1. Interpret Tutorial — Topic 1: Getting Started Dr Ruth Burton, Product Manager — Clinical Solutions

2. Overview In this tutorial we will cover the following topics: PC specifications and installation Step 1: Loading the OGT protocol Step 2: Loading data using the Workflow mode Step 3: Data analysis Step 4: Sample annotation Step 5: Review Step 6: Aberration classification Step 7: Report generation

3. PC specifications CytoSure™ Interpret Software is optimised for use on computers running Windows XP® or Windows Vista® To ensure correct display, do not use Windows classic view. For viewing and saving multiple datasets it is recommended that a computer with a memory of 3–4 GB is used A quad core computer is recommended to ensure fast running of the automated aberration detection function Typical data processing time, when using a CytoSure ISCA 8 x 60k array, should be approximately 4–5 minutes CytoSure Interpret Software works effectively on non-quad core computers; however, the automated aberration detection running time will take longer

4. Installation To install CytoSure Interpret Software from the supplied CD, double-click on the installer file “CytoSure Interpret Software.exe” and follow the on screen instructions If the software does not start installing automatically, the application can be started via the Windows “Start” Menu Click “Start” and in the drop down list select “All Programs” Another drop-down list appears, select “CytoSure Interpret Software” Alternatively double-click the icon on the desktop

6. Step 1: Loading the OGT protocol

7. Step 1: Loading the OGT protocol

8. Step 1: Loading the OGT protocol

9. Step 1: Loading the OGT protocol

10. Step 1: Loading the OGT protocol

11. Step 1: Loading the OGT protocol

12. Step 2: Loading data Click the load icon

13. Step 2: Loading data Select the file type you want to load

14. Step 2: Loading data

15. Step 2: Loading data

16. Step 3: Data analysis Click the process icon

17. Step 3: Data analysis The dialog box shows the progress of analysis

18. Step 4: Sample annotation

19. Step 4: Sample annotation

20. Step 5: Review

21. Step 5: Review

22. Step 5: Review

23. Step 5: Review

24. Step 5: Review

25. Step 5: Review

26. Step 6: Aberration classification

27. Step 6: Aberration classification

28. Step 6: Aberration classification

29. Step 7: Report generation

30. Step 7: Report generation

31. Tip:

32. If you have any questions, please contact products@ogt.co.uk

33. Thank you www.ogt.co.uk