SlideShare ist ein Scribd-Unternehmen logo
1 von 98
Downloaden Sie, um offline zu lesen
1
2
L-Data Science Criticism
Leonid Asipov ‫לעתיד‬ Ph.D
Introduction 4-5
‫מדעית‬ ‫ואמינות‬ ‫מדעיות‬ ‫תוצאות‬ ‫הצגת‬
Botany 5-59
Botany research is not needed anymore
L-Data vs "Expression of cyanobacterial ictB in higher plants enhanced
photosynthesis and growth"
L-Data vs "Enhanced photosynthesis and growth of transgenic plants that express
ictB, a gene involved in HCO 3–accumulation in cyanobacteria"
L-Data vs "A putative HCO transporter in the cyanobacterium
Synechococcus sp. strain PCC 7942 "
The Ci parameter is WRONG
L-Data vs "CO2 Concentrating Mechanisms in Algae: Mechanisms,
Environmental"
L-Data vs: Development of synchronized, autonomous, and self-regulated
oscillations in plant
L-Data vs “The Role of Tobacco Aquaporin1 in Improving Water Use
Efficiency, Hydraulic Conductivity, and Yield Production Under Salt Stress1”
The reason for the lack of photosynthesis after dehydration is lack of water for
enzymes
3
Changes in the properties of reaction center II during the initial stages of photo
inhibition as revealed by thermo luminescence measurements
SYNERGISM OF LIGHT AND FUSICOCCIN ON STOMATAL OPENING"
CO2 not light is the trigger for leaf conductance to gases
WUE CANNOT GROW
There is no main growth control in: transpiration is related to nutrient transport
Cactuses create sugar during the day
There is no CO2: O2 concentration blocks photosynthesis by reverse
CO2 formation
Stomata don't affect photosynthesis
CO2 Channels Have to Enlarge Transpiration
Human industry is nature's best friend
No need for CO2 channel for chloroplast
L-Data vs Rapid assessing of water and nitrogen status in wheat flag leaves
Physics 59-78
Light Speed is not Constant
Relativity Theory Wrong
Matter does not have inner structure
There is no minimum speed needed to fly to space
Gravitational force is related to Surface Area/Mass ratio
4
The particles are not of right form. Sand, salt and any other materials
There is no possibility of creating ideal space
Classical energy equation is wrong : energy is speed/mass
Movement of masses is enough to explain all physical or chemical
phenomena
The nature of forces : Gravitational force
Thermodynamics are false
‫החשמל‬ ‫חליקי‬ ‫ע"י‬ ‫חום‬ ‫ופיזור‬ ‫גדולים‬ ‫מוליכים‬ ‫של‬ ‫אלסטיות‬ : ‫חשמל‬
Light Speed Changes with collisions
ZEREM LO NAHON
RADIO WAVE DIFFERS SPEED NOT FREQUENCY
Light Speed is not uniform, WAVES WRONG
Electricity ERROR: Science fraud
PIGMENT REFLECTION : ABSOBTION OF SPEED
Chemistry 78-82
Periodic Table might be a falsie
Periodic table wrong
Rust
Properties of water molecule : hydrogen is light but concentrated
Hydrogen is small and concentrated : Reactivity
5
water= 6H O1
Biology 82-90
Model of enzyme of a connector can explain DNA replication as a spontaneous
diffusion.
Enzymes does not need any energy for function
Protein construction control by Proteins
No X inactivation exist
GENETICS ARE WRONG
‫אפשריים‬ ‫לא‬ ‫כרומזומליים‬ ‫שחלופים‬
Differentiation of cells : protein - protein replication
DNA IS SINGLE STRANDED
Science 90-95
Science Courthouse
: ‫מדע‬
‫ופעילות‬ ‫מימון‬ ‫צורות‬
Models are proof of understanding : A new science concept
‫קיים‬ ‫לא‬ ‫מימד‬ ‫זמן‬
‫לבד‬ ‫לפרסם‬ ‫צריכים‬ : ‫צודק‬ ‫לא‬ ‫בארץ‬ ‫המדע‬
‫מדע‬ ‫של‬ ‫סטודנטים‬
‫למכור‬ ‫אסור‬ ‫מדע‬
‫מדע‬ ‫של‬ ‫חירטוטים‬
‫במכון‬ ‫אסרים‬ ‫ניסויים‬
6
University Unfair
‫אישורים‬ 96
Introduction
Scientific Honesty and Accuracy
1/5/2011 11:53:12 AM
‫מדע‬ ‫תוצאות‬ ‫הצגת‬
‫מדעית‬ ‫ואמינות‬ ‫יות‬
‫וסטודנטים‬ ‫מדע‬ ‫אנשי‬ ‫בין‬ ‫להטמעה‬ ‫מאד‬ ‫חשוב‬ ‫מושג‬ ‫היא‬ ‫מדעית‬ ‫אמינות‬
‫נתונים‬ ‫עיבוד‬ ,‫מדידה‬ ‫באופן‬ : ‫אמינות‬ ‫לחוסר‬ ‫מקומות‬ ‫הרבה‬ ‫טומנים‬ ‫מדעיים‬ ‫ניסויים‬ ,‫למעשה‬
,
‫אמינות‬ ‫וחוסר‬ ‫לנבוע‬ ‫יכולות‬ ‫מדעיים‬ ‫ממחקרים‬ ‫מוטעות‬ ‫מסקנות‬ .‫המסקנות‬ ‫והסקת‬ ‫הנתונים‬ ‫הצגת‬
‫ומהווים‬
‫אמינות‬ ‫מחוסר‬ .‫המחקר‬ ‫על‬ ‫שהתבססו‬ ‫אחרים‬ ‫וחוקרים‬ ‫עצמו‬ ‫לחוקר‬ ‫משאבים‬ ‫של‬ ‫בזבוז‬
‫מדעי‬ ‫סתום‬ ‫במבוי‬ ‫נמצאים‬ ‫חוקרים‬ ‫הרבה‬ ‫כזאת‬
.
‫אמינות‬ ‫רבדי‬ ‫מספר‬ ‫יש‬ ‫מדעיים‬ ‫ניסויים‬ ‫של‬ ‫לתוצאות‬
:
1
.
‫עצמו‬ ‫הניסוי‬ ‫ביצוע‬ ‫ברמת‬ ‫אמינות‬
‫אפשריים‬ ‫לארטיפקטים‬ ‫לב‬ ‫תשומת‬
‫המדידות‬ ‫ביצוע‬ ‫נכונות‬
2
.
‫ב‬ ‫אמינות‬
‫הניסוי‬ ‫תוצאות‬ ‫עיבוד‬ ‫רמת‬
‫התוצאות‬ ‫בעיבוד‬ ‫טעויות‬
‫המידע‬ ‫כל‬ ‫לעומת‬ ‫הקיים‬ ‫מהמידע‬ ‫חלקים‬ ‫הצגת‬
‫כמותית‬ ‫מול‬ ‫איכותית‬ ‫הצגה‬ ,‫בסטטיסטיקה‬ ‫שימוש‬ ‫חוסר‬
3
.
‫התוצאות‬ ‫הצגת‬ ‫ברמת‬ ‫אמינות‬
‫מידע‬ ‫החסרת‬
4
.
‫שיש‬ ‫הראיות‬ ‫לכל‬ ‫התייחסות‬ : ‫מסקנות‬ ‫הסקת‬ ‫ברמת‬ ‫אמינות‬
‫ש‬ ‫אפשרות‬ ‫שתומן‬ ‫נוסף‬ ‫עניין‬
( ‫במחקר‬ ‫אחריות‬ ‫חלוקת‬ ‫הוא‬ ‫אמינות‬ ‫חוסר‬ ‫ל‬
,‫נתונים‬ ‫עיבוד‬ ,‫מדידה‬
‫מסקנו‬ ‫הסקת‬
)‫ת‬
‫לאיתור‬ ‫קשה‬ ‫הוא‬ ‫שלב‬ ‫בכל‬ ‫אמינות‬ ‫חוסר‬ .‫מספקת‬ ‫מרכזית‬ ‫בקרה‬ ‫והעדר‬
.
‫אמינות‬ ‫וחוסר‬ ‫לטיוח‬ ‫פעמים‬ ‫הרבה‬ ‫מובילה‬ ‫במחקר‬ ‫המשתתפים‬ ‫האנשים‬ ‫כל‬ ‫בין‬ ‫אובייקטיביות‬ ‫העדר‬
‫של‬
‫בתוצאות‬ ‫שמעוניינים‬ ‫השונים‬ ‫הגורמים‬
‫אחרות‬ ‫ולא‬ ‫מסויימות‬
.
7
Botany
Botany research is not needed anymore
8/21/2011 12:38:48 PM
‫הידע‬ ‫כל‬ ‫את‬ ‫כבר‬ ‫יש‬ : ‫בוטניקה‬ ‫יותר‬ ‫צריך‬ ‫לא‬
.
‫יותר‬ ‫נחוצים‬ ‫שלא‬ ‫נושאים‬ ‫צורך‬ ‫ללא‬ ,‫סתם‬ ‫חוקרים‬ ‫המדענים‬
.
‫המדענים‬ .‫נוסף‬ ‫למחקר‬ ‫צורך‬ ‫אין‬ ,‫מספיק‬ ‫ידוע‬ ,‫הצמח‬ ‫מדעי‬ ‫של‬ ‫בנושאים‬
‫הביתה‬ ‫לחזור‬ ‫יכולם‬
‫התעסוקה‬ ‫מלשכת‬ ‫קצבה‬ ‫ולקבל‬
.
‫שאנושות‬ ‫שנחוץ‬ ‫מה‬ ‫כל‬
-
,‫גודל‬ : ‫רצויות‬ ‫תכונות‬ ‫עם‬ ‫זנים‬ ‫מטפח‬ ‫הזעים‬ ‫בנק‬ .‫צמחים‬ ‫של‬ ‫זרעים‬ ‫בנק‬
‫מלח‬ ‫ריכוז‬ ,‫סוכר‬ ‫ריכוז‬ ,‫פירות‬ ‫של‬ ‫מקסימלי‬ ‫מספר‬
,
‫הטיפוח‬ .‫גדילה‬ ‫מהירות‬ ,‫חלבון‬ ‫ריכוז‬
-
.‫פשוט‬
‫הצמחי‬ ‫את‬ .‫צמחים‬ ‫מגדלים‬ ‫קדם‬ ‫בימי‬ ‫כמו‬
‫הטיפוח‬ .‫המשך‬ ‫בדור‬ ‫מרבים‬ ‫טובים‬ ‫הכי‬ ‫ם‬
-
‫דורות‬ ‫מספר‬
,
‫האחרון‬ ‫הדור‬
-
‫פעם‬ ‫זרעיים‬ ‫קונים‬ ‫חקלאים‬ .‫זנים‬ ‫והרבה‬ ‫מינים‬ ‫הרבה‬ ‫יש‬ ‫הזרעיים‬ ‫בבנק‬ .‫לזרעיים‬
‫אחת‬
-
‫לזרעיים‬ ‫שגודל‬ ‫צמחים‬ ‫של‬ ‫מסויים‬ ‫אחוז‬ ‫יבול‬ ‫בכל‬ ‫לחקלאי‬ ‫יש‬ ‫כך‬ ‫אחר‬
.
‫ד‬ ‫נג‬ .‫וטבק‬ ‫שום‬ : ‫טבעיים‬ ‫אמצעים‬ ‫יש‬ ‫מזיקים‬ ‫חרקים‬ ‫נגד‬
‫עבודת‬ ‫של‬ ‫אמצעים‬ ‫יש‬ ‫שוטים‬ ‫עשבים‬
‫השוטים‬ ‫והצמחים‬ ‫בשדה‬ ‫בתחרות‬ ‫מנצחים‬ ‫שמגדלים‬ ‫הצמחים‬ ‫רוב‬ .‫אדם‬
‫מופיעים‬ ‫לא‬
.
‫שנים‬ ‫במספר‬ ‫פעם‬ ‫בשדה‬ ‫אחרות‬ ‫וקטניות‬ ‫שועית‬ ,‫אפונה‬ ‫גידול‬ ‫עם‬ ‫אפשריים‬ ‫טבעיים‬ ‫דשנים‬
.
‫השקייה‬
-
‫השוטים‬ ‫העשבים‬ ‫כמות‬ ‫את‬ ‫שמקטינה‬ ‫טפטוף‬ ‫עם‬ ‫רגילה‬
-
‫ה‬ ‫לייד‬ ‫ההשקייה‬ ‫כי‬
‫צמחים‬
‫לעשבים‬ ‫אור‬ ‫שממסכים‬
.
‫אקמית‬ ‫השכלה‬ ‫דורשים‬ ‫לא‬ ‫ייחורים‬ ‫גידול‬ ‫כגון‬ ‫פשוטים‬ ‫בוטניים‬ ‫אמצעים‬
.
‫הצמחים‬ ‫תורת‬ ? ‫כסף‬ ‫קובלתם‬ ‫מה‬ ‫על‬ ?‫תרומתכם‬ ‫מה‬ ‫בוטנאים‬
-
.‫כלום‬ ‫הוסיפה‬ ‫לא‬ ‫המודרנה‬ .‫עתיקה‬
L-Data vs "Expression of cyanobacterial ictB in higher plants enhanced
photosynthesis and growth"
Leonid Asipov
11/15/2012 10:57:26 AM
From letter to the editorial director of the magazine…
"My Name is Leonid Asipov. I am the co-author of the article and the worker
which accomplished the experiments. The data results are insignificant and no
conclusions are possible on basis of the results. Whole experiments were
neglected by the main author of the article and the conclusions are scientific
lies… "
8
TOPICS
1. ONLY PARTIAL DATA WAS PRESENTED. THE ADDITIONAL PLANTS
SHOW NO SIGNIFICANCE RELATIVE TO WT.
2. SECOND GENERATION OF PLANTS, GROWN OUT OF THE BEST 1-ST
GENERATION PLANTS, SHOW NO SIGNIFICANCE AT ALL.
3. FALSE CONCLUSION IN ABSTRACT OF THE ARTICLE, ACCORING
TO THE AUTHOR,
THE ENHANCEMENT OF PHOTOSYNTHESIS IN ONLY AT LIMITING
CO2 CONDITIONS,
HOWEVER, THE RESULTS (BASED ON ONLY 4 PLANTS) SHOW THAT
AT ALL CO2 LEVELS, THE TRANSGENIC PLANTS ARE BETTER. NOTE
THAT MOST OF THE DATA (THE REST 33 TRANSGENIC PLANTS) WAS
NOT PUBLISHED.
4. No significance tests were calculated. The group of transgenic plants is too
small(4 plants).
THE REAL DATA: EMPIRIC MEASUREMENTS OF GAS-EXCHANGE OF
LEAVES: WT VS TRANSGENIC PLANTS.
Time of day during measurement
The measurement is Gas-Exchange of single leaves. Transgenic and wt plants are
similarly measured by the gas-exchange device, in elevating CO2 concentrations
and in similar temperature and light conditions. The time of day of the
measurement of each plant was different. Part of the plants were measured during
morning and part, during the evening.
The difference of the Photosynthesis rate between the different times of day is
great. During the evening, the plants were photosynthesizing may hours already
and part of the enzymes needs regeneration. Moring is hours of highest
photosynthesis rates. Note that there is large differences between the
photosynthesis and transpiration rates of the plants. Part of the large differences
is due to different time of day during measurement.
9
Scientific conclusions
The plants are to be measured in equal conditions: equal time of day and equal
age. When the experiment is large, the task is impossible. Since the gas-exchange
measurement device is based on a single leaf and is expensive, in addition to long
measurement time of the CO2 curve (at least 15 minutes for each plants +
acclimation time) and large number of plants demanding other method for plant
screening for better photosynthesis and transpiration properties. Measurement in
a single CO2 concentration is partial solution for possible measurement of many
plants in short period of time.
The results of the article: partial data was presented.
The transgenic plants are to be considered similar "treatment" and a large number
of plants are to be measured and T significance tests are to be calculated. If some
particular transgenic plant is for any reason considered to be "other treatment",
the plant is to be cloned to next generation and large amount of plants are to be
measured in order to perform T significance tests.
Second generation of arabidopsis(NOT PUBLISHED DATA)
Second generation was cloned of the 4 considered transgenic plants and the
results showed no significant difference relative to wt plants.
THE PAPER WAS PUBLISHED WITH WRONG CONCLUSIONS BASED
ON PARTIAL DATA, WHEN ALL DATA WAS ABUNDANT. THE
SCIENTISTS ARE LIERS.
Presentation of all the data
The real Gas-Exchange data. The first experiment included 37 transgenic
arabidopsis plants vs 7 wt plants. The published chart however consists of only 4
transgenic plants. The researchers have neglected all the rest 33 plants and
CONCLUDED FALSE conclusions, according to which the transgenic protein
has positive effect on photosynthesis of plants.
The absence of statistics
The variation of the plants is high and T significance tests of all the 37 plants vs
11
wt show no significance at all. The experiment of protein transformation is to be
considered similar “treatment”. The 4 top plants were cloned to the next
generation and similar tests were conducted. No significance was seen.
Original chart published in paper: only 4 plants of 37 transgenic plants were
published.
Note the inconsistent conclusion in abstract of the article : “photosynthesis was
enhanced under limiting and not under saturating CO2 concentrations…” ALL 4
transgenic plants are better photosynthetically at ALL CO2 points(NOTE THE
REST 33 PLANTS WHICH ARE NOT).
All 37 transgenic plants. Only top 4 were published. The rest of the plants are
with large variance. Better or worse than wt. Wt is also variant. Note that only 6
wt plants were measured. More plants, more variance.
11
THE PUBLISHER DID PUBLISH ONLY 4 TRANSGENIC PLANTS.
REST OF THE DATA WAS HIDDEN. THE SCEITIFIC FRAUD WAS
CAUGHT!!!!!!!!!!!!!!
2nd generation arabidopsis (number of plants > 30). UNSIGNIFICANT
DIFFERENCES BETWEEN WT AND THE TRANSGENIC.
The protein was also tested in other plants. Similar gas-exchange experiments
were conducted.
12
The protein does NOT enhance photosynthesis or prevent transpiration…
Soybean experiment.
The theory behind the article is wrong.
Theoretic criticism.
The CO2 channel in chloroplast is not needed : the membrane keeps the unique
environment in chloroplast.
Channels conduct to and from the chloroplast. The reason of the membrane is to
block particles from diffusion from the chloroplast to the cytoplasm.
Additional CO2 channel in chloroplast membrane would have forced diffusion of
small and medium particles from the chloroplast to the cytoplasm, slowing the
enzymatic processes in the chloroplast.
The diffusion of CO2 to chloroplast is fast due to large surface area/volume ratio.
The main slowing process is diffusion through the cytoplasm.
The surface area of a chloroplast is large relative to volume…. The main
obstruction to diffusion of CO2 from the air the chloroplast is cytoplasm volume
and cytoplasm membrane… The distance of diffusion is the whole cytoplasm…
The distance of diffusion through the chloroplast is small and don't forget the
large surface area / volume ratio of the chloroplast which cause fast diffusion
through the membrane. The conclusion is that no CO2 channels are needed to
13
chloroplast membrane. Addition of CO2 channel, don't enhance any processes in
the plant cell…And might slow some processes by diffusion of needed particles
from chloroplast to the cell cytoplasm.
Bacterial proteins does not express properly in higher organisms. Note folding
proteins which are needed.
Expression of bacterial protein in higher plants: wrong concept. It is well known
in science that proteins from eukaryotes cannot be easily expressed in
prokaryotes. In order to create the antibodies, mammal or fly biological systems
are needed. The 3rd structure of the proteins need folding proteins. Spontaneous
expression of a bacterial protein in plants is not likely to produce a proper
protein. The folding proteins of the plant are not compatible. A whole set of
additional proteins are needed in order to create a proper protein. The ribosomes
of bacteria are different, suggesting the building of the proteins is as well.
Possible even the genetic code differs and codons code to other amino acids.
The task of the researchers is to prove the protein function, 3-d structure and
proper attachment of the protein in the chloroplast membrane.
Since the protein was described as "putative", and no 3d structure was proved, as
well as no positioning in the chloroplast membrane, it is impossible to claim any
biological effect. The foreign bacterial protein may be malfunctional and not
even expressed in chloroplast.
The difference of the plants in sampling time
The experiment was sampled during 2 months and life of arabidopsis plant is
about 3 months only. The first plants were the best transgenics, the control plants
were measured during the last 4 days of experiment. The control plants were 2
months older than the best (published) transgenics. The difference of the plants is
mostly at transpiration parameter, not photosynthesis, and the root system of the
transgenic plants which are older by two months is obviously larger, and thus the
transpiration. The difference between the transgenic and wt plants is due to age,
no genetic differences.
External CO2 vs Photosynthesis (wt vs best transgenics)
14
External CO2 vs transpiration: transpiration of wt plants is larger (most possibly
because of root system)
The ages and sampling dates :
15
PROPER SCIENTIFIC WAY
The proper way of molecular biology is protein crystallization to prove proper
function : CO2 channel. CO2 channels, as any channel is a pipe-like protein. The
pipe is only molecule size specific. CO2 channels conduct medium and small
size molecules such as water, CO2, H and other.
Proof of protein expression and positioning in chloroplast membranes. The
protein has to be proven to have proper channel function and positioning.
ONLY AFTER PROPER MOLECULAR PROOF, transgenic plants are to be
grown and compared with wt plants. BEFOREHAND we cannot even ask any
scientific question, since the protein may be not a channel, not properly built by
the plant protein building machinery or not to be positioned in the chloroplast
membrane. GUESSING is not proper science.
THEORY HAS TO PERCEDE the scientific research with all the possibilities of
the results.
16
The Ci parameter : wrong science
The Ci parameter is wrong. Photosynthesis is linked to X and Y axis on Ci-Photo
charts.
Proper comparison is possible between curves is possible at similar X points. The
empirically changed parameter is the external CO2 concentration (CO2R).
Photosynthesis and Transpiration are biological parameters affected by the
external CO2 change. The proper way to compare the effect of external CO2 on
photosynthesis and transpiration is to present the external [CO2] on the X axis
and plot 2 separate curves for transpiration and photosynthesis.
The Ci parameter is wrong :
Ci =
External [CO2] * STOMATA APERTURE AS TRANSPIRATION * pressure /
PHOTOSYNTHESIS * leaf temperature
One Ci, different Photosynthesis and transpiration values. If transpiration and
photosynthesis is high, the ci is same if transpiration and photosynthesis is low.
When plotting relative to photosynthesis, points are compared at different X
values, which are related to Y (PHOTOSYNTHESIS) .
The ci parameter cannot tell anything about the real properties.
Since it is basically ratio of TRANSPIRATION/PHOTOSYNTHESIS.
PHOTOSYNTHEIS and TRANSPIRATION IS TO BE PRESENTED AS
FUNCTION OF EXTERNAL CO2 CONCENTRATION.
L-Data vs "Enhanced photosynthesis and growth of transgenic plants that
express ictB, a gene involved in HCO 3–accumulation in cyanobacteria"
17
11/15/2012 11:46:40 AM
Criticism
Under low relative humidity, the stomata are more open, and there is more
transpiration. What is the limiting photosynthesis factor under low humidity
relative to high humidity? The ONLY factor which is to consider is transpiration.
Under lower humidity concentrations, there is higher concentration of CO2 in the
air, and thus photosynthesis is ENHANCED.
The protein works more at lower air humidity, ONLY AT HIGHER CO2
concentrations.
The protein should be a difference at limiting CO2 conditions, not low humidity.
Internal article inconsistency.
Since there almost no PHOTOSYNTHESIS dependence on stomata aperture(Fig
1),
The photosynthesis under any humidity is relatively constant. The only factor is
humidity which lowers the CO2 concentration of the air. According to the article,
at lower CO2 concentrations, WT plants are better. WT is the better CO2
conductor than the transgenic plants.
The "faster" growth of the transgenic plants at lower humidity, most possibly is
accompanied with higher transpiration rates. Why the Water USE Efficiency
parameter was not calculated?
The transpiration was reported to be similar between the wt and the transgenic
plants. Why more growth at lower humidity, at higher CO2?
"CO2 compensation point": the plant is breathing more CO2.
More energetically demanding plant.
18
At lower CO2 concentration, there is almost no photosynthesis. The plant is
breathing and CO2 is diffusing from the plant to the air. If the plant is more sugar
demanding there is more breathing and thus, there will be more CO2 absorption
when the external CO2 is lower. The expression of additional protein in
energetically demanding. The plants breathe more, thus lower compensation
point, due to higher CO2 in the leaf.
The CO2 compensation point is related to the two first measurements of
Photosynthesis and Transpiration. The usual CO2 concentration is 50 and 100
ppm.
The compensation point is a calculated point between 50 and 100 ppm at which
photosynthesis becomes more than 0. If plant breathes more, at similar
photosynthesis,
There is more CO2 after the air travels through the chamber, and the result is
LESS photosynthesis. Due to breathing, there could be two reasons for lower
compensation point.
1. More photosynthesis
2. Bore breathing, which leads to CO2 diffusion from the leaf to the air out of the
plant.
The advice is to measure photosynthesis at higher concentrations, when to the
additional from plant breathing CO2 there is less meaning.
The real DATA
The number of plants is only 4 transgenic plants vs 1 wt. How is it possible to
conclude anything of so few plants? Part of the transgenic plants are not better,
how to explain? If part of transgenic plants are to consider other properties than
the rest of the plants, the plants should have been cloned to the next generation
and large number of plants were supposed to be sampled. Conclusions are to be
made on basis of T significance tests of many plants and not on only 4 transgenic
and 1 wt plants. The Arabidopsis conclusion was based on 2 transgenic and 1 wt
plant. Conclusions could have been made due to too small plants group. The wt
plants are relatively at the average of the transgenic plants CO2 curve. Why the
conclusion was that the transgenic plants are better, when some transgenic plants
was not?
19
Why DO we care about photosynthesis at limiting conditions. The real conditions
at fields are not limiting. There is no advantage of transgenic plants and NO
transpiration difference.
The stomata respond to CO2 with closure. Better photosynthesis, lower CO2,
more stomata aperture and MORE TRANSPIRATION. If the plants are better at
Lower humidity, MORE TRANSPIRATION is EXPECTED but not reported.
CO2 is the main trigger of stomata aperture, and photosynthesis is related to CO2
concentration in the leaf and near the stomata. At high photosynthesis, more
transpiration is expected.
DATA INSTABILITY:
There was too many samples and the AIR CO2 concentration at every sample is
not clear. Either the researchers were only slightly changing the AIR CO2
concentration, or there was no enough time of acclimation to the external CO2
conditions. The differences in Ci is at almost every sample, was there a real
change is the air CO2? MORE ACCLIMATION TIME IS TO BE GIVEN TO
THE PLANT AFTER CO2 changes in the air. The Ci parameter has to be
constant and only then the sample to be taken. There are more than 20 samples of
every plant and the concentration of CO2 was changed between 50 and ~800
(UNMENTIONED AT ARTICLE) . Was the external CO2 changed at every
point? Why there is more than one sampling at a single CO2 point?
Acclimation times and CO2 concentration points were unmentioned at Materials
and Methods.
Growth results
There was too few plants to conclude any conclusion. Conclusions are to be
based on large number of plants and statistical T tests are to be performed.
The growth in lower humidity (which is seen as higher photosynthesis measured
at the external to leaf air) would have affected transpiration. Lower CO2 of the
air is causing stomata aperture to grow, and thus the transpiration rate is
HIGHER.
The transgenic plants are more water DEMANDING, which is not positive to
21
agriculture.
"The experiments were held 6 times", where are the results? Why the statistical
data was not presented?
70% RH is wide and common humidity. Under many agricultural applications
there is no advantage of the protein. WHY TO ADVANCE PARTIAL AND
UNCLEAR SOLUTION which is not better at most cases?
Conclusions
1. The better photosynthesis at lower humidity is not logical. There is more CO2
in the air. Better CO2 assimilation is of WT plants. The protein enhances CO2
transport only at higher CO2 concentrations. INCONSISTENCY. The transgenic
plants do not grow better at lower co2 concentrations, but at higher ones.
2. The limiting CO2 concentrations are not real at earth. Normal air has no
limitation of CO2 (~400ppm). No advantage of the protein.
3. NO EFFECT ON TRANSPIRATION WAS REPORTED, which is
impossible. More photosynthesis, more stomata aperture, since stomata are
triggered by CO2 concentration.
4. Higher transpiration rates are expected from transgenic plants due to higher
photosynthesis. The transgenic plants are WATER DEMANDING.
1. The water use efficiency was NOT CALCULATED.
2. Experiments were based on SMALL NUMBER OF PLANTS.
3. NO STATISTICS WERE PRESENTED.
CONCLUSIONS ARE IMPOSSIBLE BASED ON ONLY 4 TRANSGENIC
AND 1 WT PLANT.
TRANSGENIC PLANTS AS PRESENTED ARE MORE WATER
DEMANDING AND GROW BETTER AT HIGHER CO2
CONCENTRATIONS (LOW HUMIDITY)
The CO2 is the main stomata trigger. More photosynthesis, more transpiration.
21
Fig 1
Decoupling of photosynthesis and transpiration, after alterations of CO2
concentration, proves that there is no significant dependence of stomata and
photosynthesis. The CO2 diffusion is fast and stomata limit mostly evaporation,
while the photosynthesis is almost not affected by stomata change of
conductance.
L-Data vs "A putative HCO transporter in the cyanobacterium
Synechococcus sp. strain PCC 7942 "
11/15/2012 11:54:34 AM
CO2 CHANNELS ARE NOT NEEDED IN BACTERIA
Bacteria have large surface area/volume ration, and thus there is no channels
needed to conduct water or CO2. The mutant, which need more CO2 for proper
photosynthesis rate, can be defected in any photosynthetic-related protein, not
especially CO2 channels.
If there are CO2 channels in bacteria, why to add a channel to a chloroplast?
22
The channels in bacteria would have forced small molecules such as H, H2O,
CO2
And many more molecules to diffuse out of the bacterium. To cytoplasm wants
to
Remain UNIQUE and thus any channel force larger particle concentrations.
EUKARYOTIC cells are large, with SMALLER surface area/ volume ratio, and
thus
Channels are necessary.
A CHLOROPLAST IS A CYANOBACTERIA. CHANNELS ARE
CONSISTENT.
THE TERM "CCM"
"CCM" CO2 concentrating mechanisms. It is impossible to concentrate CO2,
ONLY to transport faster to the cell. The diffusion rate is related to
Concentration of CO2 and to the speed of conduction of CO2 by the cell wall.
There is no artificial concentrating mechanisms, only diffusion.
THE PROTEIN MIGHT BE NOT A CHANNEL
Since the "putative" protein is not necessarily a CO2 channel, which are not
expected to be expressed in bacteria, expression in plant will not initiate any
change.
First the structure of the protein to BE crytallized and proved AS a CHANNEL,
pipeous proteins are channels.
THEN, the protein has to be proved to be positioned in the chloroplast
membrane.
ERROR IN FIGURE : "External Ci" concentration. External = CO2R. There is
NO Ci in
cyanobacteria. Ci is concentration of CO2 in the leaf.
23
The transporter does not influence the concentration, only rate of acclimation to
the CO2 change surrounding the cell. The external concentration of CO2 is large
(mmol) and the photosynthesis is slow (micromoles) and therefore, after change
in external CO2,
ACCLIMATION TO ALTERATIONS IN CO2
There is acclimation during which the inner cell concentration becomes as the
external
concentration of CO2. The uptake is slow, and during uptake, the cell
concentration
barely changes. The rate of diffusion of co2 is LARGE, therefore, the cell
concentration is
CONSTANT and similar to OUTER (IN CASE OF EQUAL OTHER SOLUTE
CONCENTRATION).
If the acclimation time after change in external CO2 is long, there should be no
difference between the mutants and the wt. Since in the mutant there is already
full steady state photosynthesis (starting at 15mM HCO3 or 6 mM CO2), the rate
of
diffusion is BEYOND the scope of the limitation of photosynthesis. The real
limiting
factor of the mutant relative to wt is SOME PROTEIN NEEDED FOR
PHOTOSYNTHETIC
REACTIONS. The diffusion of CO2 is already at saturation, and all the
cytoplasm is
with concentration of CO2 similar to the external. Since, the mutants need more
CO2 and not only more acclimation, the mutants are impaired in photosynthesis
Proteins expression. LESS PROTEINS, LESS PHOTOSYNTHESIS.
HIGHER CO2 FOR SILIMAR PHOTOSYNTHESIS.
TIME BETWEEN THE MEASUREMENTS(CHART 2)
WHAT WAS THE TIME BETWEEN MEASUREMENTS(AFTER
ALTERATIONS IN EXTERNAL CO2)?
In CASE of the protein as a CO2 channel, more acclimation, would result in
24
similar
PHOTOSYNTHESIS at similar [CO2]. At shorter times, there is still uneven
[CO2] between
The surroundings and the cytoplasm. More CO2 needed for faster diffusion and
similar
Photosynthesis. In case, time does not force any similarity of
PHOTOSYNTHESIS of wt and
the treatment, THE PROTEIN IS NOT A CHANNEL .
"The protein encoded by ORF467 contains 10 putative
transmembrane regions and is inner-membrane-located."
WHERE IS THE PROOF?
A PUTATIVE REGIONS WHICH ARE SIMILAR TO OTHER STUDIED
PROTEINS ARE NOT TO BE CONSIDERED AS PROOF FOR PROTEIN
FUNCTION. THE DATABASE ANALYSIS OF PROTEINS ARE MERELY A
CLUE TO THE REAL PROTEIN FUNCTION AND NO CONCLUSIONS
CAN BE CONCLUDED FROM ANY FORMAL SIMILARITY OF THE
GENETIC CODE AND ANY KNOWN PROTEINS. THE MOTIFS ARE NOT
PROOF BUT SPECULATIONS.
REAL PROOF = CRYSTALLIZATION AND POSITIONING OF GFP
FOLLOWINF MICROSCOPY.
Channels/ carriers
NO ACTIVE CO2 CHANNELS ARE EXPECTED. THE OUTER CO2 is
mmoles, and the photosynthesis is micromoles. THEREFORE, the DIFFUSION
IS NOT ACTIVE.
The "channels" are pipes which enhance membrane transport. The pipes are
unspecific.
The size of molecule is the only limiting factor. Large Surface area/ volume of
bacteria,
lead to the unnecessary existence of any channels in bacteria.
CONCLUSIONS
25
1. The protein might be not a channel.
2. If there are channels in bacteria, there are also channels is chloroplast.
3. NO PROOF FOR FUNCTION
4. NO "CONCENTRATING MECHANISMS" EXIST
5. ACCLIMATION BETWEEN MEASUREMENTS UNMENTIONED.
6. NO PROOF FOR POSITIONING
The Ci parameter is WRONG
11/15/2012 12:21:54 PM
Mathematical function is set of points to compare at Y axis at similar X.
The Ci parameter includes the photosynthesis.
WHY TO INCLUDE TO X THE Y axis of the CI CURVE?
The Ci curve is comparison of PHOTOSYNTHESIS VALUES AT
DIFFEARENT Ci,
When Ci includes already photosynthesis, resulting change in X values to
different values.
The X of the compared points is not same. COMPARISON IS WRONG.
The calculation of Ci is artificial (not measured) calculation of a parameter
similar to the measured parameter CO2R (External CO2). The true parameter
which should be set to X axis is CO2R. COwR is the manually or automatically
changed parameter and as function of CO2R changes in photosynthesis and
transpiration do happen in the plant leaf.
The artificial parameter Ci, calculates some a value close to CO2R, and based on
