Super Resolution Microscopy / Localisation Microscopy blinking SPDMphymod with living plant cells expressing GFP fusion proteins SPDM (Spectral Precision Distance Microscopy) SPDM (Spectral Precision Distance Microscopy), the first described localization microscopy technology 1997 is a light optical process of fluorescence microscopy, which allows position, distance and angle measurements on "optically isolated" particles (e.g. molecules) well below the theoretical limit of resolution for light microscopy. This works also in living cells like the plant cells from the image. With this so-called SPDMphymod (physically modifiable fluorophores) technology a single laser wavelength of suitable intensity is sufficient for nanoimaging [35] in contrast to other localization microscopy technologies that need two laser wavelengths when special photo-switchable/photo-activatable fluorescence molecules are used. SPDM is a localization microscopy that achieves an effective optical resolution several times better than the conventional optical resolution, represented by the half-width of the main maximum of the effective point image function. By applying suitable laser optical precision processes, position and distances can be measured with nanometer accuracy between targets with different spectral signatures. Standard fluorescent dyes already successfully used with the SPDMphymod technology are GFP, RFP, YFP, Alexa 488, Alexa 568, Alexa 647, Cy2, Cy3, Atto 488 and fluorescein.

1. Living Plant Cell GFP Super Resolution Microscopy
GFP super resolution microscopy SPDMphymod/localisation microscopy
in a living plant cell expressing GFP fusion proteins
λexc= 488 nm, Localization Accuracy = Pixel size = Effective Optical Resolution ~ 10 nm
Collaboration Christoph Cremer KIP, University of Heidelberg & imb Mainz / Maine and Michael Wassenegger