1. BIO-ON srl
via dante 7/b
San Giorgio di Piano – 40016, Bologna - Italy
web: www.bio-on.it
mail: info@bio-on.it
phone: +39051893001
Primary contact: Marco Astorri, CEO
Bio-on’s strength is the ability to produce highly pure PHAs at a competitive
price with comparable mechanical features to PP, PE, PET, HDPE, LDPE.
PHA Polyhydroxyalkanoate is a 100% natural polymer of the polyester family.
To this day, it is considered to be the best polymer in the world.

2. TARGETED PROBLEM
Plastic pollution is "the accumulation in the environment of man-made
plastic products to the point where they create problems for wildlife and
their habitats as well as for human populations”
• Today plastics and synthetic polymers are produced mainly from
hydrocarbons (oil)
 Bio-on can obtain plastic from the production of PHA: a truly biological
plastic, result natural bacterial fermentation of agricultural wastes
• Today plastic recycle processes use large quantities of energy and chemical
products
 Bio-on plastic is 100% biodegradable in bacteriological impure water in few
days

3. TO SOLVE THE PROBLEM
• Bio-on’s aim is to work in the field of the modern biotechnologies applied to widely
used materials. Bio-on intention is to create 100% natural products/solutions based
on renewable resources or agricultural processing waste materials;
• Bio-on offers a polymer technology: PHAs-Polyhydroxyalkanoates, a truly biological
plastic for the full range of strategic applications.
PHAs can compete with traditional plastics, PP, PE, PET, HDPE, LDPE;
• TARGET: Bio-on directly targets the following sectors: food-processing (production and
distribution), medical and pharma pac, electronics, automotive, design and clothing and
others. It provides all players with the technology required to produce and use PHAs in
line with their own characterizations;
• Bio-on’ PHAs production:
– uses agricultural by-products
– does not use any raw material which is considered food (It does not use cereals)
– does not use large quantities of energy nor chemical products for the production of biomaterials
– does not use any of the traditional chemical products used for biopolymer recovery
– modular production unit, easy to connect to existing plants and easy to expand anywhere
– “Zero-miles” philosophy for the use of raw materials
– No genetically modified plants or bacteria are used (OGM Free)
• Bio-on PHAs is 100% biodegradable both in soil and water, fresh and sea water:
non forced biodegradability!

4. MARKET
• The plants currently producing PHA in the Italian head-quarter of Bio-On are on
laboratory scale (6 liters fermentors) and pre-industrial scale (1.400 liters fermentors);
the availability of these facilities allows the fine tuning of the conditions for
fermentation, to direct the production of PHAs with different molecular structures.
• Bio-on issues licences enabling the production of MINERV-PHA™ to produce:
• March 2012: Bio-on has chosen to work with Techint Engineering & Construction to
build plants for the production of PHAs bioplastic. The technology developed over the
last five years by bio-on can now be created worldwide on an industrial scale, with
plants producing 10 thousand tons/year.
• April 2012 : Bio-on and FLOS present the first product in the
world made entirely of PHAs bio plastic, naturally 100%
biodegradable in water. To test this revolutionary material
Bio-on has chosen MISS SISSI, the lamp PHILIPPE STARK
designed for FLOS in 1991.

5. TECHNOLOGY
• From the selection of the bacterial strains isolated by Bio-on to the fermentation of beet sub
products waste comes PHAs (Polyhydroxyalkanoate);
• The “white discs” represent the organic polymer minerv PHAs obtained from sugar beet. These
elements are the result of increased bacteria nourished by the juices of beet (waste molasses):
natural bacterial fermentation in a few dozen hours;
• The next step is the process of ‘recovery’: PHAs are retrieved and separated from the rest of the
organic material, filtered and dried without any solvents;
• All waste material (in small quantity) is re-entered in the initial production cycle in order to feed
new bacterial colonies together with the beet intermediate juices;
• From the PHAs powder extracted from bacteria we obtain PHA pellet for either injection or
extrusion.
A huge work of selection of natural bacteria along the years, avoiding
any use of GMOs, allowed the development of several grades of
PHAs, that are spontaneously accumulated as energy storage by
bacteria feeded with sugars. Polyhydroxyalkanoate PHAs are 100%
bio-based, fully biodegradable plastic materials that can be
considered as the best candidates for traditional oil-based plastics
replacement thanks to their excellent balance of physical, thermal
and mechanical properties. Bio-on produces on its own and licences
world-wide the production of PHA through its patented technology.