Transpiration/ photosynthesis ratio, leaf temperature, and pressure. The artificial
CO2 concentration is similar to CO2R, but less usually. The ERROR of Ci is that
the CO2 concentration of the leaf is not related to STOMATA APERTURE. The
diffusion of CO2 is barely limited by stomata. The only limitation of stomata is
on transpiration. Therefore, the Ci is artificial and wrong. See "DECOUPLING
OF PHOTOSYNTHESIS AND TRANSPIRATION AFTER ALTERATIONS
26
IN EXTERNAL CO2".
ORANGE: IRRADIATION
PURPLE: CO2 CONCENTRATION OF AIR
GREEN: PHOTOSYNTHESIS
BLUE: TRANSPIRATION
Figure 1: DECOUPLING OF PHOTOSYNTHESIS AND TRANSPIRATION
AFTER CHANGES IN CO2(Chart from Leonid Asipov MSc THESIS).
The transpiration changes during a long time of 30 minutes or more after the
change in CO2.
Photosynthesis changes almost instantly(30 seconds), During the change in the
transpiration rate, the photosynthesis does not change. During the period of
change of the transpiration, Stomata change the aperture, process which does not
affect the photosynthesis rate. The conclusion is that stomata mainly affect the
transpiration rate due to differences in concentration between the leaf the air and
slower speed of water diffusion (MORE LIQUID THAN CO2). CO2 diffusion is
fast and almost not limited by stomata.
27
The BEST parameter is the real parameter CO2R, presented with Transpiration
or Photosynthesis on separate curves.
Ci unclear results : no conclusions
EXTERNAL CO2 VS PHOTO
28
EXTERNAL CO2 VS TRANSPIRATION
CONCLUSION : TRANSIRATION IS THE CAUSE FOR CHANGE OF CI ,
NOT PHOTO.
.
29
L-Data vs "CO2 Concentrating Mechanisms in Algae: Mechanisms,
Environmental"
11/15/2012 12:01:42 PM
CCM mechanisms are not likely. CO2 is high concentration in the air, relative to
plant. Artificially to elevate concentration is an impossible mission with an
inefficient nature.
Why to artificially elevate the concentration of CO2 in the chloroplast? The
chloroplast's nature sugar consumption produces more CO2, thus elevating the
CO2 concentration near the chloroplast. The discussed CO2 may be the reason
scientists thought that the chloroplast possesses CO2 concentrating mechanisms.
The nature works with diffusion. It is impossible to artificially change
concentrations opposite to the substance concentrations. Water, salt, CO2, sugar,
all substances diffuse naturally in the plant and the plant adapts to certain
concentration of the substances.
The citrated work does not prove existence of CCM, but states the CCM may
exist. The science does not like science fiction.
CO2 channeling. Channels are proteins which conduct particles according to the
concentration gradient. The PIPES, are normal protein pipes.
There are no transporters but PASSIVE, with GRADIENT.
ENERGY IS MOVEMENT. MATTER DISSOLVING TO SMALLER
PARTICLES ENLARGES HEAT CONDUCTANCE. CHAOS. THE MATTER
IS STRUCTURE. THE CELLS CANNOT ENLARGE THE REACTIVITY( BY
DISSIPATION OF SUGARS) AND EXPECT TRANSPORT OF
SUBSTANCES AGAINST THE CONCENTRATION GRADIENT. THE
DIFFUSION OF ONE SUBSTANCE IS BARELY RELATED TO THE
OTHER. THE DIFFUSION IS RANDOM. THE DEPENDENCE IS ONLY AT
HIGH CONCENTRATIONS, WHEN THE VOLUME IS A LIMITING
FACTOR. THUS, NO ACTIVE TRANSPORTERS EXIST. REGULAR
DIFFUSION ONLY. THE PLANT IS BUILDING THE ORAGNIZM WITH
PARTICLES SUCH AS SUGAR, PROTEIN OR FAT, NO "ENERGY" EXIST
31
IN MATTER. SOME REACTIVITY (HIGH HEAT CONDUCTANCE DUE TO
SMALL PARTICLES) IS NEEDED FOR DISSIPATION OF SUBSTRATE
FROM ENZYME OR TEMPORARY LIFE OF BIO SUBSTANCES,
WHICH IS VITAL FOR NEW CELL REBUILDING.
L-Data vs: Development of synchronized, autonomous, and self-regulated
oscillations in plant
11/14/2012 6:27:04 PM
P.S
I was a student of the publishers and the data abundant. Please consider the
papers I enclose scientifically.
SUMMARY
NO SHORT TERM OSCILLATIONS OF PLANT TRASPIRATION
EXISTENT. THE MEASUREMENT EFFECT IS DUE TO MEASUREMENT
INACCURACY
Flaws in the reported article.
1. SIMILAR OSCILLATIONS WITHOUT IMPORTANCE DAY/DARK
2. NO SHORT TERM OSCILLATIONS AT GAS EXCHANGE
MEASUREMENT
3. OSCILLATIONS ARE SMALLER THAN THE NORMAL INACUURACY
OF A ELECTRONIC SCALE: +- 2 grams (ACCORDING TO PRODUCER)
Data from Tedea-Vishay 1040 C3 transducer. Total error : 0.02% of rated output.
0.02% of 5000 grams (average plant) is +- 1 gram.
The total error of course is measured in ideal conditions of humidity, noise,
temperature and wind. Normal experimental conditions of course
Elevates the noise. THE NOISE OF WEIGHT SCALE IS AT LEAST +-1 gram.
31
Actual noise: +- 2 grams.
1. Inability to differentiate between oscillations due to fluctuation in light,
temperature, humidity and water availability and the reported "spontaneous"
oscillations. The reported experiments were held in a greenhouse with fluctuating
irradiation parameter. The claim for "spontaneous" transpiration oscillations is
impossible due to unstable environment. The control is a wet blanket positioned
on a similar scale weight. The short term fluctuations of the control is without
any significant correlation to plants, suggesting the short term fluctuations are
not related to temperature or irradiation, but another sensor of transpiration (gas
exchange of a single leaf) suggests the total absence of oscillations on basis of
short term periods of less than 5 minutes. Since the temperature or light was not
measured by any other sensor than the scale, an artifact can be speculated. The
control should have been additional sensor such as light, temperature or humidity
simultaneous to the weight measurement. The absence of a proper control to the
experiment suggests improper scientific approach basing on a single device. The
electronic scale has certain accuracy. The minimal accuracy stands for 0.3 %
from the weight, and about +- 2 grams. The size of the reported oscillations is
about +- 1 gram. Less than minimal accuracy, suggesting the short term
oscillations are noise.
2. The weight measurement should have been held in controlled environment, to
avoid any fluctuations of li8ght as effect on transpiration. Since the experiment
was held in unstable light, the oscillations may be due to external irradiation
fluctuations…
The experimental setup
Fig 1A: Whole plant on a weight scale for continuous transpiration measurement.
Readings every 10 seconds, average to 3 minutes.
32
Fig 1B: Gas-exchange measurement of a single leaf. Points are measured
every 2 minutes.
The size of the oscillations is similar to normal measurement noise
33
Fig2: Weight scale measurement of a whole plant. Inaccuracies unseen since
the total weight of the plant is much larger than the noise.
Fig 3: Small noises are seen after differentiation since the transpiration value
34
between each point and the following point is only ~10 grams. The noise is
0.3% of total plant weight (about +-2 grams). Noise is 20% of measured value.
Significance of results exists when value of transpiration is much larger than
noise.
On scale of 30 minutes the extent of the noise is 2.5%. After averaging on the
range of 30 minutes, the oscillations are correlated with the irradiation levels (Fig
6).
PAPER 1: DATA FROM MSC THESIS ACCEPTED BY THE CAMPUS OF
REHOVOT.
Simultaneous measurement of Gas-Exchange and Scales. Additional sensor.
The scale is only one sensor. Artifact regarding the scale itself is to consider.
The control of the scale is transpiration measured by gas-exchange method.
The oscillations are expected to be larger, since a single leaf is less synchronized
than the whole plant. The more stomata is measured, the less oscillations are
expected, since the oscillations of single stomata is averaged by the experiment.
The whole plant transpiration can be considered as NON oscillating
PARAMETER.
Experiment Setup
1. Load Cell A whole tomato plant is positioned on a load cell in a water
container. The rate of transpiration is calculated from weight loss of the plant and
the container.
2.Gas-exchange The rate of transpiration of the same plant is simultaneously
measured using gas exchange method on a single leaf in the upper part of the
plant. The experiment was made on a well watered plant during a whole day in
natural light.
Results
35
Fig 4: Simultaneous measurement of transpiration using load cell and gas
exchange methods.
Conclusions
The oscillations are the similar size during the night and the day periods.
The forecast is that during high transpiration period, the oscillations are supposed
to be higher. However the oscillations, are not different. The fact that the
oscillations are abundant during the night suggests that the oscillations are due to
measurement artifact, which is basically inaccuracy of the weight scale.
The accuracy is 0.3 % which is about +- 2 grams.
The reported short term oscillations are about the same size, ~+-1 gram.
The lack of any oscillations during the night period observed in gas-exchange
measurement, suggests that the oscillations are an artifact related to the electronic
scale.
During the day period, there are long-term light-correlated oscillations seen also
in gas-exchange experiment.
36
The fact that the experiment was held in natural light, adds uncertainty to the
experiment. There is a degree of oscillations related to light fluctuations. The
scale weight oscillations are hardly differentiated from light oscillations,
however, the nocturnal oscillation of the weight suggests that the oscillations are
an artifact.
The better experiment is to measure in controlled environment, with constant
light levels.
The oscillations of a single leaf are expected to be larger than the oscillations of a
whole plant. Light-Driven oscillations affect more higher leaves. The whole plant
is less affected due to shading of leaves. The following chart consists of a
smoothed (30 minutes moving average) weight scale experiment and a
simultaneous gas exchange measurement.
Chart 1: Simultaneous measurement of plant transpiration: Gas exchange –
and scale.
From Maters thesis Leonid Asipov, 2011.
Fig 5:
37
1: Light (ORANGE)
2: Transpiration of a leaf(BLUE)
3. Transpiration of whole plant(DARK BLUE)
Light: SUNLIGHT. LONG TERM FLACTUATIONS ARE CLEAR IN GAS
EXCHANGE AND SCALES.
Short term oscillations are random and night/day unspecific. Longer oscillations
(0.5 hour) are related to fluctuations of irradiation levels. The pattern of the
correlation is seen both in gas-exchange and scales experiment. Longer term
oscillations are related to irradiation fluctuations. The short term oscillations seen
on electronic scales measurement, are related to weight measurement inaccuracy.
We would expect that oscillations in transpiration rate should be larger at
daytime since transpiration is larger. The measured oscillations were found to be
similar during DAY/NIGHT,
suggesting the oscillations are not related to the transpiration rate but to
spontaneous measurement noise.
SHORT TERM OSCILLATIONS ARE ONLY SEEN AT ELECTRONIC
SCALES MEASUREMENT OF A WHOLE PLANT.
The significance of the measurement of the scales is when the transpiration is
larger significantly than the measurement noise.
The transpiration of 0.5 hour is 80 grams at maximum transpiration levels. The
noise is 0.3 % which is about +- 2 grams. 2 grams is 2.5% of the measurement.
The smaller the scale of consideration, the degree of noise is larger than the total
transpiration during the period of time. The transpiration during 3 minutes is 8
grams. 2 grams of 8 is 25%. The extent of the spontaneous oscillations is large
and the spontaneous noise on the scale of 3 minutes (the considered time scale of
the reported article) is similar to the reported oscillations.
Since the reported oscillations are similar to the spontaneous noise of the
electronic scale, short time periods are not to be considered significant to the
measurement. The measurement is considered to be significant when the extent
of the transpiration is significantly larger than the noise. The time scale to
38
consider is larger than 0.5 hour.
The total transpiration between the measurements is about 10 grams (3 minutes).
The spontaneous inaccuracy is +-2 grams per measurement. 2 grams is about
20% of the measurement and is in the size of the reported oscillations. The
weight is not to be considered on short time scale, when the total transpiration is
not significantly larger than the spontaneous noise. The suggested time scale is
not less than 0.5 hour, when the noise is about 2.5% of the total transpiration.
The short term fluctuations are artifact.
Fig 6:
Measurements each 10 sec, averaged to 3 minutes.
Y Axis: weight loss [grams/sec]
X Axis: Time of day
PURPLE: Transpiration without smoothing
GREEN: Smoothing 13 points
RED: Smoothing 25 points
"The term" short term oscillations are related to fluctuations of transpiration on
scale of less than 3 minutes. The short term oscillations are considered an artifact
of the electronic scale.
Transpiration and Photosynthesis measurement in controlled environment: stable
transpiration parameter, changes due to CO2 changes in the air.
39
GAS EXCHANGE METHOD SHOW NO SHORT TERM OSCILLATIONS IN
CONTROLLED ENVIRONMENT
1. Gas-exchange measurement of photosynthesis and transpiration in controlled
environment: no short term oscillations, transpiration responds to changes in
CO2.
Fig 7:
1: orange Light levels.
2. Purple CO2 Concentration
3. Green Photosynthesis
4. Blue Transpiration of a tomato plant is controlled room.
Measured by gas-exchange method.
41
Fig 8:
Gas-exchange measurement of photosynthesis and transpiration in controlled
environment: no short term oscillations, transpiration responds to changes in
CO2.
Similar experiment
Gas exchange method works on a single leaf. The speculated oscillations should
have been larger since synchronization of oscillations is hard to imagine.
Nevertheless, no short term oscillations in plant transpiration are evident in gas-
exchange measurement of transpiration.
CONCLUSION: THERE ARE NO SHORT TERM OSCILLATIONS IN
PLANT TRANSPIRATION
L-Data vs “The Role of Tobacco Aquaporin1 in Improving Water Use
Efficiency, Hydraulic Conductivity, and Yield Production Under Salt
Stress1”
11/15/2012 11:34:24 AM
41
Rationale:
Expression of an additional aquaporin improves water transport of the plant.
Improvement of the water transport elevates the transpiration levels. The
photosynthesis levels might elevate a little, however the main effect of stomata
are on transpiration.
Fig 1:
1: light levels
2. CO2 concentration
3. Photosynthesis
4. Transpiration
Gas exchange experiment in controlled environment. Tomato photosynthesis and
Transpiration rates during changing CO2 concentrations.
Result
1. Fast acclimation of photosynthesis to CO2 alterations and constant
photosynthesis rate during the stomata aperture change.
Conclusion
42
1. Stomata barely affect photosynthesis.
2. The negative effect of salt on photosynthesis is not result of stomata closure
(which does not change the abundant CO2 concentration), but due to the direct
effect of salt on the plant photosynthesizing enzymes.
3. Enhanced water transport may improve enzymatic rates ONLY at salt stress
conditions. The more open stomata due
to the expresses aquaporin, are supposed to enhance transpiration but not elevate
photosynthesis at normal (NOT SALT) conditions, since stomata aperture is not
related to the photosynthesis rate(Fig1).
The reported WUE enhancement of the transgenic plants is absurd,
Water use efficiency: Total dry plant to unit of water weight.
Plant dry weight [kg] /WATER [kg]
The transpiration rate is about 70 times faster (per mole water relative to mole
CO2) than photosynthesis.
In order to preserve constant WUE at elevation of 15% in transpiration levels,
which is seen in the transgenic plants, Photosynthesis has to be elevated 10 times
(1000%) 70 + 15% of 70 = 80 photosynthesis units.
The molar weight of water is 19 and the assimilation of a single C atom is only 6
gram/mole. The O2 is returned back to the air and thus, at single molar
measurement of photosynthesis and transpiration additional 300% of the
elevation in transpiration rate to be added to the photosynthesis rate in order to
preserve constant WUE.
70 + 15% of 70 + 3* 15% of 70 = number of photosynthesis units needed for
CONSTANT WUE = 42 times more photosynthesis needed to preserve constant
WUE at 15 % more transpiration.
The duration of transpiration is the whole day, while the photosynthesis is
abundant only at day time. Therefore the total daily transpiration of the
transgenic plants is elevated MUCH more than the total photosynthesis.
43
The reports regarding the elevated WATER use efficiency of the transgenic
plants are not logical.
Every percent of elevation in transpiration levels is actually more than 3 times
more water per unit of dry weight.
The amount of CO2 molecules per single water is 70, meaning that elevation in
transpiration is serious to WUE.
For a single percent of added transpiration 300% of photosynthesis is needed to
keep constant WUE, otherwise
WUE declines.
The cells which are full in water are larger in size, and thus the time CO2
diffuses is longer. The longer time of diffusion causes usually decline in
photosynthesis rates in highly irrigated plants.
The extent of photosynthesis addition of the transgenic plants is incomparable to
the extent of used water.
The improvement of 50% in photosynthesis is only in peak irradiation hours and
salt stressed plants.
To elevate WUE photosynthesis has to elevate 45 and not 2 times.
NO ELEVATION IN WATER USE EFFICIENCY IS EVIDENT EVEN IN
THE TOP LIGHT and SALT STRESS CONDITIONS, when the aquaporin is
the most affecting the plant.
During normal conditions the differences in photosynthesis are not large,
however the difference in transpiration in steady on ~16% from wt. Note that the
plant transpires all the day and not only at peak light hours.
Inconsistency of the results measured by gas-exchange and whole plant scale
measurement.
Figure 3 presents larger transpiration rates to transgenic plants both at salt and
normal irrigation. Figure 2 presents transpiration results of whole plant. The
whole plant transpiration of transgenic plants is lower than WT. Aquaporins
44
improve water transport, and thus more transpiration is expected.
HOW THE AUTHOR EXPLAINS THE INCONSISTENCY?
WHY IS PHOTOSYTHESIS ON NORMAL IRRIGATION IS NOT
PRESENTED?
The effect of the aquaporin is significant only when there is water deficit. At
normal irrigation, the photosynthesis levels of Transgenic and wt plants should
not differ significantly. The reason of the aquaporin effect on photosynthesis is
that the photosynthesis is reduced by high salt concentration during salt stress.
Moreover, the transgenic plants transpire more water and thus get faster to
drought stress. The addition of the aquaporin enhances water transport, which is
positively affecting photosynthesis only at salt stress conditions, when the salt
levels start to block enzymatic processes.
CONSTANT LIGHT DURING THE GAS-EXCHANGE EXPERIMENT
The light during the experiment was artificial and constant. The reason for high
transpiration at noon hours is temperature not irradiation.
Due to the artificial light, the photosynthesis was elevated during the whole
measurement period (morning=>noon), which would have not been expected
with natural light, which is at PEAK only few hours a day. When light is less
intense, differences in photosynthesis rates are smaller.
The LIGHT levels was 1200uE which is stronger intensity relative to real
conditions. The light was TOO high in order to intensify the photosynthesis rates
of plants and enhance the effect of the aquaporin on salt stress. LESS LIGHT
would have declined the TOTAL photosynthesis and thus WUE would have been
declined.
TOO HIGH LIGHT is UNHONEST EXPERIMENT.
The real light is lower, not only to the top leaves but to the rest of the plant. Most
of the plant is at much lower light levels (~200uE).
The differences in photosynthesis rates due to better water transport during salt
stress would have declined to ~5-10 % at maximum relative to the reported 50%.
45
Note that the transpiration of the transgenic plants is elevated at all conditions to
more than 15%. At real conditions, the light levels are much lower, regarding the
fact that at morning and evening hours the light is very low intensity.
However, transpiration levels are related to temperature and are not maximum,
since the temperature of the air is related to the real irradiation.
CONSTANT LIGHT DURING EXPERIMENT IS A FRAUD. THE
TEMPERATURE OF THE AIR ALTERS TRANSPIRATION,
BUT PHOTOSYNTHESIS IS KEPT MUCH MORE CONSTANT DUE TO
CONSTANT LIGHT (NOT ENTIRELY CONSTANT DUE TO
TEMERATURE WHICH INCREASES THE PHOTOSYNTHETIC
REACTIONS AND DIFFUSION).
THE PHOTOSYNTHESIS CURVE WAS NOT PRESENTED MOST
POSSIBLY DUE TO CONSTANT LIGHT WHICH DOES NOT
CORRELATE
TO THE CHANGING TRANSPIRATION.
NATURAL LIGHT DID NOT AFFECT PHOTOSYNTHESIS, ONLY
TRANSPIRATION.
46
Fig 5: Typical irradiation levels in a greenhouse with natural light. UPPER
LEAVES. Rest of the plant, LESS than the following. Average irradiation of
morning: 450-500 uE, average irradiation of Noon : 750uE
The reported experiment : constant 1200uE from morning to evening.
The publisher enhances photosynthesis, which is enhanced by the aquaporin at
SALT STRESS.
At real light conditions there is less photosynthesis due to lower light levels.
The transpiration levels, however are kept according to the air temperature and
are influenced by the changing irradiation levels.
The real light is HOT, and the artificial light is cold.
1200uE of SUNLIGHT would have elevated transpiration significantly. 1200uE
of artificial light, is constant,
from morning to noon and transpiration is related to air temperature affecting on
the whole plant.
The effect of irradiation on transpiration is reduced due to the cold artificial light
of the measurement chamber.
The researcher accents photosynthesis and tries to hide transpiration. In order to
47
report POSITIVE WUE of transgenic plants.
HOWEVER TO IMPROVE WUE, photosynthesis has to rise by 45 times of the
elevation of transpiration, during the whole day
Period due to mass differences of water and Carbon and molar differences of
transpiration and photosynthesis.
The SALT STRESS EXPERIMENT
Salt ACCUMULATION in plant force plants to grow BAD on water with high
salinity. The more plants transpire, faster the accumulation of salt, and therefore
the plants live shorter lives until the plant death. Growth and fruit count declines.
The transgenic plants are more sensitive to salt on the longer run due to salt
accumulation.
The addition of salt during the experiment is not a good model for NATURAL
field conditions. The fields which absorb too much salt for plant growth should
be replaced with new soil. Drought stress is the real experiment to test new
genetically engineered plants. The drought stress is predicted to be a failure for
the transgenic plants carrying the aquaporin which causes more transpiration and
thus the plants get stressed before the wt plants. Elevated transpiration of the
transgenic plants DECLINE WUE causing drought sensitive plants or LARGER
WATER DEMAND.
The improvement of photosynthesis is ONLY at SALT STRESS CONDITIONS
WHICH ARE NOT REAL.
BETTER SOLUTION THAN GENETIC ENGINEERING IS REPLACING
THE SOIL.
Conclusions
1. WUE of the transgenic plants to decline since the differences in photosynthesis
rates are not high enough relative to differences in transpiration.
2. The transpiration of the whole plant is inconsistent with transpiration of a
single leaf (ERROR IN FIGURE?).
48
3. The effect of stomata aperture on photosynthesis is small. The effect of the
aquaporin enhances the photosynthesis mainly at salt stress conditions, when
enzymes has not optimal concentration of solutes. Better water transport elevate
photosynthesis. The transpiration is mostly related to stomata aperture. The effect
of the water on photosynthesis is not related to stomata, otherwise we would
expect similar or smaller effect on photosynthesis.
4. The transgenic plants are salt stress sensitive due to salt accumulation.
5. The transgenic plants are drought stress sensitive due to higher transpiration
rates.
6. The LIGHT levels during the gas-exchange experiment was constant, larger
and colder than natural light. The fact enhances photosynthesis, while
transpiration is reduced. SUNLIGHT is hot irradiation which enhances
transpiration.
The elevation of transpiration during the day is due to temperature changes in the
greenhouse.
The constant light is UNHONEST SCIENCE, since the air temperature was not
kept constant (SEEN AS UNCONSTANT TRANSPIRATION LEVELS).
The reason for the lack of photosynthesis after dehydration is lack of water
for enzymes
30/12/2012 18:51:51
Transpiration and photosynthesis during three dehydration days. Note that day 2
is less water for plants and nevertheless there is more transpiration and same
photosynthesis. The stomata close. Same photosynthesis : there is no relation of
stomata and photosynthesis: enough water = same photosynthesis.
Transpiration is higher due to higher leaf temperature and less water elevation by
the roots thus less leaf cooing by transpiration...
49
WUE CANNOT GROW
10/9/2012 11:57:08 AM
CRITICISM ON : "The role of tobacco aquaporin 1 in improving water-use
efficiency, hydraulic conductivity and yield production under salt stresses. "
WUE OF PLANTS EXRESSING AQUAPORIN CANNOT GROW. MORE
WATER FOR TRANSPIRATION ALL THE TIME. PHOTOSYNTHESIS HAS
TO DECLINE DUE TO MORE WATER IN CYTOPLASM. MOR DIFFUSION
TIME. THE OPEN STOMATA ARE NOT SUPPOSED TO ENHANCE
PHOTOSYNTHESIS DUE TO HIGH SPEED OF DIFFUSION OF CO2.
NEVERTHELESS, THE PERIOD OF LIGHT IS SHORT AND THE
TRANSPIRATION ELEVATION IS FOR LONG TIME. THE TOTAL WUE
HAS TO DECLINE DUE TO TRANSPIRATION ELEVATION. THE
RESULTS SUGGEST ELEVATION OF WUE but ONLY FOR A TOP LIGHT
STRENGTH AND SHORT PERIOD (GAS EXCHANGE, ARTIFICIAL LIGHT
AND TOP CO2 CONCENTRATIONS).
L-Data vs "SYNERGISM OF LIGHT AND FUSICOCCIN ON
STOMATAL OPENING"
51
11/15/2012 12:03:31 PM
Stomatal opening in FC or KCI.
How possible that higher concentration of KCL open the
stomata and not closing? High concentration, water exits the stomata and the
stomata closes.
2. Why to couple 2 effects: solute concentration and light?
Light causes photosynthesis and thus decline in CO2 concentration.
When CO2 concentration is below threshold of stomata opening, the stomata
start to open.
Solute concentration is effect unrelated to light.
L-Data vs "Changes in the properties of reaction center II during the initial
stages of photo inhibition as revealed by thermo luminescence
measurements"
11/15/2012 11:56:53 AM
Leaves of plants exposed to intense light accumulate more light particles. At
lower temperatures than usual, the particles will be depleted off the plant tissue.
The switch of the band from higher temperature to 15% is due to more light
particles accumulated in the leaf.
Phosphorus is the main matter accumulating light and producing
phosphorylation. The concentration of phosphorus is the main factor of
phosphorylation, as measured by TL system. The TL is mainly a phosphorus
meter. Additional light absorbing substances are also existent.
A regular spectrophotometer can usually replace TL.
Photo inhibition is due to destruction of plant leaves by excessive irradiation. The
cure: shading.
CO2 not light is the trigger for leaf conductance to gases
51
1/9/2011 11:16:47 AM
Introduction
Changes in light levels or air CO2 concentration cause changes in leaf
conductance to gases.
I: Leaf conductance changes in response to changes in air CO2 concentration :
the response happens
in darkness or light.
Conclusion
The response ton changes in CO2 concentration is light independent. This
suggests that CO2 is a trigger which inflicts on leaf conductance to gases. In light
the effect is stronger due to photosynthesis, which lowers the CO2 concentration
near and inside the leaf.
II: Direct effect of light
1. High Light
52
2. Low light
Conclusions
The effect of light on leaf conductance (which we see as transpiration rate) after
turnover from darkness is not immediate, and gets larger with lower light levels.
During this response time, the COI2 levels in the leaf intercellular space gets
below certain threshold after which an effect on transpiration is visible. In higher
53
light levels thr transpiration response time shortens significantly as intercellular
CO2 threshold is reached quicker in faster photosynthesis levels. The fact that the
effect of light on leaf conductance to gases (as seen in transpiration) is not
immediate and related to the gradual reduction in the CO2 concentration in the
intercellular space, in addition to light independent response of the leaf
conductance to gases to changes in CO2 concentration in the air, suggests that
CO2 is the primary trigger of the leaf conductance to gases, while light plays an
indirect role as Photosynthesis inducer.
Materials and Methods
Data-Lightning universal platform for data analysis, L-Data.
There is no main growth control in plant : transpiration is related to
nutrient transport
8/28/2011 9:41:40 AM
‫של‬ ‫אין‬
)‫חלבונים‬ ,‫(פחמימות‬ ‫בנייה‬ ‫חומרי‬ ‫של‬ ‫מדפוזיה‬ ‫נובעת‬ ‫שרואים‬ ‫התופעה‬ .‫בצמח‬ ‫קודקודי‬ ‫טון‬
‫טרנספירציה‬ ‫גם‬ ‫ולכן‬ ‫פוטוסינטזה‬ ‫שמבצעים‬ ‫בצמח‬ ‫לעלים‬
.
‫המים‬ ‫של‬ ‫הריכוזים‬ ‫מפל‬ ‫עם‬ ‫תמידית‬ ‫מים‬ ‫של‬ ‫זרימה‬ ‫יש‬ ‫טרנספירציה‬ ‫כשיש‬
.
‫מורידה‬ ‫טרנספירציה‬
‫לעלה‬ ‫מים‬ ‫של‬ ‫זרימה‬ ‫יש‬ ‫ולכן‬ ‫המןמסים‬ ‫ריכוז‬ ‫את‬
.
‫לאט‬ ‫לאט‬ .‫לעלה‬ ‫מים‬ ‫של‬ ‫זרימה‬ ‫אין‬ ‫ולכן‬ ‫טרנספירציה‬ ‫ללא‬ ‫נמצאים‬ ‫פוטוסינטזה‬ ‫מבצעים‬ ‫שלא‬ ‫עלים‬
‫מדי‬ ‫איטית‬ ‫הסוכרים‬ ‫ואספקת‬ ‫הסוכרים‬ ‫נגמרים‬
.
‫אספקה‬ ‫מהעדר‬ ‫נושרים‬ ‫בחושך‬ ‫ושנמצאים‬ ‫פוטוסינטזה‬ ‫ללא‬ ‫העלים‬
.
‫תאים‬ ‫של‬ ‫למוות‬ ‫גורמים‬ ‫סוכרים‬ ‫עודף‬ .‫סוכרים‬ ‫מעודף‬ ‫נובלים‬ ‫שנתיים‬ ‫חש‬ ‫צמחים‬
‫מאוסמולריות‬
‫מהתייבשות‬ ‫מת‬ ‫והצמח‬ ‫גבוהה‬
.
