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Introduction to Biology
Chapter 1, Section 2
History of Scientific Method
Aristotle: Greek philosopher (2300 yag)
Explanations based on logic
2 broad classes
Living (biotic)
Non-living (abiotic)
History of Scientific Method
Common knowledge:
Living things possess a “vital force” that
allows living things to spawn from non-living
sources
Theory of “Spontaneous generation”
History of Scientific Method
Common knowledge:
Living things possess a “vital force” that
allows living things to spawn from non-living
sources
Theory of “Spontaneous generation”
History of Scientific Method
• Francesco Redi (1668) Italian physician
– Challenged spontaneous generation
• Observation: meat left in open air produced maggots
• Common Knowledge: maggots come from dead meat
• Redi’s hypothesis: Flies lay eggs on raw meat, these
eggs turn into maggots.
History of Scientific Method
History of Scientific method
• Redi’s experiment:
• 2 glass containers
– One covered
– One uncovered
• Same type of meat
• Same location
• Same temperature
• Same amount of time
• VARIABLES
History of Scientific Mehtod
• Redi’s Experiment
– Findings
– The container with a cover (gauze to allow equal
air flow) did not generate any maggots.
– Conclusion:
• Maggots only appear on meat that has been exposed to
flies.
History of Scientific Mehtod
• Needham (mid 1700s)
• Combined Redi’s findings with the research of
Leeuwenhoek.
– Leeuwenhoek made the first microscope
• Needham’s experiment
– 2 identical containers
– Both heated, then allowed to sit at same temperature
– Same gravy
History of Scientific Mehtod
• Needham’s Experiment
– Findings: gravy was teeming with microbes
– Conclusion: Microbes come from gravy– spontaneous
generation
History Of Scientific Method
• Spallanzani (Italian Scholar)
–Spallanzani’s Experiment:
• 2 identical containers
• Same gravy
• Heated
– One container sealed immediately
– Other left open to air
• Left to sit for same amount of time
History of Scientific Mehtod
• Spallanzani’s Experiment:
– Findings: Gravy open to air teeming with microbes, while
sealed container had none.
– Conclusion: Microbes too small to see had entered the jar
from the air and reproduced in the gravy.
History of Scientific Method
• Pasteur (1881)
• Pasteur’s Experiment
–Special flask
–Boiled broth
History of scientific Method
• Pasture’s Experiment
• Findings: Broth was free of microorganisms
• Conclusion: So long as broth is protected from
microorganisms broth remains free of life.
History of Scientific Method
• Pasteur’s work lead to other revolutions
in science
–Nature of food spoilage
–Nature of disease
Did I mention History’s Not my thing….
Brain Break!

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Brief History of Scientific Methods

  • 2. History of Scientific Method Aristotle: Greek philosopher (2300 yag) Explanations based on logic 2 broad classes Living (biotic) Non-living (abiotic)
  • 3. History of Scientific Method Common knowledge: Living things possess a “vital force” that allows living things to spawn from non-living sources Theory of “Spontaneous generation”
  • 4. History of Scientific Method Common knowledge: Living things possess a “vital force” that allows living things to spawn from non-living sources Theory of “Spontaneous generation”
  • 5. History of Scientific Method • Francesco Redi (1668) Italian physician – Challenged spontaneous generation • Observation: meat left in open air produced maggots • Common Knowledge: maggots come from dead meat • Redi’s hypothesis: Flies lay eggs on raw meat, these eggs turn into maggots.
  • 7. History of Scientific method • Redi’s experiment: • 2 glass containers – One covered – One uncovered • Same type of meat • Same location • Same temperature • Same amount of time • VARIABLES
  • 8. History of Scientific Mehtod • Redi’s Experiment – Findings – The container with a cover (gauze to allow equal air flow) did not generate any maggots. – Conclusion: • Maggots only appear on meat that has been exposed to flies.
  • 9. History of Scientific Mehtod • Needham (mid 1700s) • Combined Redi’s findings with the research of Leeuwenhoek. – Leeuwenhoek made the first microscope • Needham’s experiment – 2 identical containers – Both heated, then allowed to sit at same temperature – Same gravy
  • 10. History of Scientific Mehtod • Needham’s Experiment – Findings: gravy was teeming with microbes – Conclusion: Microbes come from gravy– spontaneous generation
  • 11. History Of Scientific Method • Spallanzani (Italian Scholar) –Spallanzani’s Experiment: • 2 identical containers • Same gravy • Heated – One container sealed immediately – Other left open to air • Left to sit for same amount of time
  • 12. History of Scientific Mehtod • Spallanzani’s Experiment: – Findings: Gravy open to air teeming with microbes, while sealed container had none. – Conclusion: Microbes too small to see had entered the jar from the air and reproduced in the gravy.
  • 13. History of Scientific Method • Pasteur (1881) • Pasteur’s Experiment –Special flask –Boiled broth
  • 14. History of scientific Method • Pasture’s Experiment • Findings: Broth was free of microorganisms • Conclusion: So long as broth is protected from microorganisms broth remains free of life.
  • 15. History of Scientific Method • Pasteur’s work lead to other revolutions in science –Nature of food spoilage –Nature of disease
  • 16. Did I mention History’s Not my thing…. Brain Break!