1. Glyphosate—no chemical does more on a widespread basis to promote disease:
1.Inhibits cyp450 detoxification: Anything that blocks cyp450 by definition promotes xenobiotic accumulation and
adverse drug effects
2.Genotoxic: Causes DNA damage in human cells even when massively diluted
3.Mitochondriopathic: In the combination of Monsanto’s Roundup®, causes mitochondrial dysfunction per several
in vitro studies, linked to nearly all diseases
4.Excellent tissue penetration: The added solvents, surfactants, and so-called inert chemicals help promote entry
into cells
5.Carcinogenic: Activation of estrogen receptors stimulates growth of cancer cells in vitro; would be expected to
promote growth of other cancer cells.
6.Estrogenic: Activation of estrogen receptors stimulates growth of cancer cells in vitro; would be expected to
promote growth of other cancer cells, as well as promote autoimmunity.
7.Synergistic with GMO-promoted inflammation: Commonly applied to GMO corn which has been shown to
promote proliferation of GammaDelta Tcells which promote allergy and autoimmunity, both of which would be
exacerbated by 1) inhibition of detoxification, 2) genotoxicity, 3) mitochondrial dysfunction, and 4) estrogenic
effects.
8.Unsurpassed political protection: Because of industry influence on politicians who have 1) no scientific training,
and 2) receive pay-off money from the chemical industry, glyphosate is used in high doses internationally without
concern for long-term effects, even when applied to fields, forests, water supplies and other ecosystems.
9.Widespread exposure via air and water: More than 75% of air and water samples in Mississippi USA are
contaminated with glyphosate; that far exceeds the social acceptance of heroin, crack cocaine, tobacco use and
second-hand smoke combined. No other chemical achieves such massive population-wide use.
10.Unsurpassed distribution to the population, routinely found in the food supply, especially soy: "GM-soy
contains high residues of glyphosate and AMPA…" Food Chemistry 2014 Jun
2. Glyphosate inhibition of Cyp450
detoxification
•Expected effects of anything that impairs cyp450
detoxification:
– Accumulation of toxins; Increased adverse drug effects;
Accumulation of self-same toxin
6. Mitochondrial toxicity of
glyphostate/Roundup®
Toxicol In Vitro. 2013 Feb;27(1):191-7. doi: 10.1016/j.tiv.2012.09.021. Epub 2012 Oct 23.
Mixtures of glyphosate and surfactant TN20 accelerate cell death via mitochondrial damage-
induced apoptosis and necrosis.
Kim YH1
, Hong JR, Gil HW, Song HY, Hong SY.
Author information
Abstract
Glyphosate, a common herbicide, is not toxic under normal exposure circumstances. However,
this chemical, when combined with a surfactant, is cytotoxic. In this study, the mechanism of the
additive effect of glyphosate and TN-20, a common surfactant in glyphosate herbicides, was
investigated. After exposure of rat H9c2 cells to glyphosate and TN-20 mixtures, following assays
were performed: flow cytometry to determine the proportion of cells that underwent apoptosis
and necrosis; western blotting to determine expression of mitochondrial proteins (Bcl-2 and
Bax); immunological methods to evaluate translocation of cytochrome C; luminometric
measurements to determine activity of caspases 3/7 and 9; and tetramethyl rhodamine methyl
ester assay to measure mitochondrial membrane potentials. Bcl-1 intensity decreased while Bax
intensity increased with exposure to increasing TN-20 and/or glyphosate concentrations.
Caspase activity increased and mitochondrial membrane potential decreased only when the cells
were exposed to a mixture of both TN-20 and glyphosate, but not after exposure to either one of
these compounds. The results support the possibility that mixtures of glyphosate and TN-20
aggravate mitochondrial damage and induce apoptosis and necrosis. Throughout this process,
TN-20 seems to disrupt the integrity of the cellular barrier to glyphosate uptake,
promoting glyphosate-mediated toxicity.
www.ncbi.nlm.nih.gov/pubmed
7. Mitochondrial toxicity of
glyphostate/Roundup®
Environ Toxicol Pharmacol. 2012 Sep;34(2):144-53. doi: 10.1016/j.etap.2012.02.010.
