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Kyril Solntsev. 1st
year accomplishments at OLIS, Inc.
Summary
 Developed new generation of OLIS instruments including absorbance, fluorescence, circular
dichroism and circularly polarized luminescence spectrometers.
 Technical support and product installations, demonstrations, and client training worldwide.
 Research studies of clients’ samples to demonstrate the applicability of OLIS equipment for
future sales.
 Worked with senior management to understand business requirements and expectations in
order to develop strategic direction for the Product Development team.
 Actively researched current and emerging trends in the industry, including all relevant areas
that might be incorporated as new technologies into future systems.
 Collaborated with sales, marketing, business development and other departments to
determine competitiveness of new technologies and to develop new products in the broad
field of optical spectroscopy.
I. Instruments installations and service visits.
Organization Instrument Purpose of visit
UGA Stopped Flow Installation. Instrument
successfully installed
(with Richard DeSa)
Montana State U DSM20 CD Installation. Instrument
successfully installed
(with Paul Boxrud)
U Nebraska Lincoln CLARiTY 1000 Installation. Instrument
successfully installed
(with Paul Boxrud)
Michigan State U
U Michigan
Xavier U
Alcorn State U
Courtesy visits. Instruments
and parts picked up and
delivered to OLIS
Loyola U Cary 14 Service visit. Instrument
repaired
Washington State U CLARiTY 17 Installation. Instrument
successfully installed
Georgia Tech Stopped Flow, protein
machine
Service visits. Instrument
repaired
Washington U Saint Louis DB620 Service visit. Instrument
tested and certified
U Kansas CLARiTY VF Installation. Instrument

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successfully installed
Kennesaw State U DM45 Service visit. Instrument
tested and certified
Weizmann Inst of Science,
Israel
CLARiTY 1000 Installation. Instrument
successfully installed
UNAM, Mexico City CLARiTY VF Installation. Instrument
successfully installed
Oregon State U CLARiTY 1000 Installation. Instrument
successfully installed
U Tennessee Knoxville DSM1000CD Service visit. Instrument
tested and certified
Virginia Commonwealth U Stopped flow Installation. Instrument
successfully installed. Parts
sent back for repair
Drexel U CLARiTY VF Installation. Instrument
successfully installed
Presbyterian College DM45 Installation. Instrument
successfully installed
Emory U Stopped flow Service visit. Instrument
repaired
Aalto Scientific, Ltd. CLARiTY CCD Installation. Instrument
successfully installed.
Software will be updated
II. Conferences and presentations.
• CLARiTY invited talks were given at ACS Georgia Section, U Kansas, ENS Paris, Weizmann
Inst, Tel Aviv U, UNAM Mexico, Moscow State U, U Tennessee Knoxville, Drexel U, Oregon
State U. This is a new form of scientific/marketing interaction with a very broad spectrum
of researchers from various research fields. Half of the talks were associated with the
CLARiTY installations, so the researchers had better feeling of the capacities of the
instruments they acquired. Another half of the talks were given at top research institutions
introducing CLARiTY technique as a breakthrough alternative to the existing methods (DW-
2/2000).
• CLARiTY poster presentation at SouthEast Enzyme Conference in Atlanta. A close
interaction with research scientists from Aalto Scientific helped them to make a decision on
CLARiTY CCD purchase.
• Participation in American Chemical Society National Meeting, Philadelphia and Biophysical
Society Meeting, Los Angeles as a vendor. Demonstration of instruments.

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III. In-house activity.
• Experimental work with on-site visitors: Federico Gonzales-Fernandez (VA Med Center,
Jackson, MS), Lynn Koplitz (Loyola U), Alex Vertegel (Clemson U), Jamie Phillips (Aalto
Scientific).
• Measurements of numerous samples from clients to demonstrate the applicability of OLIS
instruments for clients’ needs.
• QC/QA of various instruments before shipping.
IV. Research activity.
• The revised paper on Firefly luciferin (including in-house RSM1000 experiments of live
firefly bioluminescence) is submitted to J. Am. Chem. Soc. Other in-house experimental
work is acknowledged in the following publications:
• Marcero, J. R.; Piel, R. B.; Burch, J. S.; Dailey, H. A. “Rapid and sensitive quantitation of
heme in hemoglobinized cells”. BioTechniques 2016, 61, 83.
• Gonzales-Fernandez, F.; Betts-Obregon, B.; Tsin, A. T.; DeSa, R. J. “Technical brief:
Pump-probe paradigm in an integrating cavity to study photodecomposition processes”
Molecular Vision 2016, 22, 953.
• Mahmoud, M. A. “Tunable Plasmonic Neutral Density Filters and Chromatic Polarizers:
Highly Packed 2D Arrays of Plasmonic Nanoparticle on Elastomer Substrate” J. Phys.
Chem. C 2016, 120, 18249.
• The list of solved scientific/engineering problems included (but not limited to):
• Absorbance measurements of raw unfiltered red wine during fermentation and co-
pigmentation.
• Transmittance of plastic tubes used for UV-pasteurization of milk and juices.
• Monitoring of transuranium elements separation using extraction.
• Photoinduced processes in suspensions of insoluble Metal Organic Frameworks.
• Monitoring protein concentrations in turbid commercial vaccine samples.
• Study of redox processes in live cell suspensions
• Determination of hemoglobin in blood samples.
• Photoinduced transformation of chromophore in intact frog eye retina.
• Other 2016 publications.
• Carayon, C.; Ghodbane, A.; Gibot, L.; Dumur, R.; Wang, J.; Saffon, N.; Rols, M.-P.;
Solntsev, K. M.; Fery-Forgues, S. "Conjugates of Benzoxazole and GFP Chromophore with
Aggregation-Induced Enhanced Emission: Influence of the chain length on the formation
particles and the dye uptake by living cells" Small 2016, in press. Invited.

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• Olsen, S.; Baranov, M. S.; Baleeva, N. S.; Antonova, M. M.; Johnson, K. A.; Solntsev, K. M.
"pH-Sensitive fluorophores from locked GFP chromophores by a non-alternant analogue
of the photochemical meta effect." Phys. Chem. Chem. Phys. 2016, 18, 26703.
• Tang, B. Z.; Solntsev, K. M. "Themed issue on shape-responsive fluorophores." J. Mat.
Chem. C 2016, 4, 2638. Invited.
• Ghodbane, A.; Fellows, W. B.; Bright, J. R.; Ghosh, D.; Saffon, N.; Tolbert, L. M.; Fery-
Forgues, S.; Solntsev, K. M. "Effects of the Benzoxazole Group on Green Fluorescent
Protein Chromophore Crystal Structure and Solid State Photophysics." J. Mat. Chem. C
2016, 4, 2793. Invited. 2016 Journal of Materials Chemistry C Hot Paper.
• Solntsev, K. M.; Popov, A. V; Solovyeva, V. N.; Abou Al-Ainain, S.; Il'ichev, Y. V.;
Hernandez, R.; Kuzmin, M. G. "Kinetics of intra- and intermolecular excited-state proton
transfer of ω-(2-hydroxynaphthyl-1)-decanoic acid in homogeneous and micellar
solutions." Methods Appl. Fluoresc. 2016, 4, 014001. Invited. Highlights of the journal.