‫בעתיד‬ ‫משתלמת‬ ‫אבל‬ ‫אנרגטית‬ ‫השקעה‬ ‫שעולה‬ ‫וגדילה‬ ‫אנרגייה‬ ‫ייצירת‬ ‫הם‬ ‫צמח‬ ‫של‬ ‫חיים‬ ‫תחילת‬
.
‫מנת‬ ‫על‬ ‫מספיק‬ ‫מייצר‬ ‫לא‬ ‫עצמו‬ ‫העלה‬ ‫כי‬ ‫לגדילה‬ ‫אספקה‬ ‫מקבלים‬ ‫באור‬ ‫שנהנצאים‬ ‫וענפים‬ ‫עלים‬ ‫רק‬
‫הצמח‬ ‫כל‬ ‫של‬ ‫משותפים‬ ‫ממשאבים‬ ‫הגדילה‬ ,‫לגדול‬
.
‫רי‬ ‫כשיש‬
‫הפחמימות‬ ‫ריכוז‬ ‫את‬ ‫לווסת‬ ‫מנת‬ ‫על‬ ‫לגדול‬ ‫רוצים‬ ‫עלים‬ / ‫הענפים‬ ‫כל‬ ‫אנרגייה‬ ‫של‬ ‫גבוה‬ ‫כוז‬
.
‫האנרגייה‬ ‫את‬ ‫משקיע‬ ‫הצמח‬ .‫פירות‬ ‫גידול‬ ‫מתחיל‬ ,‫מסויים‬ ‫ריכוז‬ ‫מעל‬ ‫גדל‬ ‫הפחמימות‬ ‫שריכוז‬ ‫בשלב‬
‫ולא‬ ‫קבוע‬ ‫ם‬ ‫מומסי‬ ‫ריכוז‬ ‫על‬ ‫לשמור‬ ‫מנצת‬ ‫על‬ ‫בפירות‬
‫שפירות‬ ‫בסוף‬ .‫לסף‬ ‫מעבר‬
-
‫אפשרות‬ ‫אין‬ ‫גדולים‬
‫האנרגייה‬ ‫וריכוז‬ ‫אנרגטית‬ ‫להשקעה‬ ‫לצמח‬ ‫אחרת‬
‫המקסימלי‬ ‫לסף‬ ‫מעבר‬ ‫עולה‬ ‫בצמח‬
54
‫הצמח‬ ‫והתיישבושת‬ ‫תאים‬ ‫של‬ ‫למוות‬ ‫שגורם‬ ‫מה‬
.
‫נוע‬ ‫הדבר‬ .‫הימוח‬ ‫אנרגיית‬ ‫את‬ ‫מקבלים‬ ‫העליונים‬ ‫והענפים‬ ‫שהעלים‬ ‫בכך‬ ‫מתבטא‬ ‫הקודקודי‬ ‫השלטון‬
‫טרנספירציה‬ ‫ומבצעים‬ ‫באור‬ ‫המצאים‬ ‫שהם‬ ‫מכך‬
.
‫יק‬ ‫גורמת‬ ‫טרנספירציה‬
.‫לצימוח‬ ‫שטובים‬ ‫והחלבונים‬ ‫הסוכרים‬ ‫לאספקת‬ ‫ולכן‬ ‫המים‬ ‫בפוטנציאל‬ ‫רידה‬
‫קודקודי‬ ‫שלטון‬ ‫של‬ ‫נוספת‬ ‫תופעה‬
-
‫אם‬ ‫הציד‬ ‫גידול‬
‫ראשית‬ ‫מריסטמה‬ ‫להעדר‬ ‫קשורה‬ ‫התופעה‬ .‫קודקוד‬ ‫אין‬
-
‫מנוצלת‬ ‫האנרגייה‬ .‫צימוח‬ ‫אפשרות‬ ‫אין‬
‫אחרות‬ ‫במריסטמות‬
.
‫טרנספירציה‬ ‫פחות‬ ‫צריך‬ ‫כך‬ ,‫אנרגייה‬ ‫יותר‬ ‫שיש‬ ‫ככל‬
‫אספקה‬ ‫לקבלת‬
,
‫שעשיר‬ ‫צמח‬ ‫של‬ ‫במצב‬ ‫כך‬
‫באנרגייה‬
-
‫הצידה‬ ‫גם‬ ‫היא‬ ‫הגדילה‬
.
Cactuses create sugar during the day
9/8/2011 3:39:39 PM
55
Cactuses create sugars from CO2 and water. During the night, all the gas
exchange takes place. The stomata open, the O2 from the day irradiation diffuses
to the air and CO2 diffuses to the plant. The plant structure is low surface
area/volume ratio and accumulates large amount of CO2 and O2. A the morning
stomata close and irradiation start creating O, H and C from H2O and CO2. The
O2 is accumulated in the plant leaves, and CO2 is the accumulated CO2. The
sugar is enzymatically formed during the day, while the basic components are
created. As soon as stomata are opened during the night, gas exchange takes
place. CO2 diffuses to the plant, where CO2 concentration is low. O2 diffuses to
the air. Without irradiation, the basic components are becoming back to CO2 and
H2O. During the night only gas exchange takes place.
There is no CO2 fixation : O2 concentration blocks photosynthesis by
reverse CO2 formation
56
9/11/2011 6:54:38 PM
There is no CO2 fixation. CO2 diffusion from the air
brings CO2 to cells, then light break the two bonds of
CO2 and leaves C in the cells. O2 is diffused back to air.
The effect of O2 on photosynthesis is C returning back to
O2 and CO2 reforming. On higher O2 concentrations, the reaction
of C creation is reversed, and thus, no visible photosynthesis
takes place (photosynthesis is measured by CO2 concentration change in time
unit).
The formation of C from CO2 is light driven, not enzyme driven.
The next stage is enzymatic formation of sugar.
Stomata don't affect photosynthesis
9/18/2011 9:59:52 AM
Stomata aperture don't affect photosynthesis, only transpiration.
Decoupling of photosynthesis and transpiration rate after CO2 changes,
has to be explained. The difference of the effect is due to high water
concentration
difference between the plant and the air.
CO2 concentration difference is not high, due to respiration of the plant.
The stomata aperture mainly affect not photosynthesis.
57
CO2 Channels Have to Enlarge Transpiration
9/25/2011 4:19:05 PM
CO2 channels have to enlarge transpiration. Any channel which conduct
molecules
with size larger than water, would conduct water. If Such channels exist, they
would
have to conduct water. Over expression of CO2 channels would enlarge
transpiration.
CO2 channels are static like every channel. Since no charge exist, the channels
are
only size limiting. Whether if they exist we don't know. An possibility is that
water
channels conduct CO2 also, they have a size larger than water molecule.
The stated protein was said to lower transpiration. If the protein, as stated is a
CO2 channel, transpiration would have to be elevated.
58
That is the reason for absence of any physiological effect of stated proteins
on photosynthesis or transpiration.
Human industry is nature's best friend
10/23/2011 11:04:39 AM
Humans are friends of nature. Without humans, nature would have
run off of CO2. Bacreria which create oil, take CO2 and create solid
or liquid matter. CO2 is off circulation. Humans found oil, and coal
and crated industry and engines, while returning CO2 back to
atmosphere. With no humans nature would have disappeared, plants would have
no
CO2 to produce sugars and animals would have no food.
CO2 circulation due to industry elevate crops and saves water.
Humans and modern culture of industry is best friend of nature.
No need for CO2 channel for chloroplast
1/18/2012 9:19:29 AM
59
L-Data vs Rapid assessing of water and nitrogen status in wheat flag leaves.
NOT N, possibly only water is reason for difference in weight of wheat
10/10/2012 3:00:42 PM
"Near pipes there is more water and N. Weight of wheat is higher. The authors
wrote N is causing the weight. WHY NOT IRRIGATION? TRAIT OF WEIGHT
ON FIELD PIPE.AND
Physics
Light Speed is not Constant
Light speed is not constant. Light particles have mass, and like everything with
mass
61
are subject to gravity interactions with other masses. The light particles are
emitted
from the sun in process of thermonuclear processes and have high speed and high
energy (much higher than visible light). In the process of light travel in space
there
is constant gravitational force of the sun on the light particles, which slow down
and
with their arrival to earth they are less energetic and mostly in visible light
spectrum.
The light rays are not straight too, due to mass effect by planets from the side
during their
travel in space.
Relativity Theory Wrong
5/11/2011 7:12:57 PM
Relativity theory wrong:
Matter is not relative. Gravitational interactions between masses
61
exist without dependence of other factors.
The only two natures exist in the world is mass (matter) and its movement.
Relative phenomena is movement of masses. Speed is rate of change of position
distance/per time = movement of mass relative to another mass. But not matter
itself: it is not relative.
‫משוגע‬ ‫היה‬ ‫ידוע‬ ‫מדען‬
‫יחסי‬ ‫אינו‬ ‫החומר‬
‫תנוע‬ = ‫והאנרגייה‬
‫חומר‬ ‫של‬ ‫ה‬
‫מחומר‬ ‫תנועה‬ ‫ליצור‬ ‫ניתן‬ ‫כביכול‬ ‫שלפיה‬ ‫דבילית‬ ‫נוסחא‬ ‫כובת‬ ‫המדען‬
.
‫הידועה‬ ‫הנוסחא‬
=
‫בטלוויזיה‬ ‫שודר‬ ‫אפילו‬ ‫שלאחרונה‬ ‫דביליזם‬
.
‫לעבור‬ ‫צריך‬ ‫שחומר‬ ‫לכך‬ ‫נגרר‬ ‫יחסי‬ ‫שהכל‬ ‫התחלתית‬ ‫הכרזה‬ ‫על‬ ‫מגונן‬ ‫בעודו‬ ‫המדען‬
= ‫ותנועה‬ ‫חומר‬ .‫נפל‬ ‫המדען‬ ‫למעשה‬ .‫המדען‬ ‫נופל‬ ‫אחרת‬ ‫לתנועה‬
‫שונים‬ ‫מושגים‬ ‫שני‬
.
Matter does not have inner structure
5/16/2011 5:25:55 PM
Matter does not have an inner structure: inner structure was
historically to explain magnetic and electrostatic forces.
No inner structure is better perspective: all the particles have affinity
to one another. Electrostatic forces are due to the effect of elevation in
gravitational force in smaller masses due to surface area/volume ratio.
The smaller particles have higher affinity and thus electrostatic forces are
created. Magnetic force is due to spontaneous exit of particles from the
conductor.
There is no minimum speed needed to fly to space
8/17/2011 1:54:32 PM
‫מהי‬ ‫שצריך‬ ‫טענו‬ ‫מדענים‬
‫לאורביט‬ ‫מסה‬ ‫ליציאת‬ ‫מינימלית‬ ‫רות‬
‫ה‬
.
‫דלק‬ ‫מספיק‬ ‫שיש‬ ‫בתנאי‬ ‫איטית‬ ‫במהירות‬ ‫אפילו‬ ,‫הארץ‬ ‫כדור‬ ‫של‬ ‫המשיכה‬ ‫מכוח‬ ‫לצאת‬ ‫ניתן‬
.
‫הדלק‬ ‫תצרוכות‬ ‫עולים‬ ‫ולכן‬ ‫המשיכה‬ ‫כוח‬ ‫מול‬ ‫להחזיק‬ ‫זמן‬ ‫יותר‬ ‫בהרבה‬ ‫צורך‬ ‫יש‬ ‫איטית‬ ‫במהירות‬
‫הרבה‬ ‫ששוקל‬
.
,‫קל‬ ‫דלק‬ ‫עם‬ ‫מנוע‬ ‫ויש‬ ‫במידה‬
‫מהירות‬ ‫בכל‬ ‫לחלל‬ ‫לטוס‬ ‫ניתן‬
.
‫לכדור‬ ‫המסה‬ ‫של‬ ‫לחזרה‬ ‫תגרום‬ ‫מספיקה‬ ‫לא‬ ‫מהירות‬ .‫הארץ‬ ‫לכדור‬ ‫במשיק‬ ‫מהירות‬ ‫דורשת‬ ‫אורביטה‬
‫הארץ‬
,
62
‫הגורם‬ .‫נדרשת‬ ‫מינימלית‬ ‫מהירות‬ ‫ואין‬ ,‫המשיכה‬ ‫מכוח‬ ‫ולצאת‬ ‫לחלל‬ ‫לטוס‬ ‫ניתן‬ ‫מהירות‬ ‫בכל‬ ‫אבל‬
‫דלק‬ ‫הוא‬ ‫המגביל‬
‫בצורה‬ ‫הסבירות‬ ‫לא‬ ‫המדענים‬ ‫וכך‬ ‫המהירת‬ ‫לא‬
‫של‬ ‫המשיכה‬ ‫מכוח‬ ‫יציאה‬ ‫של‬ ‫ט‬ ‫הקונצפ‬ ‫את‬ ‫נכונה‬
‫הארץ‬ ‫כדור‬
.
Gravitational force is related to Surface Area/Mass ratio
8/21/2011 2:34:33 PM
Gravitation of small particles
Gravitational force has direct relation with mass surface area/volume proportion.
Force create acceleration, which is directly related to mass. The surface area
is the area on which a collision with the gravitation particle can happen.
Thus the relation of the gravitation force has to be
F= SurfaceArea/Mass.
Smaller masses have relatively higher gravitational effect but only on
collision with gravitation particles. Without collision, they travel farther,
63
due to lower collision chance.
Irradiation consists of large amount of particles and thus many of them travel
without any collision. The more surface area a mass has, the more chance is to
collide with the
gravitation particle. And thus, larger masses have larger visible effect of
gravitation.
High speed of particles with large mass can make collision with gravitation
particles not to be in high effect.
If masses relatively larger than a single gravitation particle are considered,
the frequency of collisions with the gravitation particle is higher to mass
unit in masses with high surface area/ mass ratio relative to masses with lower
surface area/mass ratio.
Smaller masses have higher gravitational forces with proportion to the mass.
This is correct to relatively large particles, such as atoms. Smaller particles
such as photons, electrons have lower chance for collision with gravitation
particles and therefore the effect of gravitation is less than for larger particles.
This is seen is large distance travel of photons, and wire skipping of electrons
following electric force.
The particles are not of right form. Sand, salt and any other materials
8/23/2011 12:52:48 PM
The particles are not of right form. Sand, salt and any other materials
under the microscope show differences in particle size,
form and generally without proper form.
We speculate that the phenomena is also on microscopic level of atoms.
They vary in size, mass, their properties are due to differential
concentration and therefore hardness which is related to the time the matter
was concentrating by the gravitational forces.
The world is not consisting of even size or right form particles.
All the particles are different, and the distances between them are various.
The gravitational force concentrate the particles and gradually change
64
their form until gaps disappear.
There is no possibility of creating ideal
space
8/28/2011 10:05:49 AM
There is no possibility of creating ideal space. Every space is particle collection.
To create space, a flow of smaller particles is sent through an area with larger
particles.
The smaller particles are pushing the larger ones off an are but themselves are
spread
in the area. During the whole time of the flow of the small particles some
particles flow in
opposite direction.
65
Classical energy equation is wrong : energy is speed/mass
9/11/2011 6:46:20 PM
Energy is speed/mass, not the usual equation.
All there is is movement to mass. The classic equations
are wrong. In mechanical system, movement/mass is constant.
Masses transfer movement/mass to each other at collision.
Since all there is is matter and speed, speed/mass
is the only possible energy parameter.
66
The parameter which change matter properties
is mass/volume, matter concentration.
Movement of masses is enough to explain all physical or chemical
phenomena
8/21/2011 12:53:25 PM
There is two natures in the universe: mass, which is matter,
and it's movement, which is speed. On micro level small particles of matter,
without any further structure construct all the universe we see, including light,
air, water, hard material or magnetic fields.
The nature of forces: Gravitational force
67
The nature of forces between the particles is constant flux of tiny particles from
many directions on every mass. The collision of small particles and the other
masses create the gravitational force on every mass.
Small or big masses are affected by the gravitational particle flux.
A field is a cloud of particles moving in certain speed. In the area of the field
masses are affected by collisions of the particles of the field.
Gravitational particles create gravitational field. The force is not related
directly with the masses, it is caused by flux of tiny particles from the
outer side of the masses.
Magnetic Fields.
The nature of magnetic fields is flux of electrons from the wire.
Some electrons exit the wire from the side, the gravitation force affect the
particle from the side until the direction is around the wire.
68
The magnetic force is created by collision of electrons with the mass
affected by the force.
Activation energy: chemical bonds, Temperature, Exothermic and
Endothermic reactions
The effect of temperature is related to frequency and
power of collisions of particles on a surface. Chemical reactions require
energy which is minimal speed of particles needed for proper collision and
chemical bond creation. Less than the minimum speed won't stand against
the gravitational force and the back flow of gravitation particles
from every atom. Proper collision partially disrupt the structure of the
atoms creating tiny particle cloud around the area of the collision.
The gravitation particle flux concentrate the cloud until creation
of strong area which is called chemical bond.
On collision, the two particles lose speed and therefore the reaction
causes a temperature decline, due to reduction of the frequency
of collision of the particles on the surroundings.
Exothermic reaction is reaction with elevates the temperature
69
of the surroundings. This kind of reaction is disruption of chemical bonds.
The activation energy needed for exothermic reaction is movement of
other particles which have to collide with the bigger molecule until
disruption of the chemical bond. On disruption of the chemical bond,
smaller masses are created, which hit the surroundings with higher frequency.
The higher frequency cause temperature elevation, or better conduction of kinetic
energy from the atoms of the reaction to the surroundings.
Atomic Bomb
There is no ideological difference between the atomic bomb
and a regular fire. In fire a molecule of some fuel is disassembled to atoms
and the atoms collide with the bigger molecule. The collision create high
speed movement of the small masses as well as evaporation of the matter.
The high temperature is due to the high speed movement of the particles.
Atomic bomb is the same process on the level of the atom.
Uranuim atom is being partially destructed by a regular explosive.
The tiny particles created collide with the big atoms and travel on
71
high speed to every direction.
The particles create high temperature on collision with other masses.
Hydrogen Bomb
Hydrogen bomb is high speed movement of tiny particles created by
collision between two hydrogen atoms. The collision is caused with
collision of tiny particles created by an atomic bomb explosion.
The two hydrogen atoms collide and some of the particles of the
atom are released and collide with the bigger atoms.
On collision the tiny particles gain high speed. The tiny particles travel
on large distances because they are not collided with the gravitational particles.
They create high temperatures on collision with other matter.
71
Electrical force, structure of metals
Metals are constructed of atoms which are connected to each other.
The connection is uniform. Between the atoms of the metal small ducts are
created, in those ducts, electrons can travel from the high concentration to the
low
concentration, creating the possibility of electric power usage in their way.
The electrons as well heat the wire by random collisions. Some of the electrons
skip from the wire to the side, creating the magnetic field.
72
Gases, Liquids and Solids
The difference is in matter concentration. Gases are consisted of small
particles with large distances travelling on high speed. The particles can
be rearranged closer to each other by speed reduction or in other words,
temperature reduction. As soon as they are close, the gas is solid. The particles
of the gas are still to as concentrated as solids. Solids consist of small particles
which are very close to each other and thus the gravitational
force is affecting from all sides and keep the concentration of the mass
to maximum strength. The strength of matter is a sole result of closeness of the
particles to each other and the phenomena is seen in metals. It is enough to press
two pieces of metal to each other with strength if the surface is very straight.
The two pieces are connected strongly and act like connected material. Since
the atoms are with no right form (malformed structure) , it takes time to create
solid matter, since gravitational particles collisions create the effect
of matter solidification which is concentration of matter particles.
The structure of the particles is slightly rearranged and the spaces between
the particles disappear. As soon as this happed, the matter becomes strong.
Spaces between the matter particles make weaker matter.
Gases are matter with large spaces, liquids with smaller,
solids with even smaller. The smaller distances between the particles
of the solid, the stronger is the solid. Elevation of the speed of the particles
can make deconcentration of matter, liquidation , or evaporation.
Reduction of speed create more solid matter. The concentration
of matter is made by gravitation particles constant flux on every mass.
73
Electromagnetic fields
Electromagnetic field is a cloud of small particles travelling usually
in high speeds. Light particles travel in high speed and affect other
masses in the area of their travel.
Conclusions
The matter consists of tiny particles lacking any further structure.
The matter properties such as strength or matter state (liquid, gas, solid)
are a result of the distances between the matter particles.
The gravitational forces gradually create concentrated matter and the
matter structure becomes stronger.
All the forces (gravitation, magnetic forces, electrical forces) are a
result of collision of masses. Small masses which are gravitation particles
collide with all the other masses on frequent level.
These collisions create forces of affinity which lead to acceleration of the masses.
Phenomenae such as atomic bomb, thermo nuclear bomb are a result
of high speed collision of small or medium particles with Uranium/Hydrogen
atoms. The result is partial dissipation of the atom to smaller particles which
collide with the larger particle and thus travel in very high speed.
The tiny particles travelling in high speed are Irradiation resulting from
the reactions. The particles hit other matter, and create vibration / disruption
of molecules, elevating the temperature.
Regular combustion reaction is conceptually the same, with the
difference that the particles are not that small and the dissipated matter
is molecule and not atom. The resulting small particles are atoms,
which similarly to the tiny particles created in Nuclear/Thermo
nuclear reaction collide with larger particles and travel fast elevating
temperature. During the dissipation of molecules, the solid matter
becomes gaseous.
Magnetic forces have the same nature of result of mass collisions.
Electrons skip from the wire and due to wire deformation travel partially
74
sideways. The gravitational force affect the electrons to move mostly
around the wire.
Electrical forces are movement of electrons in ducts of the conductor.
Since the only observation is that mass collision cause speed change
of the two colliding particles can conceptually explain all the physical
and chemical phenomenae we conclude that the only natures in the
universe are matter and matter movement.
Thermodynamics are false
3/2/2011 11:31:59 AM
Thermodynamics are false. The degree of order cannot represent degree of
energy.
Energy is movement of matter. Out of order which is position of objects in space,
we cannot gain any movement. We move to create order and this movement is
energy
waste which cannot be regained.
‫החשמל‬ ‫חליקי‬ ‫ע"י‬ ‫חום‬ ‫ופיזור‬ ‫גדולים‬ ‫מוליכים‬ ‫של‬ ‫אלסטיות‬ : ‫חשמל‬
12/17/2012 11:26:49 AM
‫גוף‬ ‫או‬ ‫מנורה‬ .‫מכאנית‬ ‫לתנועה‬ ‫וגורמים‬ ‫המוליך‬ ‫על‬ ‫מתנגשים‬ ,‫החשמל‬ ‫של‬ ‫הקטנים‬ ‫החלקיקים‬
.‫ואלסטיות‬ ‫התייל‬ ‫של‬ ‫מתנועה‬ ‫אלא‬ ‫בעיקר‬ ‫מהחלקיקים‬ ‫לא‬ ‫החום‬ .‫אפשריות‬ ‫אפליקציות‬ ‫חימום‬
‫נע‬ ‫התייל‬
‫הקטנים‬ ‫החלקיקים‬ .‫מכאניות‬ ‫וויברציות‬ ‫קולות‬ ,‫האוויר‬ ‫טמפרטות‬ ‫בעקבות‬ ‫אלסטי‬ ‫באופןצ‬
‫לצרכן‬ ‫האוויר‬ ‫חלקיקי‬ ‫משאר‬ ‫שתנועתי‬ ‫מהתייל‬ ‫חום‬ ‫הסעת‬ ‫מאפשרים‬ ‫וכך‬ ‫חום‬ ‫הולכת‬ ‫מעלים‬
(
‫מנורה‬
)
.
Light Speed Changes with collisions
1/15/2012 1:31:51 PM
75
The speed of light changes with other light beams. The light is flux of particles.
Collisions change particle speed and thus light changes the speed. Flux of
particles in space is from many points and thus the speed of light declines. The
particles themselves are moving in different speed. The speed is seen as color in
visible light.
‫נכון‬ ‫לא‬ ‫הזרם‬ ‫מושג‬
2/21/2012 10:10:09 AM
76
RADIO WAVE DIFFERS SPEED NOT FREQUENCY
9/21/2012 11:12:50 AM
Irradiation= PARTICLES WHICH MOVE AT SOME SPEED AT A
FREQUENCY WHICH HOLDS THE SOUND INFO... SPEED=RADIO
STATION. THE WIRE IS BUILT ON A CIRCULAR MAGNET WHICH
DISTRACTS CERTAIN SPEED OF PARTICLES...A SINGLE SPEED IS
COLLIDING TO THE WIRE AND TRAVELS TO THE TRANSISTOR,
HEATING THE SILICONE, AND RESULTING Vibration OF LARGE
ELECTRIC FREQUENCY =>SPEAKER!!!!!!!!!
Light Speed is not uniform, WAVES WRONG...
Leonid Asipov
11/7/2012 10:27:24 AM
The property of color is related to the particle speed, and is function of
77
destructivity of the light flux. Destructivity is dissipation of speed to area. The
larger the speed, the smaller the particle, the destructivity is large. Slower and
larger particles are less destructive. The destructivity is barely rises with particle
density because the collisions with the target are diffused to the whole area of the
target. To a single unit of area (as large as a single light particle), only one light
particle can cause deformation, which is read and interpreted by the biological
organism as color.
The strength of light is correlative to the light density, density of the particles.
The frequency of the collisions with the target is correlated to the density of light.
Measuring light:
Measurement is a form of change in a measuring matter as a result of light flux.
The changes in the matter are dependent on the matter properties (such as
elasticity) as well as on the properties of the light flux.
Temperature cause electrical flux and by the addition of a diode, the electrical
flux is single directed and later is measured by addition of a magnet(after simple
amplification). The measurement is of temperature of a measuring matter. Since
matter has different heat flux properties such as different absorption of different
light speeds, the temperature is a qualitative parameter to compare different light
beams, and not to explain the properties of a single beam by itself.
After the measurement the temperature is written as light intensity. The intensity
changes according to the variation of the electricity which creates the artificial
light or the nature sunlight. The sunlight is created by explosions in the sun, and
thus the flux is of different density and particle speeds.
Additional way to measure light is by sound measurement. The particles are
hitting a small membrane which create electricity pulses. Tiny sensor (size of a
light particle) will theoretically return the real single light particle amplitude
(speed) and frequency (density of particles).
The light "wave": wrong parameter of wrong science.
The amplitude and phase are a way to track the real measurements and are not a
physical phenomena. Assume we are measuring light by a small sensor with a
size of a single light particle. The amplitude of a wave is correlated to the
strength of the collision of the particle with the sensor. The frequency is
78
correlated to the density of particles. The real sensors are larger than a single
light particle and thus many particles collide with sensor at a single time unit.
Therefore, the amplitude is correlated to speed and density of the light. The
frequency (phase of waves) is correlated to the change in intensity. Really, the
waves are changes in light intensity and average particle speed. Constant light
(constant density and average particle speed ) should return no waves at all. The
result is a single temperature VALUE without waves.
Electricity ERROR: Science fraud
79
11/29/2012 7:28:03 PM
The - electrode of the battery should hold electrons, according to the science.
However, the power is + electrode. Protons are not current. A simple connection
is + to electric device and the second wire has to be - or ground. The current is
from + to - . ELECTRONS ARE AT +!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
PIGMENT REFLECTION: ABSOBTION OF SPEED ...
8/5/2012 6:46:51 PM
Pigment does not reflect a color : it absorbs all colors and the properties of the
pigment reflect all particles to certain speed. The pigment does earn movement of
light collisions. The movement elastic properties of the pigment cause most of
the particles a reflection to a speed. A speed is related to visible color. ALL
LIGHT IS CAUSING THE VIBRATION OF THE LEAF. ALMOST ALL
LIGHT IS REFLECTED.
Chemistry
Periodic Table might be a falsie
5/19/2011 6:57:55 PM
‫כסטח‬ ‫הינה‬ ‫מחזורית‬ ‫טבלה‬
‫גז‬ ‫של‬ ‫מולקולות‬ ‫בין‬ ‫שמרחק‬ ‫בהנחה‬ ‫קבוע‬ ‫גז‬ ‫נפח‬ ‫בעזרת‬ ‫נמדדה‬ ‫מולרית‬ ‫מסה‬
-
‫קבועות‬
‫להי‬ ‫צפויים‬ ‫לא‬ ‫מרחקים‬ ‫ולכן‬ ‫מולקולות‬ ‫בין‬ ‫משיכה‬ ‫יש‬ ,‫אבל‬
‫זהים‬ ‫ות‬
.
‫חומר‬ ‫לכל‬ ‫שונה‬ ‫חלקיקים‬ ‫מספר‬ ‫ימצאו‬ ‫גז‬ ‫של‬ ‫אחיד‬ ‫בנפח‬ ‫לכן‬
.
‫בטבלא‬ ‫מסויים‬ ‫למקום‬ ‫ומסה‬ ‫מטען‬ ‫מבחינת‬ ‫התאימו‬ ‫שלא‬ ‫לאטומים‬ ‫ברשימות‬ ‫נוספו‬ ‫נייטרונים‬
.
‫אטומי‬ ‫למספר‬ ‫בנוסף‬ ,‫המדידות‬ ‫תוצאות‬ ‫עם‬ ‫המולרית‬ ‫המסה‬ ‫מסתדרת‬ ‫הנייטרונים‬ ‫הוספת‬ ‫עם‬
‫אחד‬ ‫בפרוטון‬ ‫תמיד‬ ‫שעולה‬
.
,‫למעשה‬
‫מסה‬ ‫ומבחינת‬ , ‫בטבלא‬ ‫משכתוב‬ ‫אחר‬ ‫פרוטונים‬ ‫מספר‬ ‫של‬ ‫מצב‬ ‫להיות‬ ‫יכול‬
,
81
‫המספר‬ ‫באותו‬ ‫נייטרונים‬ ‫יש‬
.
‫פרוטונים‬ ‫של‬ ‫שונה‬ ‫מספר‬ ‫עם‬ ‫אך‬ ,‫מולרית‬ ‫במסה‬ ‫הזהים‬ ‫אטומים‬ ‫של‬ ‫מצבים‬ ‫גם‬ ‫ייתכנו‬
.
‫ע"י‬ ,‫מכוונת‬ ‫בצורה‬ ‫נוצרו‬ ‫המחזורים‬ .‫מדעי‬ ‫בטחון‬ ‫תחושת‬ ‫ליצירת‬ ‫נועד‬ ‫מחזורי‬ ‫סידור‬
‫רישום‬ ‫יצירת‬
‫להשלמת‬ ‫שנדרש‬ ‫מה‬ ‫פי‬ ‫על‬ ,‫נייטרונים‬ ‫השלמת‬ ‫עם‬ ,‫מסויים‬ ‫פרוטונים‬ ‫מספר‬ ‫על‬
‫דומות‬ ‫תכונות‬ ‫עם‬ ‫חומרים‬ ‫העמודות‬ ‫האותן‬ ‫היו‬ ‫לא‬ ‫אחרת‬ .‫בטבלא‬ ‫המחזורים‬
.
‫שמדענים‬ ‫טענו‬ ‫ודתיים‬ .‫לדת‬ ‫מדע‬ ‫בין‬ ‫קשה‬ ‫מאבק‬ ‫היה‬ ,‫הטבלא‬ ‫כשנוצרה‬ ,‫היסטורית‬
‫יצ‬ .‫להבנה‬ ‫ניתן‬ ‫ולא‬ ‫כאוטי‬ ‫העולם‬ ‫כי‬ ‫לכלום‬ ‫יגיעו‬ ‫לא‬
‫ביתר‬ ‫הגיוני‬ ‫ידע‬ ‫ירת‬
,
,‫בטבלא‬ ‫שיוצר‬ ‫כפי‬
‫נועדה‬
‫כוחות‬ ‫של‬ ‫סודות‬ ‫לגלות‬ ‫מאמצים‬ ‫על‬ ‫לגהנום‬ ‫יגיעו‬ ‫שמדענים‬ ‫שאיימו‬ ‫דת‬ ‫אנשי‬ ‫כנגד‬ ‫כקונטרה‬
‫גדולים‬
.
‫תחמון‬ ‫ע"י‬ ‫שהושגה‬ ,‫יתר‬ ‫הגיוניות‬ ‫של‬ ‫רוח‬ ‫עם‬ ‫עוצבה‬ ‫המחזורית‬ ‫הטבלא‬
.
Periodic table wrong
4/16/2012 7:29:43 PM
The periodic table mentions grams per mole Mole is incorrect due to neglection
of collisions Between particles Smaller particles collide more and thus in give
Volume there were expected less particles than In A mole of larger particles
Grams or weight is wrong since the size causes Gravitation by flow blocking
The larger is the molecule the more flow Is blocked The mass is relevant but
the size is the main parameter Undense and large molecules are heavy Small
and dense molecules are light Immersion or acceleration blocking of A standard
81
string is the precise parameter to Measure mass.
Rust
4/16/2012 7:13:48 PM
Rust is bulks of oxygen stuck to a metal The amount of gas particles to relative
metal in gaseous State particle can be various and not precise The ordinary
equation is not real there are no Distinct molecules but bulk of metal with gas
particles Solids are large connected particle not Sole molecules Gas is sole
particles of various size
Properties of water molecule: hydrogen is light but concentrated
9/11/2011 6:42:20 PM
The properties of water: gravitation of small particles.
Gravitation affects differently on particles with different
sizes. Small particles can be concentrated, but almost
no gravitation effect because low chance to collision with
gravitation particles. The effect is on weight of small
particles which is very low. However, the mass might be high,
and when the particles are connected with particle with large
volume/mass ratio, the effect of small concentrated particles,
is high molecule mass, which blocks effects of random collisions.
There is high speed/mass needed to move such molecules and this
effect leads to the matter to be liquid. Without the oxygen,
which is large in volume/mass, hydrogen is light and
hardly affected by gravitation. Hydrogen, however is concentrated
and with bond to water, causes water molecule to become with large
mass, and thus not easily moved. The effect of gravitation on water
is through oxygen molecule, which is large volume/mass.
82
Hydrogen is small and concentrated: Reactivity
1/5/2012 2:36:09 PM
Hydrogen is reactive. Small and light molecule would not be sufficient for
any molecular damage for other molecules. The hydrogen is heavy and
concentrated, but
small. The small molecule has small surface area for bond with oxygen. The
small bond
is weak bond. The detached hydrogen is heavy and collisions with other
molecules are
serious structure deformators. The heaviness of hydrogen causes only two
hydrogen
particles to make oxygen liquid (seen as water). The gaseous state of hydrogen is
due to smaller size relative to gravitation particles.
water= 6H O1
2/7/2013 4:46:40 PM
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf
ScienceCriticism.pdf