Epub 2012 Mar 15.
A step further toward glyphosate-induced epidermal cell death: involvement
of mitochondrial and oxidative mechanisms.
Heu C1
, Elie-Caille C, Mougey V, Launay S, Nicod L.
Author information
Abstract
A deregulation of programmed cell death mechanisms in human epidermis leads to
skin pathologies. We previously showed that glyphosate, an extensively used
herbicide, provoked cytotoxic effects on cultured human keratinocytes, affecting
their antioxidant capacities and impairing morphological and functional cell
characteristics. The aim of the present study, carried out on the human epidermal
cell line HaCaT, was to examine the part of apoptosis plays in the cytotoxic effects
of glyphosate and the intracellular mechanisms involved in the apoptotic events.
We have conducted different incubation periods to reveal the specific events
in glyphosate-induced cell death. We observed an increase in the number of early
apoptotic cells at a low cytotoxicity level (15%), and then, a decrease, in favor of
late apoptotic and necrotic cell rates for more severe cytotoxicity conditions. At the
same time, we showed that the glyphosate-induced mitochondrial membrane
potential disruption could be a cause of apoptosis in keratinocyte cultures.
www.ncbi.nlm.nih.gov/pubmed
8. Mitochondrial toxicity of
glyphostate/Roundup®
Chemosphere. 2005 Dec;61(8):1115-22. Epub 2005 Apr 26.
Comparative effects of the Roundup and glyphosate on mitochondrial oxidative phosphorylation.
Peixoto F.
Author information
Abstract
The potential toxicity of the herbicide Roundup and its fundamental substance (glyphosate) was tested in
bioenergetic functions of isolated rat liver mitochondria. Roundup stimulates succinate-supported respiration
twice, with simultaneous collapse of transmembrane electrical potential, while glyphosate used in the same
concentrations does not induce any significant effect. Additionally, Roundup depresses state 3 respiration by
about 40%, at 15 mM, whereas uncoupled respiration in the presence of FCCP is depressed by about 50%.
Depression of uncoupled respiratory activity is mediated through partial inhibition ofmitochondrial complexes II
and III, but not of complex IV. The phosphorylative system was affected by both a direct and an indirect effect on
the F0F1 ATPase activity. The addition of uncoupled concentrations of Roundup to Ca2+-
loaded mitochondriatreated with Ruthenium Red resulted in non-specific membrane permeabilization, as
evidenced by mitochondrial swelling in isosmotic sucrose medium. Therefore, the uncoupling of oxidative
phosphorylation is also related to the non-specific membrane permeabilization induced
by Roundup. Glyphosate alone does not show any relevant effect on the mitochondrial bioenergetics, in
opposition to Roundup formulation products. The differences in the toxicity observed could be either attributed to
some products of Roundup or to a synergic effect of glyphosate and formulation products. Bearing in mind
that mitochondria is provided with a variety of bioenergetic functions mandatory for the regulation of intracellular
aerobic energy production and electrolyte homeostasis, these results question the safety of Roundup on animal
health.
www.ncbi.nlm.nih.gov/pubmed
9. Tissue penetration
•Glyphosate is almost always combined with other
chemicals—these are commonly mislabeled “inert”
chemicals
– These other chemicals increase toxicity of glyphosate and
also have their own toxic effects
•Solvents and surfactants basically dissolve either
glyphosate into aqueous solutions and/or damage cell
membranes to promote chemical entry
10. Carcinogen: substance that
promotes cancer, generally via 1)
mutation and/or 2) proliferation
•Mutation = DNA damage
•Proliferation = such as via estrogen receptors
11. Carcinogen: substance that
promotes cancer, generally via 1)
mutation and/or 2) proliferation
•Mutation = DNA damage
•Proliferation = such as via estrogen receptors
12. GMO corn shown to double the
population of gamma-delta T-cells,
which promote inflammation,
allergy and autoimmunity
13. GMO-altered foods: Increased allergenicity, toxins,
changes in gut microbes, gut permeability,
cytokine levels, and immunocyte populations…
•MON810 corn causes increased IL-6 and gd-Tcells:
– GMO MON810 maize induced increases of serum IL-
6, IL-13, IL-12p70, and MIP-1beta and gamma-delta-
Tcells of mice.