6. MARKETING STRATEGY
NEXT STEP - 2013: Industry
• Scale up industrial processes by defining production modules. We create complete Engineering from the laboratory
to the production plant on an industrial scale, with production modules from 5 to 10 thousand tons per year.
• Bio-on offers production technology licensing with design and implementation by qualified industry partners.
• Bio-on has developed a partnership with the architect Enrico Iascone.
• The low environmental impact of PHAs produced by Bio-on have encouraged him to design an eco-friendly
production module in the most sustainable way possible. It is a large, yet reasonably priced project that makes the
optimal use of space for people, materials used and environmental impact. The modular structure is expandable
and can be built anywhere in the world.
• The design incorporates an expandable PHA production capacity of 10 thousand tons per year.
Bio-on 1st Industrial PHAs production plant (2013) from sugar beet waste: Production, Labs, Office, Handling

7. COMPETITORS
PHA COMPETITORS
New Best bio polymers Food Material
Agricultural “waste” Material
standard
GMO
GMO free
Heavy chemical solvent use
NO chemical solvent
Heavy energy use
Low energy use
Manufactory units
IP & Engineering company
High production costs
Low production costs
Slow time to market
Fast time to market
High logistics costs
Assuming a standard end user price per Kg. using the exclusive
method Bio-on to extract the polymer have costs equal to 7%, while GMO Ww territory problem
with the methods known today, which use large amounts of
traditional chemistry, the cost is 70%. NO licensing

8. TEAM
• Mr Marco Astorri, funded Bio-on in 2007 and at present he is President of the
Company. He is the key administrative manager of Bio-On, coordinator of all R&D
activities and in charge of defining the company strategies for industrial development.
• Mr Guido Cicognani, co-funded Bio-on in 2007 and at present he is the vice President
of the Company. He collaborates with Astorri in the administrative, legal and financial
tasks, he is in charge of innovation activities and knowledge exploitation.
• Dr Simone Begotti, Bio-on's head biologist, manages the laboratory research tasks
needed to the develop several grades of PHAs. Dr Begotti is an experienced
biotechnologist with wide skills in the field of biofermentation and biorefinery; in Bio-
on labs he is supported by 4 technical chemist-assistants and strictly collaborates with
fully equipped external research centers for the characterization of the PHA collected
by the fermentors.
• Dr Luca Giamperi, Bio-on's chief materials development. His expertise in the field of
polymer processing supports the team in the identification of the best chemical
structures candidates for satisfying specific needs.
• 10 exclusive advisors experienced in different R&D themes and marketing strategy are
involved in the Bio-on project assuring the quality of the products, their impact in the
near future and the efficiency of the marketing and sales strategy.

9. FINANCIALS
• Bio-on is an autonomous research entity funded in the form of small enterprise; figures
in 2011:
– Employees: 6;
– Turnover: €. 1.154.618,33
– Balance sheet: €. 104.113
• Bio-on’s richness is the know-how regarding the production of PHAs.
• Bio-on has never raised money from banks, friends, family or angels. The company is
self-financed and almost all profit is annualy re-invested in Bio-on’s staff, labs and
facilities.
• Bio-On would like to scale up industrial processes by realising industrial plants for the
production of PHAs, from 5 to 10.000 tons/year, for the full range of strategic
applications
• The expected/estimates revenue are:
– In three years: redouble the actual one and reach around €. 2.300.000;
– In five years: triplicate the actual one and reach around €. 3.500.000
• The capital needed for the next step is difficult to estimate and is linked to different
factors: the marketing strategy is not fully yet decided (JV, investors, alliance, new
partnership). The estimations on number of costumers and the conversion rate will
follow it.

10. SUSTAINABILITY
• Bio-on plastics are plastics in which all carbon is replaced by 100% renewable
feedstocks.
• Bio-on plastics are 100% biodegradable both in soil and water, fresh and sea
water in few days.
• To produce its plastics Bio-on:
– uses agricultural “waste” material (no GMO, no food)
– not use chemical solvents neither in the production nor in the extraction of PHAs
– uses low energy
– “zero-miles” philosophy in the whole chain
– contributes to healthier rural economies
• Minerv PHAs (Bio-on plastic) is certified with the prestigious acknowledgment
of its total and natural biodegradability in water and soil issued by AIB-
VINCOTTE International. Bio-on is the first company in the world producing
PHAs to get this important Certification year 2008.
• Bio-on has designed a low-cost project for the production of PHAs as
sustainable and environmental-friendly as possible that increases the value of
space for people and reduce the environmental impact: the modular
structure is expandable and can be built all over the world.

11. CURRENT STATUS
• April, 2012: MISS SISSI designed by Philippe Stark and
produced with minerv PHAs is presentated world wide in
Milan.
• 2012/2013: Bio-on is finalising the tests on its pilot plan
and is mature enough to begin the 1st industrial plant for
PHAs:
– Dimension: 10.000 mq.
– Production: 10.000 tons/year PHAs from sugar beet waste.
– Eco Building: no pollution.
– New Modular concept: “The same PHAs Building around
the World”.
• The $50,000 First Prize would be invested in the Bio-on pilot plan to co-fund
the purchase of a new dryer in order to conduct new research activities on
PHA characterization.