Weitere ähnliche Inhalte

Ähnlich wie ScienceCriticism.pdf

Repeatability and Reproducibility in science
Repeatability and Reproducibility in scienceRepeatability and Reproducibility in science
Repeatability and Reproducibility in science
pramod41kumar
 
A statistical framework for multiparameter analysis at the single cell level
A statistical framework for multiparameter analysis at the single cell levelA statistical framework for multiparameter analysis at the single cell level
A statistical framework for multiparameter analysis at the single cell level
Shashaanka Ashili
 
Session 1 part 2
Session 1 part 2Session 1 part 2
Session 1 part 2
plmiami
 

Ähnlich wie ScienceCriticism.pdf (16)

Lab review
Lab reviewLab review
Lab review
 
Genomics and Proteomics - Impact on Drug Discovery
Genomics and Proteomics - Impact on Drug DiscoveryGenomics and Proteomics - Impact on Drug Discovery
Genomics and Proteomics - Impact on Drug Discovery
 
Umuc biology 102103 Massive Success / snaptutorial.com
Umuc biology 102103 Massive Success / snaptutorial.comUmuc biology 102103 Massive Success / snaptutorial.com
Umuc biology 102103 Massive Success / snaptutorial.com
 
Regents.pdf
Regents.pdfRegents.pdf
Regents.pdf
 
MET EV 2.pptx, metabolimics,genomics,approach
MET EV 2.pptx, metabolimics,genomics,approachMET EV 2.pptx, metabolimics,genomics,approach
MET EV 2.pptx, metabolimics,genomics,approach
 
Open Science and Ecological meta-anlaysis
Open Science and Ecological meta-anlaysisOpen Science and Ecological meta-anlaysis
Open Science and Ecological meta-anlaysis
 
Repeatability and Reproducibility in science
Repeatability and Reproducibility in scienceRepeatability and Reproducibility in science
Repeatability and Reproducibility in science
 
Leonid Asipov vs.pdf
Leonid Asipov vs.pdfLeonid Asipov vs.pdf
Leonid Asipov vs.pdf
 
Leonid Asipov vs.pdf
Leonid Asipov vs.pdfLeonid Asipov vs.pdf
Leonid Asipov vs.pdf
 
GRC_July2016_MAT5
GRC_July2016_MAT5GRC_July2016_MAT5
GRC_July2016_MAT5
 
Writing Introduction for Manuscript (scientific writing)
Writing Introduction for Manuscript (scientific writing)Writing Introduction for Manuscript (scientific writing)
Writing Introduction for Manuscript (scientific writing)
 
A statistical framework for multiparameter analysis at the single cell level
A statistical framework for multiparameter analysis at the single cell levelA statistical framework for multiparameter analysis at the single cell level
A statistical framework for multiparameter analysis at the single cell level
 
Umuc biology 102103 Success Begins / snaptutorial.com
Umuc biology 102103  Success Begins / snaptutorial.comUmuc biology 102103  Success Begins / snaptutorial.com
Umuc biology 102103 Success Begins / snaptutorial.com
 
Session 1 part 2
Session 1 part 2Session 1 part 2
Session 1 part 2
 
Metabolic engineering approaches in medicinal plants
Metabolic engineering approaches in medicinal plantsMetabolic engineering approaches in medicinal plants
Metabolic engineering approaches in medicinal plants
 
Bioinformatica 15-12-2011-t9-t10-bio cheminformatics
Bioinformatica 15-12-2011-t9-t10-bio cheminformaticsBioinformatica 15-12-2011-t9-t10-bio cheminformatics
Bioinformatica 15-12-2011-t9-t10-bio cheminformatics
 

Mehr von Leonid Ledata

мышонок1111111.pdf THE LITTLEMOUSE WILL BE BORN
мышонок1111111.pdf THE LITTLEMOUSE WILL BE BORNмышонок1111111.pdf THE LITTLEMOUSE WILL BE BORN
мышонок1111111.pdf THE LITTLEMOUSE WILL BE BORN
Leonid Ledata
 
мышонок111111.pdf the little mouth mouse
мышонок111111.pdf the little mouth mouseмышонок111111.pdf the little mouth mouse
мышонок111111.pdf the little mouth mouse
Leonid Ledata
 

Mehr von Leonid Ledata (20)

мышонок1111111111.pdf I am a hedgehog mouse and a rabbit
мышонок1111111111.pdf I am a hedgehog mouse and a rabbitмышонок1111111111.pdf I am a hedgehog mouse and a rabbit
мышонок1111111111.pdf I am a hedgehog mouse and a rabbit
 
мышонок11111111.pdf the knig of the truth and not kkkplaz
мышонок11111111.pdf the knig of the truth and not kkkplazмышонок11111111.pdf the knig of the truth and not kkkplaz
мышонок11111111.pdf the knig of the truth and not kkkplaz
 
мышонок1111111.pdf THE LITTLEMOUSE WILL BE BORN
мышонок1111111.pdf THE LITTLEMOUSE WILL BE BORNмышонок1111111.pdf THE LITTLEMOUSE WILL BE BORN
мышонок1111111.pdf THE LITTLEMOUSE WILL BE BORN
 
мышонок111111.pdf the little mouth mouse
мышонок111111.pdf the little mouth mouseмышонок111111.pdf the little mouth mouse
мышонок111111.pdf the little mouth mouse
 
мышонок1111.pdf madeupboy2 the chance of having a boy
мышонок1111.pdf madeupboy2 the chance of having a boyмышонок1111.pdf madeupboy2 the chance of having a boy
мышонок1111.pdf madeupboy2 the chance of having a boy
 
мышонок11.pdf the little mouth mouse the little baby which havo of being born...
мышонок11.pdf the little mouth mouse the little baby which havo of being born...мышонок11.pdf the little mouth mouse the little baby which havo of being born...
мышонок11.pdf the little mouth mouse the little baby which havo of being born...
 
