNA to confirm its identity by aligning it with our known genes.
We are back on track – expressing and characterizing 911 in its truncated GAF only form.
Now Valerie Metea will tell you more about protein purification
![Bioengineering Red Fluorescent Tags from Thermosynechococcus elongatus ,[object Object],[object Object]](https://image.slidesharecdn.com/mobarrettsep2011ver2a-110927113418-phpapp01/85/Mo-s-Barret-Presentation-1-320.jpg)
