Scott Alexander with Malcolm Crittenden and Robert Ryder, PA Department of Environmental Protection (DEP), “Instream Limestone Sand Dosing in the Allegheny Plateau” Attendees will learn of the successful recovery of trout populations in low-buffered, acidic streams by the use of alkaline treatments. Documented low-cost/long term trout restoration methods such as (ILS) may be useful for other watersheds dealing with stream acidification because several high cost alternatives have been proposed in Pennsylvania which on analysis may deliver far less stream-alkalinity per dollar.

15. Downstream Upstream
The biologically diverse stream segment is
the component of the watershed, that under
the normal range of conditions of consistent
annual flow, alkaline buffering and stabile
habitat, a diverse community of uni /
semivoltine taxa and other macroinvertebrates,
are established and biologically productive,
providing the building blocks of aquatic ecologic
systems.
Diverse community
of long-lived
macroinvertebrates
Biologically Diverse Biologically Variable Upland Spring
Stream Segment Stream Segment and Seeps

20. Isers
Run
(Ideal/
ATI
Ref.)
Cranberry
Run
(Watershed
Reference)
Optimal
Watershed
Reference
(Average
of
Ideal/ATI
Reference
And
Watershed
Reference)
SP7
Each candidate site
will be compared
to:
1. Past to Current
2. “Non-Impaired”
Watershed Reference
3. Optimal
Watershed
Reference
4. Ideal/ATI Reference
SP9 LF10

21. “Tipping Point” for Laurel Falls Run
In the Headwaters, at Site SP7
Date
pH Alk Acidity
(su) (mg/l) (mg/l)
6/21/2007 4.60 6.6 16.6
9/30/2009 4.70 5.0 17.0
12/19/2009 4.90 1.5 7.0
3/22/2010 4.80 1.8 9.0
6/23/2010 4.60 1.5 12.5
10/4/2010 4.50 0.5 13.3
Limestone sand dosing started
6/1/2011 6.30 6.0 5.2
10/15/2011 6.20 13.3 0.3
5/2/2012 6.80 9.3 6.8
12/20/2012 6.90 8.0 8.1
3/14/2013 6.90 8.9 -0.4
Total
PRM 2ND Total
Taxa CRI- CRI- CRI- #
Date Richness ACD ACD ACD Indiv.
(> U/SV)(> U/SV)(>U/SV)
(FATI)(“”, PR)(“”, FFG)
(20) (10) (10)
12/5/2010 7 3 1 2 #15
41 8 15 CRI-ACD
Qual. Bio.
Score
12/8/2011 7 3 1 2 #32
36 8 14 CRI-ACD
QBS
- No major Qualitative change in
Indicators of Com. Reestablishment
compared to Historical Reference
- No change in Tot. Taxa Rich.
- No change in overall CRI-ACD Taxa
- No change in PRM-CRI-ACD of Pred. Taxa
- No change in 2nd-CRI-ACD of all other
Functional Feeding Group Taxa

22. Biotracker for Laurel Falls Run
In the Headwaters, at Site SP7
Total
PRM 2ND Total
Taxa CRI- CRI- CRI- #
Date Richness ACD ACD ACD Indiv.
12/5/2010 7
3 1 2 #15
41 8 15 CRI-ACD
Site SP7 (before dz.)
Qual. Bio.
- low taxa/abundance
Score
12/8/2011 7
3 1 2 #32
36 8 14 CRI-ACD
Site SP7 (after dz.)
QBS
- cont. low taxa/abundance after 1 year
12/5/2011 14 7 4 3 #74
62 18 22 CRI-ACD
(Expect? 8-14 4-7 2-4 3) QBS
Cranberry Run (“Non-impaired” Watershed Ref.)
(Expect? 15-18 8-10 4 4-6 #75-180)
(101+62)/2=
CRI-ACD
81.5
QBS
Optimal Watershed Reference
10/10/2007 18 10 4 6 #180
101 27 35 CRI-ACD
QBS
Isers Run (Ideal/ATI Reference)

23. Isers
Run
(Ideal/
ATI
Ref.)
Cranberry
Run
(Watershed
Reference)
Optimal
Watershed
Reference
(Average
of
Ideal/ATI
Reference
And
Watershed
Reference)
SP7
Each candidate site
will be compared
to:
1. Past to Current
2. “Non-Impaired”
Watershed Reference
3. Optimal
Watershed
Reference
4. Ideal/ATI Reference
SP9 LF10