мышонок1.pdf мой второй сын мышонок который должен родится
мышонок1.pdf мой второй сын мышонок который должен родитсямышонок1.pdf мой второй сын мышонок который должен родится
мышонок1.pdf мой второй сын мышонок который должен родится
 
мышонок the second boy of leonid ledata the writer
мышонок the second boy of leonid ledata the writerмышонок the second boy of leonid ledata the writer
мышонок the second boy of leonid ledata the writer
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledataT ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
 
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NTT ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
 
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
 
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradictionT ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
 
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngateT ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
T ВЫСШИЙ ЧИН BIGBADEND the knig of the rankingT ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
T ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
 
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwTGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
 
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAETGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
 
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
 
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
 
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
 
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATHT ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
 

Kürzlich hochgeladen

Goa Call "Girls Service 9316020077 Call "Girls in Goa
Goa Call "Girls  Service   9316020077 Call "Girls in GoaGoa Call "Girls  Service   9316020077 Call "Girls in Goa
Goa Call "Girls Service 9316020077 Call "Girls in Goa
sexy call girls service in goa
 
Desi Bhabhi Call Girls In Goa 💃 730 02 72 001💃desi Bhabhi Escort Goa
Desi Bhabhi Call Girls  In Goa  💃 730 02 72 001💃desi Bhabhi Escort GoaDesi Bhabhi Call Girls  In Goa  💃 730 02 72 001💃desi Bhabhi Escort Goa
Desi Bhabhi Call Girls In Goa 💃 730 02 72 001💃desi Bhabhi Escort Goa
russian goa call girl and escorts service
 
Goa Call Girls 9316020077 Call Girls In Goa By Russian Call Girl in goa
Goa Call Girls 9316020077 Call Girls  In Goa By Russian Call Girl in goaGoa Call Girls 9316020077 Call Girls  In Goa By Russian Call Girl in goa
Goa Call Girls 9316020077 Call Girls In Goa By Russian Call Girl in goa
russian goa call girl and escorts service
 
Call Girls In Goa 9316020077 Goa Call Girl By Indian Call Girls Goa
Call Girls In Goa  9316020077 Goa  Call Girl By Indian Call Girls GoaCall Girls In Goa  9316020077 Goa  Call Girl By Indian Call Girls Goa
Call Girls In Goa 9316020077 Goa Call Girl By Indian Call Girls Goa
sexy call girls service in goa
 
CHEAP Call Girls in Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICE
CHEAP Call Girls in  Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICECHEAP Call Girls in  Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICE
CHEAP Call Girls in Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICE
9953056974 Low Rate Call Girls In Saket, Delhi NCR
 

Kürzlich hochgeladen (20)

Sonagachi ( Call Girls ) Kolkata ✔ 6297143586 ✔ Hot Model With Sexy Bhabi Rea...
Sonagachi ( Call Girls ) Kolkata ✔ 6297143586 ✔ Hot Model With Sexy Bhabi Rea...Sonagachi ( Call Girls ) Kolkata ✔ 6297143586 ✔ Hot Model With Sexy Bhabi Rea...
Sonagachi ( Call Girls ) Kolkata ✔ 6297143586 ✔ Hot Model With Sexy Bhabi Rea...
 
VIP Model Call Girls Koregaon Park ( Pune ) Call ON 8005736733 Starting From ...
VIP Model Call Girls Koregaon Park ( Pune ) Call ON 8005736733 Starting From ...VIP Model Call Girls Koregaon Park ( Pune ) Call ON 8005736733 Starting From ...
VIP Model Call Girls Koregaon Park ( Pune ) Call ON 8005736733 Starting From ...
 
Goa Call "Girls Service 9316020077 Call "Girls in Goa
Goa Call "Girls  Service   9316020077 Call "Girls in GoaGoa Call "Girls  Service   9316020077 Call "Girls in Goa
Goa Call "Girls Service 9316020077 Call "Girls in Goa
 
Hotel And Home Service Available Kolkata Call Girls Howrah ✔ 6297143586 ✔Call...
Hotel And Home Service Available Kolkata Call Girls Howrah ✔ 6297143586 ✔Call...Hotel And Home Service Available Kolkata Call Girls Howrah ✔ 6297143586 ✔Call...
Hotel And Home Service Available Kolkata Call Girls Howrah ✔ 6297143586 ✔Call...
 
Independent Hatiara Escorts ✔ 9332606886✔ Full Night With Room Online Booking...
Independent Hatiara Escorts ✔ 9332606886✔ Full Night With Room Online Booking...Independent Hatiara Escorts ✔ 9332606886✔ Full Night With Room Online Booking...
Independent Hatiara Escorts ✔ 9332606886✔ Full Night With Room Online Booking...
 
↑Top Model (Kolkata) Call Girls Rajpur ⟟ 8250192130 ⟟ High Class Call Girl In...
↑Top Model (Kolkata) Call Girls Rajpur ⟟ 8250192130 ⟟ High Class Call Girl In...↑Top Model (Kolkata) Call Girls Rajpur ⟟ 8250192130 ⟟ High Class Call Girl In...
↑Top Model (Kolkata) Call Girls Rajpur ⟟ 8250192130 ⟟ High Class Call Girl In...
 
𓀤Call On 6297143586 𓀤 Sonagachi Call Girls In All Kolkata 24/7 Provide Call W...
𓀤Call On 6297143586 𓀤 Sonagachi Call Girls In All Kolkata 24/7 Provide Call W...𓀤Call On 6297143586 𓀤 Sonagachi Call Girls In All Kolkata 24/7 Provide Call W...
𓀤Call On 6297143586 𓀤 Sonagachi Call Girls In All Kolkata 24/7 Provide Call W...
 
Desi Bhabhi Call Girls In Goa 💃 730 02 72 001💃desi Bhabhi Escort Goa
Desi Bhabhi Call Girls  In Goa  💃 730 02 72 001💃desi Bhabhi Escort GoaDesi Bhabhi Call Girls  In Goa  💃 730 02 72 001💃desi Bhabhi Escort Goa
Desi Bhabhi Call Girls In Goa 💃 730 02 72 001💃desi Bhabhi Escort Goa
 
Model Call Girls In Velappanchavadi WhatsApp Booking 7427069034 call girl ser...
Model Call Girls In Velappanchavadi WhatsApp Booking 7427069034 call girl ser...Model Call Girls In Velappanchavadi WhatsApp Booking 7427069034 call girl ser...
Model Call Girls In Velappanchavadi WhatsApp Booking 7427069034 call girl ser...
 
Almora call girls 📞 8617697112 At Low Cost Cash Payment Booking
Almora call girls 📞 8617697112 At Low Cost Cash Payment BookingAlmora call girls 📞 8617697112 At Low Cost Cash Payment Booking
Almora call girls 📞 8617697112 At Low Cost Cash Payment Booking
 
Hotel And Home Service Available Kolkata Call Girls South End Park ✔ 62971435...
Hotel And Home Service Available Kolkata Call Girls South End Park ✔ 62971435...Hotel And Home Service Available Kolkata Call Girls South End Park ✔ 62971435...
Hotel And Home Service Available Kolkata Call Girls South End Park ✔ 62971435...
 
Dakshineswar Call Girls ✔ 8005736733 ✔ Hot Model With Sexy Bhabi Ready For Se...
Dakshineswar Call Girls ✔ 8005736733 ✔ Hot Model With Sexy Bhabi Ready For Se...Dakshineswar Call Girls ✔ 8005736733 ✔ Hot Model With Sexy Bhabi Ready For Se...
Dakshineswar Call Girls ✔ 8005736733 ✔ Hot Model With Sexy Bhabi Ready For Se...
 
↑Top Model (Kolkata) Call Girls Howrah ⟟ 8250192130 ⟟ High Class Call Girl In...
↑Top Model (Kolkata) Call Girls Howrah ⟟ 8250192130 ⟟ High Class Call Girl In...↑Top Model (Kolkata) Call Girls Howrah ⟟ 8250192130 ⟟ High Class Call Girl In...
↑Top Model (Kolkata) Call Girls Howrah ⟟ 8250192130 ⟟ High Class Call Girl In...
 
Goa Call Girls 9316020077 Call Girls In Goa By Russian Call Girl in goa
Goa Call Girls 9316020077 Call Girls  In Goa By Russian Call Girl in goaGoa Call Girls 9316020077 Call Girls  In Goa By Russian Call Girl in goa
Goa Call Girls 9316020077 Call Girls In Goa By Russian Call Girl in goa
 
📞 Contact Number 8617697112 VIP Ganderbal Call Girls
📞 Contact Number 8617697112 VIP Ganderbal Call Girls📞 Contact Number 8617697112 VIP Ganderbal Call Girls
📞 Contact Number 8617697112 VIP Ganderbal Call Girls
 
Call Girls In Goa 9316020077 Goa Call Girl By Indian Call Girls Goa
Call Girls In Goa  9316020077 Goa  Call Girl By Indian Call Girls GoaCall Girls In Goa  9316020077 Goa  Call Girl By Indian Call Girls Goa
Call Girls In Goa 9316020077 Goa Call Girl By Indian Call Girls Goa
 
CHEAP Call Girls in Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICE
CHEAP Call Girls in  Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICECHEAP Call Girls in  Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICE
CHEAP Call Girls in Malviya Nagar, (-DELHI )🔝 9953056974🔝(=)/CALL GIRLS SERVICE
 
Call Girls Manjri Call Me 7737669865 Budget Friendly No Advance Booking
Call Girls Manjri Call Me 7737669865 Budget Friendly No Advance BookingCall Girls Manjri Call Me 7737669865 Budget Friendly No Advance Booking
Call Girls Manjri Call Me 7737669865 Budget Friendly No Advance Booking
 
College Call Girls Pune 8617697112 Short 1500 Night 6000 Best call girls Service
College Call Girls Pune 8617697112 Short 1500 Night 6000 Best call girls ServiceCollege Call Girls Pune 8617697112 Short 1500 Night 6000 Best call girls Service
College Call Girls Pune 8617697112 Short 1500 Night 6000 Best call girls Service
 
VIP Model Call Girls Budhwar Peth ( Pune ) Call ON 8005736733 Starting From 5...
VIP Model Call Girls Budhwar Peth ( Pune ) Call ON 8005736733 Starting From 5...VIP Model Call Girls Budhwar Peth ( Pune ) Call ON 8005736733 Starting From 5...
VIP Model Call Girls Budhwar Peth ( Pune ) Call ON 8005736733 Starting From 5...
 

