In this lecture the technique of artificial insemination in cattle is explained for veterinary students, inseminators, practitioners, and researchers.

1. TECHNIQUE OF ARTIFICIAL
INSEMINATION IN CATTLE
Prof. Dr. G.N Purohit
MVSC, PhD, MNVAS
Head Dept. Veterinary (Gynecology & Obstetrics)
College of veterinary & Animal Science, Bikaner, Raj.
India.
Email: gnpobs@gmail.com
Dr. GN Purohit - Confidential - Content & Video Not Permitted for Commercial Purposes

2. About Me : Professor G N Purohit, MVSc,Ph.D, MNVAS
Head, Department of Veterinary
Gynecology and Obstetrics, Veterinary
College, Bikaner, Rajasthan, India
Dr. GN Purohit - Confidential - Content & Video Not Permitted for Commercial Purposes

3. Todays Topic – Technique of AI in cows.
■ <Topic 1> Preparation for insemination
and sanitation-
■ <Topic 2> -Preparation of AI Gun
■ <Topic 3> -Insemination technique
Dr. GN Purohit - Confidential - Content & Video Not Permitted for Commercial Purposes

4. Preparations for insemination and
sanitation
■ Ensure that the cow to be bred is truly
in heat and the semen to be used has
motility.
■ Restrain the cow first and then thaw the
semen. The restraint area should be familiar
to the cow and free of stressful conditions.
Unnecessary excitement may interfere with
physiological mechanisms important to
achieving a good conception rate.
■ Use proper sanitation

5. Insemination supplies should be kept dry and clean at all times.
Breeding sheaths should be stored in the original package until
used.
Once the insemination device is assembled it must be protected
from contamination and cold shock temperatures.
Materials used to lubricate the rectum should not come in contact
with the vulva region. Lubricants are generally spermicidal.
Avoid using products that are irritating. Alternatively non
spermicidal lubricants can be used.
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6. Avoid raising straws above
the frost line
Use forceps to remove
straws
Place straws in 95º F (350 -370C)
water to thaw for at least 30-45
seconds. Use straws within 15 min
Wipe straw completely dry
before placing in
prewarmed A.I. gun
When all straws are used in
the top goblet, remove and
discard it.
Cut straws ¼" below lab seal at
90º angle.
Common suggestions in preparation of AI guns using frozen semen

7. ■ Locking mechanisms for sheaths of AI Guns
• Split sheaths are designed for use with O-ring guns.
• Non-split sheaths are designed for use with spiral or
Kombicolor guns.
Straw Size
• Sheaths without plastic inserts are exclusively for use with
½ cc straws.
• Sheaths with plastic inserts will accommodate both ¼ and
½ cc straws

8. AI Guns and sheaths
Sheaths are available in bulk or
individual packages. Individually packed
sheaths are better
Three types of A.I. guns

9. Slide sheath over
A.I. gun
Lock sheath and gun
together with snug twist of
the o-ring
Slide sheath underneath
loosened o-ring
Proper seal between straw
and sheath is extremely
important

10. In recent years more precise card type and other electronic
thermal regulators of temperatures for thawing semen at the
most accurate temperatures have become available (1-3).
Similarly for accurate cutting of straws at 900 straw cutters
are available (4)
(1)
(2)
(3) (4)

11. Dr. GN Purohit - Confidential - Content & Video Not Permitted for Commercial Purposes

12. ■ Technique of insemination
■ The vulva region must be thoroughly
wiped clean with a paper towel.
■ To avoid the possibility of entering the
urethral opening on the floor of the vagina,
the insemination gun should be inserted into
the vulva upward at a 30 ̊ to 40 ̊ angle.
■ Place the cervix onto the insemination
gun. Maintain slight forward pressure
on the gun while manipulating the
cervix slightly ahead of the gun.

13. Re-cap – What we learned
■ Ensure that the cow is in heat and restrain the cow first and then thaw the semen
■ Keep the material dry and clean, prevent contact of AI gun spermicidal lubricants
■ Avoid raising straws above frost line and use forceps to remove semen straws
■ Thaw semen at 350C to 370C for 30-45 secs
■ Cut straws straight at 900 angle
■ Split sheaths are used with O ring guns
■ Non-split sheaths are used with spiral or kombicolor guns
■ Sheaths with plastic inserts will accommodate both 0.5 cc
■ and 0.25 cc straws
■ Insert the AI gun at 300 to 400 angle in the vagina and
■ place cervix onto AI gun.
Dr. GN Purohit - Confidential - Content & Video Not Permitted for Commercial Purposes

14. Contact me
Prof. Dr. G.N Purohit
MVSC, PhD, MNVAS
Head Dept. Veterinary (Gynecology & Obstetrics)
College of veterinary & Animal Science, Bikaner, Raj.India.
Email:gnpobs@gmail.com
Linked in : linkedin.com/in/dr-govind-narayan-purohit-13a04a17
YouTube Channel link : https://www.youtube.com/user
/gnpobs61/videos
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