24. “Tipping Point” for Elklick Creek
mid-stream, at Site SP9
Date
pH Alk Acidity
(su) (mg/l) (mg/l)
6/21/2007 4.60 6.0 32.0
4/8/2009 4.80 6.4 4.4
12/11/2009 5.10 1.8 57.7
3/22/2010 4.80 1.5 10.2
5/20/2010 4.90 2.0 14.5
10/4/2010 6.10 0.0 8.2
Limestone sand dosing started
8/2/2011 6.10 7.3 5.0
5/2/2012 6.60 7.5 6.0
9/11/2012 6.80 10.3 16.4
3/14/2013 6.50 6.0 1.2
Total
PRM 2ND Total
Taxa CRI- CRI- CRI- #
Date Richness ACD ACD ACD Indiv
12/19/2007 3 2 1 1 #3
26 10 5 CRI-ACD
SCORE
12/5/2011 11 5 2 3 #38
65 17 22 CRI-ACD
SCORE

25. Total
PRM 2ND Total
Taxa CRI- CRI- CRI- #
Date Richness ACD ACD ACD Indiv.
12/19/2007 3 2 1 1 #3
26 10 5 CRI-ACD
Site SP9 (before dz.)
Qual. Bio.
- low taxa/abundance
Score
12/8/2011 11 5 2 3 #38
65 17 22 CRI-ACD
Site SP9 (after dz.)
QBS
- large improvement in taxa/abundance
(Expect? 15-18 8-10 4 4-6 #75-180)
(101+62)/2=
CRI-ACD
81.5
QBS
Optimal Watershed Reference
10/10/2007 18 10 4 6 #180
101 27 35 CRI-ACD
QBS
Isers Run (Ideal/ATI Reference)
4/12/12
- No Fish
12/5/2011 14 7 4 3 #74
62 18 22 CRI-ACD
(Expect? 8-14 4-7 2-4 3) QBS
Cranberry Run (“Non-impaired” Watershed Ref.)
Biotracker for Laurel Falls Run
mid-stream, at Site SP9
7/24/13
- > 14 Brookies (3”)
(200-300 placed 5/26)
- 10 Blacknose Dace
Fish Survey Update
4/12/12
- 2 Stocked Brookies (7-8”)
- 8 Blacknose Dace
- 11 Creek Chub
- 3 Mottled Sculpin
- 4 White Suckers
Cranberry Run
SP9, mid-stream

27. Isers
Run
(Ideal/
ATI
Ref.)
Cranberry
Run
(Watershed
Reference)
Optimal
Watershed
Reference
(Average
of
Ideal/ATI
Reference
And
Watershed
Reference)
SP7
Each candidate site
will be compared
to:
1. Past to Current
2. “Non-Impaired”
Watershed Reference
3. Optimal
Watershed
Reference
4. Ideal/ATI Reference
SP9 LF10

28. “Tipping Point” for Laurel Falls Run
at the Mouth, at Site LF10
Date
pH Alk Acidity
(su) (mg/l) (mg/l)
4/8/2009 5.20 7.2 4.8
4/18/2010 5.50 0.4 7.6
Limestone sand dosing started
11/8/2010 6.80 16.6 -2.8
4/29/2011 6.20 11.2 3.0
12/5/2011 6.90 12.0 -4.8
4/12/2012 7.00 12.6 -5.8
12/3/2012 7.00 6.6 -14.8
Total
PRM 2ND Total
Taxa CRI- CRI- CRI- #
Date Richness ACD ACD ACD Indiv
11/8/2010 8 5 2 3 #9
50 9 22 CRI-ACD
12/5/2011 14 9 4 5 #41
98 26 38 CRI-ACD
SCORE

29. Total
PRM 2ND Total
Taxa CRI- CRI- CRI- #
Date Richness ACD ACD ACD Indiv.
11/8/2010 8 5 2 3 #9
50 9 22 CRI-ACD
Site LF10 (before dz.)
Qual. Bio.
- low taxa/abundance
Score
12/5/2011 14 9 4 5 #41
98 26 38 CRI-ACD
Site LF10 (after dz.)
QBS
- large improvement in taxa and abundance
(Expect? 15-18 8-10 4 4-6 #75-180
(101+62)/2=
CRI-ACD
81.5
QBS
Optimal Watershed Reference
10/10/2007 18 10 4 6 #180
101 27 35 CRI-ACD
QBS
Isers Run (Ideal/ATI Reference)
4/12/12
- Stocked Brookie (10”)
- 11 Creek Chub
12/5/2011 14 7 4 3 #74
62 18 22 CRI-ACD
(Expect? 8-14 4-7 2-4 3) QBS
Cranberry Run (“Non-impaired” Watershed Ref.)
Biotracker for Laurel Falls Run
upstream of mouth, at Site LF10
7/24/13
- 23 Blacknose Dace
- 1 Bluegill
- 1 Common Shiner
- 39 Creek Chub
- 13 Mottled Sculpin
- 1 Perch
- 4 White Suckers
Fish Survey Update
4/12/12
- 2 Stocked Brookies (7-8”)
- 8 Blacknose Dace
- 11 Creek Chub
- 3 Mottled Sculpin
- 4 White Suckers
Cranberry Run
LF10 - mouth