ScienceCriticism.pdf

  • 1. 1
  • 2. 2 L-Data Science Criticism Leonid Asipov ‫לעתיד‬ Ph.D Introduction 4-5 ‫מדעית‬ ‫ואמינות‬ ‫מדעיות‬ ‫תוצאות‬ ‫הצגת‬ Botany 5-59 Botany research is not needed anymore L-Data vs "Expression of cyanobacterial ictB in higher plants enhanced photosynthesis and growth" L-Data vs "Enhanced photosynthesis and growth of transgenic plants that express ictB, a gene involved in HCO 3–accumulation in cyanobacteria" L-Data vs "A putative HCO transporter in the cyanobacterium Synechococcus sp. strain PCC 7942 " The Ci parameter is WRONG L-Data vs "CO2 Concentrating Mechanisms in Algae: Mechanisms, Environmental" L-Data vs: Development of synchronized, autonomous, and self-regulated oscillations in plant L-Data vs “The Role of Tobacco Aquaporin1 in Improving Water Use Efficiency, Hydraulic Conductivity, and Yield Production Under Salt Stress1” The reason for the lack of photosynthesis after dehydration is lack of water for enzymes
  • 3. 3 Changes in the properties of reaction center II during the initial stages of photo inhibition as revealed by thermo luminescence measurements SYNERGISM OF LIGHT AND FUSICOCCIN ON STOMATAL OPENING" CO2 not light is the trigger for leaf conductance to gases WUE CANNOT GROW There is no main growth control in: transpiration is related to nutrient transport Cactuses create sugar during the day There is no CO2: O2 concentration blocks photosynthesis by reverse CO2 formation Stomata don't affect photosynthesis CO2 Channels Have to Enlarge Transpiration Human industry is nature's best friend No need for CO2 channel for chloroplast L-Data vs Rapid assessing of water and nitrogen status in wheat flag leaves Physics 59-78 Light Speed is not Constant Relativity Theory Wrong Matter does not have inner structure There is no minimum speed needed to fly to space Gravitational force is related to Surface Area/Mass ratio
  • 4. 4 The particles are not of right form. Sand, salt and any other materials There is no possibility of creating ideal space Classical energy equation is wrong : energy is speed/mass Movement of masses is enough to explain all physical or chemical phenomena The nature of forces : Gravitational force Thermodynamics are false ‫החשמל‬ ‫חליקי‬ ‫ע"י‬ ‫חום‬ ‫ופיזור‬ ‫גדולים‬ ‫מוליכים‬ ‫של‬ ‫אלסטיות‬ : ‫חשמל‬ Light Speed Changes with collisions ZEREM LO NAHON RADIO WAVE DIFFERS SPEED NOT FREQUENCY Light Speed is not uniform, WAVES WRONG Electricity ERROR: Science fraud PIGMENT REFLECTION : ABSOBTION OF SPEED Chemistry 78-82 Periodic Table might be a falsie Periodic table wrong Rust Properties of water molecule : hydrogen is light but concentrated Hydrogen is small and concentrated : Reactivity
  • 5. 5 water= 6H O1 Biology 82-90 Model of enzyme of a connector can explain DNA replication as a spontaneous diffusion. Enzymes does not need any energy for function Protein construction control by Proteins No X inactivation exist GENETICS ARE WRONG ‫אפשריים‬ ‫לא‬ ‫כרומזומליים‬ ‫שחלופים‬ Differentiation of cells : protein - protein replication DNA IS SINGLE STRANDED Science 90-95 Science Courthouse : ‫מדע‬ ‫ופעילות‬ ‫מימון‬ ‫צורות‬ Models are proof of understanding : A new science concept ‫קיים‬ ‫לא‬ ‫מימד‬ ‫זמן‬ ‫לבד‬ ‫לפרסם‬ ‫צריכים‬ : ‫צודק‬ ‫לא‬ ‫בארץ‬ ‫המדע‬ ‫מדע‬ ‫של‬ ‫סטודנטים‬ ‫למכור‬ ‫אסור‬ ‫מדע‬ ‫מדע‬ ‫של‬ ‫חירטוטים‬ ‫במכון‬ ‫אסרים‬ ‫ניסויים‬
  • 6. 6 University Unfair ‫אישורים‬ 96 Introduction Scientific Honesty and Accuracy 1/5/2011 11:53:12 AM ‫מדע‬ ‫תוצאות‬ ‫הצגת‬ ‫מדעית‬ ‫ואמינות‬ ‫יות‬ ‫וסטודנטים‬ ‫מדע‬ ‫אנשי‬ ‫בין‬ ‫להטמעה‬ ‫מאד‬ ‫חשוב‬ ‫מושג‬ ‫היא‬ ‫מדעית‬ ‫אמינות‬ ‫נתונים‬ ‫עיבוד‬ ,‫מדידה‬ ‫באופן‬ : ‫אמינות‬ ‫לחוסר‬ ‫מקומות‬ ‫הרבה‬ ‫טומנים‬ ‫מדעיים‬ ‫ניסויים‬ ,‫למעשה‬ , ‫אמינות‬ ‫וחוסר‬ ‫לנבוע‬ ‫יכולות‬ ‫מדעיים‬ ‫ממחקרים‬ ‫מוטעות‬ ‫מסקנות‬ .‫המסקנות‬ ‫והסקת‬ ‫הנתונים‬ ‫הצגת‬ ‫ומהווים‬ ‫אמינות‬ ‫מחוסר‬ .‫המחקר‬ ‫על‬ ‫שהתבססו‬ ‫אחרים‬ ‫וחוקרים‬ ‫עצמו‬ ‫לחוקר‬ ‫משאבים‬ ‫של‬ ‫בזבוז‬ ‫מדעי‬ ‫סתום‬ ‫במבוי‬ ‫נמצאים‬ ‫חוקרים‬ ‫הרבה‬ ‫כזאת‬ . ‫אמינות‬ ‫רבדי‬ ‫מספר‬ ‫יש‬ ‫מדעיים‬ ‫ניסויים‬ ‫של‬ ‫לתוצאות‬ : 1 . ‫עצמו‬ ‫הניסוי‬ ‫ביצוע‬ ‫ברמת‬ ‫אמינות‬ ‫אפשריים‬ ‫לארטיפקטים‬ ‫לב‬ ‫תשומת‬ ‫המדידות‬ ‫ביצוע‬ ‫נכונות‬ 2 . ‫ב‬ ‫אמינות‬ ‫הניסוי‬ ‫תוצאות‬ ‫עיבוד‬ ‫רמת‬ ‫התוצאות‬ ‫בעיבוד‬ ‫טעויות‬ ‫המידע‬ ‫כל‬ ‫לעומת‬ ‫הקיים‬ ‫מהמידע‬ ‫חלקים‬ ‫הצגת‬ ‫כמותית‬ ‫מול‬ ‫איכותית‬ ‫הצגה‬ ,‫בסטטיסטיקה‬ ‫שימוש‬ ‫חוסר‬ 3 . ‫התוצאות‬ ‫הצגת‬ ‫ברמת‬ ‫אמינות‬ ‫מידע‬ ‫החסרת‬ 4 . ‫שיש‬ ‫הראיות‬ ‫לכל‬ ‫התייחסות‬ : ‫מסקנות‬ ‫הסקת‬ ‫ברמת‬ ‫אמינות‬ ‫ש‬ ‫אפשרות‬ ‫שתומן‬ ‫נוסף‬ ‫עניין‬ ( ‫במחקר‬ ‫אחריות‬ ‫חלוקת‬ ‫הוא‬ ‫אמינות‬ ‫חוסר‬ ‫ל‬ ,‫נתונים‬ ‫עיבוד‬ ,‫מדידה‬ ‫מסקנו‬ ‫הסקת‬ )‫ת‬ ‫לאיתור‬ ‫קשה‬ ‫הוא‬ ‫שלב‬ ‫בכל‬ ‫אמינות‬ ‫חוסר‬ .‫מספקת‬ ‫מרכזית‬ ‫בקרה‬ ‫והעדר‬ . ‫אמינות‬ ‫וחוסר‬ ‫לטיוח‬ ‫פעמים‬ ‫הרבה‬ ‫מובילה‬ ‫במחקר‬ ‫המשתתפים‬ ‫האנשים‬ ‫כל‬ ‫בין‬ ‫אובייקטיביות‬ ‫העדר‬ ‫של‬ ‫בתוצאות‬ ‫שמעוניינים‬ ‫השונים‬ ‫הגורמים‬ ‫אחרות‬ ‫ולא‬ ‫מסויימות‬ .
  • 7. 7 Botany Botany research is not needed anymore 8/21/2011 12:38:48 PM ‫הידע‬ ‫כל‬ ‫את‬ ‫כבר‬ ‫יש‬ : ‫בוטניקה‬ ‫יותר‬ ‫צריך‬ ‫לא‬ . ‫יותר‬ ‫נחוצים‬ ‫שלא‬ ‫נושאים‬ ‫צורך‬ ‫ללא‬ ,‫סתם‬ ‫חוקרים‬ ‫המדענים‬ . ‫המדענים‬ .‫נוסף‬ ‫למחקר‬ ‫צורך‬ ‫אין‬ ,‫מספיק‬ ‫ידוע‬ ,‫הצמח‬ ‫מדעי‬ ‫של‬ ‫בנושאים‬ ‫הביתה‬ ‫לחזור‬ ‫יכולם‬ ‫התעסוקה‬ ‫מלשכת‬ ‫קצבה‬ ‫ולקבל‬ . ‫שאנושות‬ ‫שנחוץ‬ ‫מה‬ ‫כל‬ - ,‫גודל‬ : ‫רצויות‬ ‫תכונות‬ ‫עם‬ ‫זנים‬ ‫מטפח‬ ‫הזעים‬ ‫בנק‬ .‫צמחים‬ ‫של‬ ‫זרעים‬ ‫בנק‬ ‫מלח‬ ‫ריכוז‬ ,‫סוכר‬ ‫ריכוז‬ ,‫פירות‬ ‫של‬ ‫מקסימלי‬ ‫מספר‬ , ‫הטיפוח‬ .‫גדילה‬ ‫מהירות‬ ,‫חלבון‬ ‫ריכוז‬ - .‫פשוט‬ ‫הצמחי‬ ‫את‬ .‫צמחים‬ ‫מגדלים‬ ‫קדם‬ ‫בימי‬ ‫כמו‬ ‫הטיפוח‬ .‫המשך‬ ‫בדור‬ ‫מרבים‬ ‫טובים‬ ‫הכי‬ ‫ם‬ - ‫דורות‬ ‫מספר‬ , ‫האחרון‬ ‫הדור‬ - ‫פעם‬ ‫זרעיים‬ ‫קונים‬ ‫חקלאים‬ .‫זנים‬ ‫והרבה‬ ‫מינים‬ ‫הרבה‬ ‫יש‬ ‫הזרעיים‬ ‫בבנק‬ .‫לזרעיים‬ ‫אחת‬ - ‫לזרעיים‬ ‫שגודל‬ ‫צמחים‬ ‫של‬ ‫מסויים‬ ‫אחוז‬ ‫יבול‬ ‫בכל‬ ‫לחקלאי‬ ‫יש‬ ‫כך‬ ‫אחר‬ . ‫ד‬ ‫נג‬ .‫וטבק‬ ‫שום‬ : ‫טבעיים‬ ‫אמצעים‬ ‫יש‬ ‫מזיקים‬ ‫חרקים‬ ‫נגד‬ ‫עבודת‬ ‫של‬ ‫אמצעים‬ ‫יש‬ ‫שוטים‬ ‫עשבים‬ ‫השוטים‬ ‫והצמחים‬ ‫בשדה‬ ‫בתחרות‬ ‫מנצחים‬ ‫שמגדלים‬ ‫הצמחים‬ ‫רוב‬ .‫אדם‬ ‫מופיעים‬ ‫לא‬ . ‫שנים‬ ‫במספר‬ ‫פעם‬ ‫בשדה‬ ‫אחרות‬ ‫וקטניות‬ ‫שועית‬ ,‫אפונה‬ ‫גידול‬ ‫עם‬ ‫אפשריים‬ ‫טבעיים‬ ‫דשנים‬ . ‫השקייה‬ - ‫השוטים‬ ‫העשבים‬ ‫כמות‬ ‫את‬ ‫שמקטינה‬ ‫טפטוף‬ ‫עם‬ ‫רגילה‬ - ‫ה‬ ‫לייד‬ ‫ההשקייה‬ ‫כי‬ ‫צמחים‬ ‫לעשבים‬ ‫אור‬ ‫שממסכים‬ . ‫אקמית‬ ‫השכלה‬ ‫דורשים‬ ‫לא‬ ‫ייחורים‬ ‫גידול‬ ‫כגון‬ ‫פשוטים‬ ‫בוטניים‬ ‫אמצעים‬ . ‫הצמחים‬ ‫תורת‬ ? ‫כסף‬ ‫קובלתם‬ ‫מה‬ ‫על‬ ?‫תרומתכם‬ ‫מה‬ ‫בוטנאים‬ - .‫כלום‬ ‫הוסיפה‬ ‫לא‬ ‫המודרנה‬ .‫עתיקה‬ L-Data vs "Expression of cyanobacterial ictB in higher plants enhanced photosynthesis and growth" Leonid Asipov 11/15/2012 10:57:26 AM From letter to the editorial director of the magazine… "My Name is Leonid Asipov. I am the co-author of the article and the worker which accomplished the experiments. The data results are insignificant and no conclusions are possible on basis of the results. Whole experiments were neglected by the main author of the article and the conclusions are scientific lies… "
  • 8. 8 TOPICS 1. ONLY PARTIAL DATA WAS PRESENTED. THE ADDITIONAL PLANTS SHOW NO SIGNIFICANCE RELATIVE TO WT. 2. SECOND GENERATION OF PLANTS, GROWN OUT OF THE BEST 1-ST GENERATION PLANTS, SHOW NO SIGNIFICANCE AT ALL. 3. FALSE CONCLUSION IN ABSTRACT OF THE ARTICLE, ACCORING TO THE AUTHOR, THE ENHANCEMENT OF PHOTOSYNTHESIS IN ONLY AT LIMITING CO2 CONDITIONS, HOWEVER, THE RESULTS (BASED ON ONLY 4 PLANTS) SHOW THAT AT ALL CO2 LEVELS, THE TRANSGENIC PLANTS ARE BETTER. NOTE THAT MOST OF THE DATA (THE REST 33 TRANSGENIC PLANTS) WAS NOT PUBLISHED. 4. No significance tests were calculated. The group of transgenic plants is too small(4 plants). THE REAL DATA: EMPIRIC MEASUREMENTS OF GAS-EXCHANGE OF LEAVES: WT VS TRANSGENIC PLANTS. Time of day during measurement The measurement is Gas-Exchange of single leaves. Transgenic and wt plants are similarly measured by the gas-exchange device, in elevating CO2 concentrations and in similar temperature and light conditions. The time of day of the measurement of each plant was different. Part of the plants were measured during morning and part, during the evening. The difference of the Photosynthesis rate between the different times of day is great. During the evening, the plants were photosynthesizing may hours already and part of the enzymes needs regeneration. Moring is hours of highest photosynthesis rates. Note that there is large differences between the photosynthesis and transpiration rates of the plants. Part of the large differences is due to different time of day during measurement.
  • 9. 9 Scientific conclusions The plants are to be measured in equal conditions: equal time of day and equal age. When the experiment is large, the task is impossible. Since the gas-exchange measurement device is based on a single leaf and is expensive, in addition to long measurement time of the CO2 curve (at least 15 minutes for each plants + acclimation time) and large number of plants demanding other method for plant screening for better photosynthesis and transpiration properties. Measurement in a single CO2 concentration is partial solution for possible measurement of many plants in short period of time. The results of the article: partial data was presented. The transgenic plants are to be considered similar "treatment" and a large number of plants are to be measured and T significance tests are to be calculated. If some particular transgenic plant is for any reason considered to be "other treatment", the plant is to be cloned to next generation and large amount of plants are to be measured in order to perform T significance tests. Second generation of arabidopsis(NOT PUBLISHED DATA) Second generation was cloned of the 4 considered transgenic plants and the results showed no significant difference relative to wt plants. THE PAPER WAS PUBLISHED WITH WRONG CONCLUSIONS BASED ON PARTIAL DATA, WHEN ALL DATA WAS ABUNDANT. THE SCIENTISTS ARE LIERS. Presentation of all the data The real Gas-Exchange data. The first experiment included 37 transgenic arabidopsis plants vs 7 wt plants. The published chart however consists of only 4 transgenic plants. The researchers have neglected all the rest 33 plants and CONCLUDED FALSE conclusions, according to which the transgenic protein has positive effect on photosynthesis of plants. The absence of statistics The variation of the plants is high and T significance tests of all the 37 plants vs
  • 10. 11 wt show no significance at all. The experiment of protein transformation is to be considered similar “treatment”. The 4 top plants were cloned to the next generation and similar tests were conducted. No significance was seen. Original chart published in paper: only 4 plants of 37 transgenic plants were published. Note the inconsistent conclusion in abstract of the article : “photosynthesis was enhanced under limiting and not under saturating CO2 concentrations…” ALL 4 transgenic plants are better photosynthetically at ALL CO2 points(NOTE THE REST 33 PLANTS WHICH ARE NOT). All 37 transgenic plants. Only top 4 were published. The rest of the plants are with large variance. Better or worse than wt. Wt is also variant. Note that only 6 wt plants were measured. More plants, more variance.
  • 11. 11 THE PUBLISHER DID PUBLISH ONLY 4 TRANSGENIC PLANTS. REST OF THE DATA WAS HIDDEN. THE SCEITIFIC FRAUD WAS CAUGHT!!!!!!!!!!!!!! 2nd generation arabidopsis (number of plants > 30). UNSIGNIFICANT DIFFERENCES BETWEEN WT AND THE TRANSGENIC. The protein was also tested in other plants. Similar gas-exchange experiments were conducted.
  • 12. 12 The protein does NOT enhance photosynthesis or prevent transpiration… Soybean experiment. The theory behind the article is wrong. Theoretic criticism. The CO2 channel in chloroplast is not needed : the membrane keeps the unique environment in chloroplast. Channels conduct to and from the chloroplast. The reason of the membrane is to block particles from diffusion from the chloroplast to the cytoplasm. Additional CO2 channel in chloroplast membrane would have forced diffusion of small and medium particles from the chloroplast to the cytoplasm, slowing the enzymatic processes in the chloroplast. The diffusion of CO2 to chloroplast is fast due to large surface area/volume ratio. The main slowing process is diffusion through the cytoplasm. The surface area of a chloroplast is large relative to volume…. The main obstruction to diffusion of CO2 from the air the chloroplast is cytoplasm volume and cytoplasm membrane… The distance of diffusion is the whole cytoplasm… The distance of diffusion through the chloroplast is small and don't forget the large surface area / volume ratio of the chloroplast which cause fast diffusion through the membrane. The conclusion is that no CO2 channels are needed to
  • 13. 13 chloroplast membrane. Addition of CO2 channel, don't enhance any processes in the plant cell…And might slow some processes by diffusion of needed particles from chloroplast to the cell cytoplasm. Bacterial proteins does not express properly in higher organisms. Note folding proteins which are needed. Expression of bacterial protein in higher plants: wrong concept. It is well known in science that proteins from eukaryotes cannot be easily expressed in prokaryotes. In order to create the antibodies, mammal or fly biological systems are needed. The 3rd structure of the proteins need folding proteins. Spontaneous expression of a bacterial protein in plants is not likely to produce a proper protein. The folding proteins of the plant are not compatible. A whole set of additional proteins are needed in order to create a proper protein. The ribosomes of bacteria are different, suggesting the building of the proteins is as well. Possible even the genetic code differs and codons code to other amino acids. The task of the researchers is to prove the protein function, 3-d structure and proper attachment of the protein in the chloroplast membrane. Since the protein was described as "putative", and no 3d structure was proved, as well as no positioning in the chloroplast membrane, it is impossible to claim any biological effect. The foreign bacterial protein may be malfunctional and not even expressed in chloroplast. The difference of the plants in sampling time The experiment was sampled during 2 months and life of arabidopsis plant is about 3 months only. The first plants were the best transgenics, the control plants were measured during the last 4 days of experiment. The control plants were 2 months older than the best (published) transgenics. The difference of the plants is mostly at transpiration parameter, not photosynthesis, and the root system of the transgenic plants which are older by two months is obviously larger, and thus the transpiration. The difference between the transgenic and wt plants is due to age, no genetic differences. External CO2 vs Photosynthesis (wt vs best transgenics)
  • 14. 14 External CO2 vs transpiration: transpiration of wt plants is larger (most possibly because of root system) The ages and sampling dates :
  • 15. 15 PROPER SCIENTIFIC WAY The proper way of molecular biology is protein crystallization to prove proper function : CO2 channel. CO2 channels, as any channel is a pipe-like protein. The pipe is only molecule size specific. CO2 channels conduct medium and small size molecules such as water, CO2, H and other. Proof of protein expression and positioning in chloroplast membranes. The protein has to be proven to have proper channel function and positioning. ONLY AFTER PROPER MOLECULAR PROOF, transgenic plants are to be grown and compared with wt plants. BEFOREHAND we cannot even ask any scientific question, since the protein may be not a channel, not properly built by the plant protein building machinery or not to be positioned in the chloroplast membrane. GUESSING is not proper science. THEORY HAS TO PERCEDE the scientific research with all the possibilities of the results.
  • 16. 16 The Ci parameter : wrong science The Ci parameter is wrong. Photosynthesis is linked to X and Y axis on Ci-Photo charts. Proper comparison is possible between curves is possible at similar X points. The empirically changed parameter is the external CO2 concentration (CO2R). Photosynthesis and Transpiration are biological parameters affected by the external CO2 change. The proper way to compare the effect of external CO2 on photosynthesis and transpiration is to present the external [CO2] on the X axis and plot 2 separate curves for transpiration and photosynthesis. The Ci parameter is wrong : Ci = External [CO2] * STOMATA APERTURE AS TRANSPIRATION * pressure / PHOTOSYNTHESIS * leaf temperature One Ci, different Photosynthesis and transpiration values. If transpiration and photosynthesis is high, the ci is same if transpiration and photosynthesis is low. When plotting relative to photosynthesis, points are compared at different X values, which are related to Y (PHOTOSYNTHESIS) . The ci parameter cannot tell anything about the real properties. Since it is basically ratio of TRANSPIRATION/PHOTOSYNTHESIS. PHOTOSYNTHEIS and TRANSPIRATION IS TO BE PRESENTED AS FUNCTION OF EXTERNAL CO2 CONCENTRATION. L-Data vs "Enhanced photosynthesis and growth of transgenic plants that express ictB, a gene involved in HCO 3–accumulation in cyanobacteria"
  • 17. 17 11/15/2012 11:46:40 AM Criticism Under low relative humidity, the stomata are more open, and there is more transpiration. What is the limiting photosynthesis factor under low humidity relative to high humidity? The ONLY factor which is to consider is transpiration. Under lower humidity concentrations, there is higher concentration of CO2 in the air, and thus photosynthesis is ENHANCED. The protein works more at lower air humidity, ONLY AT HIGHER CO2 concentrations. The protein should be a difference at limiting CO2 conditions, not low humidity. Internal article inconsistency. Since there almost no PHOTOSYNTHESIS dependence on stomata aperture(Fig 1), The photosynthesis under any humidity is relatively constant. The only factor is humidity which lowers the CO2 concentration of the air. According to the article, at lower CO2 concentrations, WT plants are better. WT is the better CO2 conductor than the transgenic plants. The "faster" growth of the transgenic plants at lower humidity, most possibly is accompanied with higher transpiration rates. Why the Water USE Efficiency parameter was not calculated? The transpiration was reported to be similar between the wt and the transgenic plants. Why more growth at lower humidity, at higher CO2? "CO2 compensation point": the plant is breathing more CO2. More energetically demanding plant.
  • 18. 18 At lower CO2 concentration, there is almost no photosynthesis. The plant is breathing and CO2 is diffusing from the plant to the air. If the plant is more sugar demanding there is more breathing and thus, there will be more CO2 absorption when the external CO2 is lower. The expression of additional protein in energetically demanding. The plants breathe more, thus lower compensation point, due to higher CO2 in the leaf. The CO2 compensation point is related to the two first measurements of Photosynthesis and Transpiration. The usual CO2 concentration is 50 and 100 ppm. The compensation point is a calculated point between 50 and 100 ppm at which photosynthesis becomes more than 0. If plant breathes more, at similar photosynthesis, There is more CO2 after the air travels through the chamber, and the result is LESS photosynthesis. Due to breathing, there could be two reasons for lower compensation point. 1. More photosynthesis 2. Bore breathing, which leads to CO2 diffusion from the leaf to the air out of the plant. The advice is to measure photosynthesis at higher concentrations, when to the additional from plant breathing CO2 there is less meaning. The real DATA The number of plants is only 4 transgenic plants vs 1 wt. How is it possible to conclude anything of so few plants? Part of the transgenic plants are not better, how to explain? If part of transgenic plants are to consider other properties than the rest of the plants, the plants should have been cloned to the next generation and large number of plants were supposed to be sampled. Conclusions are to be made on basis of T significance tests of many plants and not on only 4 transgenic and 1 wt plants. The Arabidopsis conclusion was based on 2 transgenic and 1 wt plant. Conclusions could have been made due to too small plants group. The wt plants are relatively at the average of the transgenic plants CO2 curve. Why the conclusion was that the transgenic plants are better, when some transgenic plants was not?
  • 19. 19 Why DO we care about photosynthesis at limiting conditions. The real conditions at fields are not limiting. There is no advantage of transgenic plants and NO transpiration difference. The stomata respond to CO2 with closure. Better photosynthesis, lower CO2, more stomata aperture and MORE TRANSPIRATION. If the plants are better at Lower humidity, MORE TRANSPIRATION is EXPECTED but not reported. CO2 is the main trigger of stomata aperture, and photosynthesis is related to CO2 concentration in the leaf and near the stomata. At high photosynthesis, more transpiration is expected. DATA INSTABILITY: There was too many samples and the AIR CO2 concentration at every sample is not clear. Either the researchers were only slightly changing the AIR CO2 concentration, or there was no enough time of acclimation to the external CO2 conditions. The differences in Ci is at almost every sample, was there a real change is the air CO2? MORE ACCLIMATION TIME IS TO BE GIVEN TO THE PLANT AFTER CO2 changes in the air. The Ci parameter has to be constant and only then the sample to be taken. There are more than 20 samples of every plant and the concentration of CO2 was changed between 50 and ~800 (UNMENTIONED AT ARTICLE) . Was the external CO2 changed at every point? Why there is more than one sampling at a single CO2 point? Acclimation times and CO2 concentration points were unmentioned at Materials and Methods. Growth results There was too few plants to conclude any conclusion. Conclusions are to be based on large number of plants and statistical T tests are to be performed. The growth in lower humidity (which is seen as higher photosynthesis measured at the external to leaf air) would have affected transpiration. Lower CO2 of the air is causing stomata aperture to grow, and thus the transpiration rate is HIGHER. The transgenic plants are more water DEMANDING, which is not positive to
  • 20. 21 agriculture. "The experiments were held 6 times", where are the results? Why the statistical data was not presented? 70% RH is wide and common humidity. Under many agricultural applications there is no advantage of the protein. WHY TO ADVANCE PARTIAL AND UNCLEAR SOLUTION which is not better at most cases? Conclusions 1. The better photosynthesis at lower humidity is not logical. There is more CO2 in the air. Better CO2 assimilation is of WT plants. The protein enhances CO2 transport only at higher CO2 concentrations. INCONSISTENCY. The transgenic plants do not grow better at lower co2 concentrations, but at higher ones. 2. The limiting CO2 concentrations are not real at earth. Normal air has no limitation of CO2 (~400ppm). No advantage of the protein. 3. NO EFFECT ON TRANSPIRATION WAS REPORTED, which is impossible. More photosynthesis, more stomata aperture, since stomata are triggered by CO2 concentration. 4. Higher transpiration rates are expected from transgenic plants due to higher photosynthesis. The transgenic plants are WATER DEMANDING. 1. The water use efficiency was NOT CALCULATED. 2. Experiments were based on SMALL NUMBER OF PLANTS. 3. NO STATISTICS WERE PRESENTED. CONCLUSIONS ARE IMPOSSIBLE BASED ON ONLY 4 TRANSGENIC AND 1 WT PLANT. TRANSGENIC PLANTS AS PRESENTED ARE MORE WATER DEMANDING AND GROW BETTER AT HIGHER CO2 CONCENTRATIONS (LOW HUMIDITY) The CO2 is the main stomata trigger. More photosynthesis, more transpiration.
  • 21. 21 Fig 1 Decoupling of photosynthesis and transpiration, after alterations of CO2 concentration, proves that there is no significant dependence of stomata and photosynthesis. The CO2 diffusion is fast and stomata limit mostly evaporation, while the photosynthesis is almost not affected by stomata change of conductance. L-Data vs "A putative HCO transporter in the cyanobacterium Synechococcus sp. strain PCC 7942 " 11/15/2012 11:54:34 AM CO2 CHANNELS ARE NOT NEEDED IN BACTERIA Bacteria have large surface area/volume ration, and thus there is no channels needed to conduct water or CO2. The mutant, which need more CO2 for proper photosynthesis rate, can be defected in any photosynthetic-related protein, not especially CO2 channels. If there are CO2 channels in bacteria, why to add a channel to a chloroplast?
  • 22. 22 The channels in bacteria would have forced small molecules such as H, H2O, CO2 And many more molecules to diffuse out of the bacterium. To cytoplasm wants to Remain UNIQUE and thus any channel force larger particle concentrations. EUKARYOTIC cells are large, with SMALLER surface area/ volume ratio, and thus Channels are necessary. A CHLOROPLAST IS A CYANOBACTERIA. CHANNELS ARE CONSISTENT. THE TERM "CCM" "CCM" CO2 concentrating mechanisms. It is impossible to concentrate CO2, ONLY to transport faster to the cell. The diffusion rate is related to Concentration of CO2 and to the speed of conduction of CO2 by the cell wall. There is no artificial concentrating mechanisms, only diffusion. THE PROTEIN MIGHT BE NOT A CHANNEL Since the "putative" protein is not necessarily a CO2 channel, which are not expected to be expressed in bacteria, expression in plant will not initiate any change. First the structure of the protein to BE crytallized and proved AS a CHANNEL, pipeous proteins are channels. THEN, the protein has to be proved to be positioned in the chloroplast membrane. ERROR IN FIGURE : "External Ci" concentration. External = CO2R. There is NO Ci in cyanobacteria. Ci is concentration of CO2 in the leaf.
  • 23. 23 The transporter does not influence the concentration, only rate of acclimation to the CO2 change surrounding the cell. The external concentration of CO2 is large (mmol) and the photosynthesis is slow (micromoles) and therefore, after change in external CO2, ACCLIMATION TO ALTERATIONS IN CO2 There is acclimation during which the inner cell concentration becomes as the external concentration of CO2. The uptake is slow, and during uptake, the cell concentration barely changes. The rate of diffusion of co2 is LARGE, therefore, the cell concentration is CONSTANT and similar to OUTER (IN CASE OF EQUAL OTHER SOLUTE CONCENTRATION). If the acclimation time after change in external CO2 is long, there should be no difference between the mutants and the wt. Since in the mutant there is already full steady state photosynthesis (starting at 15mM HCO3 or 6 mM CO2), the rate of diffusion is BEYOND the scope of the limitation of photosynthesis. The real limiting factor of the mutant relative to wt is SOME PROTEIN NEEDED FOR PHOTOSYNTHETIC REACTIONS. The diffusion of CO2 is already at saturation, and all the cytoplasm is with concentration of CO2 similar to the external. Since, the mutants need more CO2 and not only more acclimation, the mutants are impaired in photosynthesis Proteins expression. LESS PROTEINS, LESS PHOTOSYNTHESIS. HIGHER CO2 FOR SILIMAR PHOTOSYNTHESIS. TIME BETWEEN THE MEASUREMENTS(CHART 2) WHAT WAS THE TIME BETWEEN MEASUREMENTS(AFTER ALTERATIONS IN EXTERNAL CO2)? In CASE of the protein as a CO2 channel, more acclimation, would result in
  • 24. 24 similar PHOTOSYNTHESIS at similar [CO2]. At shorter times, there is still uneven [CO2] between The surroundings and the cytoplasm. More CO2 needed for faster diffusion and similar Photosynthesis. In case, time does not force any similarity of PHOTOSYNTHESIS of wt and the treatment, THE PROTEIN IS NOT A CHANNEL . "The protein encoded by ORF467 contains 10 putative transmembrane regions and is inner-membrane-located." WHERE IS THE PROOF? A PUTATIVE REGIONS WHICH ARE SIMILAR TO OTHER STUDIED PROTEINS ARE NOT TO BE CONSIDERED AS PROOF FOR PROTEIN FUNCTION. THE DATABASE ANALYSIS OF PROTEINS ARE MERELY A CLUE TO THE REAL PROTEIN FUNCTION AND NO CONCLUSIONS CAN BE CONCLUDED FROM ANY FORMAL SIMILARITY OF THE GENETIC CODE AND ANY KNOWN PROTEINS. THE MOTIFS ARE NOT PROOF BUT SPECULATIONS. REAL PROOF = CRYSTALLIZATION AND POSITIONING OF GFP FOLLOWINF MICROSCOPY. Channels/ carriers NO ACTIVE CO2 CHANNELS ARE EXPECTED. THE OUTER CO2 is mmoles, and the photosynthesis is micromoles. THEREFORE, the DIFFUSION IS NOT ACTIVE. The "channels" are pipes which enhance membrane transport. The pipes are unspecific. The size of molecule is the only limiting factor. Large Surface area/ volume of bacteria, lead to the unnecessary existence of any channels in bacteria. CONCLUSIONS
  • 25. 25 1. The protein might be not a channel. 2. If there are channels in bacteria, there are also channels is chloroplast. 3. NO PROOF FOR FUNCTION 4. NO "CONCENTRATING MECHANISMS" EXIST 5. ACCLIMATION BETWEEN MEASUREMENTS UNMENTIONED. 6. NO PROOF FOR POSITIONING The Ci parameter is WRONG 11/15/2012 12:21:54 PM Mathematical function is set of points to compare at Y axis at similar X. The Ci parameter includes the photosynthesis. WHY TO INCLUDE TO X THE Y axis of the CI CURVE? The Ci curve is comparison of PHOTOSYNTHESIS VALUES AT DIFFEARENT Ci, When Ci includes already photosynthesis, resulting change in X values to different values. The X of the compared points is not same. COMPARISON IS WRONG. The calculation of Ci is artificial (not measured) calculation of a parameter similar to the measured parameter CO2R (External CO2). The true parameter which should be set to X axis is CO2R. COwR is the manually or automatically changed parameter and as function of CO2R changes in photosynthesis and transpiration do happen in the plant leaf. The artificial parameter Ci, calculates some a value close to CO2R, and based on Transpiration/ photosynthesis ratio, leaf temperature, and pressure. The artificial CO2 concentration is similar to CO2R, but less usually. The ERROR of Ci is that the CO2 concentration of the leaf is not related to STOMATA APERTURE. The diffusion of CO2 is barely limited by stomata. The only limitation of stomata is on transpiration. Therefore, the Ci is artificial and wrong. See "DECOUPLING OF PHOTOSYNTHESIS AND TRANSPIRATION AFTER ALTERATIONS
