1. Luminate® for the
Treatment of Retinal Diseases
Ophthalmology Innovation Summit
November 12, 2015
Vicken Karageozian, MD
Chief Technical Officer
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
2. Allegro Ophthalmics: Integrin Peptide
Therapy for Vitreo-Retinal Eye Diseases
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Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
Mid-stage biotechnology company
In multiple Phase 2b US Studies in DME &
PVD
Phase 2b VMT – met primary endpoint
Differentiated mechanisms of action with
unique benefits
Anti-integrin vs. anti-VEGF
3+ months durability in DME and wAMD
Robust vitreolysis
Strong safety and efficacy profile with
>400 human injections to date
Collaboration with strategic partners
3. Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
• 1st in class anti-integrin peptide
• Potency comparable to anti-VEGF in mono-therapy
• 3+ months durability off-treatment
• Robust vitreolysis with an excellent safety profile
Anti-angiogenesis Vitreolysis
DME
US Phase 2b
Data Q3 16
Wet AMD
US Phase 2
Start mid 2016
VMT
US Phase 2b
Met 1º Endpoint
PVD for NPDR
US Phase 2b
Data Q3 16
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5. Key Market Differentiators for Luminate®
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
5
Jetrea
Safety
Serious neurotoxicity
Retinal tears and
detachment
Very well-tolerated; no
significant safety issues
Efficacy
26% efficacy rate in phase
3 studies
65% efficacy rate in latest
phase 2 study
No competition in PVD
Vitreolysis: VMT and PVD
6. At least 100 subjects in 4 arms with VMT with or without MH
Double masked, placebo-controlled, randomized, multi-center study in the
US and Eastern Europe
2.0, 2.5 and 3.2mg Luminate® vs BSS
3-month study with evaluation after 1, 2 and 3 monthly injections
Primary endpoint: resolution of VMT by OCT by end of study (day 90)
Enrollment and endpoint determinations made by Duke Reading Center
Phase IIb VMT Study: Design
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
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45 day washout or
treatment naïve
Baseline 1 2 3
Months
7. Phase IIb VMT Study: Safety
106 subjects enrolled from US and E. European sites
Excellent Safety Profile:
No study subjects in any of the three groups
experienced:
Significant intraocular inflammation
Cataract changes
IOP changes
Retinal tears
Retinal detachments
Neurotoxicity - afferent pupillary defects
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
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8. Phase IIb VMT Study: Topline Efficacy
8Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
Baseline Day 90
9. Phase IIb VMT Study: Statistical Analysis
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
• Phase 2 study met its primary endpoint with 65%
efficacy rate of VMT release vs 10% for BSS control
• 3.2mg Luminate® vs BSS
• This result was statistically significant at a 95%
confidence level at p=0.0129
• Data suggests pathway to phase 3
• Data confirms Luminate® robust vitreolytic activity
in human subjects
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10. US Phase IIb PVD (PACIFIC) Study
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100 subjects with non-proliferative DR without PVD
Up to 3 intravitreal monthly injections - 1.0, 2.0, and 3.0 mg
Luminate vs BSS
PVD induction in diabetics closely associated with protection
against PDR progression
New indication with novel endpoint
Duke reading center for endpoint grading
First patient enrolled 09/15
Top line data Q3 2016
90 day washout
Baseline 1 2 3
Months
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
12. Key Market Differentiators for Luminate®
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
12
Avastin
Lucentis
Eylea
Frequency of
injections
Injections every
4-8 weeks
Injections every
12 weeks
Target patient
population
40-50% of current disease
population (all 3 drugs in
anti-VEGF)
Expanded treatment
population (new MOA)
Anti-angiogenesis: DME & Wet AMD
13. • End-stage patients,
many not responding
to anti-VEGF
• Mean peak BCVA
improvement: 10 letters
(or ~2 lines) 3 months
off-treatment
• 8 patients improved
3+ lines
• Improvements held 3+
months off-treatment
• Corresponding
improvement of BCVA
and CMT
• No patients lost vision
Phase Ib/IIa DME Study: BCVA in All Subjects
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
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9.7
9.3
11.0 11.0 9.1
0
4
8
12
0 Days 30 Days 60 Days 90 Days 120 Days 150 Days
0.0
N=15
30 Days Off
Treatment
60 Days Off
Treatment
90 Days Off
Treatment
Improvement in BCVA (ETDRS
letters converted from Snellen)
14. • Mean peak
improvement in
OCT CMT: 31%
for all subjects
• Improvement
held until Day 150
(3 months off-
treatment)
• Improvements
ranged from 20-
80% reduction in
CMT
Phase Ib/IIa DME Study: OCT CMT in All Subjects
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
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461
439
390 359
387
350
450
550
0 Days 30 Days 60 Days 90 Days 120 Days 150 Days
519
N=15
30 Days Off
Treatment
60 Days Off
Treatment
90 Days Off
Treatment
Central Macular Thickness
(microns)
15. US Phase IIb DME (DEL MAR) Study
DME study comparing 1.0, 2.0, and 3.0 mg Luminate to
Avastin for extended durability
Modeled after phase 1 POC DME study that showed 3+
months durability in monotherapy
105 of 135 subjects enrolled thus far
Efficacy endpoints focused on:
BCVA and OCT central subfield thickness
Increased durability: efficacy 3 or 4 months off tx in monotherapy
vs Avastin
Study completion targeted for Q3 2016
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
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16. Moving Forward With Luminate®
Differentiated mechanisms of action with
unique benefits
Anti-integrin vs. anti-VEGF platform
Increased durability in anti-angiogenesis
Robust vitreolysis
Strong safety profile with >400 human
injections
Top line data in Phase 2b VMT, met primary
endpoint
In multiple Phase 2b US Studies in DME &
PVD ongoing
Copyright 2015 by Allegro Ophthalmics, LLC, All Rights Reserved
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