• We all know that IL-6 = inflammation and
generally more CRP/IR/CVD/AzD.
– “One of the more recurrent alterations in lymphocyte
phenotypes observed in this study was an increase
in the TCRγδ+ population. ... Higher numbers of γδT
cells have been observed in humans with asthma, in
IELs of children with untreated food allergy, and in
the duodenum of children with juvenile idiopathic
arthritis or connective tissue disease with
gastrointestinal symptoms.” Finamore et al. J Agric
Food Chem. 2008 Dec
– Per review in Feb 2013 and previous work dating
back decades, we know that gd-Tcells promote
inflammation and autoimmunity! Su et al. Clin Dev
Immunol. 2013 Feb
14. GMO-altered foods: Increased allergenicity,
toxins, changes in gut microbes, gut
permeability, cytokine levels, and immunocyte
populations…
•MON810 corn causes increased IL-6 and gd-
Tcells:
– Finamore et al. J Agric Food Chem. 2008 Dec
“As a subset of T cells that bridge innate and
adaptive immunity, γδ T cells may display
different functions similar to those of CD4+ T-cell
subsets such as CTLs, Th1/Th2 cells, Tregs, Th17
cells, and APCs depending on specific
microenvironment. They definitely play
important roles in the development of
autoimmune diseases such as RA and SLE
through their antigen-presenting capacity, release
of proinflammatory cytokines, immunomodulatory
properties, interaction with CD4+CD25+Tregs, and
promotion of antibody production by providing B-
cell help.” Su et al. Clin Dev Immunol. 2013 Feb
15. Unreasonable political protection
Paul Craig Roberts PhD is a
former Assistant Secretary
of the US Treasury and
Associate Editor of the Wall
Street Journal.
counterpunch.org/2013/02/26/one-nation-under-monsanto/
19. Glyphosate found in more than
75% of air and water in Mississippi
USA
•Pesticides in Mississippi air and rain: a comparison between
1995 and 2007. Environmental Toxicology and Chemistry 2014
– "Atrazine, metolachlor, and propanil were detected in ≥50% of
the air and rain samples in both years. Glyphosate and its
degradation product, aminomethyl-phosphonic acid
(AMPA), were detected in ≥75% of air and rain samples
in 2007 but were not measured in 1995. … Total herbicide flux
in 2007 was slightly greater than in 1995 and was dominated by
glyphosate. Malathion, methyl parathion, and degradation
products made up most of the 2007 nonherbicide flux.“
20. Glyphosate in food, especially GM
(genetically manipulated) foods, like wheat
and soy ers.usda.gov/data-products/adoption-of-genetically-engineered-crops-in-the-us/
21. Glyphosate in food, especially GM
(genetically manipulated) foods,
like wheat and soy
22. Glyphosate in food, especially GM
(genetically manipulated) foods,
like wheat and soy
ewg.org/agmag/2012/10/americans-eat-their-weight-genetically-engineered-
26. Other concerns (?)
• Damage to amphibians
• Killing bee populations
• Reproductive toxicity to many species
• Political misuse against domestic
populations in the “War on Drugs”
• Brain diseases, behavior problems
27. "Tensions have
been rising
between the
Colombian
government and the
agricultural sector
since the
regulations
imposed by the
U.S.-Colombia Free
Trade Agreement,
which went into
effect in May 2012.
The agreement
aimed to promote
economic growth in
both nations by
phasing out tariffs
28. The “War on Drugs” ends up
poisoning poor farms with glyphosate,
killing innocent crops and injuring
animals and farmers—men, women,
children.
topdocumentaryfilms.com/america-
backyard-colombia/
29. How did Colombians overturn laws
mandating the destruction of their grains,
their mining, (ie, their food and ways of
living)?