  • 26. 26 IN EXTERNAL CO2". ORANGE: IRRADIATION PURPLE: CO2 CONCENTRATION OF AIR GREEN: PHOTOSYNTHESIS BLUE: TRANSPIRATION Figure 1: DECOUPLING OF PHOTOSYNTHESIS AND TRANSPIRATION AFTER CHANGES IN CO2(Chart from Leonid Asipov MSc THESIS). The transpiration changes during a long time of 30 minutes or more after the change in CO2. Photosynthesis changes almost instantly(30 seconds), During the change in the transpiration rate, the photosynthesis does not change. During the period of change of the transpiration, Stomata change the aperture, process which does not affect the photosynthesis rate. The conclusion is that stomata mainly affect the transpiration rate due to differences in concentration between the leaf the air and slower speed of water diffusion (MORE LIQUID THAN CO2). CO2 diffusion is fast and almost not limited by stomata.
  • 27. 27 The BEST parameter is the real parameter CO2R, presented with Transpiration or Photosynthesis on separate curves. Ci unclear results : no conclusions EXTERNAL CO2 VS PHOTO
  • 28. 28 EXTERNAL CO2 VS TRANSPIRATION CONCLUSION : TRANSIRATION IS THE CAUSE FOR CHANGE OF CI , NOT PHOTO. .
  • 29. 29 L-Data vs "CO2 Concentrating Mechanisms in Algae: Mechanisms, Environmental" 11/15/2012 12:01:42 PM CCM mechanisms are not likely. CO2 is high concentration in the air, relative to plant. Artificially to elevate concentration is an impossible mission with an inefficient nature. Why to artificially elevate the concentration of CO2 in the chloroplast? The chloroplast's nature sugar consumption produces more CO2, thus elevating the CO2 concentration near the chloroplast. The discussed CO2 may be the reason scientists thought that the chloroplast possesses CO2 concentrating mechanisms. The nature works with diffusion. It is impossible to artificially change concentrations opposite to the substance concentrations. Water, salt, CO2, sugar, all substances diffuse naturally in the plant and the plant adapts to certain concentration of the substances. The citrated work does not prove existence of CCM, but states the CCM may exist. The science does not like science fiction. CO2 channeling. Channels are proteins which conduct particles according to the concentration gradient. The PIPES, are normal protein pipes. There are no transporters but PASSIVE, with GRADIENT. ENERGY IS MOVEMENT. MATTER DISSOLVING TO SMALLER PARTICLES ENLARGES HEAT CONDUCTANCE. CHAOS. THE MATTER IS STRUCTURE. THE CELLS CANNOT ENLARGE THE REACTIVITY( BY DISSIPATION OF SUGARS) AND EXPECT TRANSPORT OF SUBSTANCES AGAINST THE CONCENTRATION GRADIENT. THE DIFFUSION OF ONE SUBSTANCE IS BARELY RELATED TO THE OTHER. THE DIFFUSION IS RANDOM. THE DEPENDENCE IS ONLY AT HIGH CONCENTRATIONS, WHEN THE VOLUME IS A LIMITING FACTOR. THUS, NO ACTIVE TRANSPORTERS EXIST. REGULAR DIFFUSION ONLY. THE PLANT IS BUILDING THE ORAGNIZM WITH PARTICLES SUCH AS SUGAR, PROTEIN OR FAT, NO "ENERGY" EXIST
  • 30. 31 IN MATTER. SOME REACTIVITY (HIGH HEAT CONDUCTANCE DUE TO SMALL PARTICLES) IS NEEDED FOR DISSIPATION OF SUBSTRATE FROM ENZYME OR TEMPORARY LIFE OF BIO SUBSTANCES, WHICH IS VITAL FOR NEW CELL REBUILDING. L-Data vs: Development of synchronized, autonomous, and self-regulated oscillations in plant 11/14/2012 6:27:04 PM P.S I was a student of the publishers and the data abundant. Please consider the papers I enclose scientifically. SUMMARY NO SHORT TERM OSCILLATIONS OF PLANT TRASPIRATION EXISTENT. THE MEASUREMENT EFFECT IS DUE TO MEASUREMENT INACCURACY Flaws in the reported article. 1. SIMILAR OSCILLATIONS WITHOUT IMPORTANCE DAY/DARK 2. NO SHORT TERM OSCILLATIONS AT GAS EXCHANGE MEASUREMENT 3. OSCILLATIONS ARE SMALLER THAN THE NORMAL INACUURACY OF A ELECTRONIC SCALE: +- 2 grams (ACCORDING TO PRODUCER) Data from Tedea-Vishay 1040 C3 transducer. Total error : 0.02% of rated output. 0.02% of 5000 grams (average plant) is +- 1 gram. The total error of course is measured in ideal conditions of humidity, noise, temperature and wind. Normal experimental conditions of course Elevates the noise. THE NOISE OF WEIGHT SCALE IS AT LEAST +-1 gram.
  • 31. 31 Actual noise: +- 2 grams. 1. Inability to differentiate between oscillations due to fluctuation in light, temperature, humidity and water availability and the reported "spontaneous" oscillations. The reported experiments were held in a greenhouse with fluctuating irradiation parameter. The claim for "spontaneous" transpiration oscillations is impossible due to unstable environment. The control is a wet blanket positioned on a similar scale weight. The short term fluctuations of the control is without any significant correlation to plants, suggesting the short term fluctuations are not related to temperature or irradiation, but another sensor of transpiration (gas exchange of a single leaf) suggests the total absence of oscillations on basis of short term periods of less than 5 minutes. Since the temperature or light was not measured by any other sensor than the scale, an artifact can be speculated. The control should have been additional sensor such as light, temperature or humidity simultaneous to the weight measurement. The absence of a proper control to the experiment suggests improper scientific approach basing on a single device. The electronic scale has certain accuracy. The minimal accuracy stands for 0.3 % from the weight, and about +- 2 grams. The size of the reported oscillations is about +- 1 gram. Less than minimal accuracy, suggesting the short term oscillations are noise. 2. The weight measurement should have been held in controlled environment, to avoid any fluctuations of li8ght as effect on transpiration. Since the experiment was held in unstable light, the oscillations may be due to external irradiation fluctuations… The experimental setup Fig 1A: Whole plant on a weight scale for continuous transpiration measurement. Readings every 10 seconds, average to 3 minutes.
  • 32. 32 Fig 1B: Gas-exchange measurement of a single leaf. Points are measured every 2 minutes. The size of the oscillations is similar to normal measurement noise
  • 33. 33 Fig2: Weight scale measurement of a whole plant. Inaccuracies unseen since the total weight of the plant is much larger than the noise. Fig 3: Small noises are seen after differentiation since the transpiration value
  • 34. 34 between each point and the following point is only ~10 grams. The noise is 0.3% of total plant weight (about +-2 grams). Noise is 20% of measured value. Significance of results exists when value of transpiration is much larger than noise. On scale of 30 minutes the extent of the noise is 2.5%. After averaging on the range of 30 minutes, the oscillations are correlated with the irradiation levels (Fig 6). PAPER 1: DATA FROM MSC THESIS ACCEPTED BY THE CAMPUS OF REHOVOT. Simultaneous measurement of Gas-Exchange and Scales. Additional sensor. The scale is only one sensor. Artifact regarding the scale itself is to consider. The control of the scale is transpiration measured by gas-exchange method. The oscillations are expected to be larger, since a single leaf is less synchronized than the whole plant. The more stomata is measured, the less oscillations are expected, since the oscillations of single stomata is averaged by the experiment. The whole plant transpiration can be considered as NON oscillating PARAMETER. Experiment Setup 1. Load Cell A whole tomato plant is positioned on a load cell in a water container. The rate of transpiration is calculated from weight loss of the plant and the container. 2.Gas-exchange The rate of transpiration of the same plant is simultaneously measured using gas exchange method on a single leaf in the upper part of the plant. The experiment was made on a well watered plant during a whole day in natural light. Results
  • 35. 35 Fig 4: Simultaneous measurement of transpiration using load cell and gas exchange methods. Conclusions The oscillations are the similar size during the night and the day periods. The forecast is that during high transpiration period, the oscillations are supposed to be higher. However the oscillations, are not different. The fact that the oscillations are abundant during the night suggests that the oscillations are due to measurement artifact, which is basically inaccuracy of the weight scale. The accuracy is 0.3 % which is about +- 2 grams. The reported short term oscillations are about the same size, ~+-1 gram. The lack of any oscillations during the night period observed in gas-exchange measurement, suggests that the oscillations are an artifact related to the electronic scale. During the day period, there are long-term light-correlated oscillations seen also in gas-exchange experiment.
  • 36. 36 The fact that the experiment was held in natural light, adds uncertainty to the experiment. There is a degree of oscillations related to light fluctuations. The scale weight oscillations are hardly differentiated from light oscillations, however, the nocturnal oscillation of the weight suggests that the oscillations are an artifact. The better experiment is to measure in controlled environment, with constant light levels. The oscillations of a single leaf are expected to be larger than the oscillations of a whole plant. Light-Driven oscillations affect more higher leaves. The whole plant is less affected due to shading of leaves. The following chart consists of a smoothed (30 minutes moving average) weight scale experiment and a simultaneous gas exchange measurement. Chart 1: Simultaneous measurement of plant transpiration: Gas exchange – and scale. From Maters thesis Leonid Asipov, 2011. Fig 5:
  • 37. 37 1: Light (ORANGE) 2: Transpiration of a leaf(BLUE) 3. Transpiration of whole plant(DARK BLUE) Light: SUNLIGHT. LONG TERM FLACTUATIONS ARE CLEAR IN GAS EXCHANGE AND SCALES. Short term oscillations are random and night/day unspecific. Longer oscillations (0.5 hour) are related to fluctuations of irradiation levels. The pattern of the correlation is seen both in gas-exchange and scales experiment. Longer term oscillations are related to irradiation fluctuations. The short term oscillations seen on electronic scales measurement, are related to weight measurement inaccuracy. We would expect that oscillations in transpiration rate should be larger at daytime since transpiration is larger. The measured oscillations were found to be similar during DAY/NIGHT, suggesting the oscillations are not related to the transpiration rate but to spontaneous measurement noise. SHORT TERM OSCILLATIONS ARE ONLY SEEN AT ELECTRONIC SCALES MEASUREMENT OF A WHOLE PLANT. The significance of the measurement of the scales is when the transpiration is larger significantly than the measurement noise. The transpiration of 0.5 hour is 80 grams at maximum transpiration levels. The noise is 0.3 % which is about +- 2 grams. 2 grams is 2.5% of the measurement. The smaller the scale of consideration, the degree of noise is larger than the total transpiration during the period of time. The transpiration during 3 minutes is 8 grams. 2 grams of 8 is 25%. The extent of the spontaneous oscillations is large and the spontaneous noise on the scale of 3 minutes (the considered time scale of the reported article) is similar to the reported oscillations. Since the reported oscillations are similar to the spontaneous noise of the electronic scale, short time periods are not to be considered significant to the measurement. The measurement is considered to be significant when the extent of the transpiration is significantly larger than the noise. The time scale to
  • 38. 38 consider is larger than 0.5 hour. The total transpiration between the measurements is about 10 grams (3 minutes). The spontaneous inaccuracy is +-2 grams per measurement. 2 grams is about 20% of the measurement and is in the size of the reported oscillations. The weight is not to be considered on short time scale, when the total transpiration is not significantly larger than the spontaneous noise. The suggested time scale is not less than 0.5 hour, when the noise is about 2.5% of the total transpiration. The short term fluctuations are artifact. Fig 6: Measurements each 10 sec, averaged to 3 minutes. Y Axis: weight loss [grams/sec] X Axis: Time of day PURPLE: Transpiration without smoothing GREEN: Smoothing 13 points RED: Smoothing 25 points "The term" short term oscillations are related to fluctuations of transpiration on scale of less than 3 minutes. The short term oscillations are considered an artifact of the electronic scale. Transpiration and Photosynthesis measurement in controlled environment: stable transpiration parameter, changes due to CO2 changes in the air.
  • 39. 39 GAS EXCHANGE METHOD SHOW NO SHORT TERM OSCILLATIONS IN CONTROLLED ENVIRONMENT 1. Gas-exchange measurement of photosynthesis and transpiration in controlled environment: no short term oscillations, transpiration responds to changes in CO2. Fig 7: 1: orange Light levels. 2. Purple CO2 Concentration 3. Green Photosynthesis 4. Blue Transpiration of a tomato plant is controlled room. Measured by gas-exchange method.
  • 40. 41 Fig 8: Gas-exchange measurement of photosynthesis and transpiration in controlled environment: no short term oscillations, transpiration responds to changes in CO2. Similar experiment Gas exchange method works on a single leaf. The speculated oscillations should have been larger since synchronization of oscillations is hard to imagine. Nevertheless, no short term oscillations in plant transpiration are evident in gas- exchange measurement of transpiration. CONCLUSION: THERE ARE NO SHORT TERM OSCILLATIONS IN PLANT TRANSPIRATION L-Data vs “The Role of Tobacco Aquaporin1 in Improving Water Use Efficiency, Hydraulic Conductivity, and Yield Production Under Salt Stress1” 11/15/2012 11:34:24 AM
  • 41. 41 Rationale: Expression of an additional aquaporin improves water transport of the plant. Improvement of the water transport elevates the transpiration levels. The photosynthesis levels might elevate a little, however the main effect of stomata are on transpiration. Fig 1: 1: light levels 2. CO2 concentration 3. Photosynthesis 4. Transpiration Gas exchange experiment in controlled environment. Tomato photosynthesis and Transpiration rates during changing CO2 concentrations. Result 1. Fast acclimation of photosynthesis to CO2 alterations and constant photosynthesis rate during the stomata aperture change. Conclusion
  • 42. 42 1. Stomata barely affect photosynthesis. 2. The negative effect of salt on photosynthesis is not result of stomata closure (which does not change the abundant CO2 concentration), but due to the direct effect of salt on the plant photosynthesizing enzymes. 3. Enhanced water transport may improve enzymatic rates ONLY at salt stress conditions. The more open stomata due to the expresses aquaporin, are supposed to enhance transpiration but not elevate photosynthesis at normal (NOT SALT) conditions, since stomata aperture is not related to the photosynthesis rate(Fig1). The reported WUE enhancement of the transgenic plants is absurd, Water use efficiency: Total dry plant to unit of water weight. Plant dry weight [kg] /WATER [kg] The transpiration rate is about 70 times faster (per mole water relative to mole CO2) than photosynthesis. In order to preserve constant WUE at elevation of 15% in transpiration levels, which is seen in the transgenic plants, Photosynthesis has to be elevated 10 times (1000%) 70 + 15% of 70 = 80 photosynthesis units. The molar weight of water is 19 and the assimilation of a single C atom is only 6 gram/mole. The O2 is returned back to the air and thus, at single molar measurement of photosynthesis and transpiration additional 300% of the elevation in transpiration rate to be added to the photosynthesis rate in order to preserve constant WUE. 70 + 15% of 70 + 3* 15% of 70 = number of photosynthesis units needed for CONSTANT WUE = 42 times more photosynthesis needed to preserve constant WUE at 15 % more transpiration. The duration of transpiration is the whole day, while the photosynthesis is abundant only at day time. Therefore the total daily transpiration of the transgenic plants is elevated MUCH more than the total photosynthesis.
  • 43. 43 The reports regarding the elevated WATER use efficiency of the transgenic plants are not logical. Every percent of elevation in transpiration levels is actually more than 3 times more water per unit of dry weight. The amount of CO2 molecules per single water is 70, meaning that elevation in transpiration is serious to WUE. For a single percent of added transpiration 300% of photosynthesis is needed to keep constant WUE, otherwise WUE declines. The cells which are full in water are larger in size, and thus the time CO2 diffuses is longer. The longer time of diffusion causes usually decline in photosynthesis rates in highly irrigated plants. The extent of photosynthesis addition of the transgenic plants is incomparable to the extent of used water. The improvement of 50% in photosynthesis is only in peak irradiation hours and salt stressed plants. To elevate WUE photosynthesis has to elevate 45 and not 2 times. NO ELEVATION IN WATER USE EFFICIENCY IS EVIDENT EVEN IN THE TOP LIGHT and SALT STRESS CONDITIONS, when the aquaporin is the most affecting the plant. During normal conditions the differences in photosynthesis are not large, however the difference in transpiration in steady on ~16% from wt. Note that the plant transpires all the day and not only at peak light hours. Inconsistency of the results measured by gas-exchange and whole plant scale measurement. Figure 3 presents larger transpiration rates to transgenic plants both at salt and normal irrigation. Figure 2 presents transpiration results of whole plant. The whole plant transpiration of transgenic plants is lower than WT. Aquaporins
  • 44. 44 improve water transport, and thus more transpiration is expected. HOW THE AUTHOR EXPLAINS THE INCONSISTENCY? WHY IS PHOTOSYTHESIS ON NORMAL IRRIGATION IS NOT PRESENTED? The effect of the aquaporin is significant only when there is water deficit. At normal irrigation, the photosynthesis levels of Transgenic and wt plants should not differ significantly. The reason of the aquaporin effect on photosynthesis is that the photosynthesis is reduced by high salt concentration during salt stress. Moreover, the transgenic plants transpire more water and thus get faster to drought stress. The addition of the aquaporin enhances water transport, which is positively affecting photosynthesis only at salt stress conditions, when the salt levels start to block enzymatic processes. CONSTANT LIGHT DURING THE GAS-EXCHANGE EXPERIMENT The light during the experiment was artificial and constant. The reason for high transpiration at noon hours is temperature not irradiation. Due to the artificial light, the photosynthesis was elevated during the whole measurement period (morning=>noon), which would have not been expected with natural light, which is at PEAK only few hours a day. When light is less intense, differences in photosynthesis rates are smaller. The LIGHT levels was 1200uE which is stronger intensity relative to real conditions. The light was TOO high in order to intensify the photosynthesis rates of plants and enhance the effect of the aquaporin on salt stress. LESS LIGHT would have declined the TOTAL photosynthesis and thus WUE would have been declined. TOO HIGH LIGHT is UNHONEST EXPERIMENT. The real light is lower, not only to the top leaves but to the rest of the plant. Most of the plant is at much lower light levels (~200uE). The differences in photosynthesis rates due to better water transport during salt stress would have declined to ~5-10 % at maximum relative to the reported 50%.
  • 45. 45 Note that the transpiration of the transgenic plants is elevated at all conditions to more than 15%. At real conditions, the light levels are much lower, regarding the fact that at morning and evening hours the light is very low intensity. However, transpiration levels are related to temperature and are not maximum, since the temperature of the air is related to the real irradiation. CONSTANT LIGHT DURING EXPERIMENT IS A FRAUD. THE TEMPERATURE OF THE AIR ALTERS TRANSPIRATION, BUT PHOTOSYNTHESIS IS KEPT MUCH MORE CONSTANT DUE TO CONSTANT LIGHT (NOT ENTIRELY CONSTANT DUE TO TEMERATURE WHICH INCREASES THE PHOTOSYNTHETIC REACTIONS AND DIFFUSION). THE PHOTOSYNTHESIS CURVE WAS NOT PRESENTED MOST POSSIBLY DUE TO CONSTANT LIGHT WHICH DOES NOT CORRELATE TO THE CHANGING TRANSPIRATION. NATURAL LIGHT DID NOT AFFECT PHOTOSYNTHESIS, ONLY TRANSPIRATION.
  • 46. 46 Fig 5: Typical irradiation levels in a greenhouse with natural light. UPPER LEAVES. Rest of the plant, LESS than the following. Average irradiation of morning: 450-500 uE, average irradiation of Noon : 750uE The reported experiment : constant 1200uE from morning to evening. The publisher enhances photosynthesis, which is enhanced by the aquaporin at SALT STRESS. At real light conditions there is less photosynthesis due to lower light levels. The transpiration levels, however are kept according to the air temperature and are influenced by the changing irradiation levels. The real light is HOT, and the artificial light is cold. 1200uE of SUNLIGHT would have elevated transpiration significantly. 1200uE of artificial light, is constant, from morning to noon and transpiration is related to air temperature affecting on the whole plant. The effect of irradiation on transpiration is reduced due to the cold artificial light of the measurement chamber. The researcher accents photosynthesis and tries to hide transpiration. In order to
  • 47. 47 report POSITIVE WUE of transgenic plants. HOWEVER TO IMPROVE WUE, photosynthesis has to rise by 45 times of the elevation of transpiration, during the whole day Period due to mass differences of water and Carbon and molar differences of transpiration and photosynthesis. The SALT STRESS EXPERIMENT Salt ACCUMULATION in plant force plants to grow BAD on water with high salinity. The more plants transpire, faster the accumulation of salt, and therefore the plants live shorter lives until the plant death. Growth and fruit count declines. The transgenic plants are more sensitive to salt on the longer run due to salt accumulation. The addition of salt during the experiment is not a good model for NATURAL field conditions. The fields which absorb too much salt for plant growth should be replaced with new soil. Drought stress is the real experiment to test new genetically engineered plants. The drought stress is predicted to be a failure for the transgenic plants carrying the aquaporin which causes more transpiration and thus the plants get stressed before the wt plants. Elevated transpiration of the transgenic plants DECLINE WUE causing drought sensitive plants or LARGER WATER DEMAND. The improvement of photosynthesis is ONLY at SALT STRESS CONDITIONS WHICH ARE NOT REAL. BETTER SOLUTION THAN GENETIC ENGINEERING IS REPLACING THE SOIL. Conclusions 1. WUE of the transgenic plants to decline since the differences in photosynthesis rates are not high enough relative to differences in transpiration. 2. The transpiration of the whole plant is inconsistent with transpiration of a single leaf (ERROR IN FIGURE?).
  • 48. 48 3. The effect of stomata aperture on photosynthesis is small. The effect of the aquaporin enhances the photosynthesis mainly at salt stress conditions, when enzymes has not optimal concentration of solutes. Better water transport elevate photosynthesis. The transpiration is mostly related to stomata aperture. The effect of the water on photosynthesis is not related to stomata, otherwise we would expect similar or smaller effect on photosynthesis. 4. The transgenic plants are salt stress sensitive due to salt accumulation. 5. The transgenic plants are drought stress sensitive due to higher transpiration rates. 6. The LIGHT levels during the gas-exchange experiment was constant, larger and colder than natural light. The fact enhances photosynthesis, while transpiration is reduced. SUNLIGHT is hot irradiation which enhances transpiration. The elevation of transpiration during the day is due to temperature changes in the greenhouse. The constant light is UNHONEST SCIENCE, since the air temperature was not kept constant (SEEN AS UNCONSTANT TRANSPIRATION LEVELS). The reason for the lack of photosynthesis after dehydration is lack of water for enzymes 30/12/2012 18:51:51 Transpiration and photosynthesis during three dehydration days. Note that day 2 is less water for plants and nevertheless there is more transpiration and same photosynthesis. The stomata close. Same photosynthesis : there is no relation of stomata and photosynthesis: enough water = same photosynthesis. Transpiration is higher due to higher leaf temperature and less water elevation by the roots thus less leaf cooing by transpiration...
  • 49. 49 WUE CANNOT GROW 10/9/2012 11:57:08 AM CRITICISM ON : "The role of tobacco aquaporin 1 in improving water-use efficiency, hydraulic conductivity and yield production under salt stresses. " WUE OF PLANTS EXRESSING AQUAPORIN CANNOT GROW. MORE WATER FOR TRANSPIRATION ALL THE TIME. PHOTOSYNTHESIS HAS TO DECLINE DUE TO MORE WATER IN CYTOPLASM. MOR DIFFUSION TIME. THE OPEN STOMATA ARE NOT SUPPOSED TO ENHANCE PHOTOSYNTHESIS DUE TO HIGH SPEED OF DIFFUSION OF CO2. NEVERTHELESS, THE PERIOD OF LIGHT IS SHORT AND THE TRANSPIRATION ELEVATION IS FOR LONG TIME. THE TOTAL WUE HAS TO DECLINE DUE TO TRANSPIRATION ELEVATION. THE RESULTS SUGGEST ELEVATION OF WUE but ONLY FOR A TOP LIGHT STRENGTH AND SHORT PERIOD (GAS EXCHANGE, ARTIFICIAL LIGHT AND TOP CO2 CONCENTRATIONS). L-Data vs "SYNERGISM OF LIGHT AND FUSICOCCIN ON STOMATAL OPENING"
  • 50. 51 11/15/2012 12:03:31 PM Stomatal opening in FC or KCI. How possible that higher concentration of KCL open the stomata and not closing? High concentration, water exits the stomata and the stomata closes. 2. Why to couple 2 effects: solute concentration and light? Light causes photosynthesis and thus decline in CO2 concentration. When CO2 concentration is below threshold of stomata opening, the stomata start to open. Solute concentration is effect unrelated to light. L-Data vs "Changes in the properties of reaction center II during the initial stages of photo inhibition as revealed by thermo luminescence measurements" 11/15/2012 11:56:53 AM Leaves of plants exposed to intense light accumulate more light particles. At lower temperatures than usual, the particles will be depleted off the plant tissue. The switch of the band from higher temperature to 15% is due to more light particles accumulated in the leaf. Phosphorus is the main matter accumulating light and producing phosphorylation. The concentration of phosphorus is the main factor of phosphorylation, as measured by TL system. The TL is mainly a phosphorus meter. Additional light absorbing substances are also existent. A regular spectrophotometer can usually replace TL. Photo inhibition is due to destruction of plant leaves by excessive irradiation. The cure: shading. CO2 not light is the trigger for leaf conductance to gases
  • 51. 51 1/9/2011 11:16:47 AM Introduction Changes in light levels or air CO2 concentration cause changes in leaf conductance to gases. I: Leaf conductance changes in response to changes in air CO2 concentration : the response happens in darkness or light. Conclusion The response ton changes in CO2 concentration is light independent. This suggests that CO2 is a trigger which inflicts on leaf conductance to gases. In light the effect is stronger due to photosynthesis, which lowers the CO2 concentration near and inside the leaf. II: Direct effect of light 1. High Light
  • 52. 52 2. Low light Conclusions The effect of light on leaf conductance (which we see as transpiration rate) after turnover from darkness is not immediate, and gets larger with lower light levels. During this response time, the COI2 levels in the leaf intercellular space gets below certain threshold after which an effect on transpiration is visible. In higher
  • 53. 53 light levels thr transpiration response time shortens significantly as intercellular CO2 threshold is reached quicker in faster photosynthesis levels. The fact that the effect of light on leaf conductance to gases (as seen in transpiration) is not immediate and related to the gradual reduction in the CO2 concentration in the intercellular space, in addition to light independent response of the leaf conductance to gases to changes in CO2 concentration in the air, suggests that CO2 is the primary trigger of the leaf conductance to gases, while light plays an indirect role as Photosynthesis inducer. Materials and Methods Data-Lightning universal platform for data analysis, L-Data. There is no main growth control in plant : transpiration is related to nutrient transport 8/28/2011 9:41:40 AM ‫של‬ ‫אין‬ )‫חלבונים‬ ,‫(פחמימות‬ ‫בנייה‬ ‫חומרי‬ ‫של‬ ‫מדפוזיה‬ ‫נובעת‬ ‫שרואים‬ ‫התופעה‬ .‫בצמח‬ ‫קודקודי‬ ‫טון‬ ‫טרנספירציה‬ ‫גם‬ ‫ולכן‬ ‫פוטוסינטזה‬ ‫שמבצעים‬ ‫בצמח‬ ‫לעלים‬ . ‫המים‬ ‫של‬ ‫הריכוזים‬ ‫מפל‬ ‫עם‬ ‫תמידית‬ ‫מים‬ ‫של‬ ‫זרימה‬ ‫יש‬ ‫טרנספירציה‬ ‫כשיש‬ . ‫מורידה‬ ‫טרנספירציה‬ ‫לעלה‬ ‫מים‬ ‫של‬ ‫זרימה‬ ‫יש‬ ‫ולכן‬ ‫המןמסים‬ ‫ריכוז‬ ‫את‬ . ‫לאט‬ ‫לאט‬ .‫לעלה‬ ‫מים‬ ‫של‬ ‫זרימה‬ ‫אין‬ ‫ולכן‬ ‫טרנספירציה‬ ‫ללא‬ ‫נמצאים‬ ‫פוטוסינטזה‬ ‫מבצעים‬ ‫שלא‬ ‫עלים‬ ‫מדי‬ ‫איטית‬ ‫הסוכרים‬ ‫ואספקת‬ ‫הסוכרים‬ ‫נגמרים‬ . ‫אספקה‬ ‫מהעדר‬ ‫נושרים‬ ‫בחושך‬ ‫ושנמצאים‬ ‫פוטוסינטזה‬ ‫ללא‬ ‫העלים‬ . ‫תאים‬ ‫של‬ ‫למוות‬ ‫גורמים‬ ‫סוכרים‬ ‫עודף‬ .‫סוכרים‬ ‫מעודף‬ ‫נובלים‬ ‫שנתיים‬ ‫חש‬ ‫צמחים‬ ‫מאוסמולריות‬ ‫מהתייבשות‬ ‫מת‬ ‫והצמח‬ ‫גבוהה‬ . ‫בעתיד‬ ‫משתלמת‬ ‫אבל‬ ‫אנרגטית‬ ‫השקעה‬ ‫שעולה‬ ‫וגדילה‬ ‫אנרגייה‬ ‫ייצירת‬ ‫הם‬ ‫צמח‬ ‫של‬ ‫חיים‬ ‫תחילת‬ . ‫מנת‬ ‫על‬ ‫מספיק‬ ‫מייצר‬ ‫לא‬ ‫עצמו‬ ‫העלה‬ ‫כי‬ ‫לגדילה‬ ‫אספקה‬ ‫מקבלים‬ ‫באור‬ ‫שנהנצאים‬ ‫וענפים‬ ‫עלים‬ ‫רק‬ ‫הצמח‬ ‫כל‬ ‫של‬ ‫משותפים‬ ‫ממשאבים‬ ‫הגדילה‬ ,‫לגדול‬ . ‫רי‬ ‫כשיש‬ ‫הפחמימות‬ ‫ריכוז‬ ‫את‬ ‫לווסת‬ ‫מנת‬ ‫על‬ ‫לגדול‬ ‫רוצים‬ ‫עלים‬ / ‫הענפים‬ ‫כל‬ ‫אנרגייה‬ ‫של‬ ‫גבוה‬ ‫כוז‬ . ‫האנרגייה‬ ‫את‬ ‫משקיע‬ ‫הצמח‬ .‫פירות‬ ‫גידול‬ ‫מתחיל‬ ,‫מסויים‬ ‫ריכוז‬ ‫מעל‬ ‫גדל‬ ‫הפחמימות‬ ‫שריכוז‬ ‫בשלב‬ ‫ולא‬ ‫קבוע‬ ‫ם‬ ‫מומסי‬ ‫ריכוז‬ ‫על‬ ‫לשמור‬ ‫מנצת‬ ‫על‬ ‫בפירות‬ ‫שפירות‬ ‫בסוף‬ .‫לסף‬ ‫מעבר‬ - ‫אפשרות‬ ‫אין‬ ‫גדולים‬ ‫האנרגייה‬ ‫וריכוז‬ ‫אנרגטית‬ ‫להשקעה‬ ‫לצמח‬ ‫אחרת‬ ‫המקסימלי‬ ‫לסף‬ ‫מעבר‬ ‫עולה‬ ‫בצמח‬
