4. Advantage of T.L.C.Advantage of T.L.C.
• This is a very easy way to
separate the components.
• TLC is a sensitive method.
• It is feasible to visualize all
components of UV light.
5. • The non-volatile compounds are
separated by the TCL method.
• In comparison to other separation
techniques, very few types of
equipment are used. The
components are separated in a
very short time because the
components will elute rapidly.
6. • The only small sample size is
required in TLC, and it can be in
microlitre.
• The components there in the
complex mixture of samples are
able to easily separate and
recovered by scratching the
plate.
8. Limitation of T.L.C.
• Results obtained from TLC are
difficult to reproduce.
• Only soluble components of
the mixtures are possible.
• This is the only qualitative
analysis possible, not
quantitative.
9. Limitation of T.L.C.
• Usually, it is not automatic.
• TCL works in the open
system, therefore, due to
temperature and humidity
can affect the results.
17. A form of thin
layer
chromatography
in which a
stationary phase
consisting of
silica coated
with silver
nitrate is used to
separate cis- and
trans- fatty acid
derivatives.
Argentation T.L.C.
18. Here, Schematic
separation of methyl
ester derivatives of
unsaturated fatty acids
by Argentation TLC on
silica gel G impregnated
with 10 % (w/w) silver
nitrate.
Plate A: mobile phase
hexane-diethyl ether
(9:1, v/v).
Plate B: as A but
solvents in the ratio 2:3.