SlideShare ist ein Scribd-Unternehmen logo
1 von 60
Biotechnology The New Frontier
Recombinant DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The Insulin Example ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transferring Genes ,[object Object],[object Object],[object Object],[object Object]
Restriction Enzymes ,[object Object],[object Object],[object Object],[object Object]
 
Production of Recombinant DNA ,[object Object],[object Object]
 
 
Cloning a Human Gene ,[object Object],[object Object]
Forming a Recombinant Plasmid ,[object Object]
Gene Cloning ,[object Object],[object Object]
Molecular “Searching” Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Complimentarity and Hybridization ,[object Object],[object Object],[object Object],[object Object]
The Probe-Target Complex ,[object Object],[object Object],[object Object],[object Object]
Hybrid Types ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Features of Hybridization ,[object Object],[object Object],[object Object],[object Object],[object Object]
The Importance of Hybridization ,[object Object],[object Object],[object Object]
Locating the Target ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Steps in the Process ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Separation by Molecular Weight ,[object Object],[object Object],[object Object],[object Object]
Gel Electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Gel Electrophoresis
Separation of Molecules on a Gel ,[object Object],[object Object],[object Object]
Size vs. Molecular Weight ,[object Object],[object Object],[object Object],[object Object],[object Object]
Controlling for Shape ,[object Object],[object Object]
Preparation Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Determining Molecular Weight ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Use of Standards ,[object Object],[object Object],[object Object]
Staining ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transfer to Solid Support ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transfer via Electrophoresis Electrophoresis takes advantage of the molecules'  negative charge .
Capillary Blotting The molecules are transferred in a flow of buffer  from wet filter paper to dry filter paper.
Blocking ,[object Object],[object Object],[object Object],[object Object],[object Object]
Preparing the Probe ,[object Object],[object Object],[object Object],[object Object],[object Object]
Probes for Westerns ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Hybridization & Washing ,[object Object],[object Object],[object Object],[object Object]
Detecting the Probe-Target Hybrids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Southern Blot
Cloning a Gene by Hybridization :   ,[object Object],[object Object],[object Object],[object Object],[object Object]
Constructing a Genomic Library ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Genomic Libraries
Plasmid Biotechnology
Polymerase Chain Reaction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Polymerase Action ,[object Object],[object Object],[object Object]
Elements of a PCR ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Temperature Dependent Steps ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The PCR Process ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
A Polymerase Chain Reaction
DNA Fingerprinting ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
RFLP Allele Analysis
Steps in DNA Fingerprinting ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Steps in DNA Fingerprinting, Cont . ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Uses of DNA Fingerprints ,[object Object],[object Object],[object Object],[object Object]
 
Diagnosis of Inherited Disorders ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
Developing Cures for Inherited Disorders   ,[object Object],[object Object],[object Object]
Personal Identification ,[object Object],[object Object],[object Object]

Weitere ähnliche Inhalte

Was ist angesagt?

Dna sequencing powerpoint
Dna sequencing powerpointDna sequencing powerpoint
Dna sequencing powerpoint
14cummke
 
DNA sequencing - Maxam gilbert method
DNA sequencing - Maxam gilbert methodDNA sequencing - Maxam gilbert method
DNA sequencing - Maxam gilbert method
pugazhenthi6
 

Was ist angesagt? (20)

Dna sequencing ppt
Dna sequencing pptDna sequencing ppt
Dna sequencing ppt
 
DNA microarray
DNA microarrayDNA microarray
DNA microarray
 
Express sequence tags
Express sequence tagsExpress sequence tags
Express sequence tags
 
Dna sequencing
Dna sequencingDna sequencing
Dna sequencing
 
Electrophoretic mobility shift assay
Electrophoretic mobility shift assay Electrophoretic mobility shift assay
Electrophoretic mobility shift assay
 
DNA Sequencing
DNA SequencingDNA Sequencing
DNA Sequencing
 
Sanger sequencing (DNA sequencing by ENZYMATIC METHOD)
Sanger sequencing (DNA sequencing by ENZYMATIC METHOD)Sanger sequencing (DNA sequencing by ENZYMATIC METHOD)
Sanger sequencing (DNA sequencing by ENZYMATIC METHOD)
 
