Presentation from OIS@ASCRS 2017 Chris Geddes, MD, Chief Biomedical/Plasmonics Consultant

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The LacriPen™ – A Handheld Portable Ophthalmic
Osmometer and Diagnostic Instrument
…a portable laboratory in the palm of your hand
Presented by:
Dr Chris D. Geddes, Ph.D., FRSC
Professor, University of Maryland (UMBC) for
LacriScience LLC, Paul T. Gavaris MD
Managing Member

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LacriPen™ - Point of Care Diagnostics: A Reality
Osmolality – Previously debuted Goal ± 2 mOsms/L – 1 sec
MMP-9 – Analytical readout 1 – 200 ng/ ml; ± 5 ng/ml
Developed Universal Platform MMP-9; Adenovirus; MRSA
Started Clinical Testing 2 Locations – IRB
Continued Bench Testing New data handling
Talk Overview and Update since OIS Chicago

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Surface Plasmon Resonance
“Precision Engineering”
LacriPen™
From bench top…………… to the eye
Rapid Measurements < 1 sec
Disposable – one time use Sensor
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5.1
5.2
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5.9
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280 300 320 340
950-855sol/950–
855air
Osmolarity, mOsms/L
Two Wavelength Ratiometric Measurements
Good Sensor Prism to Prism Reproducibility Improved Manufacturing Processes
Excellent Linear Pen Responses Salt Solutions, Contrived Tears
Pen to Pen Reproducibility Developing correction factors
We use a Wavelength Ratiometric approach
• Divide dual channel signals
• Corrects for Prism-to-Prism variations
• Corrects for LED intensity differences
• Corrects for manufacturing differences

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MMP-9
Specifically detect MMP-9 from 1 – 200 ng/ml
Quantitation of MMP-9 – Monitor Treatment
Precision  5 ng/ml – No other device today
No Anesthesia; No Scraping of the Eyelid
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Point of CareLacriPen™
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0
20
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60
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SPRDeltaPixel
Shift
MMP-9 ng / ml
Representative Data from Testing
(855 channel)
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SPRDeltaPixel
Shift
MMP-9 ng / ml
Enlarged Region

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Unique Proprietary Scaffold –
Universal for other Affinity Interactions
Antibody (DNA or RNA)
Antigen of Interest
Unique Universal Scaffold – Reduced Cost / Development Time
Tunable Dynamic Sensing Range – Binding Site / Surface Capacity
Disposable Sensor Prism
Disposable Sensor Prism
Gold Film
CM-Dextran Coating
Antibody for analyte, MMP-9, MRSA etc
Surface Blocking,
NHS-ester coupling
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LacriPen™ Diagnostics
Select Diagnostic Results
#1
#2
#3
#4
#5
For Future Diagnostics – Bluetooth input
MMP-9
Adenovirus
Tryptase
Gm-Neg Bact
MRSA
ng/ml
ng/ml
Pos/Neg
Pos/Neg
Pos/Neg

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Update on Clinical Testing
Dual Path – Osmolarity and Smart Diagnostics
IRB Approved in February 2017 for 15 patient study
Testing on April 19th 2017 Completed
Clinical Testing at Virginia Eye Consultants

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The LacriPen™ is easy to Use and Rapid
Low Cost of Pen  $1500
Diagnostic Intelligent Sensors  $7  $9
Instantaneous 1 second Readout
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Thank You
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Changing the Way we Think
about and Use Ophthalmic Diagnostics Today
The Lacripen™