  • 54. 54 ‫הצמח‬ ‫והתיישבושת‬ ‫תאים‬ ‫של‬ ‫למוות‬ ‫שגורם‬ ‫מה‬ . ‫נוע‬ ‫הדבר‬ .‫הימוח‬ ‫אנרגיית‬ ‫את‬ ‫מקבלים‬ ‫העליונים‬ ‫והענפים‬ ‫שהעלים‬ ‫בכך‬ ‫מתבטא‬ ‫הקודקודי‬ ‫השלטון‬ ‫טרנספירציה‬ ‫ומבצעים‬ ‫באור‬ ‫המצאים‬ ‫שהם‬ ‫מכך‬ . ‫יק‬ ‫גורמת‬ ‫טרנספירציה‬ .‫לצימוח‬ ‫שטובים‬ ‫והחלבונים‬ ‫הסוכרים‬ ‫לאספקת‬ ‫ולכן‬ ‫המים‬ ‫בפוטנציאל‬ ‫רידה‬ ‫קודקודי‬ ‫שלטון‬ ‫של‬ ‫נוספת‬ ‫תופעה‬ - ‫אם‬ ‫הציד‬ ‫גידול‬ ‫ראשית‬ ‫מריסטמה‬ ‫להעדר‬ ‫קשורה‬ ‫התופעה‬ .‫קודקוד‬ ‫אין‬ - ‫מנוצלת‬ ‫האנרגייה‬ .‫צימוח‬ ‫אפשרות‬ ‫אין‬ ‫אחרות‬ ‫במריסטמות‬ . ‫טרנספירציה‬ ‫פחות‬ ‫צריך‬ ‫כך‬ ,‫אנרגייה‬ ‫יותר‬ ‫שיש‬ ‫ככל‬ ‫אספקה‬ ‫לקבלת‬ , ‫שעשיר‬ ‫צמח‬ ‫של‬ ‫במצב‬ ‫כך‬ ‫באנרגייה‬ - ‫הצידה‬ ‫גם‬ ‫היא‬ ‫הגדילה‬ . Cactuses create sugar during the day 9/8/2011 3:39:39 PM
  • 55. 55 Cactuses create sugars from CO2 and water. During the night, all the gas exchange takes place. The stomata open, the O2 from the day irradiation diffuses to the air and CO2 diffuses to the plant. The plant structure is low surface area/volume ratio and accumulates large amount of CO2 and O2. A the morning stomata close and irradiation start creating O, H and C from H2O and CO2. The O2 is accumulated in the plant leaves, and CO2 is the accumulated CO2. The sugar is enzymatically formed during the day, while the basic components are created. As soon as stomata are opened during the night, gas exchange takes place. CO2 diffuses to the plant, where CO2 concentration is low. O2 diffuses to the air. Without irradiation, the basic components are becoming back to CO2 and H2O. During the night only gas exchange takes place. There is no CO2 fixation : O2 concentration blocks photosynthesis by reverse CO2 formation
  • 56. 56 9/11/2011 6:54:38 PM There is no CO2 fixation. CO2 diffusion from the air brings CO2 to cells, then light break the two bonds of CO2 and leaves C in the cells. O2 is diffused back to air. The effect of O2 on photosynthesis is C returning back to O2 and CO2 reforming. On higher O2 concentrations, the reaction of C creation is reversed, and thus, no visible photosynthesis takes place (photosynthesis is measured by CO2 concentration change in time unit). The formation of C from CO2 is light driven, not enzyme driven. The next stage is enzymatic formation of sugar. Stomata don't affect photosynthesis 9/18/2011 9:59:52 AM Stomata aperture don't affect photosynthesis, only transpiration. Decoupling of photosynthesis and transpiration rate after CO2 changes, has to be explained. The difference of the effect is due to high water concentration difference between the plant and the air. CO2 concentration difference is not high, due to respiration of the plant. The stomata aperture mainly affect not photosynthesis.
  • 57. 57 CO2 Channels Have to Enlarge Transpiration 9/25/2011 4:19:05 PM CO2 channels have to enlarge transpiration. Any channel which conduct molecules with size larger than water, would conduct water. If Such channels exist, they would have to conduct water. Over expression of CO2 channels would enlarge transpiration. CO2 channels are static like every channel. Since no charge exist, the channels are only size limiting. Whether if they exist we don't know. An possibility is that water channels conduct CO2 also, they have a size larger than water molecule. The stated protein was said to lower transpiration. If the protein, as stated is a CO2 channel, transpiration would have to be elevated.
  • 58. 58 That is the reason for absence of any physiological effect of stated proteins on photosynthesis or transpiration. Human industry is nature's best friend 10/23/2011 11:04:39 AM Humans are friends of nature. Without humans, nature would have run off of CO2. Bacreria which create oil, take CO2 and create solid or liquid matter. CO2 is off circulation. Humans found oil, and coal and crated industry and engines, while returning CO2 back to atmosphere. With no humans nature would have disappeared, plants would have no CO2 to produce sugars and animals would have no food. CO2 circulation due to industry elevate crops and saves water. Humans and modern culture of industry is best friend of nature. No need for CO2 channel for chloroplast 1/18/2012 9:19:29 AM
  • 59. 59 L-Data vs Rapid assessing of water and nitrogen status in wheat flag leaves. NOT N, possibly only water is reason for difference in weight of wheat 10/10/2012 3:00:42 PM "Near pipes there is more water and N. Weight of wheat is higher. The authors wrote N is causing the weight. WHY NOT IRRIGATION? TRAIT OF WEIGHT ON FIELD PIPE.AND Physics Light Speed is not Constant Light speed is not constant. Light particles have mass, and like everything with mass
  • 60. 61 are subject to gravity interactions with other masses. The light particles are emitted from the sun in process of thermonuclear processes and have high speed and high energy (much higher than visible light). In the process of light travel in space there is constant gravitational force of the sun on the light particles, which slow down and with their arrival to earth they are less energetic and mostly in visible light spectrum. The light rays are not straight too, due to mass effect by planets from the side during their travel in space. Relativity Theory Wrong 5/11/2011 7:12:57 PM Relativity theory wrong: Matter is not relative. Gravitational interactions between masses
  • 61. 61 exist without dependence of other factors. The only two natures exist in the world is mass (matter) and its movement. Relative phenomena is movement of masses. Speed is rate of change of position distance/per time = movement of mass relative to another mass. But not matter itself: it is not relative. ‫משוגע‬ ‫היה‬ ‫ידוע‬ ‫מדען‬ ‫יחסי‬ ‫אינו‬ ‫החומר‬ ‫תנוע‬ = ‫והאנרגייה‬ ‫חומר‬ ‫של‬ ‫ה‬ ‫מחומר‬ ‫תנועה‬ ‫ליצור‬ ‫ניתן‬ ‫כביכול‬ ‫שלפיה‬ ‫דבילית‬ ‫נוסחא‬ ‫כובת‬ ‫המדען‬ . ‫הידועה‬ ‫הנוסחא‬ = ‫בטלוויזיה‬ ‫שודר‬ ‫אפילו‬ ‫שלאחרונה‬ ‫דביליזם‬ . ‫לעבור‬ ‫צריך‬ ‫שחומר‬ ‫לכך‬ ‫נגרר‬ ‫יחסי‬ ‫שהכל‬ ‫התחלתית‬ ‫הכרזה‬ ‫על‬ ‫מגונן‬ ‫בעודו‬ ‫המדען‬ = ‫ותנועה‬ ‫חומר‬ .‫נפל‬ ‫המדען‬ ‫למעשה‬ .‫המדען‬ ‫נופל‬ ‫אחרת‬ ‫לתנועה‬ ‫שונים‬ ‫מושגים‬ ‫שני‬ . Matter does not have inner structure 5/16/2011 5:25:55 PM Matter does not have an inner structure: inner structure was historically to explain magnetic and electrostatic forces. No inner structure is better perspective: all the particles have affinity to one another. Electrostatic forces are due to the effect of elevation in gravitational force in smaller masses due to surface area/volume ratio. The smaller particles have higher affinity and thus electrostatic forces are created. Magnetic force is due to spontaneous exit of particles from the conductor. There is no minimum speed needed to fly to space 8/17/2011 1:54:32 PM ‫מהי‬ ‫שצריך‬ ‫טענו‬ ‫מדענים‬ ‫לאורביט‬ ‫מסה‬ ‫ליציאת‬ ‫מינימלית‬ ‫רות‬ ‫ה‬ . ‫דלק‬ ‫מספיק‬ ‫שיש‬ ‫בתנאי‬ ‫איטית‬ ‫במהירות‬ ‫אפילו‬ ,‫הארץ‬ ‫כדור‬ ‫של‬ ‫המשיכה‬ ‫מכוח‬ ‫לצאת‬ ‫ניתן‬ . ‫הדלק‬ ‫תצרוכות‬ ‫עולים‬ ‫ולכן‬ ‫המשיכה‬ ‫כוח‬ ‫מול‬ ‫להחזיק‬ ‫זמן‬ ‫יותר‬ ‫בהרבה‬ ‫צורך‬ ‫יש‬ ‫איטית‬ ‫במהירות‬ ‫הרבה‬ ‫ששוקל‬ . ,‫קל‬ ‫דלק‬ ‫עם‬ ‫מנוע‬ ‫ויש‬ ‫במידה‬ ‫מהירות‬ ‫בכל‬ ‫לחלל‬ ‫לטוס‬ ‫ניתן‬ . ‫לכדור‬ ‫המסה‬ ‫של‬ ‫לחזרה‬ ‫תגרום‬ ‫מספיקה‬ ‫לא‬ ‫מהירות‬ .‫הארץ‬ ‫לכדור‬ ‫במשיק‬ ‫מהירות‬ ‫דורשת‬ ‫אורביטה‬ ‫הארץ‬ ,
  • 62. 62 ‫הגורם‬ .‫נדרשת‬ ‫מינימלית‬ ‫מהירות‬ ‫ואין‬ ,‫המשיכה‬ ‫מכוח‬ ‫ולצאת‬ ‫לחלל‬ ‫לטוס‬ ‫ניתן‬ ‫מהירות‬ ‫בכל‬ ‫אבל‬ ‫דלק‬ ‫הוא‬ ‫המגביל‬ ‫בצורה‬ ‫הסבירות‬ ‫לא‬ ‫המדענים‬ ‫וכך‬ ‫המהירת‬ ‫לא‬ ‫של‬ ‫המשיכה‬ ‫מכוח‬ ‫יציאה‬ ‫של‬ ‫ט‬ ‫הקונצפ‬ ‫את‬ ‫נכונה‬ ‫הארץ‬ ‫כדור‬ . Gravitational force is related to Surface Area/Mass ratio 8/21/2011 2:34:33 PM Gravitation of small particles Gravitational force has direct relation with mass surface area/volume proportion. Force create acceleration, which is directly related to mass. The surface area is the area on which a collision with the gravitation particle can happen. Thus the relation of the gravitation force has to be F= SurfaceArea/Mass. Smaller masses have relatively higher gravitational effect but only on collision with gravitation particles. Without collision, they travel farther,
  • 63. 63 due to lower collision chance. Irradiation consists of large amount of particles and thus many of them travel without any collision. The more surface area a mass has, the more chance is to collide with the gravitation particle. And thus, larger masses have larger visible effect of gravitation. High speed of particles with large mass can make collision with gravitation particles not to be in high effect. If masses relatively larger than a single gravitation particle are considered, the frequency of collisions with the gravitation particle is higher to mass unit in masses with high surface area/ mass ratio relative to masses with lower surface area/mass ratio. Smaller masses have higher gravitational forces with proportion to the mass. This is correct to relatively large particles, such as atoms. Smaller particles such as photons, electrons have lower chance for collision with gravitation particles and therefore the effect of gravitation is less than for larger particles. This is seen is large distance travel of photons, and wire skipping of electrons following electric force. The particles are not of right form. Sand, salt and any other materials 8/23/2011 12:52:48 PM The particles are not of right form. Sand, salt and any other materials under the microscope show differences in particle size, form and generally without proper form. We speculate that the phenomena is also on microscopic level of atoms. They vary in size, mass, their properties are due to differential concentration and therefore hardness which is related to the time the matter was concentrating by the gravitational forces. The world is not consisting of even size or right form particles. All the particles are different, and the distances between them are various. The gravitational force concentrate the particles and gradually change
  • 64. 64 their form until gaps disappear. There is no possibility of creating ideal space 8/28/2011 10:05:49 AM There is no possibility of creating ideal space. Every space is particle collection. To create space, a flow of smaller particles is sent through an area with larger particles. The smaller particles are pushing the larger ones off an are but themselves are spread in the area. During the whole time of the flow of the small particles some particles flow in opposite direction.
  • 65. 65 Classical energy equation is wrong : energy is speed/mass 9/11/2011 6:46:20 PM Energy is speed/mass, not the usual equation. All there is is movement to mass. The classic equations are wrong. In mechanical system, movement/mass is constant. Masses transfer movement/mass to each other at collision. Since all there is is matter and speed, speed/mass is the only possible energy parameter.
  • 66. 66 The parameter which change matter properties is mass/volume, matter concentration. Movement of masses is enough to explain all physical or chemical phenomena 8/21/2011 12:53:25 PM There is two natures in the universe: mass, which is matter, and it's movement, which is speed. On micro level small particles of matter, without any further structure construct all the universe we see, including light, air, water, hard material or magnetic fields. The nature of forces: Gravitational force
  • 67. 67 The nature of forces between the particles is constant flux of tiny particles from many directions on every mass. The collision of small particles and the other masses create the gravitational force on every mass. Small or big masses are affected by the gravitational particle flux. A field is a cloud of particles moving in certain speed. In the area of the field masses are affected by collisions of the particles of the field. Gravitational particles create gravitational field. The force is not related directly with the masses, it is caused by flux of tiny particles from the outer side of the masses. Magnetic Fields. The nature of magnetic fields is flux of electrons from the wire. Some electrons exit the wire from the side, the gravitation force affect the particle from the side until the direction is around the wire.
  • 68. 68 The magnetic force is created by collision of electrons with the mass affected by the force. Activation energy: chemical bonds, Temperature, Exothermic and Endothermic reactions The effect of temperature is related to frequency and power of collisions of particles on a surface. Chemical reactions require energy which is minimal speed of particles needed for proper collision and chemical bond creation. Less than the minimum speed won't stand against the gravitational force and the back flow of gravitation particles from every atom. Proper collision partially disrupt the structure of the atoms creating tiny particle cloud around the area of the collision. The gravitation particle flux concentrate the cloud until creation of strong area which is called chemical bond. On collision, the two particles lose speed and therefore the reaction causes a temperature decline, due to reduction of the frequency of collision of the particles on the surroundings. Exothermic reaction is reaction with elevates the temperature
  • 69. 69 of the surroundings. This kind of reaction is disruption of chemical bonds. The activation energy needed for exothermic reaction is movement of other particles which have to collide with the bigger molecule until disruption of the chemical bond. On disruption of the chemical bond, smaller masses are created, which hit the surroundings with higher frequency. The higher frequency cause temperature elevation, or better conduction of kinetic energy from the atoms of the reaction to the surroundings. Atomic Bomb There is no ideological difference between the atomic bomb and a regular fire. In fire a molecule of some fuel is disassembled to atoms and the atoms collide with the bigger molecule. The collision create high speed movement of the small masses as well as evaporation of the matter. The high temperature is due to the high speed movement of the particles. Atomic bomb is the same process on the level of the atom. Uranuim atom is being partially destructed by a regular explosive. The tiny particles created collide with the big atoms and travel on
  • 70. 71 high speed to every direction. The particles create high temperature on collision with other masses. Hydrogen Bomb Hydrogen bomb is high speed movement of tiny particles created by collision between two hydrogen atoms. The collision is caused with collision of tiny particles created by an atomic bomb explosion. The two hydrogen atoms collide and some of the particles of the atom are released and collide with the bigger atoms. On collision the tiny particles gain high speed. The tiny particles travel on large distances because they are not collided with the gravitational particles. They create high temperatures on collision with other matter.
  • 71. 71 Electrical force, structure of metals Metals are constructed of atoms which are connected to each other. The connection is uniform. Between the atoms of the metal small ducts are created, in those ducts, electrons can travel from the high concentration to the low concentration, creating the possibility of electric power usage in their way. The electrons as well heat the wire by random collisions. Some of the electrons skip from the wire to the side, creating the magnetic field.
  • 72. 72 Gases, Liquids and Solids The difference is in matter concentration. Gases are consisted of small particles with large distances travelling on high speed. The particles can be rearranged closer to each other by speed reduction or in other words, temperature reduction. As soon as they are close, the gas is solid. The particles of the gas are still to as concentrated as solids. Solids consist of small particles which are very close to each other and thus the gravitational force is affecting from all sides and keep the concentration of the mass to maximum strength. The strength of matter is a sole result of closeness of the particles to each other and the phenomena is seen in metals. It is enough to press two pieces of metal to each other with strength if the surface is very straight. The two pieces are connected strongly and act like connected material. Since the atoms are with no right form (malformed structure) , it takes time to create solid matter, since gravitational particles collisions create the effect of matter solidification which is concentration of matter particles. The structure of the particles is slightly rearranged and the spaces between the particles disappear. As soon as this happed, the matter becomes strong. Spaces between the matter particles make weaker matter. Gases are matter with large spaces, liquids with smaller, solids with even smaller. The smaller distances between the particles of the solid, the stronger is the solid. Elevation of the speed of the particles can make deconcentration of matter, liquidation , or evaporation. Reduction of speed create more solid matter. The concentration of matter is made by gravitation particles constant flux on every mass.
  • 73. 73 Electromagnetic fields Electromagnetic field is a cloud of small particles travelling usually in high speeds. Light particles travel in high speed and affect other masses in the area of their travel. Conclusions The matter consists of tiny particles lacking any further structure. The matter properties such as strength or matter state (liquid, gas, solid) are a result of the distances between the matter particles. The gravitational forces gradually create concentrated matter and the matter structure becomes stronger. All the forces (gravitation, magnetic forces, electrical forces) are a result of collision of masses. Small masses which are gravitation particles collide with all the other masses on frequent level. These collisions create forces of affinity which lead to acceleration of the masses. Phenomenae such as atomic bomb, thermo nuclear bomb are a result of high speed collision of small or medium particles with Uranium/Hydrogen atoms. The result is partial dissipation of the atom to smaller particles which collide with the larger particle and thus travel in very high speed. The tiny particles travelling in high speed are Irradiation resulting from the reactions. The particles hit other matter, and create vibration / disruption of molecules, elevating the temperature. Regular combustion reaction is conceptually the same, with the difference that the particles are not that small and the dissipated matter is molecule and not atom. The resulting small particles are atoms, which similarly to the tiny particles created in Nuclear/Thermo nuclear reaction collide with larger particles and travel fast elevating temperature. During the dissipation of molecules, the solid matter becomes gaseous. Magnetic forces have the same nature of result of mass collisions. Electrons skip from the wire and due to wire deformation travel partially
  • 74. 74 sideways. The gravitational force affect the electrons to move mostly around the wire. Electrical forces are movement of electrons in ducts of the conductor. Since the only observation is that mass collision cause speed change of the two colliding particles can conceptually explain all the physical and chemical phenomenae we conclude that the only natures in the universe are matter and matter movement. Thermodynamics are false 3/2/2011 11:31:59 AM Thermodynamics are false. The degree of order cannot represent degree of energy. Energy is movement of matter. Out of order which is position of objects in space, we cannot gain any movement. We move to create order and this movement is energy waste which cannot be regained. ‫החשמל‬ ‫חליקי‬ ‫ע"י‬ ‫חום‬ ‫ופיזור‬ ‫גדולים‬ ‫מוליכים‬ ‫של‬ ‫אלסטיות‬ : ‫חשמל‬ 12/17/2012 11:26:49 AM ‫גוף‬ ‫או‬ ‫מנורה‬ .‫מכאנית‬ ‫לתנועה‬ ‫וגורמים‬ ‫המוליך‬ ‫על‬ ‫מתנגשים‬ ,‫החשמל‬ ‫של‬ ‫הקטנים‬ ‫החלקיקים‬ .‫ואלסטיות‬ ‫התייל‬ ‫של‬ ‫מתנועה‬ ‫אלא‬ ‫בעיקר‬ ‫מהחלקיקים‬ ‫לא‬ ‫החום‬ .‫אפשריות‬ ‫אפליקציות‬ ‫חימום‬ ‫נע‬ ‫התייל‬ ‫הקטנים‬ ‫החלקיקים‬ .‫מכאניות‬ ‫וויברציות‬ ‫קולות‬ ,‫האוויר‬ ‫טמפרטות‬ ‫בעקבות‬ ‫אלסטי‬ ‫באופןצ‬ ‫לצרכן‬ ‫האוויר‬ ‫חלקיקי‬ ‫משאר‬ ‫שתנועתי‬ ‫מהתייל‬ ‫חום‬ ‫הסעת‬ ‫מאפשרים‬ ‫וכך‬ ‫חום‬ ‫הולכת‬ ‫מעלים‬ ( ‫מנורה‬ ) . Light Speed Changes with collisions 1/15/2012 1:31:51 PM
  • 75. 75 The speed of light changes with other light beams. The light is flux of particles. Collisions change particle speed and thus light changes the speed. Flux of particles in space is from many points and thus the speed of light declines. The particles themselves are moving in different speed. The speed is seen as color in visible light. ‫נכון‬ ‫לא‬ ‫הזרם‬ ‫מושג‬ 2/21/2012 10:10:09 AM
  • 76. 76 RADIO WAVE DIFFERS SPEED NOT FREQUENCY 9/21/2012 11:12:50 AM Irradiation= PARTICLES WHICH MOVE AT SOME SPEED AT A FREQUENCY WHICH HOLDS THE SOUND INFO... SPEED=RADIO STATION. THE WIRE IS BUILT ON A CIRCULAR MAGNET WHICH DISTRACTS CERTAIN SPEED OF PARTICLES...A SINGLE SPEED IS COLLIDING TO THE WIRE AND TRAVELS TO THE TRANSISTOR, HEATING THE SILICONE, AND RESULTING Vibration OF LARGE ELECTRIC FREQUENCY =>SPEAKER!!!!!!!!! Light Speed is not uniform, WAVES WRONG... Leonid Asipov 11/7/2012 10:27:24 AM The property of color is related to the particle speed, and is function of
  • 77. 77 destructivity of the light flux. Destructivity is dissipation of speed to area. The larger the speed, the smaller the particle, the destructivity is large. Slower and larger particles are less destructive. The destructivity is barely rises with particle density because the collisions with the target are diffused to the whole area of the target. To a single unit of area (as large as a single light particle), only one light particle can cause deformation, which is read and interpreted by the biological organism as color. The strength of light is correlative to the light density, density of the particles. The frequency of the collisions with the target is correlated to the density of light. Measuring light: Measurement is a form of change in a measuring matter as a result of light flux. The changes in the matter are dependent on the matter properties (such as elasticity) as well as on the properties of the light flux. Temperature cause electrical flux and by the addition of a diode, the electrical flux is single directed and later is measured by addition of a magnet(after simple amplification). The measurement is of temperature of a measuring matter. Since matter has different heat flux properties such as different absorption of different light speeds, the temperature is a qualitative parameter to compare different light beams, and not to explain the properties of a single beam by itself. After the measurement the temperature is written as light intensity. The intensity changes according to the variation of the electricity which creates the artificial light or the nature sunlight. The sunlight is created by explosions in the sun, and thus the flux is of different density and particle speeds. Additional way to measure light is by sound measurement. The particles are hitting a small membrane which create electricity pulses. Tiny sensor (size of a light particle) will theoretically return the real single light particle amplitude (speed) and frequency (density of particles). The light "wave": wrong parameter of wrong science. The amplitude and phase are a way to track the real measurements and are not a physical phenomena. Assume we are measuring light by a small sensor with a size of a single light particle. The amplitude of a wave is correlated to the strength of the collision of the particle with the sensor. The frequency is
  • 78. 78 correlated to the density of particles. The real sensors are larger than a single light particle and thus many particles collide with sensor at a single time unit. Therefore, the amplitude is correlated to speed and density of the light. The frequency (phase of waves) is correlated to the change in intensity. Really, the waves are changes in light intensity and average particle speed. Constant light (constant density and average particle speed ) should return no waves at all. The result is a single temperature VALUE without waves. Electricity ERROR: Science fraud
  • 79. 79 11/29/2012 7:28:03 PM The - electrode of the battery should hold electrons, according to the science. However, the power is + electrode. Protons are not current. A simple connection is + to electric device and the second wire has to be - or ground. The current is from + to - . ELECTRONS ARE AT +!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! PIGMENT REFLECTION: ABSOBTION OF SPEED ... 8/5/2012 6:46:51 PM Pigment does not reflect a color : it absorbs all colors and the properties of the pigment reflect all particles to certain speed. The pigment does earn movement of light collisions. The movement elastic properties of the pigment cause most of the particles a reflection to a speed. A speed is related to visible color. ALL LIGHT IS CAUSING THE VIBRATION OF THE LEAF. ALMOST ALL LIGHT IS REFLECTED. Chemistry Periodic Table might be a falsie 5/19/2011 6:57:55 PM ‫כסטח‬ ‫הינה‬ ‫מחזורית‬ ‫טבלה‬ ‫גז‬ ‫של‬ ‫מולקולות‬ ‫בין‬ ‫שמרחק‬ ‫בהנחה‬ ‫קבוע‬ ‫גז‬ ‫נפח‬ ‫בעזרת‬ ‫נמדדה‬ ‫מולרית‬ ‫מסה‬ - ‫קבועות‬ ‫להי‬ ‫צפויים‬ ‫לא‬ ‫מרחקים‬ ‫ולכן‬ ‫מולקולות‬ ‫בין‬ ‫משיכה‬ ‫יש‬ ,‫אבל‬ ‫זהים‬ ‫ות‬ . ‫חומר‬ ‫לכל‬ ‫שונה‬ ‫חלקיקים‬ ‫מספר‬ ‫ימצאו‬ ‫גז‬ ‫של‬ ‫אחיד‬ ‫בנפח‬ ‫לכן‬ . ‫בטבלא‬ ‫מסויים‬ ‫למקום‬ ‫ומסה‬ ‫מטען‬ ‫מבחינת‬ ‫התאימו‬ ‫שלא‬ ‫לאטומים‬ ‫ברשימות‬ ‫נוספו‬ ‫נייטרונים‬ . ‫אטומי‬ ‫למספר‬ ‫בנוסף‬ ,‫המדידות‬ ‫תוצאות‬ ‫עם‬ ‫המולרית‬ ‫המסה‬ ‫מסתדרת‬ ‫הנייטרונים‬ ‫הוספת‬ ‫עם‬ ‫אחד‬ ‫בפרוטון‬ ‫תמיד‬ ‫שעולה‬ . ,‫למעשה‬ ‫מסה‬ ‫ומבחינת‬ , ‫בטבלא‬ ‫משכתוב‬ ‫אחר‬ ‫פרוטונים‬ ‫מספר‬ ‫של‬ ‫מצב‬ ‫להיות‬ ‫יכול‬ ,
  • 80. 81 ‫המספר‬ ‫באותו‬ ‫נייטרונים‬ ‫יש‬ . ‫פרוטונים‬ ‫של‬ ‫שונה‬ ‫מספר‬ ‫עם‬ ‫אך‬ ,‫מולרית‬ ‫במסה‬ ‫הזהים‬ ‫אטומים‬ ‫של‬ ‫מצבים‬ ‫גם‬ ‫ייתכנו‬ . ‫ע"י‬ ,‫מכוונת‬ ‫בצורה‬ ‫נוצרו‬ ‫המחזורים‬ .‫מדעי‬ ‫בטחון‬ ‫תחושת‬ ‫ליצירת‬ ‫נועד‬ ‫מחזורי‬ ‫סידור‬ ‫רישום‬ ‫יצירת‬ ‫להשלמת‬ ‫שנדרש‬ ‫מה‬ ‫פי‬ ‫על‬ ,‫נייטרונים‬ ‫השלמת‬ ‫עם‬ ,‫מסויים‬ ‫פרוטונים‬ ‫מספר‬ ‫על‬ ‫דומות‬ ‫תכונות‬ ‫עם‬ ‫חומרים‬ ‫העמודות‬ ‫האותן‬ ‫היו‬ ‫לא‬ ‫אחרת‬ .‫בטבלא‬ ‫המחזורים‬ . ‫שמדענים‬ ‫טענו‬ ‫ודתיים‬ .‫לדת‬ ‫מדע‬ ‫בין‬ ‫קשה‬ ‫מאבק‬ ‫היה‬ ,‫הטבלא‬ ‫כשנוצרה‬ ,‫היסטורית‬ ‫יצ‬ .‫להבנה‬ ‫ניתן‬ ‫ולא‬ ‫כאוטי‬ ‫העולם‬ ‫כי‬ ‫לכלום‬ ‫יגיעו‬ ‫לא‬ ‫ביתר‬ ‫הגיוני‬ ‫ידע‬ ‫ירת‬ , ,‫בטבלא‬ ‫שיוצר‬ ‫כפי‬ ‫נועדה‬ ‫כוחות‬ ‫של‬ ‫סודות‬ ‫לגלות‬ ‫מאמצים‬ ‫על‬ ‫לגהנום‬ ‫יגיעו‬ ‫שמדענים‬ ‫שאיימו‬ ‫דת‬ ‫אנשי‬ ‫כנגד‬ ‫כקונטרה‬ ‫גדולים‬ . ‫תחמון‬ ‫ע"י‬ ‫שהושגה‬ ,‫יתר‬ ‫הגיוניות‬ ‫של‬ ‫רוח‬ ‫עם‬ ‫עוצבה‬ ‫המחזורית‬ ‫הטבלא‬ . Periodic table wrong 4/16/2012 7:29:43 PM The periodic table mentions grams per mole Mole is incorrect due to neglection of collisions Between particles Smaller particles collide more and thus in give Volume there were expected less particles than In A mole of larger particles Grams or weight is wrong since the size causes Gravitation by flow blocking The larger is the molecule the more flow Is blocked The mass is relevant but the size is the main parameter Undense and large molecules are heavy Small and dense molecules are light Immersion or acceleration blocking of A standard
  • 81. 81 string is the precise parameter to Measure mass. Rust 4/16/2012 7:13:48 PM Rust is bulks of oxygen stuck to a metal The amount of gas particles to relative metal in gaseous State particle can be various and not precise The ordinary equation is not real there are no Distinct molecules but bulk of metal with gas particles Solids are large connected particle not Sole molecules Gas is sole particles of various size Properties of water molecule: hydrogen is light but concentrated 9/11/2011 6:42:20 PM The properties of water: gravitation of small particles. Gravitation affects differently on particles with different sizes. Small particles can be concentrated, but almost no gravitation effect because low chance to collision with gravitation particles. The effect is on weight of small particles which is very low. However, the mass might be high, and when the particles are connected with particle with large volume/mass ratio, the effect of small concentrated particles, is high molecule mass, which blocks effects of random collisions. There is high speed/mass needed to move such molecules and this effect leads to the matter to be liquid. Without the oxygen, which is large in volume/mass, hydrogen is light and hardly affected by gravitation. Hydrogen, however is concentrated and with bond to water, causes water molecule to become with large mass, and thus not easily moved. The effect of gravitation on water is through oxygen molecule, which is large volume/mass.
  • 82. 82 Hydrogen is small and concentrated: Reactivity 1/5/2012 2:36:09 PM Hydrogen is reactive. Small and light molecule would not be sufficient for any molecular damage for other molecules. The hydrogen is heavy and concentrated, but small. The small molecule has small surface area for bond with oxygen. The small bond is weak bond. The detached hydrogen is heavy and collisions with other molecules are serious structure deformators. The heaviness of hydrogen causes only two hydrogen particles to make oxygen liquid (seen as water). The gaseous state of hydrogen is due to smaller size relative to gravitation particles. water= 6H O1 2/7/2013 4:46:40 PM