Probe labeling
Probe labelingProbe labeling
Probe labeling
 
Rflp technology
Rflp technologyRflp technology
Rflp technology
 
Gene library
Gene libraryGene library
Gene library
 
Pyrosequencing
PyrosequencingPyrosequencing
Pyrosequencing
 
Somatic cell hybridization
Somatic cell hybridizationSomatic cell hybridization
Somatic cell hybridization
 
Transformation and transfection
Transformation and transfection Transformation and transfection
Transformation and transfection
 
Dna sequencing powerpoint
Dna sequencing powerpointDna sequencing powerpoint
Dna sequencing powerpoint
 
DNA sequencing - Maxam gilbert method
DNA sequencing - Maxam gilbert methodDNA sequencing - Maxam gilbert method
DNA sequencing - Maxam gilbert method
 
Multiple Alignment Sequence using Clustal Omega/ Shumaila Riaz
Multiple Alignment Sequence using Clustal Omega/ Shumaila RiazMultiple Alignment Sequence using Clustal Omega/ Shumaila Riaz
Multiple Alignment Sequence using Clustal Omega/ Shumaila Riaz
 
Rapid amplification of c-DNA ends
Rapid amplification of c-DNA endsRapid amplification of c-DNA ends
Rapid amplification of c-DNA ends
 
Primers
PrimersPrimers
Primers
 
Dna sequencing
Dna sequencingDna sequencing
Dna sequencing
 
Primer design
Primer designPrimer design
Primer design
 

Andere mochten auch

Biotechnology and1 genetic engineering
Biotechnology and1 genetic engineeringBiotechnology and1 genetic engineering
Biotechnology and1 genetic engineering
mandalina landy
 
Nanotechnology 22 Feb 2017
Nanotechnology 22 Feb 2017 Nanotechnology 22 Feb 2017
Molecular probes kashmeera n.a.
Molecular probes   kashmeera n.a.Molecular probes   kashmeera n.a.
Molecular probes kashmeera n.a.
Kashmeera N.A.
 
Sds page and agarose presentation.
Sds page and agarose presentation.Sds page and agarose presentation.
Sds page and agarose presentation.
khrystallramos
 
Nanotechnology And Its Applications
Nanotechnology And Its ApplicationsNanotechnology And Its Applications
Nanotechnology And Its Applications
mandykhera
 

Andere mochten auch (20)

General Overview of INBT
General Overview of INBTGeneral Overview of INBT
General Overview of INBT
 
NanoCultures: On Speculation, Bioethics and Paranoia (2008)
NanoCultures: On Speculation, Bioethics and Paranoia (2008)NanoCultures: On Speculation, Bioethics and Paranoia (2008)
NanoCultures: On Speculation, Bioethics and Paranoia (2008)
 
Nanotechnology
NanotechnologyNanotechnology
Nanotechnology
 
B.sc. (micro) i em unit 4.4 culturing & isolating
B.sc. (micro) i em unit 4.4 culturing & isolatingB.sc. (micro) i em unit 4.4 culturing & isolating
B.sc. (micro) i em unit 4.4 culturing & isolating
 
Isolation in Tennyson
Isolation in TennysonIsolation in Tennyson
Isolation in Tennyson
 
Biotechnology and1 genetic engineering
Biotechnology and1 genetic engineeringBiotechnology and1 genetic engineering
Biotechnology and1 genetic engineering
 
Ap Bio Ch 13 Power Point
Ap Bio Ch 13 Power PointAp Bio Ch 13 Power Point
Ap Bio Ch 13 Power Point
 
RNA extraction and gel electrophoresis
RNA extraction and gel electrophoresisRNA extraction and gel electrophoresis
RNA extraction and gel electrophoresis
 
cell Viability princes
cell Viability  princescell Viability  princes
cell Viability princes
 
Carbon nano tube (cnts) by a.wahab
Carbon nano tube (cnts) by a.wahabCarbon nano tube (cnts) by a.wahab
Carbon nano tube (cnts) by a.wahab
 
Nanotechnology 22 Feb 2017
Nanotechnology 22 Feb 2017 Nanotechnology 22 Feb 2017
Nanotechnology 22 Feb 2017
 
Molecular probes kashmeera n.a.
Molecular probes   kashmeera n.a.Molecular probes   kashmeera n.a.
Molecular probes kashmeera n.a.
 
