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Immunology vi lab_cell_immunol

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Lymphocytes
Methods to Obtain Non-coagulating Blood - Heparin (5-10 IU/ml): activates antithrombin III, blocks the action of thrombin - EDTA: binds Ca2+ - Citrate: binds Ca2+
Marker: Cell Subpopulation CD3 , CD4, CD8 T-lymfocytes CD19, CD20, CD21 B-lymfocytes CD16/CD56 NK cells CD14/DR monocytes HLA-DR; CD25; CD69 activation markers The most important CD antigens of lymphocytes
CD antigens ,[object Object],[object Object],[object Object],[object Object]
Flow cytometer –FACS Fluorescence-Activated Cell Scanner (Sorter)
Flow cytometer
Flow cytometry http://images.google.cz/imgres?imgurl=http://www.abcam.com/ps/CMS/Images/Flow-Cytometry-Diagram2.jpg&
lymfocytes monocytes granulocytes granularity size
CD3 CD4
CD3 CD8
CD3 CD8
Laboratory investigation of lymphocytes ,[object Object],[object Object],[object Object],[object Object]
Delayed-type of hypersenzitivity test
Tuberculin test
 
 
Cell-Mediated Immunity (CMI) tests ,[object Object],[object Object],[object Object],[object Object]
Investigation of function of neutrophils ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
NBT

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Immunology vi lab_cell_immunol