•Organization, protests and strikes in many cities
•648 arrests of protesters, 262 detentions (overturned)
30. Other concerns (?)
• Damage to amphibians
• Killing bee populations
• Reproductive toxicity to many species
• Political misuse against domestic populations in the “War
on Drugs”
• If these topics are completely new to you, then you should
consider examining the following:
– The size and weight of the rock that you are living under
– How far you have your head buried under the sand
– Media censorship that keeps you ignorant about issues that
critically affect your life
– Why you have placed blinders on yourself, insulating yourself
from reality, despite the consequences
• All of the information in this presentation is readily and publically available
31. Glyphosate research
• How to learn about glyphosate:
1. Open your eyes and activate your mind,
2. Surf the internet; if your friends aren’t talking about this
issue, then your friends/colleagues are ignorant and you
should get new contacts in your life who are actually
engaged in the world,
3. Go to the US Library of Medicine website and type the
word “glyphosate”—you can also type any disease
condition along with that:
http://www.ncbi.nlm.nih.gov/pubmed/?term=glyphosate
32.
33.
34.
35.
36.
37.
38.
39. Glyphosate research
• How to learn about glyphosate:
1. Open your eyes and activate your mind,
2. Search the internet; if your friends aren’t talking about
this issue, then your friends/colleagues are ignorant and
you should get new contacts in your life who are actually
engaged in the world,
3. Go to the US Library of Medicine website and type the
word “glyphosate”—you can also type any disease
condition along with that:
http://www.ncbi.nlm.nih.gov/pubmed/?term=glyphosate
40. Other concerns (?)
• Damage to amphibians
• Killing bee populations
• Reproductive toxicity to many species
• Political misuse against domestic populations in the “War
on Drugs”
• If these topics are completely new to you, then you should
consider examining the following:
– The size and weight of the rock that you are living under
– How far you have your head buried under the sand
– Media censorship that keeps you ignorant about issues that
critically affect your life
– Why you have placed blinders on yourself, insulating yourself
from reality, despite the consequences
• All of the information in this presentation is readily and publically available
41. Glyphosate—no chemical does more on a widespread basis to promote disease:
1.Inhibits cyp450 detoxification: Anything that blocks cyp450 by definition promotes xenobiotic accumulation and
adverse drug effects
2.Genotoxic: Causes DNA damage in human cells even when massively diluted
3.Mitochondriopathic: In the combination of Monsanto’s Roundup®, causes mitochondrial dysfunction per several
in vitro studies, linked to nearly all diseases
4.Excellent tissue penetration: The added solvents, surfactants, and so-called inert chemicals help promote entry
into cells
5.Carcinogenic: Activation of estrogen receptors stimulates growth of cancer cells in vitro; would be expected to
promote growth of other cancer cells.
6.Estrogenic: Activation of estrogen receptors stimulates growth of cancer cells in vitro; would be expected to
promote growth of other cancer cells, as well as promote autoimmunity.
7.Synergistic with GMO-promoted inflammation: Commonly applied to GMO corn which has been shown to
promote proliferation of GammaDelta Tcells which promote allergy and autoimmunity, both of which would be
exacerbated by 1) inhibition of detoxification, 2) genotoxicity, 3) mitochondrial dysfunction, and 4) estrogenic
effects.
8.Unsurpassed political protection: Because of industry influence on politicians who have 1) no scientific training,
and 2) receive pay-off money from the chemical industry, glyphosate is used in high doses internationally without
concern for long-term effects, even when applied to fields, forests, water supplies and other ecosystems.
9.Widespread exposure via air and water: More than 75% of air and water samples in Mississippi USA are
contaminated with glyphosate; that far exceeds the social acceptance of heroin, crack cocaine, tobacco use and
second-hand smoke combined. No other chemical achieves such massive population-wide use.
10.Unsurpassed distribution to the population, routinely found in the food supply, especially soy: "GM-soy
contains high residues of glyphosate and AMPA…" Food Chemistry 2014 Jun