Biotechnology
BiotechnologyBiotechnology
Biotechnology
 
Hematology analysor and its working
Hematology analysor and its workingHematology analysor and its working
Hematology analysor and its working
 
2 d gel electrophoresis
2 d gel electrophoresis2 d gel electrophoresis
2 d gel electrophoresis
 
Sds page and agarose presentation.
Sds page and agarose presentation.Sds page and agarose presentation.
Sds page and agarose presentation.
 
Plegab
PlegabPlegab
Plegab
 
An Overview Of Nanotechnology In Medicine
An Overview Of  Nanotechnology In MedicineAn Overview Of  Nanotechnology In Medicine
An Overview Of Nanotechnology In Medicine
 
Nanotechnology baseddrugdelivery.ppt
Nanotechnology baseddrugdelivery.pptNanotechnology baseddrugdelivery.ppt
Nanotechnology baseddrugdelivery.ppt
 
Nanotechnology And Its Applications
Nanotechnology And Its ApplicationsNanotechnology And Its Applications
Nanotechnology And Its Applications
 

Ähnlich wie Biotechnology

Southern-blotting-and-Western-blotting.pptx
Southern-blotting-and-Western-blotting.pptxSouthern-blotting-and-Western-blotting.pptx
Southern-blotting-and-Western-blotting.pptx
KhushiDuttVatsa
 
Getitics Slides #1 - Modified
Getitics Slides #1 - Modified Getitics Slides #1 - Modified
Getitics Slides #1 - Modified
Ahmad B. Younes
 
Biotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blotsBiotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blots
Nikolay Vyahhi
 
Biotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blotsBiotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blots
Nikolay Vyahhi
 
Detection and Measurement of Genetic Variation.pptx
Detection and Measurement of Genetic Variation.pptxDetection and Measurement of Genetic Variation.pptx
Detection and Measurement of Genetic Variation.pptx
alizain9604
 

Ähnlich wie Biotechnology (20)

Techniques Used in Recombinant DNA Technology
Techniques Used in Recombinant DNA TechnologyTechniques Used in Recombinant DNA Technology
Techniques Used in Recombinant DNA Technology
 
blooting image.pptx
blooting image.pptxblooting image.pptx
blooting image.pptx
 
IDENTIFICATION OF PROTEIN BINDING SITE.docx
IDENTIFICATION OF PROTEIN BINDING SITE.docxIDENTIFICATION OF PROTEIN BINDING SITE.docx
IDENTIFICATION OF PROTEIN BINDING SITE.docx
 
Blotting type and uses
Blotting type and usesBlotting type and uses
Blotting type and uses
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Northern, southern and western blotting
Northern, southern and western blottingNorthern, southern and western blotting
Northern, southern and western blotting
 
2017 summer high school textbook (molecular biology and neuroscience)
2017 summer high school textbook (molecular biology and neuroscience)2017 summer high school textbook (molecular biology and neuroscience)
2017 summer high school textbook (molecular biology and neuroscience)
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Restriction Digestion and its Applications
Restriction Digestion and its ApplicationsRestriction Digestion and its Applications
Restriction Digestion and its Applications
 
unit 4 immunoblotting technique complete.pptx
unit 4 immunoblotting technique complete.pptxunit 4 immunoblotting technique complete.pptx
unit 4 immunoblotting technique complete.pptx
 
Southern-blotting-and-Western-blotting.pptx
Southern-blotting-and-Western-blotting.pptxSouthern-blotting-and-Western-blotting.pptx
Southern-blotting-and-Western-blotting.pptx
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Recombinant DNA Technology
Recombinant DNA TechnologyRecombinant DNA Technology
Recombinant DNA Technology
 
Getitics Slides #1 - Modified
Getitics Slides #1 - Modified Getitics Slides #1 - Modified
Getitics Slides #1 - Modified
 
Biotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blotsBiotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blots
 
Biotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blotsBiotech 2011-06-electrophoresis-blots
Biotech 2011-06-electrophoresis-blots
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Library screening
Library screeningLibrary screening
Library screening
 
