1. Photochemistry
Fluorescent Research
JOSE A. MORA
MARIBEL TREJO
DR. SATORU SUZUKI PH. D.
CHE490-E
CALIFORNIA BAPTIST UNIVERSITY

2. Research Intro
•Fluorescein
•Rhodamine B
•Fluorescein sodium salt
•UV-Vis
•IR Spectrum
•Gaussian

3. Preparation of Fluorescents
•Finding a good solvent was key in order to get good UV-Vis and IR data.
•Fluorescein and Rhodamine B were diluted with Methanol.
•Fluorescein sodium salt was diluted in water.
•We picked solvents that would dilute the fluorescents thoroughly and solvents
that were included in the program Gaussian in order to be able to compare our
results.

5. UV-Vis
•Three different UV-Vis spectrums were done for each fluorescent
• 1st was done at room temperature
• 2nd was done at 0 degrees Celsius
• 3rd was done at around 90 degrees Celsius
•We compared all three to try and find any changes

6. 90 degrees Celsius
Room temperature0 degrees Celsius

7. Photochemistry: Diagram

8. Discussion
BOLTZMANN DISTRIBUTION
•Overlap or vibrational functions
•Transition happens when overlap
occurs
𝑃𝑗 =
𝑒
−ℎ𝑣
𝐾 𝐵 𝑇
𝑄

9. Frank-codon principle vibrionic
transitions

10. Acknowledgement
God!
IT department
◦ Rick Hernandez
◦ Rich Davis
◦ Ana Leynes
NMS department
◦ Dr. Lisa Hernandez
◦ Dr. Tom Ferko
◦ All chem faculty
Joseph Suzuki (graphics)
James Suzuki
Spring 2015 CHE416 class
Fall 2015 CHE415 class
Maribel Trejo
Jose Mora