Recombinant DNA Technology
Recombinant DNA TechnologyRecombinant DNA Technology
Recombinant DNA Technology
 
Detection and Measurement of Genetic Variation.pptx
Detection and Measurement of Genetic Variation.pptxDetection and Measurement of Genetic Variation.pptx
Detection and Measurement of Genetic Variation.pptx
 

Mehr von Jolie Yu

Classification
ClassificationClassification
Classification
Jolie Yu
 
Bacteria & Viruses
Bacteria & VirusesBacteria & Viruses
Bacteria & Viruses
Jolie Yu
 
Evolution1
Evolution1Evolution1
Evolution1
Jolie Yu
 
Evolution2
Evolution2Evolution2
Evolution2
Jolie Yu
 
Evolution3
Evolution3Evolution3
Evolution3
Jolie Yu
 
Population Genetics
Population GeneticsPopulation Genetics
Population Genetics
Jolie Yu
 
Eukaryotic Gene Regulation
Eukaryotic Gene RegulationEukaryotic Gene Regulation
Eukaryotic Gene Regulation
Jolie Yu
 
Prokaryotic Gene Regulation
Prokaryotic Gene RegulationProkaryotic Gene Regulation
Prokaryotic Gene Regulation
Jolie Yu
 
Molecular Genetics Part II
Molecular Genetics Part IIMolecular Genetics Part II
Molecular Genetics Part II
Jolie Yu
 
Molecular Genetics
Molecular GeneticsMolecular Genetics
Molecular Genetics
Jolie Yu
 
Genetic Linkage
Genetic LinkageGenetic Linkage
Genetic Linkage
Jolie Yu
 
Chi Square
Chi SquareChi Square
Chi Square
Jolie Yu
 
Cell Reproduction
Cell ReproductionCell Reproduction
Cell Reproduction
Jolie Yu
 
Photosynthesis
PhotosynthesisPhotosynthesis
Photosynthesis
Jolie Yu
 
Cellular Energy pt.2
Cellular Energy pt.2Cellular Energy pt.2
Cellular Energy pt.2
Jolie Yu
 
Cellular Energy pt.1
Cellular Energy pt.1Cellular Energy pt.1
Cellular Energy pt.1
Jolie Yu
 

Mehr von Jolie Yu (20)

Protists
ProtistsProtists
Protists
 
Fungi
FungiFungi
Fungi
 
Classification
ClassificationClassification
Classification
 
Bacteria & Viruses
Bacteria & VirusesBacteria & Viruses
Bacteria & Viruses
 
Evolution1
Evolution1Evolution1
Evolution1
 
Evolution2
Evolution2Evolution2
Evolution2
 
Evolution3
Evolution3Evolution3
Evolution3
 
Population Genetics
Population GeneticsPopulation Genetics
Population Genetics
 
Eukaryotic Gene Regulation
Eukaryotic Gene RegulationEukaryotic Gene Regulation
Eukaryotic Gene Regulation
 
Prokaryotic Gene Regulation
Prokaryotic Gene RegulationProkaryotic Gene Regulation
Prokaryotic Gene Regulation
 
Molecular Genetics Part II
Molecular Genetics Part IIMolecular Genetics Part II
Molecular Genetics Part II
 
Molecular Genetics
Molecular GeneticsMolecular Genetics
Molecular Genetics
 
Mendel
MendelMendel
Mendel
 
Genetic Linkage
Genetic LinkageGenetic Linkage
Genetic Linkage
 
Chi Square
Chi SquareChi Square
Chi Square
 
Cell Reproduction
Cell ReproductionCell Reproduction
Cell Reproduction
 
Photosynthesis
PhotosynthesisPhotosynthesis
Photosynthesis
 
Cellular Energy pt.2
Cellular Energy pt.2Cellular Energy pt.2
Cellular Energy pt.2
 
Cellular Energy pt.1
Cellular Energy pt.1Cellular Energy pt.1
Cellular Energy pt.1
 
Membranes pt. 2
Membranes pt. 2Membranes pt. 2
Membranes pt. 2
 

Kürzlich hochgeladen

Salient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functionsSalient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functions
KarakKing
 
The basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptxThe basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptx
heathfieldcps1
 

Kürzlich hochgeladen (20)

Salient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functionsSalient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functions
 
How to setup Pycharm environment for Odoo 17.pptx
How to setup Pycharm environment for Odoo 17.pptxHow to setup Pycharm environment for Odoo 17.pptx
How to setup Pycharm environment for Odoo 17.pptx
 
HMCS Vancouver Pre-Deployment Brief - May 2024 (Web Version).pptx
HMCS Vancouver Pre-Deployment Brief - May 2024 (Web Version).pptxHMCS Vancouver Pre-Deployment Brief - May 2024 (Web Version).pptx
HMCS Vancouver Pre-Deployment Brief - May 2024 (Web Version).pptx
 
FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024
 
The basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptxThe basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptx
 
Micro-Scholarship, What it is, How can it help me.pdf
Micro-Scholarship, What it is, How can it help me.pdfMicro-Scholarship, What it is, How can it help me.pdf
Micro-Scholarship, What it is, How can it help me.pdf
 
Holdier Curriculum Vitae (April 2024).pdf
Holdier Curriculum Vitae (April 2024).pdfHoldier Curriculum Vitae (April 2024).pdf
Holdier Curriculum Vitae (April 2024).pdf
 
On National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan FellowsOn National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan Fellows
 
ICT Role in 21st Century Education & its Challenges.pptx
ICT Role in 21st Century Education & its Challenges.pptxICT Role in 21st Century Education & its Challenges.pptx
ICT Role in 21st Century Education & its Challenges.pptx
 
Interdisciplinary_Insights_Data_Collection_Methods.pptx
Interdisciplinary_Insights_Data_Collection_Methods.pptxInterdisciplinary_Insights_Data_Collection_Methods.pptx
Interdisciplinary_Insights_Data_Collection_Methods.pptx
 
Basic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptxBasic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptx
 
General Principles of Intellectual Property: Concepts of Intellectual Proper...
General Principles of Intellectual Property: Concepts of Intellectual  Proper...General Principles of Intellectual Property: Concepts of Intellectual  Proper...
General Principles of Intellectual Property: Concepts of Intellectual Proper...
 
Sociology 101 Demonstration of Learning Exhibit
Sociology 101 Demonstration of Learning ExhibitSociology 101 Demonstration of Learning Exhibit
Sociology 101 Demonstration of Learning Exhibit
 
Sensory_Experience_and_Emotional_Resonance_in_Gabriel_Okaras_The_Piano_and_Th...
Sensory_Experience_and_Emotional_Resonance_in_Gabriel_Okaras_The_Piano_and_Th...Sensory_Experience_and_Emotional_Resonance_in_Gabriel_Okaras_The_Piano_and_Th...
Sensory_Experience_and_Emotional_Resonance_in_Gabriel_Okaras_The_Piano_and_Th...
 
This PowerPoint helps students to consider the concept of infinity.
This PowerPoint helps students to consider the concept of infinity.This PowerPoint helps students to consider the concept of infinity.
This PowerPoint helps students to consider the concept of infinity.
 
Jamworks pilot and AI at Jisc (20/03/2024)
Jamworks pilot and AI at Jisc (20/03/2024)Jamworks pilot and AI at Jisc (20/03/2024)
Jamworks pilot and AI at Jisc (20/03/2024)
 
SKILL OF INTRODUCING THE LESSON MICRO SKILLS.pptx
SKILL OF INTRODUCING THE LESSON MICRO SKILLS.pptxSKILL OF INTRODUCING THE LESSON MICRO SKILLS.pptx
SKILL OF INTRODUCING THE LESSON MICRO SKILLS.pptx
 
Spatium Project Simulation student brief
Spatium Project Simulation student briefSpatium Project Simulation student brief
Spatium Project Simulation student brief
 
UGC NET Paper 1 Mathematical Reasoning & Aptitude.pdf
UGC NET Paper 1 Mathematical Reasoning & Aptitude.pdfUGC NET Paper 1 Mathematical Reasoning & Aptitude.pdf
UGC NET Paper 1 Mathematical Reasoning & Aptitude.pdf
 
ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.
 

Biotechnology